RiafA.00893.b

Thioredoxin reductase

CENTER ID: RiafA.00893.b
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00893.b.B1.GE35831 Full length( RiafA.00893.b ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.714
RefSeq: YP_002845243.1
UniProt: C3PNJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYNTDVVIIG AGPVGLFAVF QAGMLGMKCH VIDAQEVIGG QCITLYPEKP IYDIPAYPKI AAEELIKQLA LQAAPFNPIY HLNQQAIELN KQDDFFEIKT SKNTLIKSKV IIIAAGAGSF GPNKPSLANI EDFESKSVFY FINDKSKFAG KNIVIAGGGD SAVDWAIALS DIANKIYLVH RRDKFTAAPE SVRQLRHIAE TDKIELITGY QLNALDGNNS ELQSVIVKDL QNNTRKLDAN ILLPFFGLKQ DLGSLANWGL NVKLHHIEVD SSYYQTNIEG IYAIGDIAHY VGKLKLILTG FAEAASSLHH AYSRVFDGKA LHFEYSTTKN TGKRSKSVTK
NT Sequence
ATGTATAATA CTGATGTTGT AATAATAGGT GCCGGTCCGG TTGGATTATT TGCAGTTTTT CAAGCAGGAA TGCTTGGCAT GAAGTGTCAT GTTATTGATG CACAAGAAGT TATCGGCGGT CAGTGTATTA CCCTATATCC TGAAAAGCCT ATTTACGATA TACCGGCATA TCCTAAAATT GCAGCAGAAG AGCTAATAAA ACAGTTAGCA CTTCAAGCAG CTCCTTTTAA CCCTATATAT CATTTAAATC AGCAAGCTAT AGAGCTAAAT AAACAAGATG ATTTTTTTGA AATTAAAACC TCAAAAAACA CTCTTATTAA AAGCAAAGTT ATTATTATTG CAGCAGGAGC AGGTTCATTC GGTCCAAACA AACCGTCCCT TGCGAATATT GAAGATTTTG AAAGCAAGTC AGTTTTTTAT TTCATCAATG ATAAAAGCAA ATTTGCGGGT AAAAATATAG TTATAGCAGG CGGCGGGGAT TCGGCTGTTG ACTGGGCTAT TGCTCTTTCA GATATTGCAA ATAAAATATA TTTAGTACAT AGGCGGGATA AATTTACGGC AGCTCCTGAA AGCGTAAGGC AATTAAGGCA TATTGCAGAA ACCGATAAAA TCGAGTTAAT AACCGGTTAT CAATTAAATG CTTTAGATGG TAATAATAGT GAACTTCAAT CGGTTATAGT TAAAGATTTG CAAAATAATA CTCGCAAATT GGATGCCAAT ATATTACTAC CTTTTTTTGG TTTAAAACAA GATTTAGGTA GCTTAGCAAA TTGGGGTTTA AATGTTAAGC TTCACCATAT TGAAGTAGAC TCTTCCTATT ATCAAACTAA TATAGAAGGT ATTTACGCCA TTGGCGATAT TGCTCATTAT GTTGGTAAGC TTAAGCTGAT TCTTACGGGT TTTGCTGAAG CGGCAAGCAG TCTTCATCAT GCTTATAGTA GAGTATTTGA CGGCAAGGCT CTACATTTTG AGTATTCTAC TACCAAAAAT ACGGGGAAAA GAAGTAAGAG CGTGACTAAA
Details for RiafA.00893.b.B1.GE35831
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 89
Validated AA Sequence
MLGMKCHVID AQEVIGGQCI TLYPEKPIYD IPAYPKIAAE ELIKQLALQA APFNPIYHLN QQAIELNKQD DFFEIKTSKN TLIKSKVIII AAGAGSFGPN KPPLANIEDF ESKSVFYFIN DKSKFAGKNI VIAGGGDSAV DWAIALSDIA NKIYLVHRRD KFTAAPESVR QLRHIAETDK IELITGYQLN ALDGNNSELQ SVIVKDLQNN TRKLDANILL PFFGLKQDLG SLANWGLNVK LHHIEVDSSY YQTNIEGIYA IGDIAHYVGK LKLILTGFAE AASSLHHAYS RVFDGKALHF EYSTTKNTGK RS
Validated NT Sequence
tacttctttt ccccgtattt ttggtagtag aatactcaaa atgtagagcc ttgccgtcaa atactctact ataagcatga tgaagactgc ttgccgcttc ggcaaaaccc gtaagaatca gcttaagctt accaacataa tgagcaatat cgccaatggc gtaaatacct tctatattag tttgataata ggaagagtct acttcaatat ggtgaagctt aacatttaaa ccccaatttg ctaagctacc taaatcttgt tttaaaccaa aaaaaggtag taatatattg gcatccaatt tgcgagtatt attttgcaaa tctttaacta taaccgattg aagttcacta ttattaccat ctaaagcatt taattgataa ccggttatta actcgatttt atcggtttct gcaatatgcc ttaattgcct tacgctttca ggagctgccg taaatttatc ccgcctatgt actaaatata ttttatttgc aatatctgaa agagcaatag cccagtcaac agccgaatcc ccgccgcctg ctataactat atttttaccc gcaaatttgc ttttatcatt gatgaaataa aaaactgact tgctttcaaa atcttcaata ttcgcaaggg gcggtttgtt tggaccgaat gaacctgctc ctgctgcaat aataataact ttgcttttaa taagagtgtt ttttgaggtt ttaatttcaa aaaaatcatc ttgtttattt agctctatag cttgctgatt taaatgatat atagggttaa aaggagctgc ttgaagtgct aactgtttta ttagctcttc tgctgcaatt ttaggatatg ccggtatatc gtaaataggc ttttcaggat atagggtaat acactgaccg ccgataactt cttgtgcatc aataacatga cacttcatgc caagcattcc tgcttgaaaa actgcaaata atccaaccgg accggcacct attattacaa catcagt
Expected Protein Sequence
MAHHHHHHMY NTDVVIIGAG PVGLFAVFQA GMLGMKCHVI DAQEVIGGQC ITLYPEKPIY DIPAYPKIAA EELIKQLALQ AAPFNPIYHL NQQAIELNKQ DDFFEIKTSK NTLIKSKVII IAAGAGSFGP NKPSLANIED FESKSVFYFI NDKSKFAGKN IVIAGGGDSA VDWAIALSDI ANKIYLVHRR DKFTAAPESV RQLRHIAETD KIELITGYQL NALDGNNSEL QSVIVKDLQN NTRKLDANIL LPFFGLKQDL GSLANWGLNV KLHHIEVDSS YYQTNIEGIY AIGDIAHYVG KLKLILTGFA EAASSLHHAY SRVFDGKALH FEYSTTKNTG KRSKSVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata atactgatgt tgtaataata ggtgccggtc cggttggatt atttgcagtt tttcaagcag gaatgcttgg catgaagtgt catgttattg atgcacaaga agttatcggc ggtcagtgta ttaccctata tcctgaaaag cctatttacg atataccggc atatcctaaa attgcagcag aagagctaat aaaacagtta gcacttcaag cagctccttt taaccctata tatcatttaa atcagcaagc tatagagcta aataaacaag atgatttttt tgaaattaaa acctcaaaaa acactcttat taaaagcaaa gttattatta ttgcagcagg agcaggttca ttcggtccaa acaaaccgtc ccttgcgaat attgaagatt ttgaaagcaa gtcagttttt tatttcatca atgataaaag caaatttgcg ggtaaaaata tagttatagc aggcggcggg gattcggctg ttgactgggc tattgctctt tcagatattg caaataaaat atatttagta cataggcggg ataaatttac ggcagctcct gaaagcgtaa ggcaattaag gcatattgca gaaaccgata aaatcgagtt aataaccggt tatcaattaa atgctttaga tggtaataat agtgaacttc aatcggttat agttaaagat ttgcaaaata atactcgcaa attggatgcc aatatattac tacctttttt tggtttaaaa caagatttag gtagcttagc aaattggggt ttaaatgtta agcttcacca tattgaagta gactcttcct attatcaaac taatatagaa ggtatttacg ccattggcga tattgctcat tatgttggta agcttaagct gattcttacg ggttttgctg aagcggcaag cagtcttcat catgcttata gtagagtatt tgacggcaag gctctacatt ttgagtattc tactaccaaa aatacgggga aaagaagtaa gagcgtgact aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat