RiafA.00159.a

ATP-dependent RNA helicase RhlE

CENTER ID: RiafA.00159.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00159.a.B1.GE35823 Full length( RiafA.00159.a ) 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.1143
RefSeq: YP_002845523.1
UniProt: C3PPC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNFNLSEEL IIALETMNIT EPTEIQKQSI PVAMAGSDIL ASSQTGSGKT LAYLLPLIDS FIKNKTTALI LVPTRELATQ IHSTLNKVTT SYKINSAVLI GGEPMPKQFM QLKKNPKVII GTPGRIIDHL NRGSLKIDRI GITVLDEMDR MLDMGMKEQL EEINKFLPDK RQVLMFSATM PKHIIAVSQK YLNNPVRITV GATNKAAAEI KQESMHVSDK EKFSALTKQL GNREGSVIIF VKTKRSADQL AKMLKYENHT AEAIHGDLSQ RQRERVILSF RKSNHRIMVA TDVAARGLDI PHTQHVINYD LPMCPEDYLH RIGRTGRAGA TGHALSFISP DDVIRWRAID RLVNKGESTP RSEFRSDKNN RKRSFGKRAG GDGKKFNAFD SSRKSTFDGN SYGNKRKKVS
NT Sequence
ATGAAAAATT TTAATTTATC TGAAGAATTA ATTATTGCTC TCGAGACAAT GAATATAACT GAGCCGACTG AAATACAAAA GCAATCGATT CCGGTTGCAA TGGCGGGATC GGACATACTC GCTTCTAGCC AAACAGGTTC AGGCAAAACT CTTGCTTATT TATTGCCGTT AATTGATTCA TTTATTAAAA ATAAAACTAC TGCTTTAATT CTTGTTCCGA CTAGAGAATT AGCAACGCAA ATACACAGTA CTTTAAATAA AGTAACTACA TCCTATAAAA TTAATAGTGC AGTTTTGATA GGCGGTGAAC CGATGCCTAA ACAATTTATG CAATTAAAAA AGAATCCAAA AGTGATTATC GGTACGCCGG GGCGTATTAT TGATCACTTA AATAGGGGAA GTCTAAAAAT TGATCGTATA GGCATAACCG TACTTGACGA AATGGATAGA ATGCTTGATA TGGGGATGAA AGAACAATTA GAAGAAATCA ATAAATTTTT ACCGGACAAA AGACAAGTTT TAATGTTTTC CGCTACTATG CCAAAACATA TTATTGCTGT TTCTCAAAAA TATCTAAACA ATCCGGTACG TATTACGGTA GGTGCTACTA ATAAAGCAGC TGCAGAAATA AAACAGGAAT CAATGCATGT TTCTGATAAA GAAAAATTTA GTGCATTAAC TAAACAACTT GGTAATAGGG AAGGATCGGT CATTATTTTT GTGAAGACTA AACGTTCTGC CGACCAATTA GCTAAAATGT TGAAATATGA AAATCATACA GCAGAAGCTA TACACGGTGA TTTAAGTCAG CGTCAACGTG AAAGAGTAAT TTTATCATTT CGTAAGTCAA ACCATAGAAT AATGGTAGCA ACTGATGTAG CGGCTCGTGG GCTTGATATT CCTCATACAC AGCATGTTAT TAATTATGAT TTACCTATGT GTCCTGAAGA TTATTTACAT AGGATAGGTA GAACGGGTAG AGCAGGGGCT ACCGGACATG CGTTGTCTTT TATTTCTCCT GATGATGTTA TTAGATGGCG TGCAATCGAT CGTTTAGTAA ATAAAGGAGA ATCTACGCCA CGTAGTGAGT TTAGAAGTGA TAAAAATAAT CGTAAAAGAT CATTCGGTAA AAGAGCCGGC GGTGACGGTA AAAAGTTTAA TGCTTTTGAT AGTAGCCGTA AAAGCACCTT TGATGGTAAC AGCTACGGTA ATAAAAGAAA GAAAGTATCG
Details for RiafA.00159.a.B1.GE35823
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 54
Validated AA Sequence
MNITEPTEIQ KQSIPVAMAG SDILASSQTG SGKTLAYLLP LIDSFIKNKT TALILVPTRE LATQIHSTLN KVTTSYKINS AVLIGGEPMP KQFMQLKKNP KVIIGTPGRI IDHLNRGSLK IDRIGITVLD EMDRMLDMGM KEQLEEINKF LPEKRQVLMF SATMPKHIIA VSQKYLNNPV RITVGATNKA AAEIKQESMH VSDKEKFSAL TKQLGNREGS VIIFCED
Validated NT Sequence
accatcaaaa gtgcttttac ggctactatc aaaagcatta aactttttac cgtcaccgcc ggctctttta ccgaatgatc ttttacgatt atttttatca cttctaaact cactacgtgg cgtagattct cctttattta ctaaacgatc gattgcacgc catctaataa catcatcagg agaaataaaa gacaacgcat gtccggtagc ccctgctcta cccgttctac ctatcctatg taaataatct tcaggacaca taggtaaatc ataattaata acatgctgtg tatgaggaat atcaagccca cgagccgcta catcagttgc taccattatt ctatggtttg acttacgaaa tgataaaatt actctttcac gttgacgctg acttaaatca ccgtgtatag cttctgctgt atgattttca tatttcaaca ttttagctaa ttggtcggca gaacgtttag tcttcacaaa aaataatgac cgatccttcc ctattaccaa gttgtttagt taatgcacta aatttttctt tatcagaaac atgcattgat tcctgtttta tttctgcagc tgctttatta gtagcaccta ccgtaatacg taccggattg tttagatatt tttgagaaac agcaataata tgttttggca tagtagcgga aaacattaaa acttgtcttt tttccggtaa aaatttattg atttcttcta attgttcttt catccccata tcaagcattc tatccatttc gtcaagtacg gttatgccta tacgatcaat ttttagactt cccctattta agtgatcaat aatacgcccc ggcgtaccga taatcacttt tggattcttt tttaattgca taaattgttt aggcatcggt tcaccgccta tcaaaactgc actattaatt ttataggatg tagttacttt atttaaagta ctgtgtattt gcgttgctaa ttctctagtc ggaacaagaa ttaaagcagt agttttattt ttaataaatg aatcaattaa cggcaataaa taagcaagag ttttgcctga acctgtttgg ctagaagcga gtatgtccga tcccgccatt gcaaccggaa tcgattgctt ttgtatttca gtcggctcag ttatattcat tgtctcgaga gcaataatta attcttcaga taaattaa
Expected Protein Sequence
MAHHHHHHMK NFNLSEELII ALETMNITEP TEIQKQSIPV AMAGSDILAS SQTGSGKTLA YLLPLIDSFI KNKTTALILV PTRELATQIH STLNKVTTSY KINSAVLIGG EPMPKQFMQL KKNPKVIIGT PGRIIDHLNR GSLKIDRIGI TVLDEMDRML DMGMKEQLEE INKFLPDKRQ VLMFSATMPK HIIAVSQKYL NNPVRITVGA TNKAAAEIKQ ESMHVSDKEK FSALTKQLGN REGSVIIFVK TKRSADQLAK MLKYENHTAE AIHGDLSQRQ RERVILSFRK SNHRIMVATD VAARGLDIPH TQHVINYDLP MCPEDYLHRI GRTGRAGATG HALSFISPDD VIRWRAIDRL VNKGESTPRS EFRSDKNNRK RSFGKRAGGD GKKFNAFDSS RKSTFDGNSY GNKRKKVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa attttaattt atctgaagaa ttaattattg ctctcgagac aatgaatata actgagccga ctgaaataca aaagcaatcg attccggttg caatggcggg atcggacata ctcgcttcta gccaaacagg ttcaggcaaa actcttgctt atttattgcc gttaattgat tcatttatta aaaataaaac tactgcttta attcttgttc cgactagaga attagcaacg caaatacaca gtactttaaa taaagtaact acatcctata aaattaatag tgcagttttg ataggcggtg aaccgatgcc taaacaattt atgcaattaa aaaagaatcc aaaagtgatt atcggtacgc cggggcgtat tattgatcac ttaaataggg gaagtctaaa aattgatcgt ataggcataa ccgtacttga cgaaatggat agaatgcttg atatggggat gaaagaacaa ttagaagaaa tcaataaatt tttaccggac aaaagacaag ttttaatgtt ttccgctact atgccaaaac atattattgc tgtttctcaa aaatatctaa acaatccggt acgtattacg gtaggtgcta ctaataaagc agctgcagaa ataaaacagg aatcaatgca tgtttctgat aaagaaaaat ttagtgcatt aactaaacaa cttggtaata gggaaggatc ggtcattatt tttgtgaaga ctaaacgttc tgccgaccaa ttagctaaaa tgttgaaata tgaaaatcat acagcagaag ctatacacgg tgatttaagt cagcgtcaac gtgaaagagt aattttatca tttcgtaagt caaaccatag aataatggta gcaactgatg tagcggctcg tgggcttgat attcctcata cacagcatgt tattaattat gatttaccta tgtgtcctga agattattta cataggatag gtagaacggg tagagcaggg gctaccggac atgcgttgtc ttttatttct cctgatgatg ttattagatg gcgtgcaatc gatcgtttag taaataaagg agaatctacg ccacgtagtg agtttagaag tgataaaaat aatcgtaaaa gatcattcgg taaaagagcc ggcggtgacg gtaaaaagtt taatgctttt gatagtagcc gtaaaagcac ctttgatggt aacagctacg gtaataaaag aaagaaagta tcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat