SSGCID: Target Page

RiafA.00137.a

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID:	RiafA.00137.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.00137.a.B1.GE35818	Full length( RiafA.00137.a )		1	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.370
RefSeq:	YP_002844997.1
UniProt:	C3PMU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLLELKKTN QTLETIHFIG IGGVGMSGIA EILYNLGYKV QGSDLVENYN TKRLESYGIK IFLGHAEQNI TNVSYVVISS AINPKNPEIK EALERKIPII RRADMLAELM RLKCSVAVSG SHGKTTTTSL VACLFEAAGL CPTVINGGII NNKSTNAYLG SSNYLIAEAD ESDATFIHIP STIAIITNID PEHLDYYRDF ETLIGAFRSF ITNLPFYGFA VCCIDHKIVR KLVDDITERK IVTYGIDSED AHIIAFNINT DIASSTFDVK ISLPNVLGTT IIEKITIPTP GRHNILNSLA AIAVGIELDF GIKAIKNGFN NFKGVKRRFT KVAEYNNASI IDDYAHHPEE IKATLATAKN IANKQNGKVI AIFQPHRYSR MQYLFDDFMF CFADADILYI TDIYAAGENP IEGITGRNLV DKITKRKHHD KANFLAELDD AVGVIIDNAA SGDMIIMMGA GNISSFANEL EGRLSSRGFS CHTVV

NT Sequence

ATGCTGTTAC TTGAATTAAA AAAAACTAAT CAAACCTTAG AAACTATACA TTTTATAGGT ATCGGCGGCG TTGGCATGAG CGGTATTGCC GAGATATTAT ATAATCTAGG TTATAAAGTA CAAGGTTCCG ATTTAGTAGA AAATTATAAT ACTAAAAGGC TTGAGTCATA CGGTATTAAA ATATTTTTAG GACACGCCGA GCAGAACATT ACCAATGTGT CGTATGTTGT TATTTCATCG GCAATTAATC CAAAGAATCC TGAAATAAAA GAAGCTTTGG AGCGTAAAAT TCCAATTATT AGACGTGCGG ACATGCTTGC TGAACTTATG AGATTAAAAT GTTCAGTAGC AGTTTCGGGA TCGCACGGTA AAACCACTAC TACTTCTTTA GTTGCTTGTT TATTTGAGGC AGCTGGTTTA TGTCCTACGG TTATTAACGG TGGGATCATC AATAATAAAT CGACTAATGC ATATCTTGGT TCAAGTAATT ATTTAATTGC GGAGGCCGAT GAATCGGATG CAACGTTTAT TCATATTCCA TCAACTATTG CGATAATAAC TAACATTGAT CCTGAACATT TAGATTATTA TAGAGATTTT GAAACATTAA TTGGTGCTTT TAGAAGCTTT ATTACTAATT TGCCGTTTTA CGGTTTTGCC GTTTGTTGTA TCGATCATAA AATAGTTCGT AAGTTAGTAG ACGATATTAC TGAAAGGAAG ATTGTTACTT ATGGCATCGA TTCGGAAGAT GCTCATATTA TAGCGTTTAA TATTAACACT GATATTGCCT CCTCTACTTT TGACGTAAAA ATTAGTCTAC CAAATGTACT AGGTACAACG ATTATTGAAA AAATTACTAT TCCAACGCCC GGAAGACATA ATATTTTAAA TAGTCTTGCT GCAATTGCCG TTGGGATAGA ATTAGATTTC GGTATTAAAG CTATTAAAAA CGGTTTTAAT AATTTTAAAG GAGTGAAGAG GAGATTTACT AAAGTAGCTG AATATAATAA CGCTTCAATT ATCGATGATT ACGCTCATCA CCCTGAGGAA ATCAAAGCAA CGCTTGCGAC GGCTAAAAAT ATAGCAAATA AACAAAACGG TAAAGTTATT GCTATTTTTC AGCCTCACAG ATATTCAAGA ATGCAGTATT TATTTGATGA TTTTATGTTT TGCTTTGCTG ATGCCGATAT ACTCTATATT ACTGATATAT ATGCTGCAGG TGAAAACCCT ATAGAGGGAA TTACAGGACG AAATTTAGTT GATAAGATTA CTAAGCGTAA ACATCACGAT AAAGCAAATT TTCTAGCAGA GCTAGATGAT GCGGTTGGAG TTATTATAGA TAATGCAGCT TCTGGTGATA TGATAATTAT GATGGGAGCA GGTAATATTT CAAGTTTTGC TAATGAATTA GAGGGGAGAT TGTCATCCCG TGGCTTTAGT TGTCATACCG TGGTT

Details for RiafA.00137.a.B1.GE35818

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/21/2012
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgctgtt acttgaatta aaaaaaacta atcaaacctt agaaactata cattttatag gtatcggcgg cgttggcatg agcggtattg ccgagatatt atataatcta ggttataaag tacaaggttc cgatttagta gaaaattata atactaaaag gcttgagtca tacggtatta aaatattttt aggacacgcc gagcagaaca ttaccaatgt gtcgtatgtt gttatttcat cggcaattaa tccaaagaat cctgaaataa aagaagcttt ggagcgtaaa attccaatta ttagacgtgc ggacatgctt gctgaactta tgagattaaa atgttcagta gcagtttcgg gatcgcacgg taaaaccact actacttctt tagttgcttg tttatttgag gcagctggtt tatgtcctac ggttattaac ggtgggatca tcaataataa atcgactaat gcatatcttg gttcaagtaa ttatttaatt gcggaggccg atgaatcgga tgcaacgttt attcatattc catcaactat tgcgataata actaacattg atcctgaaca tttagattat tatagagatt ttgaaacatt aattggtgct tttagaagct ttattactaa tttgccgttt tacggttttg ccgtttgttg tatcgatcat aaaatagttc gtaagttagt agacgatatt actgaaagga agattgttac ttatggcatc gattcggaag angctcatat tatagcgttt aatattaaca ctgatattgc ctcctctact tttgacgtaa aaattagtct accaaatgna ct

Expected Protein Sequence

MAHHHHHHML LLELKKTNQT LETIHFIGIG GVGMSGIAEI LYNLGYKVQG SDLVENYNTK RLESYGIKIF LGHAEQNITN VSYVVISSAI NPKNPEIKEA LERKIPIIRR ADMLAELMRL KCSVAVSGSH GKTTTTSLVA CLFEAAGLCP TVINGGIINN KSTNAYLGSS NYLIAEADES DATFIHIPST IAIITNIDPE HLDYYRDFET LIGAFRSFIT NLPFYGFAVC CIDHKIVRKL VDDITERKIV TYGIDSEDAH IIAFNINTDI ASSTFDVKIS LPNVLGTTII EKITIPTPGR HNILNSLAAI AVGIELDFGI KAIKNGFNNF KGVKRRFTKV AEYNNASIID DYAHHPEEIK ATLATAKNIA NKQNGKVIAI FQPHRYSRMQ YLFDDFMFCF ADADILYITD IYAAGENPIE GITGRNLVDK ITKRKHHDKA NFLAELDDAV GVIIDNAASG DMIIMMGAGN ISSFANELEG RLSSRGFSCH TVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt tacttgaatt aaaaaaaact aatcaaacct tagaaactat acattttata ggtatcggcg gcgttggcat gagcggtatt gccgagatat tatataatct aggttataaa gtacaaggtt ccgatttagt agaaaattat aatactaaaa ggcttgagtc atacggtatt aaaatatttt taggacacgc cgagcagaac attaccaatg tgtcgtatgt tgttatttca tcggcaatta atccaaagaa tcctgaaata aaagaagctt tggagcgtaa aattccaatt attagacgtg cggacatgct tgctgaactt atgagattaa aatgttcagt agcagtttcg ggatcgcacg gtaaaaccac tactacttct ttagttgctt gtttatttga ggcagctggt ttatgtccta cggttattaa cggtgggatc atcaataata aatcgactaa tgcatatctt ggttcaagta attatttaat tgcggaggcc gatgaatcgg atgcaacgtt tattcatatt ccatcaacta ttgcgataat aactaacatt gatcctgaac atttagatta ttatagagat tttgaaacat taattggtgc ttttagaagc tttattacta atttgccgtt ttacggtttt gccgtttgtt gtatcgatca taaaatagtt cgtaagttag tagacgatat tactgaaagg aagattgtta cttatggcat cgattcggaa gatgctcata ttatagcgtt taatattaac actgatattg cctcctctac ttttgacgta aaaattagtc taccaaatgt actaggtaca acgattattg aaaaaattac tattccaacg cccggaagac ataatatttt aaatagtctt gctgcaattg ccgttgggat agaattagat ttcggtatta aagctattaa aaacggtttt aataatttta aaggagtgaa gaggagattt actaaagtag ctgaatataa taacgcttca attatcgatg attacgctca tcaccctgag gaaatcaaag caacgcttgc gacggctaaa aatatagcaa ataaacaaaa cggtaaagtt attgctattt ttcagcctca cagatattca agaatgcagt atttatttga tgattttatg ttttgctttg ctgatgccga tatactctat attactgata tatatgctgc aggtgaaaac cctatagagg gaattacagg acgaaattta gttgataaga ttactaagcg taaacatcac gataaagcaa attttctagc agagctagat gatgcggttg gagttattat agataatgca gcttctggtg atatgataat tatgatggga gcaggtaata tttcaagttt tgctaatgaa ttagagggga gattgtcatc ccgtggcttt agttgtcata ccgtggtttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat