SSGCID: Target Page

RiafA.00148.b

Tyrosine recombinase XerD

CENTER ID:	RiafA.00148.b
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.00148.b.B1.GE35821	Full length( RiafA.00148.b )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.00148.b.B1.PS01653	Full length( RiafA.00148.b )		1	306

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.551
RefSeq:	YP_002845128.1
UniProt:	C3PN75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGFIAQFLEM LLAERALSKN SILSYKRDLF DFQNYLAKHK LSELNITTEN IRDWIEYLAS NDLQARSINR KISTIKSYYE FLISENHTAF NPVLNVDLPK YQNKLPEILS IAQIKSLLEH CSQDNSPEGI RLNAMIHLLY ASGLRVSELV SLKLADILTN KTSKGEVRKI FSVLGKGNKE RVIVINEQAV ISIAKYLAIR GVFVNKAKPR NLIYLFPSSA LAGYMTRQNF AILLKSAALY AGLNPEYISP HILRHSFASH LLEGGADLRV IQELLGHADI STTQIYTHLQ TNHLKKALLH HPLNKN

NT Sequence

ATGGGATTTA TTGCGCAATT CCTAGAAATG CTTTTAGCCG AGAGAGCATT GTCAAAAAAT TCTATACTTA GCTATAAGCG TGACTTATTC GATTTTCAAA ATTATCTTGC TAAACACAAA TTATCTGAAT TAAATATTAC TACTGAGAAT ATTAGAGACT GGATTGAGTA TCTAGCAAGT AATGATTTGC AAGCACGCTC AATCAACAGA AAAATCTCAA CTATAAAAAG TTATTATGAA TTTTTAATTA GCGAAAACCA TACCGCGTTT AATCCGGTTC TTAACGTAGA TTTGCCCAAA TATCAAAATA AACTTCCCGA AATATTATCT ATAGCTCAAA TTAAATCGTT ACTTGAACAT TGCTCTCAAG ATAATTCTCC TGAAGGTATC AGGCTTAATG CTATGATTCA TTTGCTTTAT GCTAGCGGTC TTAGAGTCTC GGAGCTTGTT AGTCTTAAGC TTGCTGATAT TCTGACTAAT AAAACGTCTA AAGGAGAAGT AAGAAAAATA TTTTCGGTAC TTGGTAAAGG TAATAAGGAA AGAGTCATAG TAATAAATGA ACAGGCAGTT ATCAGTATTG CTAAATATCT TGCAATTAGA GGTGTTTTTG TGAATAAAGC TAAACCCAGA AATCTAATTT ATTTATTTCC TTCATCAGCT TTAGCGGGTT ATATGACTAG GCAAAATTTT GCTATTCTGT TAAAATCTGC TGCCCTTTAT GCAGGACTTA ACCCTGAGTA TATTTCTCCG CATATATTAC GTCACAGTTT TGCAAGTCAT TTGCTTGAGG GCGGAGCAGA CTTAAGAGTA ATTCAAGAGC TGCTTGGTCA CGCTGATATA TCTACTACCC AAATATATAC TCATCTTCAA ACTAATCATC TCAAAAAAGC ATTGTTGCAT CATCCTCTCA ATAAAAAT

Details for RiafA.00148.b.B1.GE35821

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGFIAQFLEM LLAERALSKN SILSYKRDLF DFQNYLAKHK LSELSITTEN IRDWIEYLAS NDLQARSINR KISTIKSYYE FLISENHTAF NPVLNVDLPK YQNKLPEILS IAQIKSLLEH CSQDNSPEGI RLNAMIHLLY ASGLRVSELV SLKLADILTN KTSKGEVRKI FSVLGKGNKE RVIVINEQAV ISIAKYLAIR GVFVNKAKPR NLIYLFPSSA LAGYMTRQNF AILLKSAALY AGLNPEYISP HILRHSFASH LLEGGADLRV IQELLGHADI STTQIYTHLQ TNHLKKALLH HPLNKN

Validated NT Sequence

cctatcttac tcncttaatt tttattgaga ggatgatgca acaatgcttt tttgagatga ttagtttgaa gatgagtata tatttgggta gtagatatat cagcgtgacc aagcagctct tgaattactc ttaagtctgc tccgccctca agcaaatgac ttgcaaaact gtgacgtaat atatgcggag aaatatactc agggttaagt cctgcataaa gggcagcaga ttttaacaga atagcaaaat tttgcctagt catataaccc gctaaagctg atgaaggaaa taaataaatt agatttctgg gtttagcttt attcacaaaa acacctctaa ttgcaagata tttagcaata ctgataactg cctgttcatt tattactatg actctttcct tattaccttt accaagtacc gaaaatattt ttcttacttc tcctttagac gttttattag tcagaatatc agcaagctta agactaacaa gctccgagac tctaagaccg ctagcataaa gcaaatgaat catagcatta agcctgatac cttcaggaga attatcttga gagcaatgtt caagtaacga tttaatttga gctatagata atatttcggg aagtttattt tgatatttgg gcaaatctac gttaagaacc ggattaaacg cggtatggtt ttcgctaatt aaaaattcat aataactttt tatagttgag atttttctgt tgattgagcg tgcttgcaaa tcattacttg ctagatactc aatccagtct ctaatattct cagtagtaat acttaattcg gataatttgt gtttagcaag ataattttga aaatcgaata agtcacgctt atagctaagt atagaatttt ttgacaatgc tctctcggct aaaagcattt ctaggaattg cgcaataaat cccatatggt ggtggtg

Expected Protein Sequence

MAHHHHHHMG FIAQFLEMLL AERALSKNSI LSYKRDLFDF QNYLAKHKLS ELNITTENIR DWIEYLASND LQARSINRKI STIKSYYEFL ISENHTAFNP VLNVDLPKYQ NKLPEILSIA QIKSLLEHCS QDNSPEGIRL NAMIHLLYAS GLRVSELVSL KLADILTNKT SKGEVRKIFS VLGKGNKERV IVINEQAVIS IAKYLAIRGV FVNKAKPRNL IYLFPSSALA GYMTRQNFAI LLKSAALYAG LNPEYISPHI LRHSFASHLL EGGADLRVIQ ELLGHADIST TQIYTHLQTN HLKKALLHHP LNKN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggat ttattgcgca attcctagaa atgcttttag ccgagagagc attgtcaaaa aattctatac ttagctataa gcgtgactta ttcgattttc aaaattatct tgctaaacac aaattatctg aattaaatat tactactgag aatattagag actggattga gtatctagca agtaatgatt tgcaagcacg ctcaatcaac agaaaaatct caactataaa aagttattat gaatttttaa ttagcgaaaa ccataccgcg tttaatccgg ttcttaacgt agatttgccc aaatatcaaa ataaacttcc cgaaatatta tctatagctc aaattaaatc gttacttgaa cattgctctc aagataattc tcctgaaggt atcaggctta atgctatgat tcatttgctt tatgctagcg gtcttagagt ctcggagctt gttagtctta agcttgctga tattctgact aataaaacgt ctaaaggaga agtaagaaaa atattttcgg tacttggtaa aggtaataag gaaagagtca tagtaataaa tgaacaggca gttatcagta ttgctaaata tcttgcaatt agaggtgttt ttgtgaataa agctaaaccc agaaatctaa tttatttatt tccttcatca gctttagcgg gttatatgac taggcaaaat tttgctattc tgttaaaatc tgctgccctt tatgcaggac ttaaccctga gtatatttct ccgcatatat tacgtcacag ttttgcaagt catttgcttg agggcggagc agacttaaga gtaattcaag agctgcttgg tcacgctgat atatctacta cccaaatata tactcatctt caaactaatc atctcaaaaa agcattgttg catcatcctc tcaataaaaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for RiafA.00148.b.B1.PS01653

PURIFICATION DATe:	10/9/2012
CONCENTRATION:	15.22mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	22µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAT TTATTGCGCA ATTCCTAGAA ATGCTTTTAG CCGAGAGAGC ATTGTCAAAA AATTCTATAC TTAGCTATAA GCGTGACTTA TTCGATTTTC AAAATTATCT TGCTAAACAC AAATTATCTG AATTAAATAT TACTACTGAG AATATTAGAG ACTGGATTGA GTATCTAGCA AGTAATGATT TGCAAGCACG CTCAATCAAC AGAAAAATCT CAACTATAAA AAGTTATTAT GAATTTTTAA TTAGCGAAAA CCATACCGCG TTTAATCCGG TTCTTAACGT AGATTTGCCC AAATATCAAA ATAAACTTCC CGAAATATTA TCTATAGCTC AAATTAAATC GTTACTTGAA CATTGCTCTC AAGATAATTC TCCTGAAGGT ATCAGGCTTA ATGCTATGAT TCATTTGCTT TATGCTAGCG GTCTTAGAGT CTCGGAGCTT GTTAGTCTTA AGCTTGCTGA TATTCTGACT AATAAAACGT CTAAAGGAGA AGTAAGAAAA ATATTTTCGG TACTTGGTAA AGGTAATAAG GAAAGAGTCA TAGTAATAAA TGAACAGGCA GTTATCAGTA TTGCTAAATA TCTTGCAATT AGAGGTGTTT TTGTGAATAA AGCTAAACCC AGAAATCTAA TTTATTTATT TCCTTCATCA GCTTTAGCGG GTTATATGAC TAGGCAAAAT TTTGCTATTC TGTTAAAATC TGCTGCCCTT TATGCAGGAC TTAACCCTGA GTATATTTCT CCGCATATAT TACGTCACAG TTTTGCAAGT CATTTGCTTG AGGGCGGAGC AGACTTAAGA GTAATTCAAG AGCTGCTTGG TCACGCTGAT ATATCTACTA CCCAAATATA TACTCATCTT CAAACTAATC ATCTCAAAAA AGCATTGTTG CATCATCCTC TCAATAAAAA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT