RiafA.00133.a

Cysteinyl-tRNA synthetase

CENTER ID: RiafA.00133.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00133.a.B1.GE35817 Full length( RiafA.00133.a ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00133.a.B1.PW36485 Full length( RiafA.00133.a ) 1 459

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.120
RefSeq: YP_002844824.1
UniProt: C3PMC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIQFHLYNT LSRTKEVFNP QDQANVKMYV CGPTVYYNPH IGNSRSGVVY DLLYRIVIKI FGEKAVKYVR NITDVDDKII DRAALLGVTI DELTDKVTKE FHKNMAYLGC MLPSIEPKAT KHIDVMIAII ERLIAKDHAY IADNHVYFDV LSAPNYTELS NRNLEEMFEG VHVENSKTKK NPQDFVLWKP AKQNESANMN FESPWGLGRP GWHIECSAMS YKYLGENFDI HGGGADLIFP HHTNEIAQSR CAFPSSTYAK YWVHNGFLTV NGEKMSKSLG NFITVRDLMD KQIQGEVVRL FLLSSHYRRP LDYNDKAIED AKKTLNYWYR AIENINVQKI DLPHDFMQSL LDDMNTPLAV KIINDYAKGV FISKTEEERQ LNASAIITCA NFIGLMNKTP NEWFNSGVDE LYVNELVNKR LEAKKQKNWL LADQIRNQLL EEKIILEDRP DGTTIWRKE
NT Sequence
ATGCAAATTC AATTTCACTT ATATAACACT CTTAGTCGTA CTAAAGAGGT TTTTAATCCT CAAGATCAAG CTAACGTAAA AATGTATGTA TGTGGACCGA CTGTTTATTA TAATCCTCAT ATAGGCAATA GTAGGTCGGG AGTAGTATAT GATTTACTTT ATCGTATAGT TATAAAAATA TTCGGTGAGA AGGCAGTAAA ATATGTACGT AACATTACGG ATGTTGACGA TAAAATTATC GATAGAGCTG CACTACTTGG TGTTACTATT GATGAGTTGA CTGATAAAGT TACGAAAGAG TTTCATAAAA ATATGGCATA TTTAGGTTGT ATGCTACCTA GTATTGAGCC GAAAGCTACA AAGCATATTG ATGTAATGAT CGCAATAATA GAACGCTTAA TAGCAAAAGA TCACGCATAT ATTGCTGATA ATCATGTTTA TTTTGATGTT TTATCAGCGC CAAATTATAC GGAATTATCT AACCGAAATT TAGAAGAAAT GTTTGAGGGA GTGCATGTAG AAAATAGTAA AACCAAAAAA AATCCACAAG ATTTTGTATT ATGGAAACCG GCAAAGCAAA ATGAATCGGC AAATATGAAT TTTGAAAGCC CCTGGGGACT TGGTCGCCCT GGATGGCATA TTGAATGTTC AGCCATGAGC TACAAATATT TAGGTGAGAA TTTTGATATT CACGGCGGCG GAGCAGATTT AATCTTTCCG CATCATACCA ATGAGATTGC TCAAAGTAGA TGTGCTTTTC CAAGTTCTAC CTATGCTAAA TATTGGGTAC ATAATGGGTT TCTAACGGTT AACGGTGAGA AAATGAGTAA ATCTCTTGGT AATTTTATTA CTGTAAGGGA TTTAATGGAT AAACAAATTC AAGGAGAAGT AGTAAGGTTA TTTTTATTAA GTAGTCACTA TAGGCGTCCT CTTGATTATA ATGATAAAGC TATAGAAGAT GCTAAGAAAA CTTTAAATTA TTGGTATAGA GCTATAGAAA ATATTAATGT GCAGAAAATA GATTTACCTC ATGATTTCAT GCAAAGCTTA CTTGATGATA TGAATACGCC GCTTGCCGTT AAAATCATTA ATGACTACGC TAAAGGTGTT TTTATTTCTA AAACTGAAGA AGAAAGGCAG CTTAACGCCT CTGCTATTAT TACTTGTGCT AATTTTATTG GTTTAATGAA TAAGACTCCA AACGAGTGGT TTAATAGCGG TGTGGATGAA CTCTATGTAA ATGAGCTTGT AAATAAGCGT TTGGAAGCAA AAAAACAAAA AAATTGGTTA TTAGCCGATC AAATTCGTAA CCAGTTATTA GAGGAAAAAA TAATTCTAGA AGATCGACCT GATGGTACTA CTATTTGGCG AAAAGAA
Details for RiafA.00133.a.B1.GE35817
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 53kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MYVCGPTVYY NPHIGNSRSG VVYDLLYRIV IKIFGEKAVK YVRNITDVDD KIIDRAALLG VTIDELTDKV TKEFHKNMAY LGCMLPSIEP KATKHIDVMI AIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVHVENSK TKKNPQDFVL WKPAKQNESA NMNFESPWGL GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPSST YAKYWVHNGF LTVNGEKMSK SLGNFITVRD LMDKQIQGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLNY WYRAIENINV QKIDLPHDFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE ERQLNASAII TCANFIGLMN KTPNEWFNSG VDELYVNELV NKRLEAKKQK NWLLADQIRN QLLEEKIILE DRPDG
Validated NT Sequence
taccatcagg tcgatcttct agaattattt tttcctctaa taactggtta cgaatttgat cggctaataa ccaatttttt tgtttttttg cttccaaacg cttatttaca agctcattta catagagttc atccacaccg ctattaaacc actcgtttgg agtcttattc attaaaccaa taaaattagc acaagtaata atagcagagg cgttaagctg cctttcttct tcagttttag aaataaaaac acctttagcg tagtcattaa tgattttaac ggcaagcggc gtattcatat catcaagtaa gctttgcatg aaatcatgag gtaaatctat tttctgcaca ttaatatttt ctatagctct ataccaataa tttaaagttt tcttagcatc ttctatagct ttatcattat aatcaagagg acgcctatag tgactactta ataaaaataa ccttactact tctccttgaa tttgtttatc cattaaatcc cttacagtaa taaaattacc aagagattta ctcattttct caccgttaac cgttagaaac ccattatgta cccaatattt agcataggta gaacttggaa aagcacatct actttgagca atctcattgg tatgatgcgg aaagattaaa tctgctccgc cgccgtgaat atcaaaattc tcacctaaat atttgtagct catggctgaa cattcaatat gccatccagg gcgaccaagt ccccaggggc tttcaaaatt catatttgcc gattcatttt gctttgccgg tttccataat acaaaatctt gtggattttt tttggtttta ctattttcta catgcactcc ctcaaacatt tcttctaaat tgcggttaga taattccgta taatttggcg ctgataaaac atcaaaataa acatgattat cagcaatata tgcgtgatct tttgctatta agcgttctat tattgcgatc attacatcaa tatgctttgt agctttcggc tcaatactag gtagcataca acctaaatat gccatatttt tatgaaactc tttcgtaact ttatcagtca actcatcaat agtaacacca agtagtgcag ctctatcgat aattttatcg tcaacatccg taatgttacg tacatatttt actgccttct caccgaatat ttttataact atacgataaa gtaaatcata tactactccc gacctactat tgcctatatg aggattataa taaacagtcg gtccacatac atacattttt acgttagctt gatcttgagg attaaaaacc tctttagtac gactaagagt gttatataag tgaaattga
Expected Protein Sequence
MAHHHHHHMQ IQFHLYNTLS RTKEVFNPQD QANVKMYVCG PTVYYNPHIG NSRSGVVYDL LYRIVIKIFG EKAVKYVRNI TDVDDKIIDR AALLGVTIDE LTDKVTKEFH KNMAYLGCML PSIEPKATKH IDVMIAIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVH VENSKTKKNP QDFVLWKPAK QNESANMNFE SPWGLGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPSSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLNYWYRAI ENINVQKIDL PHDFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEERQLN ASAIITCANF IGLMNKTPNE WFNSGVDELY VNELVNKRLE AKKQKNWLLA DQIRNQLLEE KIILEDRPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ttcaatttca cttatataac actcttagtc gtactaaaga ggtttttaat cctcaagatc aagctaacgt aaaaatgtat gtatgtggac cgactgttta ttataatcct catataggca atagtaggtc gggagtagta tatgatttac tttatcgtat agttataaaa atattcggtg agaaggcagt aaaatatgta cgtaacatta cggatgttga cgataaaatt atcgatagag ctgcactact tggtgttact attgatgagt tgactgataa agttacgaaa gagtttcata aaaatatggc atatttaggt tgtatgctac ctagtattga gccgaaagct acaaagcata ttgatgtaat gatcgcaata atagaacgct taatagcaaa agatcacgca tatattgctg ataatcatgt ttattttgat gttttatcag cgccaaatta tacggaatta tctaaccgaa atttagaaga aatgtttgag ggagtgcatg tagaaaatag taaaaccaaa aaaaatccac aagattttgt attatggaaa ccggcaaagc aaaatgaatc ggcaaatatg aattttgaaa gcccctgggg acttggtcgc cctggatggc atattgaatg ttcagccatg agctacaaat atttaggtga gaattttgat attcacggcg gcggagcaga tttaatcttt ccgcatcata ccaatgagat tgctcaaagt agatgtgctt ttccaagttc tacctatgct aaatattggg tacataatgg gtttctaacg gttaacggtg agaaaatgag taaatctctt ggtaatttta ttactgtaag ggatttaatg gataaacaaa ttcaaggaga agtagtaagg ttatttttat taagtagtca ctataggcgt cctcttgatt ataatgataa agctatagaa gatgctaaga aaactttaaa ttattggtat agagctatag aaaatattaa tgtgcagaaa atagatttac ctcatgattt catgcaaagc ttacttgatg atatgaatac gccgcttgcc gttaaaatca ttaatgacta cgctaaaggt gtttttattt ctaaaactga agaagaaagg cagcttaacg cctctgctat tattacttgt gctaatttta ttggtttaat gaataagact ccaaacgagt ggtttaatag cggtgtggat gaactctatg taaatgagct tgtaaataag cgtttggaag caaaaaaaca aaaaaattgg ttattagccg atcaaattcg taaccagtta ttagaggaaa aaataattct agaagatcga cctgatggta ctactatttg gcgaaaagaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RiafA.00133.a.B1.PW36485
PURIFICATION DATe: 9/26/2012
CONCENTRATION: 33.6mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MYVCGPTVYY NPHIGNSRSG VVYDLLYRIV IKIFGEKAVK YVRNITDVDD KIIDRAALLG VTIDELTDKV TKEFHKNMAY LGCMLPSIEP KATKHIDVMI AIIERLIAKD HAYIADNHVY FDVLSAPNYT ELSNRNLEEM FEGVHVENSK TKKNPQDFVL WKPAKQNESA NMNFESPWGL GRPGWHIECS AMSYKYLGEN FDIHGGGADL IFPHHTNEIA QSRCAFPSST YAKYWVHNGF LTVNGEKMSK SLGNFITVRD LMDKQIQGEV VRLFLLSSHY RRPLDYNDKA IEDAKKTLNY WYRAIENINV QKIDLPHDFM QSLLDDMNTP LAVKIINDYA KGVFISKTEE ERQLNASAII TCANFIGLMN KTPNEWFNSG VDELYVNELV NKRLEAKKQK NWLLADQIRN QLLEEKIILE DRPDG
Validated NT Sequence
taccatcagg tcgatcttct agaattattt tttcctctaa taactggtta cgaatttgat cggctaataa ccaatttttt tgtttttttg cttccaaacg cttatttaca agctcattta catagagttc atccacaccg ctattaaacc actcgtttgg agtcttattc attaaaccaa taaaattagc acaagtaata atagcagagg cgttaagctg cctttcttct tcagttttag aaataaaaac acctttagcg tagtcattaa tgattttaac ggcaagcggc gtattcatat catcaagtaa gctttgcatg aaatcatgag gtaaatctat tttctgcaca ttaatatttt ctatagctct ataccaataa tttaaagttt tcttagcatc ttctatagct ttatcattat aatcaagagg acgcctatag tgactactta ataaaaataa ccttactact tctccttgaa tttgtttatc cattaaatcc cttacagtaa taaaattacc aagagattta ctcattttct caccgttaac cgttagaaac ccattatgta cccaatattt agcataggta gaacttggaa aagcacatct actttgagca atctcattgg tatgatgcgg aaagattaaa tctgctccgc cgccgtgaat atcaaaattc tcacctaaat atttgtagct catggctgaa cattcaatat gccatccagg gcgaccaagt ccccaggggc tttcaaaatt catatttgcc gattcatttt gctttgccgg tttccataat acaaaatctt gtggattttt tttggtttta ctattttcta catgcactcc ctcaaacatt tcttctaaat tgcggttaga taattccgta taatttggcg ctgataaaac atcaaaataa acatgattat cagcaatata tgcgtgatct tttgctatta agcgttctat tattgcgatc attacatcaa tatgctttgt agctttcggc tcaatactag gtagcataca acctaaatat gccatatttt tatgaaactc tttcgtaact ttatcagtca actcatcaat agtaacacca agtagtgcag ctctatcgat aattttatcg tcaacatccg taatgttacg tacatatttt actgccttct caccgaatat ttttataact atacgataaa gtaaatcata tactactccc gacctactat tgcctatatg aggattataa taaacagtcg gtccacatac atacattttt acgttagctt gatcttgagg attaaaaacc tctttagtac gactaagagt gttatataag tgaaattga
Expressed Protein Sequence
MAHHHHHHMQ IQFHLYNTLS RTKEVFNPQD QANVKMYVCG PTVYYNPHIG NSRSGVVYDL LYRIVIKIFG EKAVKYVRNI TDVDDKIIDR AALLGVTIDE LTDKVTKEFH KNMAYLGCML PSIEPKATKH IDVMIAIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEEMFEGVH VENSKTKKNP QDFVLWKPAK QNESANMNFE SPWGLGRPGW HIECSAMSYK YLGENFDIHG GGADLIFPHH TNEIAQSRCA FPSSTYAKYW VHNGFLTVNG EKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL LSSHYRRPLD YNDKAIEDAK KTLNYWYRAI ENINVQKIDL PHDFMQSLLD DMNTPLAVKI INDYAKGVFI SKTEEERQLN ASAIITCANF IGLMNKTPNE WFNSGVDELY VNELVNKRLE AKKQKNWLLA DQIRNQLLEE KIILEDRPDG TTIWRKE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TTCAATTTCA CTTATATAAC ACTCTTAGTC GTACTAAAGA GGTTTTTAAT CCTCAAGATC AAGCTAACGT AAAAATGTAT GTATGTGGAC CGACTGTTTA TTATAATCCT CATATAGGCA ATAGTAGGTC GGGAGTAGTA TATGATTTAC TTTATCGTAT AGTTATAAAA ATATTCGGTG AGAAGGCAGT AAAATATGTA CGTAACATTA CGGATGTTGA CGATAAAATT ATCGATAGAG CTGCACTACT TGGTGTTACT ATTGATGAGT TGACTGATAA AGTTACGAAA GAGTTTCATA AAAATATGGC ATATTTAGGT TGTATGCTAC CTAGTATTGA GCCGAAAGCT ACAAAGCATA TTGATGTAAT GATCGCAATA ATAGAACGCT TAATAGCAAA AGATCACGCA TATATTGCTG ATAATCATGT TTATTTTGAT GTTTTATCAG CGCCAAATTA TACGGAATTA TCTAACCGAA ATTTAGAAGA AATGTTTGAG GGAGTGCATG TAGAAAATAG TAAAACCAAA AAAAATCCAC AAGATTTTGT ATTATGGAAA CCGGCAAAGC AAAATGAATC GGCAAATATG AATTTTGAAA GCCCCTGGGG ACTTGGTCGC CCTGGATGGC ATATTGAATG TTCAGCCATG AGCTACAAAT ATTTAGGTGA GAATTTTGAT ATTCACGGCG GCGGAGCAGA TTTAATCTTT CCGCATCATA CCAATGAGAT TGCTCAAAGT AGATGTGCTT TTCCAAGTTC TACCTATGCT AAATATTGGG TACATAATGG GTTTCTAACG GTTAACGGTG AGAAAATGAG TAAATCTCTT GGTAATTTTA TTACTGTAAG GGATTTAATG GATAAACAAA TTCAAGGAGA AGTAGTAAGG TTATTTTTAT TAAGTAGTCA CTATAGGCGT CCTCTTGATT ATAATGATAA AGCTATAGAA GATGCTAAGA AAACTTTAAA TTATTGGTAT AGAGCTATAG AAAATATTAA TGTGCAGAAA ATAGATTTAC CTCATGATTT CATGCAAAGC TTACTTGATG ATATGAATAC GCCGCTTGCC GTTAAAATCA TTAATGACTA CGCTAAAGGT GTTTTTATTT CTAAAACTGA AGAAGAAAGG CAGCTTAACG CCTCTGCTAT TATTACTTGT GCTAATTTTA TTGGTTTAAT GAATAAGACT CCAAACGAGT GGTTTAATAG CGGTGTGGAT GAACTCTATG TAAATGAGCT TGTAAATAAG CGTTTGGAAG CAAAAAAACA AAAAAATTGG TTATTAGCCG ATCAAATTCG TAACCAGTTA TTAGAGGAAA AAATAATTCT AGAAGATCGA CCTGATGGTA CTACTATTTG GCGAAAAGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT