RiafA.00169.a

Replicative DNA helicase

CENTER ID: RiafA.00169.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00169.a.B1.GE35824 Full length( RiafA.00169.a ) 1 494
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.899
RefSeq: YP_002845364.1
UniProt: C3PNW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARNKINNET NILADNEDNL PIPRVLPSNV QAEQMLLGAI LTNNELLNYV SEFLRDEHFF EPIHQKIYKA IEKITEKGLT ATPITLRSML TQDELFQEVE GAEYLAKLIT MSMMVINPRD YGKIIYDLAI KRNLINIGEE VVNNAYNSSL EVEAKEQIEH AEAKLYDLAS EGLNEKSFTK IGISISESLA SINRAMKNND HIIGISTGLI DLDNKLCGFH NSDLIILAGR PSMGKTAFAI NLALNACNNM RLKNIRDNQE IQSVGFFSLE MSSEQLTTRL LSMCAEIDST SLRTGILGEE KYNRLRKEAN TLSELQFFID DTPALSISAI RTRARRMKRK HNLGILFIDY LQLIRGVSKS ENRVSEISEI TQGLKAIAKE LNIPVIALSQ LSRAVELRED KKPMLSDLRE SGTIEQDADI VMFIYREEYY LTRKEPAAGD AKHAAWLDKL NKVYNIADII VAKHRNGPVG NVPIYYDSQF SKFGNLETRT FNSN
NT Sequence
ATGGCACGTA ATAAAATAAA TAACGAAACA AATATATTAG CTGATAACGA AGATAATTTA CCTATACCAA GGGTTTTACC TTCAAATGTT CAAGCAGAGC AAATGCTACT TGGGGCGATT TTAACCAATA ACGAATTACT AAATTACGTA TCGGAATTTT TACGTGATGA ACATTTTTTT GAACCTATTC ATCAAAAAAT CTATAAGGCA ATTGAGAAAA TTACTGAAAA AGGGCTGACA GCTACACCTA TTACTTTACG TAGTATGTTA ACTCAAGATG AACTATTTCA AGAAGTAGAA GGAGCGGAGT ATTTAGCAAA ATTGATAACT ATGTCAATGA TGGTAATAAA TCCGCGTGAT TATGGTAAAA TAATATATGA TTTAGCAATA AAGCGTAATT TAATAAATAT CGGTGAAGAA GTAGTAAATA ATGCCTATAA TTCTTCATTA GAAGTTGAAG CAAAGGAACA GATTGAACAT GCCGAAGCTA AACTTTACGA TTTAGCTAGT GAAGGCTTGA ATGAAAAAAG TTTTACCAAA ATCGGTATAT CTATTTCAGA ATCACTAGCT AGTATTAATA GGGCTATGAA AAATAATGAT CATATAATCG GTATATCTAC CGGTTTGATT GATCTAGATA ATAAATTATG CGGCTTTCAT AATTCCGATC TTATAATTCT TGCAGGACGT CCGTCTATGG GTAAAACGGC ATTTGCTATA AATCTTGCAC TTAATGCCTG TAATAATATG CGTCTTAAAA ATATTAGAGA TAATCAAGAA ATTCAGTCAG TAGGTTTTTT CTCTTTAGAA ATGTCCTCAG AACAGCTAAC CACACGTCTA CTTTCAATGT GTGCAGAAAT TGATTCTACT TCTCTTCGTA CGGGGATTCT AGGTGAAGAA AAATATAACC GTCTTCGCAA GGAAGCAAAT ACTTTATCGG AATTACAATT TTTTATCGAT GATACCCCTG CTCTATCTAT TTCCGCTATA AGAACAAGAG CTAGAAGAAT GAAACGCAAG CATAATCTCG GTATATTATT TATTGATTAT TTACAATTAA TTAGAGGAGT AAGTAAATCC GAAAATAGAG TTAGCGAGAT TTCAGAAATA ACCCAAGGTT TAAAAGCAAT AGCAAAAGAG TTAAATATTC CGGTTATTGC GTTATCTCAG CTTTCAAGAG CGGTAGAACT ACGTGAGGAC AAAAAACCTA TGCTATCCGA TCTTCGAGAA TCGGGAACTA TAGAACAGGA TGCTGATATA GTAATGTTTA TTTATCGTGA AGAATATTAT TTAACTAGAA AAGAACCGGC AGCAGGCGAT GCTAAACATG CCGCATGGTT AGATAAACTC AACAAAGTAT ATAATATTGC CGATATAATT GTTGCAAAAC ATCGTAACGG ACCAGTTGGA AATGTTCCGA TTTATTATGA TAGTCAATTT TCTAAATTTG GTAATTTGGA GACAAGAACT TTTAACTCTA AT
Details for RiafA.00169.a.B1.GE35824
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 52
Validated AA Sequence
MGKTAFAINL ALNACNNMRL KNIRDNQEIQ SVGFFSLEMS SEQLTTRLLS MCAEIDSTSL RTGILGEEKY NRLRKEANTL SELQFFIDDT PALSISAIRT RARRMKRKHN LGILFIDYLQ LIRGVSKSEN RVSEISEITQ GLKAIAKELN IPVIALSQLS RAVELREDKK PMLSDLRESG TIEQDADIVM FIYREEYYLT RKEPAAGDAK HAAWLDKLNK VYNIADIIVA KHRNGPVGNV PIYYDSQFSK FGNLETRTFN SN
Validated NT Sequence
tttgttagca gccggatcct atcttactca cttaattaga gttaaaagtt cttgtctcca aattaccaaa tttagaaaat tgactatcat aataaatcgg aacatttcca actggtccgt tacgatgttt tgcaacaatt atatcggcaa tattatatac tttgttgagt ttatctaacc atgcggcatg tttagcatcg cctgctgccg gttcttttct agttaaataa tattcttcac gataaataaa cattactata tcagcatcct gttctatagt tcccgattct cgaagatcgg atagcatagg ttttttgtcc tcacgtagtt ctaccgctct tgaaagctga gataacgcaa taaccggaat atttaactct tttgctattg cttttaaacc ttgggttatt tctgaaatct cgctaactct attttcggat ttacttactc ctctaattaa ttgtaaataa tcaataaata atataccgag attatgcttg cgtttcattc ttctagctct tgttcttata gcggaaatag atagagcagg ggtatcatcg ataaaaaatt gtaattccga taaagtattt gcttccttgc gaagacggtt atatttttct tcacctagaa tccccgtacg aagagaagta gaatcaattt ctgcacacat tgaaagtaga cgtgtggtta gctgttctga ggacatttct aaagagaaaa aacctactga ctgaatttct tgattatctc taatattttt aagacgcata ttattacagg cattaagtgc aagatttata gcaaatgccg ttttacccat agacggacgt cctgcaagaa ttataagatc ggaattatga aagccgcata atttattatc tagatcaatc aaaccggtng atataccgnt tatatgatca ttattttttc atagccntat nnnnactanc tagngantct nnnntagata tancnanntt ngntaaaact ttttttcnnt nnnnnnntnc nntanntaan nnnnnnnntt nncnncnnnn nnnnncnnnn nnnnnnnnna nntnnnnnna nnnnnt
Expected Protein Sequence
MAHHHHHHMA RNKINNETNI LADNEDNLPI PRVLPSNVQA EQMLLGAILT NNELLNYVSE FLRDEHFFEP IHQKIYKAIE KITEKGLTAT PITLRSMLTQ DELFQEVEGA EYLAKLITMS MMVINPRDYG KIIYDLAIKR NLINIGEEVV NNAYNSSLEV EAKEQIEHAE AKLYDLASEG LNEKSFTKIG ISISESLASI NRAMKNNDHI IGISTGLIDL DNKLCGFHNS DLIILAGRPS MGKTAFAINL ALNACNNMRL KNIRDNQEIQ SVGFFSLEMS SEQLTTRLLS MCAEIDSTSL RTGILGEEKY NRLRKEANTL SELQFFIDDT PALSISAIRT RARRMKRKHN LGILFIDYLQ LIRGVSKSEN RVSEISEITQ GLKAIAKELN IPVIALSQLS RAVELREDKK PMLSDLRESG TIEQDADIVM FIYREEYYLT RKEPAAGDAK HAAWLDKLNK VYNIADIIVA KHRNGPVGNV PIYYDSQFSK FGNLETRTFN SN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gtaataaaat aaataacgaa acaaatatat tagctgataa cgaagataat ttacctatac caagggtttt accttcaaat gttcaagcag agcaaatgct acttggggcg attttaacca ataacgaatt actaaattac gtatcggaat ttttacgtga tgaacatttt tttgaaccta ttcatcaaaa aatctataag gcaattgaga aaattactga aaaagggctg acagctacac ctattacttt acgtagtatg ttaactcaag atgaactatt tcaagaagta gaaggagcgg agtatttagc aaaattgata actatgtcaa tgatggtaat aaatccgcgt gattatggta aaataatata tgatttagca ataaagcgta atttaataaa tatcggtgaa gaagtagtaa ataatgccta taattcttca ttagaagttg aagcaaagga acagattgaa catgccgaag ctaaacttta cgatttagct agtgaaggct tgaatgaaaa aagttttacc aaaatcggta tatctatttc agaatcacta gctagtatta atagggctat gaaaaataat gatcatataa tcggtatatc taccggtttg attgatctag ataataaatt atgcggcttt cataattccg atcttataat tcttgcagga cgtccgtcta tgggtaaaac ggcatttgct ataaatcttg cacttaatgc ctgtaataat atgcgtctta aaaatattag agataatcaa gaaattcagt cagtaggttt tttctcttta gaaatgtcct cagaacagct aaccacacgt ctactttcaa tgtgtgcaga aattgattct acttctcttc gtacggggat tctaggtgaa gaaaaatata accgtcttcg caaggaagca aatactttat cggaattaca attttttatc gatgataccc ctgctctatc tatttccgct ataagaacaa gagctagaag aatgaaacgc aagcataatc tcggtatatt atttattgat tatttacaat taattagagg agtaagtaaa tccgaaaata gagttagcga gatttcagaa ataacccaag gtttaaaagc aatagcaaaa gagttaaata ttccggttat tgcgttatct cagctttcaa gagcggtaga actacgtgag gacaaaaaac ctatgctatc cgatcttcga gaatcgggaa ctatagaaca ggatgctgat atagtaatgt ttatttatcg tgaagaatat tatttaacta gaaaagaacc ggcagcaggc gatgctaaac atgccgcatg gttagataaa ctcaacaaag tatataatat tgccgatata attgttgcaa aacatcgtaa cggaccagtt ggaaatgttc cgatttatta tgatagtcaa ttttctaaat ttggtaattt ggagacaaga acttttaact ctaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat