RiafA.00113.a

3-oxoacyl-[acyl-carrier-protein] synthase 2

CENTER ID: RiafA.00113.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.00113.a.B1.GE35816 Full length( RiafA.00113.a ) 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.1333
RefSeq: YP_002845626.1
UniProt: C3PLP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNQRVNKRV VITGLGLVTP VGLNVSSSWK NIVDGVSGIK TITEFDTSKL ACKIAGLIDN SEKDGFKLEN FIQADDINRL SKMDKFIHYG VAAATEAVED SGWLPDDEQS RDRTGLILGS GIGGLKMIED TSIKLYQENN GKVSPFFIPA SLINLLSGLV SIKYGFSGPN QTAVTACSTG AHAIGDAMRM IKHGYADVMI AGGAEAPVTP VGVAGFVAAR ALCTKYNDNP KKASRPWDKD RSGFVMGEGA GVVVLEEYEH ALNRGAKVYG EVIGYGSTGD AYHMTAPHPE GRGAYRAMRD AILDATITPD MIDYINAHGT STTLGDGIEL AAVQKLFLEA NPKVLMSSTK SSIGHLLGAA GSVEFIFSAL AIRDQIAPPT LNLDTPMDEV NIDLVALKAK KTKIDYVLSN SFGFGGTNAS LVIKSILV
NT Sequence
ATGTCGAATC AAAGAGTAAA TAAAAGAGTA GTTATTACCG GACTTGGACT TGTTACTCCT GTTGGGCTTA ATGTTAGCTC ATCTTGGAAA AATATTGTAG ATGGAGTAAG CGGTATAAAA ACTATTACAG AATTTGACAC TTCTAAACTT GCGTGTAAAA TTGCCGGACT TATAGATAAC TCTGAGAAAG ATGGATTTAA ACTTGAAAAT TTCATACAAG CAGATGACAT TAATAGACTA AGTAAAATGG ATAAATTTAT CCATTACGGA GTAGCAGCCG CAACCGAAGC AGTTGAAGAT AGCGGCTGGT TACCCGACGA TGAACAATCT CGTGATAGAA CCGGCTTGAT ATTAGGTTCA GGAATCGGTG GGCTTAAAAT GATCGAAGAT ACCTCTATCA AACTTTATCA AGAAAATAAC GGCAAAGTTA GTCCTTTCTT TATCCCCGCT TCATTAATTA ATCTTTTATC CGGTCTTGTT TCTATAAAAT ACGGTTTTAG CGGTCCAAAC CAAACAGCAG TAACAGCTTG CTCTACAGGG GCACATGCTA TAGGCGATGC TATGCGTATG ATAAAGCACG GCTATGCCGA TGTTATGATC GCAGGCGGTG CGGAAGCTCC AGTTACACCT GTCGGAGTTG CAGGTTTCGT TGCTGCTAGA GCATTATGTA CTAAATATAA CGATAATCCT AAAAAAGCCT CAAGACCTTG GGATAAAGAC CGCAGCGGCT TTGTTATGGG TGAAGGAGCA GGCGTTGTAG TCTTAGAAGA ATATGAGCAT GCCTTAAATC GAGGGGCTAA AGTTTACGGT GAAGTTATAG GATACGGCTC TACAGGTGAC GCCTATCATA TGACAGCTCC ACATCCTGAA GGTAGAGGAG CTTATAGAGC GATGAGAGAC GCAATACTAG ATGCAACTAT CACGCCCGAT ATGATTGATT ATATTAATGC ACATGGTACT TCTACTACAC TTGGTGATGG AATAGAATTA GCAGCCGTAC AAAAATTATT TTTAGAAGCG AATCCCAAAG TTTTAATGTC TTCTACTAAA TCATCAATAG GACATTTGCT TGGTGCGGCA GGAAGTGTTG AGTTTATCTT TTCAGCTCTT GCAATTAGAG ACCAGATTGC ACCGCCAACC TTAAACTTAG ATACCCCTAT GGATGAGGTG AATATAGATT TAGTTGCACT TAAAGCTAAG AAAACTAAGA TAGATTACGT GCTTTCCAAC TCGTTCGGAT TTGGTGGTAC TAATGCTAGT TTAGTGATTA AGAGTATACT CGTT
Details for RiafA.00113.a.B1.GE35816
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 75
Validated AA Sequence
MDKFIHYGVA AATEAVEDSG WLPDDEQSRD RTGLILGSGI GGLKMIEDTS IKLYQENNGK VSPFFIPASL INLLSGLVSI KYGFSGPNQT AVTACSTGAH AIGDAMRMIK HGYADVMIAG GAEAPVTPVG VAGFVAARAL CTKYNDNPKK ASRPWDKDRS GFVMGEGAGV VVLEEYEHAL NRGAKVYGEV IGYGSTGDAY HMTAPHPEGR GAYRAMRDAI LDATITPDMI DYINAHGTST TLGDGIELAA VQKLFLEANP KVLMSSTKSS IGHLLGAAGS VEFIFSALAI RDQIAPPTLN LDTPMDEVNI DLVALKAKKT KIDYVLSNSF G
Validated NT Sequence
atccgaacga gttggaaagc acgtaatcta tcttagtttt cttagcttta agtgcaacta aatctatatt cacctcatcc ataggggtat ctaagtttaa ggttggcggt gcaatctggt ctctaattgc aagagctgaa aagataaact caacacttcc tgccgcacca agcaaatgtc ctattgatga tttagtagaa gacattaaaa ctttgggatt cgcttctaaa aataattttt gtacggctgc taattctatt ccatcaccaa gtgtagtaga agtaccatgt gcattaatat aatcaatcat atcgggcgtg atagttgcat ctagtattgc gtctctcatc gctctataag ctcctctacc ttcaggatgt ggagctgtca tatgataggc gtcacctgta gagccgtatc ctataacttc accgtaaact ttagcccctc gatttaaggc atgctcatat tcttctaaga ctacaacgcc tgctccttca cccataacaa agccgctgcg gtctttatcc caaggtcttg aggctttttt aggattatcg ttatatttag tacataatgc tctagcagca acgaaacctg caactccgac aggtgtaact ggagcttccg caccgcctgc gatcataaca tcggcatagc cgtgctttat catacgcata gcatcgccta tagcatgtgc ccctgtagag caagctgtta ctgctgtttg gtttggaccg ctaaaaccgt attttataga aacaagaccg gataaaagat taattaatga agcggggata aagaaaggac taactttgcc gttattttct tgataaagtt tgatagaggt atcttcgatc attttaagcc caccgattcc tgaacctaat atcaagccgg ttctatcacg agattgttca tcgtcgggta accagccgct atcttcaact gcttcggttg cggctgctac tccgtaatgg ataaatttat ccattttact tagtctatta atgtcatctg cttgtatgaa attttcaagt ttaaatccat ctttctcaga gttatctata agtccggcaa ttttacacgc aagtttagaa gtgtcaaatt ctgtaatagt ttttataccg cttactccat ctacaatatt tttccaagat gagctaacat taagcccaac aggagtaaca agtccaagtc cggtaataac tactctttta tttactcttt gat
Expected Protein Sequence
MAHHHHHHMS NQRVNKRVVI TGLGLVTPVG LNVSSSWKNI VDGVSGIKTI TEFDTSKLAC KIAGLIDNSE KDGFKLENFI QADDINRLSK MDKFIHYGVA AATEAVEDSG WLPDDEQSRD RTGLILGSGI GGLKMIEDTS IKLYQENNGK VSPFFIPASL INLLSGLVSI KYGFSGPNQT AVTACSTGAH AIGDAMRMIK HGYADVMIAG GAEAPVTPVG VAGFVAARAL CTKYNDNPKK ASRPWDKDRS GFVMGEGAGV VVLEEYEHAL NRGAKVYGEV IGYGSTGDAY HMTAPHPEGR GAYRAMRDAI LDATITPDMI DYINAHGTST TLGDGIELAA VQKLFLEANP KVLMSSTKSS IGHLLGAAGS VEFIFSALAI RDQIAPPTLN LDTPMDEVNI DLVALKAKKT KIDYVLSNSF GFGGTNASLV IKSILV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga atcaaagagt aaataaaaga gtagttatta ccggacttgg acttgttact cctgttgggc ttaatgttag ctcatcttgg aaaaatattg tagatggagt aagcggtata aaaactatta cagaatttga cacttctaaa cttgcgtgta aaattgccgg acttatagat aactctgaga aagatggatt taaacttgaa aatttcatac aagcagatga cattaataga ctaagtaaaa tggataaatt tatccattac ggagtagcag ccgcaaccga agcagttgaa gatagcggct ggttacccga cgatgaacaa tctcgtgata gaaccggctt gatattaggt tcaggaatcg gtgggcttaa aatgatcgaa gatacctcta tcaaacttta tcaagaaaat aacggcaaag ttagtccttt ctttatcccc gcttcattaa ttaatctttt atccggtctt gtttctataa aatacggttt tagcggtcca aaccaaacag cagtaacagc ttgctctaca ggggcacatg ctataggcga tgctatgcgt atgataaagc acggctatgc cgatgttatg atcgcaggcg gtgcggaagc tccagttaca cctgtcggag ttgcaggttt cgttgctgct agagcattat gtactaaata taacgataat cctaaaaaag cctcaagacc ttgggataaa gaccgcagcg gctttgttat gggtgaagga gcaggcgttg tagtcttaga agaatatgag catgccttaa atcgaggggc taaagtttac ggtgaagtta taggatacgg ctctacaggt gacgcctatc atatgacagc tccacatcct gaaggtagag gagcttatag agcgatgaga gacgcaatac tagatgcaac tatcacgccc gatatgattg attatattaa tgcacatggt acttctacta cacttggtga tggaatagaa ttagcagccg tacaaaaatt atttttagaa gcgaatccca aagttttaat gtcttctact aaatcatcaa taggacattt gcttggtgcg gcaggaagtg ttgagtttat cttttcagct cttgcaatta gagaccagat tgcaccgcca accttaaact tagatacccc tatggatgag gtgaatatag atttagttgc acttaaagct aagaaaacta agatagatta cgtgctttcc aactcgttcg gatttggtgg tactaatgct agtttagtga ttaagagtat actcgtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at