RiafA.00148.a

Tyrosine recombinase xerC

CENTER ID: RiafA.00148.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00148.a.B1.GE35820 Full length( RiafA.00148.a ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.1422
RefSeq: YP_002845681.1
UniProt: C3PLU8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDTSIQALI NKWQKYLVLQ RNYSNHTVIS YNNDLKHFLE FMNYYNSELV TINHIKTADI RLIRSWLAKR NCDNFTASSI SRGLSAVKNF YRFLEKTTQL NSHIIFSIKS PKKTKLLPKA LSEDDVVISL EHIEEYGNVK WIELRNKALL VLIYASGLRI SEALSITKLH LQNLEFIRII GKGSKERIIP WLPIAKNLIT QYLEILPYKL GDNEPIFRGK QGKKLQPPVF NRELIKLKHF YGLPQHLTAH SFRHSFASHL LEHGADLRSL QELLGHKSLS TTQSYTKTSI KHLEAVYTTA YPIKK
NT Sequence
ATGTTAGATA CATCAATTCA AGCATTAATA AATAAGTGGC AAAAATACCT CGTTTTGCAA AGAAATTACT CTAATCATAC AGTAATTTCT TATAATAATG ACCTTAAGCA TTTTCTTGAG TTTATGAATT ATTATAATTC AGAGCTTGTA ACGATAAATC ATATTAAAAC CGCAGATATC AGATTAATCC GAAGTTGGCT TGCAAAACGT AACTGTGATA ATTTTACCGC TTCTTCAATT TCACGTGGTT TATCTGCAGT AAAAAATTTT TATAGGTTTT TAGAGAAAAC AACACAATTA AATAGTCATA TAATTTTTTC TATAAAATCT CCTAAAAAGA CTAAATTGTT ACCAAAAGCT TTATCGGAAG ATGATGTAGT AATATCACTT GAACATATTG AAGAATACGG GAATGTTAAG TGGATAGAAC TTAGAAATAA AGCCCTACTT GTCCTTATAT ATGCTTCAGG TCTACGTATA TCGGAAGCAT TATCAATTAC GAAACTTCAT CTACAAAATC TAGAATTTAT AAGAATAATA GGTAAAGGTA GTAAAGAAAG AATAATTCCG TGGTTACCGA TTGCTAAAAA TTTAATTACC CAATATTTAG AAATATTGCC ATATAAGTTA GGTGATAATG AACCCATATT TAGAGGAAAG CAGGGTAAGA AATTGCAACC ACCGGTATTT AATCGTGAAT TAATTAAATT AAAGCATTTT TATGGTTTAC CGCAGCATTT AACTGCTCAT TCATTTAGAC ATAGTTTTGC TTCACATTTG CTTGAGCATG GTGCAGATTT ACGCTCTCTT CAAGAACTAT TAGGTCATAA AAGTCTATCT ACTACACAAA GCTATACTAA AACAAGTATA AAGCATTTAG AAGCTGTATA TACTACCGCA TATCCAATTA AAAAA
Details for RiafA.00148.a.B1.GE35820
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML DTSIQALINK WQKYLVLQRN YSNHTVISYN NDLKHFLEFM NYYNSELVTI NHIKTADIRL IRSWLAKRNC DNFTASSISR GLSAVKNFYR FLEKTTQLNS HIIFSIKSPK KTKLLPKALS EDDVVISLEH IEEYGNVKWI ELRNRALLVL IYASGLRISE ALSITKLHLQ NLEFIRIIGK GSKERIIPWL PIAKNLITQY LEILPYKLGD NEPIFRGKQG KKLQPPVFNR ELIKLKHFYG LPQHLTAHSF RHSFASHLLE HGADLRSLQE LLGHKSLSTT QSYTKTSIKH LEAXYTTAYP XKNK
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatgttagat acatcaattc aagcattaat aaataagtgg caaaaatacc tcgttttgca aagaaattac tctaatcata cagtaatttc ttataataat gaccttaagc attttcttga gtttatgaat tattataatt cagagcttgt aacgataaat catattaaaa ccgcagatat cagattaatc cgaagttggc ttgcaaaacg taactgtgat aattttaccg cttcttcaat ttcacgtggt ttatctgcag taaaaaattt ttataggttt ttagagaaaa caacacaatt aaatagtcat ataatttttt ctataaaatc tcctaaaaag actaaattgt taccaaaagc tttatcggaa gatgatgtag taatatcact tgaacatatt gaagaatacg ggaatgttaa gtggatagaa cttagaaata gagccctact tgtccttata tatgcttcag gtctacgtat atcggaagca ttatcaatta cgaaacttca tctacaaaat ctagaattta taagaataat aggtaaaggt agtaaagaaa gaataattcc gtggttaccg attgctaaaa atttaattac ccaatattta gaaatattgc catataagtt aggtgataat gaacccatat ttagaggaaa gcagggtaag aaattgcaac caccggtatt taatcgtgaa ttaattaaat taaagcattt ttatggttta ccgcagcatt taactgctca ttcatttaga catagttttg cttcacattt gcttgagcat ggtgcagatt tacgctctct tcaagaacta ttaggtcata aaagtctatc tactacacaa agctatacta aaacaagtat aaagcattta gaagctgnat atactaccgc atatccnntn aaaaataagt gagnanntag naaaaaaann nnaaaa
Expected Protein Sequence
MAHHHHHHML DTSIQALINK WQKYLVLQRN YSNHTVISYN NDLKHFLEFM NYYNSELVTI NHIKTADIRL IRSWLAKRNC DNFTASSISR GLSAVKNFYR FLEKTTQLNS HIIFSIKSPK KTKLLPKALS EDDVVISLEH IEEYGNVKWI ELRNKALLVL IYASGLRISE ALSITKLHLQ NLEFIRIIGK GSKERIIPWL PIAKNLITQY LEILPYKLGD NEPIFRGKQG KKLQPPVFNR ELIKLKHFYG LPQHLTAHSF RHSFASHLLE HGADLRSLQE LLGHKSLSTT QSYTKTSIKH LEAVYTTAYP IKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag atacatcaat tcaagcatta ataaataagt ggcaaaaata cctcgttttg caaagaaatt actctaatca tacagtaatt tcttataata atgaccttaa gcattttctt gagtttatga attattataa ttcagagctt gtaacgataa atcatattaa aaccgcagat atcagattaa tccgaagttg gcttgcaaaa cgtaactgtg ataattttac cgcttcttca atttcacgtg gtttatctgc agtaaaaaat ttttataggt ttttagagaa aacaacacaa ttaaatagtc atataatttt ttctataaaa tctcctaaaa agactaaatt gttaccaaaa gctttatcgg aagatgatgt agtaatatca cttgaacata ttgaagaata cgggaatgtt aagtggatag aacttagaaa taaagcccta cttgtcctta tatatgcttc aggtctacgt atatcggaag cattatcaat tacgaaactt catctacaaa atctagaatt tataagaata ataggtaaag gtagtaaaga aagaataatt ccgtggttac cgattgctaa aaatttaatt acccaatatt tagaaatatt gccatataag ttaggtgata atgaacccat atttagagga aagcagggta agaaattgca accaccggta tttaatcgtg aattaattaa attaaagcat ttttatggtt taccgcagca tttaactgct cattcattta gacatagttt tgcttcacat ttgcttgagc atggtgcaga tttacgctct cttcaagaac tattaggtca taaaagtcta tctactacac aaagctatac taaaacaagt ataaagcatt tagaagctgt atatactacc gcatatccaa ttaaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat