RiafA.00157.a

Topoisomerase IV subunit A

CENTER ID: RiafA.00157.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00157.a.B1.GE35822 Full length( RiafA.00157.a ) 1 738
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00157.a.B1.PS01675 Full length( RiafA.00157.a ) 1 738

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.105
RefSeq: YP_002844810.1
UniProt: C3PMA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEAKVENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY TESRMTDICT LLMEDIDKDT VDFRPTYDGA DLEPVIMPAS FPNLLANGSE GIAVGMATNI PPHNLHELCD ALIHLINHPK AEINDIINFV KGPDFPTGGI IIDKAEVINA AYTTGRGSLR VRSRWEKEEL SYGTYQIVVT EIPYQVQKSK LIEQIAILLK DKKIPLVSNI RDESTDIIRL VIEPRDRGCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNILEIL QEFLYHRQNI VTRRSTYLLN KIKHRLEILK GLRIAYLNLD EIIKIIREED APKAIMMERF KLTEIQVEAI LNTRLRSLRK LEEQEIINEH SNLQKQQAIL EEILNNPKEL WKIVKKEIKT VQTKFGLNTV IGARRTSFEE VTLTNQVVDI TAFITKEPIT IICSKMGWVR SLKGHNTDLS TIKYKEGDAE KFILEAYTTD KILIVSSEGR FFTLLADNIS KGKGTGESIK LLVDIGNNDI TNILVYKPDQ LLLLASSVGK GFLVNSNEVM AQTKTGKQIM NVPDGHVCIA CLPVNGDSIA CIGESRKLLV FNIDEIPEMK KGQGVTLQKF KNAKLLDIKI FNKEDGLGWN NNGKVKLEKN IVAFLGKRGS TGKLPPMGFP KNNRFSSY
NT Sequence
ATGAAAGAAG CTAAAGTAGA AAATATTGAC TTTGGTAATG CATTATCAGA GCGTTATCTT GCTTATGCGT TATCAACAAT TATGTCACGT TCGCTTCCTG ACGTACGTGA CGGACTCAAG CCTGTACATC GTAGGTTATT ATACGCAATG CTACAGTTAA GGCTTGAGCC GAATTCCGGC TATAAGAAAT GTGCGAGGGT AGTCGGTGAC GTAATAGGTA AATACCACCC GCACGGTGAT GTGGCAGTGT ATGATACCTT GGTACGCCTT GCTCAGCATT TTTCGTTGCG TTATCCTTTA ATTGATGGGC AGGGTAATTT CGGCTCTATC GACGGTGATA ATGCAGCAGC TATGCGTTAT ACCGAATCAC GTATGACGGA CATATGTACG TTATTAATGG AGGACATCGA TAAAGATACG GTAGATTTTC GCCCTACTTA TGATGGTGCC GATTTAGAAC CGGTAATAAT GCCGGCAAGT TTTCCGAATT TACTAGCTAA TGGTTCTGAA GGGATAGCAG TCGGTATGGC AACCAATATA CCGCCGCATA ATTTACATGA GCTTTGTGAT GCTCTAATAC ATTTAATCAA CCATCCGAAA GCTGAAATTA ACGATATAAT AAACTTTGTC AAAGGTCCTG ATTTTCCGAC CGGCGGCATA ATTATTGATA AAGCTGAAGT TATTAATGCT GCATATACTA CTGGACGCGG TAGTCTTAGA GTAAGAAGCA GATGGGAAAA AGAGGAGCTA AGTTACGGTA CATATCAGAT AGTCGTAACC GAGATACCTT ATCAGGTTCA GAAATCAAAA CTTATAGAAC AAATAGCAAT ATTACTAAAG GATAAAAAAA TTCCCCTTGT TAGCAATATT AGAGATGAAT CAACAGACAT TATAAGGTTG GTTATAGAGC CGAGAGATCG GGGATGTGAT CCGCAAATAG TTATGGAATC TCTATTCAAA CTAACGAATT TAGAAAGCCG CATTCAGTTA AATATGAATG TGATCGGCAG TAATAACGTG CCGAGAGTAA TGAATATTTT AGAGATTTTA CAAGAGTTTC TATATCATAG ACAAAATATT GTTACTCGCA GATCAACTTA TCTTTTAAAC AAAATCAAGC ATCGTTTAGA AATTCTAAAA GGGCTTAGGA TAGCTTATCT AAATCTTGAT GAGATAATTA AAATTATTCG TGAAGAAGAT GCGCCAAAAG CGATAATGAT GGAGCGGTTT AAACTAACCG AAATACAAGT AGAAGCGATA TTAAATACCC GTCTTCGCTC GCTTCGTAAG CTTGAAGAGC AGGAAATTAT TAATGAGCAT AGTAATTTGC AGAAGCAACA AGCTATATTA GAAGAAATTC TAAATAATCC TAAAGAACTA TGGAAAATAG TTAAAAAAGA AATCAAAACA GTACAAACGA AATTTGGATT AAATACGGTA ATAGGAGCAA GGCGTACAAG CTTTGAGGAA GTAACGCTTA CGAATCAAGT AGTTGATATA ACGGCTTTCA TTACTAAAGA GCCGATTACT ATTATCTGCT CAAAAATGGG GTGGGTGCGT TCGTTAAAAG GTCATAACAC CGATTTATCG ACTATCAAAT ATAAAGAAGG TGATGCAGAA AAATTTATTT TAGAAGCTTA CACGACTGAT AAAATATTAA TAGTTAGTTC AGAAGGCAGA TTTTTTACTT TGCTTGCCGA TAATATTTCC AAAGGTAAAG GAACAGGGGA ATCTATAAAA CTACTTGTGG ATATAGGTAA TAACGATATT ACGAATATTT TAGTCTATAA ACCTGATCAG CTTTTATTAC TTGCAAGTAG TGTAGGTAAG GGGTTTTTAG TTAATTCAAA TGAAGTAATG GCTCAAACAA AAACAGGAAA GCAAATTATG AATGTACCGG ATGGTCATGT ATGTATAGCT TGTTTACCTG TAAACGGTGA TAGTATTGCT TGTATCGGTG AAAGTCGTAA ATTACTGGTA TTTAATATAG ATGAAATACC TGAAATGAAA AAAGGGCAAG GTGTAACGCT GCAAAAATTT AAGAATGCTA AGCTTCTAGA TATTAAAATA TTCAATAAAG AAGACGGCTT AGGTTGGAAT AACAATGGAA AAGTAAAACT TGAGAAAAAC ATTGTTGCAT TTTTAGGCAA AAGAGGTAGT ACTGGTAAAT TACCTCCTAT GGGCTTCCCA AAAAATAATA GATTTTCATC ATAT
Details for RiafA.00157.a.B1.GE35822
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 84kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 41
Validated AA Sequence
MAHHHHHHHM KEAKLENIDF GNALSERYLA YALSTIMSRS LPDVRDGLKP VHRRLLYAML QLRLEPNSGY KKCARVVGDV IGKYHPHGDV AVYDTLVRLA QHFSLRYPLI DGQGNFGSID GDNAAAMRYT ESRMTDICTL LMEDIDKDTV DFRPTYDGAD LEPVIMPASF PNLLANGSEG IAVGMATNIP PHNLHELCDA LIHLINHPKA EINDIINFVK GPDFPTGGII IDKAEVINAA YTTGRGSLRV RSRWEKEELS YGTYQIVVTE IPYXVQKSKL IEQIAILLKD KKIPLVSNIR ENQQTL
Validated NT Sequence
attttnnnnn ntttaagaag gagatatacc atggctcacc accaccacca ccaccatatg aaagaagcta aactagaaaa tattgacttt ggtaatgcat tatcagagcg ttatcttgct tatgcgttat caacaattat gtcacgttcg cttcctgacg tacgtgacgg actcaagcct gtacatcgta ggttattata cgcaatgcta cagttaaggc ttgagccgaa ttccggctat aagaaatgtg cgagggtggt cggtgacgta ataggtaaat accacccgca cggtgatgtg gcagtgtatg ataccttggt acgccttgct cagcattttt cgttgcgtta tcctttaatt gatgggcagg gtaatttcgg ctctatcgac ggtgataatg cagcagctat gcgttatacc gaatcacgta tgacggacat atgtacgtta ttaatggagg acatcgataa agatacggta gattttcgcc ctacttatga tggtgccgat ttagaaccgg taataatgcc ggcaagtttt ccgaatttac tagctaatgg ttctgaaggg atagcagtcg gtatggcaac caatataccg ccgcataatt tacatgagct ttgtgatgct ttaatacatt taatcaacca tccgaaagct gaaattaacg atataataaa ctttgtcaaa ggtcctgatt ttccgaccgg cggcataatt attgataaag ctgaagttat taatgctgca tatactactg gacgcggtag tcttagagta agaagcagat gggaaaaaga ggagctaagt tacggtacat atcagatagt cgtaaccgag ataccttatc nggttcagaa atcaaaactt atagaacaaa tagcaatatt actaaaggat aaaaaaattc cccttgttag caatattaga gagaatcaac agacattata annntggtta tagagcnnan agatcggggn ngtgatccnc aaatagtnat ggnntctcta ntcnaactnn cgannttaga agccgcattc agttaannan nnangnnntc ngcagtannn nngncaannn nnnntttnnn nngatttnna nnnnttnnnn nnnnnnnnna cnaaannnnn nnnnnnnnnn naatnnnnnn annnttnnnn nnannnannc ntntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn nnnnntnnna nnntnnt
Expected Protein Sequence
MAHHHHHHMK EAKVENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTDICTLL MEDIDKDTVD FRPTYDGADL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL IHLINHPKAE INDIINFVKG PDFPTGGIII DKAEVINAAY TTGRGSLRVR SRWEKEELSY GTYQIVVTEI PYQVQKSKLI EQIAILLKDK KIPLVSNIRD ESTDIIRLVI EPRDRGCDPQ IVMESLFKLT NLESRIQLNM NVIGSNNVPR VMNILEILQE FLYHRQNIVT RRSTYLLNKI KHRLEILKGL RIAYLNLDEI IKIIREEDAP KAIMMERFKL TEIQVEAILN TRLRSLRKLE EQEIINEHSN LQKQQAILEE ILNNPKELWK IVKKEIKTVQ TKFGLNTVIG ARRTSFEEVT LTNQVVDITA FITKEPITII CSKMGWVRSL KGHNTDLSTI KYKEGDAEKF ILEAYTTDKI LIVSSEGRFF TLLADNISKG KGTGESIKLL VDIGNNDITN ILVYKPDQLL LLASSVGKGF LVNSNEVMAQ TKTGKQIMNV PDGHVCIACL PVNGDSIACI GESRKLLVFN IDEIPEMKKG QGVTLQKFKN AKLLDIKIFN KEDGLGWNNN GKVKLEKNIV AFLGKRGSTG KLPPMGFPKN NRFSSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aagctaaagt agaaaatatt gactttggta atgcattatc agagcgttat cttgcttatg cgttatcaac aattatgtca cgttcgcttc ctgacgtacg tgacggactc aagcctgtac atcgtaggtt attatacgca atgctacagt taaggcttga gccgaattcc ggctataaga aatgtgcgag ggtagtcggt gacgtaatag gtaaatacca cccgcacggt gatgtggcag tgtatgatac cttggtacgc cttgctcagc atttttcgtt gcgttatcct ttaattgatg ggcagggtaa tttcggctct atcgacggtg ataatgcagc agctatgcgt tataccgaat cacgtatgac ggacatatgt acgttattaa tggaggacat cgataaagat acggtagatt ttcgccctac ttatgatggt gccgatttag aaccggtaat aatgccggca agttttccga atttactagc taatggttct gaagggatag cagtcggtat ggcaaccaat ataccgccgc ataatttaca tgagctttgt gatgctctaa tacatttaat caaccatccg aaagctgaaa ttaacgatat aataaacttt gtcaaaggtc ctgattttcc gaccggcggc ataattattg ataaagctga agttattaat gctgcatata ctactggacg cggtagtctt agagtaagaa gcagatggga aaaagaggag ctaagttacg gtacatatca gatagtcgta accgagatac cttatcaggt tcagaaatca aaacttatag aacaaatagc aatattacta aaggataaaa aaattcccct tgttagcaat attagagatg aatcaacaga cattataagg ttggttatag agccgagaga tcggggatgt gatccgcaaa tagttatgga atctctattc aaactaacga atttagaaag ccgcattcag ttaaatatga atgtgatcgg cagtaataac gtgccgagag taatgaatat tttagagatt ttacaagagt ttctatatca tagacaaaat attgttactc gcagatcaac ttatctttta aacaaaatca agcatcgttt agaaattcta aaagggctta ggatagctta tctaaatctt gatgagataa ttaaaattat tcgtgaagaa gatgcgccaa aagcgataat gatggagcgg tttaaactaa ccgaaataca agtagaagcg atattaaata cccgtcttcg ctcgcttcgt aagcttgaag agcaggaaat tattaatgag catagtaatt tgcagaagca acaagctata ttagaagaaa ttctaaataa tcctaaagaa ctatggaaaa tagttaaaaa agaaatcaaa acagtacaaa cgaaatttgg attaaatacg gtaataggag caaggcgtac aagctttgag gaagtaacgc ttacgaatca agtagttgat ataacggctt tcattactaa agagccgatt actattatct gctcaaaaat ggggtgggtg cgttcgttaa aaggtcataa caccgattta tcgactatca aatataaaga aggtgatgca gaaaaattta ttttagaagc ttacacgact gataaaatat taatagttag ttcagaaggc agatttttta ctttgcttgc cgataatatt tccaaaggta aaggaacagg ggaatctata aaactacttg tggatatagg taataacgat attacgaata ttttagtcta taaacctgat cagcttttat tacttgcaag tagtgtaggt aaggggtttt tagttaattc aaatgaagta atggctcaaa caaaaacagg aaagcaaatt atgaatgtac cggatggtca tgtatgtata gcttgtttac ctgtaaacgg tgatagtatt gcttgtatcg gtgaaagtcg taaattactg gtatttaata tagatgaaat acctgaaatg aaaaaagggc aaggtgtaac gctgcaaaaa tttaagaatg ctaagcttct agatattaaa atattcaata aagaagacgg cttaggttgg aataacaatg gaaaagtaaa acttgagaaa aacattgttg catttttagg caaaagaggt agtactggta aattacctcc tatgggcttc ccaaaaaata atagattttc atcatattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for RiafA.00157.a.B1.PS01675
PURIFICATION DATe: 11/16/2012
CONCENTRATION: 28mg/ml
OBSERVED MW: 82kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 41
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM KEAKLENIDF GNALSERYLA YALSTIMSRS LPDVRDGLKP VHRRLLYAML QLRLEPNSGY KKCARVVGDV IGKYHPHGDV AVYDTLVRLA QHFSLRYPLI DGQGNFGSID GDNAAAMRYT ESRMTDICTL LMEDIDKDTV DFRPTYDGAD LEPVIMPASF PNLLANGSEG IAVGMATNIP PHNLHELCDA LIHLINHPKA EINDIINFVK GPDFPTGGII IDKAEVINAA YTTGRGSLRV RSRWEKEELS YGTYQIVVTE IPYXVQKSKL IEQIAILLKD KKIPLVSNIR ENQQTL
Validated NT Sequence
attttnnnnn ntttaagaag gagatatacc atggctcacc accaccacca ccaccatatg aaagaagcta aactagaaaa tattgacttt ggtaatgcat tatcagagcg ttatcttgct tatgcgttat caacaattat gtcacgttcg cttcctgacg tacgtgacgg actcaagcct gtacatcgta ggttattata cgcaatgcta cagttaaggc ttgagccgaa ttccggctat aagaaatgtg cgagggtggt cggtgacgta ataggtaaat accacccgca cggtgatgtg gcagtgtatg ataccttggt acgccttgct cagcattttt cgttgcgtta tcctttaatt gatgggcagg gtaatttcgg ctctatcgac ggtgataatg cagcagctat gcgttatacc gaatcacgta tgacggacat atgtacgtta ttaatggagg acatcgataa agatacggta gattttcgcc ctacttatga tggtgccgat ttagaaccgg taataatgcc ggcaagtttt ccgaatttac tagctaatgg ttctgaaggg atagcagtcg gtatggcaac caatataccg ccgcataatt tacatgagct ttgtgatgct ttaatacatt taatcaacca tccgaaagct gaaattaacg atataataaa ctttgtcaaa ggtcctgatt ttccgaccgg cggcataatt attgataaag ctgaagttat taatgctgca tatactactg gacgcggtag tcttagagta agaagcagat gggaaaaaga ggagctaagt tacggtacat atcagatagt cgtaaccgag ataccttatc nggttcagaa atcaaaactt atagaacaaa tagcaatatt actaaaggat aaaaaaattc cccttgttag caatattaga gagaatcaac agacattata annntggtta tagagcnnan agatcggggn ngtgatccnc aaatagtnat ggnntctcta ntcnaactnn cgannttaga agccgcattc agttaannan nnangnnntc ngcagtannn nngncaannn nnnntttnnn nngatttnna nnnnttnnnn nnnnnnnnna cnaaannnnn nnnnnnnnnn naatnnnnnn annnttnnnn nnannnannc ntntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn nnnnntnnna nnntnnt
Expressed Protein Sequence
MAHHHHHHMK EAKVENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTDICTLL MEDIDKDTVD FRPTYDGADL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL IHLINHPKAE INDIINFVKG PDFPTGGIII DKAEVINAAY TTGRGSLRVR SRWEKEELSY GTYQIVVTEI PYQVQKSKLI EQIAILLKDK KIPLVSNIRD ESTDIIRLVI EPRDRGCDPQ IVMESLFKLT NLESRIQLNM NVIGSNNVPR VMNILEILQE FLYHRQNIVT RRSTYLLNKI KHRLEILKGL RIAYLNLDEI IKIIREEDAP KAIMMERFKL TEIQVEAILN TRLRSLRKLE EQEIINEHSN LQKQQAILEE ILNNPKELWK IVKKEIKTVQ TKFGLNTVIG ARRTSFEEVT LTNQVVDITA FITKEPITII CSKMGWVRSL KGHNTDLSTI KYKEGDAEKF ILEAYTTDKI LIVSSEGRFF TLLADNISKG KGTGESIKLL VDIGNNDITN ILVYKPDQLL LLASSVGKGF LVNSNEVMAQ TKTGKQIMNV PDGHVCIACL PVNGDSIACI GESRKLLVFN IDEIPEMKKG QGVTLQKFKN AKLLDIKIFN KEDGLGWNNN GKVKLEKNIV AFLGKRGSTG KLPPMGFPKN NRFSSY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG AAGCTAAAGT AGAAAATATT GACTTTGGTA ATGCATTATC AGAGCGTTAT CTTGCTTATG CGTTATCAAC AATTATGTCA CGTTCGCTTC CTGACGTACG TGACGGACTC AAGCCTGTAC ATCGTAGGTT ATTATACGCA ATGCTACAGT TAAGGCTTGA GCCGAATTCC GGCTATAAGA AATGTGCGAG GGTAGTCGGT GACGTAATAG GTAAATACCA CCCGCACGGT GATGTGGCAG TGTATGATAC CTTGGTACGC CTTGCTCAGC ATTTTTCGTT GCGTTATCCT TTAATTGATG GGCAGGGTAA TTTCGGCTCT ATCGACGGTG ATAATGCAGC AGCTATGCGT TATACCGAAT CACGTATGAC GGACATATGT ACGTTATTAA TGGAGGACAT CGATAAAGAT ACGGTAGATT TTCGCCCTAC TTATGATGGT GCCGATTTAG AACCGGTAAT AATGCCGGCA AGTTTTCCGA ATTTACTAGC TAATGGTTCT GAAGGGATAG CAGTCGGTAT GGCAACCAAT ATACCGCCGC ATAATTTACA TGAGCTTTGT GATGCTCTAA TACATTTAAT CAACCATCCG AAAGCTGAAA TTAACGATAT AATAAACTTT GTCAAAGGTC CTGATTTTCC GACCGGCGGC ATAATTATTG ATAAAGCTGA AGTTATTAAT GCTGCATATA CTACTGGACG CGGTAGTCTT AGAGTAAGAA GCAGATGGGA AAAAGAGGAG CTAAGTTACG GTACATATCA GATAGTCGTA ACCGAGATAC CTTATCAGGT TCAGAAATCA AAACTTATAG AACAAATAGC AATATTACTA AAGGATAAAA AAATTCCCCT TGTTAGCAAT ATTAGAGATG AATCAACAGA CATTATAAGG TTGGTTATAG AGCCGAGAGA TCGGGGATGT GATCCGCAAA TAGTTATGGA ATCTCTATTC AAACTAACGA ATTTAGAAAG CCGCATTCAG TTAAATATGA ATGTGATCGG CAGTAATAAC GTGCCGAGAG TAATGAATAT TTTAGAGATT TTACAAGAGT TTCTATATCA TAGACAAAAT ATTGTTACTC GCAGATCAAC TTATCTTTTA AACAAAATCA AGCATCGTTT AGAAATTCTA AAAGGGCTTA GGATAGCTTA TCTAAATCTT GATGAGATAA TTAAAATTAT TCGTGAAGAA GATGCGCCAA AAGCGATAAT GATGGAGCGG TTTAAACTAA CCGAAATACA AGTAGAAGCG ATATTAAATA CCCGTCTTCG CTCGCTTCGT AAGCTTGAAG AGCAGGAAAT TATTAATGAG CATAGTAATT TGCAGAAGCA ACAAGCTATA TTAGAAGAAA TTCTAAATAA TCCTAAAGAA CTATGGAAAA TAGTTAAAAA AGAAATCAAA ACAGTACAAA CGAAATTTGG ATTAAATACG GTAATAGGAG CAAGGCGTAC AAGCTTTGAG GAAGTAACGC TTACGAATCA AGTAGTTGAT ATAACGGCTT TCATTACTAA AGAGCCGATT ACTATTATCT GCTCAAAAAT GGGGTGGGTG CGTTCGTTAA AAGGTCATAA CACCGATTTA TCGACTATCA AATATAAAGA AGGTGATGCA GAAAAATTTA TTTTAGAAGC TTACACGACT GATAAAATAT TAATAGTTAG TTCAGAAGGC AGATTTTTTA CTTTGCTTGC CGATAATATT TCCAAAGGTA AAGGAACAGG GGAATCTATA AAACTACTTG TGGATATAGG TAATAACGAT ATTACGAATA TTTTAGTCTA TAAACCTGAT CAGCTTTTAT TACTTGCAAG TAGTGTAGGT AAGGGGTTTT TAGTTAATTC AAATGAAGTA ATGGCTCAAA CAAAAACAGG AAAGCAAATT ATGAATGTAC CGGATGGTCA TGTATGTATA GCTTGTTTAC CTGTAAACGG TGATAGTATT GCTTGTATCG GTGAAAGTCG TAAATTACTG GTATTTAATA TAGATGAAAT ACCTGAAATG AAAAAAGGGC AAGGTGTAAC GCTGCAAAAA TTTAAGAATG CTAAGCTTCT AGATATTAAA ATATTCAATA AAGAAGACGG CTTAGGTTGG AATAACAATG GAAAAGTAAA ACTTGAGAAA AACATTGTTG CATTTTTAGG CAAAAGAGGT AGTACTGGTA AATTACCTCC TATGGGCTTC CCAAAAAATA ATAGATTTTC ATCATATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT