SSGCID: Target Page

RiafA.10014.a

Lipoprotein releasing system ATP-binding protein LolD

CENTER ID:	RiafA.10014.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.10014.a.B1.GE35835	Full length( RiafA.10014.a )		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.1213
RefSeq:	YP_002845560.1
UniProt:	C3PLH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNTVLILKN ISKHYSQGKI IVRVLDDLNL TVNEGELIAI IGSSGSGKST LLHIAGLLDK PTKGQVIIPN SKYQKYHLIR LHYLGFIYQQ HHLLKDFTAL ENVIMPRLIS GLDQKEAIED ATKILDDLGL GKKLYNMPGE LSGGEKQRVA IARSLINKPK IILADEPTGN LDPKTTNEVF NLFLKVAREQ NTAIIMVTHN HELAHKMDKL YKLKHRLLNI A

NT Sequence

ATGAATAATA CAGTCCTCAT TTTAAAAAAT ATCTCAAAAC ATTACAGCCA AGGCAAAATT ATTGTTAGAG TACTAGATGA TCTTAATCTA ACGGTTAATG AAGGTGAATT AATCGCTATA ATAGGTTCTT CAGGTAGCGG CAAATCAACT TTACTACATA TTGCCGGCTT GCTTGATAAA CCTACAAAAG GTCAAGTCAT CATACCAAAT AGCAAGTATC AAAAATATCA TCTCATTCGT CTTCATTATT TAGGTTTTAT CTATCAACAA CATCACTTAC TTAAAGATTT TACCGCTCTT GAAAATGTTA TTATGCCAAG GCTTATTAGT GGTCTTGATC AAAAAGAAGC AATAGAAGAT GCAACAAAGA TATTAGATGA TTTAGGGCTT GGGAAAAAAC TCTATAATAT GCCTGGAGAA TTATCGGGAG GAGAAAAACA ACGGGTCGCT ATAGCTCGGA GCTTAATTAA TAAGCCGAAA ATTATTTTAG CAGATGAACC GACAGGTAAT TTAGATCCCA AAACTACAAA TGAAGTATTT AATTTATTTT TAAAAGTAGC AAGAGAACAA AATACCGCAA TTATTATGGT AACACATAAC CATGAATTAG CACATAAAAT GGATAAATTA TATAAGCTAA AGCACAGATT ATTAAATATA GCT

Details for RiafA.10014.a.B1.GE35835

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN NTVLILKNIS KHYSQGKIIV RVLDDLNLTV NEGELIAIIG SSGSGKSTLL HIAGLLDKPT KGQVIIPNSK YQKYHLIRLH YLGFIYQQHH LLKDFTALEN VIMPRLISGL DQKEAIEDAT KILDDLGLGK KLYNMPGELS GGEKQRVAIA RSLINKPKII LADEPTGNLD PKTTNEVFNL FLKVAREQNT AIIMVTHNHE LAHKMDKLYK LKHRLLNIA

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgaat aatacagtcc tcattttaaa aaatatctca aaacattaca gccaaggcaa aattattgtt agagtactag atgatcttaa tctaacggtt aatgaaggtg aattaatcgc tataataggt tcttcaggta gcggcaaatc aactttacta catattgccg gcttgcttga taaacctaca aaaggtcaag tcatcatacc aaatagcaag tatcaaaaat atcatctcat tcgtcttcat tatttaggtt ttatctatca acaacatcac ttacttaaag attttaccgc tcttgaaaat gttattatgc caaggcttat tagtggtctt gatcaaaaag aagcaataga agatgcaaca aagatattag atgatttagg gcttgggaaa aaactctata atatgcctgg agaattatcg ggaggagaaa aacaacgggt cgctatagct cggagcttaa ttaataagcc gaaaattatt ttagcagatg aaccgacagg taatttagat cccaaaacta caaatgaagt atttaattta tttttaaaag tagcaagaga acaaaatacc gcaattatta tggtaacaca taaccatgaa ttagcacata aaatggataa attatataag ctaaagcaca gattattaaa tatagcttaa gtgagtaaga taggatccgg ctgcnnnnnc ccccccnnna anngnnnntn nnnnnnnaan nnannnnnnn nntccgctnn ncccnaanna nnnannnnnc nnnntntntt ttatcnnnnn nannnnnnnn nncnnncnnn nncnnnnnnn nnnncncnng nagannnnnn nnnntnnnnc nnnnncnaaa annnanancn nnnnannnnn ngnnnncnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnnn nnnnnnnncn ngcntatnnn nnnnannnan nnnnnnnnnn tg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atacagtcct cattttaaaa aatatctcaa aacattacag ccaaggcaaa attattgtta gagtactaga tgatcttaat ctaacggtta atgaaggtga attaatcgct ataataggtt cttcaggtag cggcaaatca actttactac atattgccgg cttgcttgat aaacctacaa aaggtcaagt catcatacca aatagcaagt atcaaaaata tcatctcatt cgtcttcatt atttaggttt tatctatcaa caacatcact tacttaaaga ttttaccgct cttgaaaatg ttattatgcc aaggcttatt agtggtcttg atcaaaaaga agcaatagaa gatgcaacaa agatattaga tgatttaggg cttgggaaaa aactctataa tatgcctgga gaattatcgg gaggagaaaa acaacgggtc gctatagctc ggagcttaat taataagccg aaaattattt tagcagatga accgacaggt aatttagatc ccaaaactac aaatgaagta tttaatttat ttttaaaagt agcaagagaa caaaataccg caattattat ggtaacacat aaccatgaat tagcacataa aatggataaa ttatataagc taaagcacag attattaaat atagcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t