RiafA.00081.a
NifS-like protein
| CENTER ID: | RiafA.00081.a |
| ORGANISM: | Rickettsia africae ESF-5 |
| ASSOCIATED DISEASE: | African tick-bite fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiafA.00081.a.B1.GE35814 | Full length( RiafA.00081.a ) | 1 | 372 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiafA.00081.a.B1.PW36482 | Full length( RiafA.00081.a ) | 1 | 372 |
Structures
4IXO
DEPOSITED: 1/27/2013
DETERMINATION: XRay
CLONE: RiafA.00081.a.B1.GE35814
PROTEIN: RiafA.00081.a.B1.PW36482
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_168746 |
| PATRIC ID: | fig|347255.3.peg.818 |
| RefSeq: | YP_002845310.1 |
| UniProt: | C3PNQ7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYLDHNATT FIDPRVKEFI ISLMDKELNP SSAHSSGRFA KNLIETVRAQ IATALGITLS SREYDITFTS SGTEGNNLIM KNFYDGDIFI SAIEHLSIYN HINYAPNIKV ISVNTQGLVD LEHLEELLAQ SNTSKKLVSI IMANNESGVL QDIAEIGKIT KKYEAKFHSD LVQGFGRIPI NIKALGLDFA TISGHKIGGG QGGAALISSS NFQITPMIIG GGQEKSVRSG TENVLAIAGF GLASALRTDN ISENYIKIKK LQENLEKKLK KYPNVNIVSN NVARLPNTTL ITIPNTDAQA KLIGFDLHNI CVSSGSACSS GKISKSHVLT NMGVGEEEAK SSIRISLSHT NTVRDIEAFI EAFEEIYDVI PS
NT Sequence
ATGATATATT TAGACCATAA TGCTACTACT TTTATCGACC CTAGAGTTAA GGAATTCATA ATAAGTTTAA TGGACAAGGA GCTTAACCCT TCATCAGCAC ATAGCTCAGG TAGATTTGCT AAGAATCTCA TAGAAACGGT ACGCGCACAA ATAGCGACGG CTCTTGGTAT AACCTTATCA TCTAGAGAAT ATGATATTAC TTTTACTTCA TCAGGAACTG AAGGTAATAA TTTAATAATG AAAAATTTTT ATGACGGCGA TATTTTTATT TCAGCTATTG AACATTTATC GATCTATAAC CATATAAACT ACGCTCCAAA TATTAAAGTT ATAAGTGTTA ATACGCAAGG GCTAGTTGAT TTAGAACATT TAGAAGAGTT GTTAGCTCAA AGTAATACTA GTAAAAAACT AGTTTCCATA ATAATGGCTA ATAATGAAAG CGGAGTTTTA CAAGATATAG CTGAGATAGG TAAAATAACT AAAAAATATG AGGCAAAATT TCATAGCGAT TTAGTGCAAG GTTTTGGCAG AATACCAATA AATATCAAAG CATTAGGATT AGATTTTGCT ACAATTTCAG GGCATAAAAT AGGAGGGGGG CAGGGTGGTG CTGCTTTAAT TTCTAGCTCT AATTTTCAAA TTACTCCAAT GATTATAGGA GGAGGGCAGG AAAAATCAGT ACGCTCAGGC ACTGAAAATG TTTTAGCAAT TGCAGGATTT GGTTTAGCTT CTGCATTGAG GACAGATAAT ATCTCAGAAA ATTATATAAA AATCAAAAAG TTACAAGAAA ATTTAGAGAA AAAATTAAAA AAATATCCTA ATGTAAATAT TGTTAGTAAT AACGTAGCTA GGTTGCCTAA TACTACCTTA ATTACTATAC CAAATACGGA TGCACAAGCG AAATTAATCG GGTTTGATTT ACATAATATT TGCGTAAGTT CCGGTTCTGC TTGTTCATCG GGAAAAATAT CTAAATCGCA TGTATTAACC AATATGGGTG TAGGGGAAGA AGAAGCAAAA TCTTCGATTA GGATATCTTT AAGCCATACT AATACGGTAC GTGATATAGA GGCTTTTATA GAGGCTTTTG AGGAGATTTA CGATGTCATT CCTAGC
Details for RiafA.00081.a.B1.GE35814
| HARVESTED ON: | 8/14/2012 |
| SEQUENCED ON: | 8/18/2012 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 90 |
Validated AA Sequence
MDKELNPSSA HSSGRFAKNL IETVRAQIAT ALGITLSSRE YDITFTSSGT EGNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVISV NTQGLVDLEH LEELLAQSNT SKKLVSIIMA NNESGVLQDI AEIGKITKKY EAKFHSDLVQ GFGRIPINIK ALGLDFATIS GHKIGGGQGG AALISSSNFQ ITPMIIGGGQ EKSVRSGTEN VLAIAGFGLA SALRTDNISE NYIKIKKLQE NLEKKLKKYP NVNIVSNNVA RLPNTTLITI PNTDAQAKLI GFDLHNICVS SGSACSSGKI SKSHVLTNMG VGEEEAKSSI RISLSHTNTV RDIEAFIEAF EE
Validated NT Sequence
aatctcctca aaagcctcta taaaagcctc tatatcacgt accgtattag tatggcttaa agatatccta atcgaagatt ttgcttcttc ttcccctaca cccatattgg ttaatacatg cgatttagat atttttcccg atgaacaagc agaaccggaa cttacgcaaa tattatgtaa atcaaacccg attaatttcg cttgtgcatc cgtatttggt atagtaatta aggtagtatt aggcaaccta gctacgttat tactaacaat atttacatta ggatattttt ttaatttttt ctctaaattt tcttgtaact ttttgatttt tatataattt tctgagatat tatctgtcct caatgcagaa gctaaaccaa atcctgcaat tgctaaaaca ttttcagtgc ctgagcgtac tgatttttcc tgccctcctc ctataatcat tggagtaatt tgaaaattag agctagaaat taaagcagca ccaccctgcc cccctcctat tttatgccct gaaattgtag caaaatctaa tcctaatgct ttgatattta ttggtattct gccaaaacct tgcactaaat cgctatgaaa ttttgcctca tattttttag ttattttacc tatctcagct atatcttgta aaactccgct ttcattatta gccattatta tggaaactag ttttttacta gtattacttt gagctaacaa ctcttctaaa tgttctaaat caactagccc ttgcgtatta acacttataa ctttaatatt tggagcgtag tttatatggt tatagatcga taaatgttca atagctgaaa taaaaatatc gccgtcataa aaatttttca ttattaaatt attaccttca gttcctgatg aagtaaaagt aatatcatat tctctagatg ataaggttat accaagagcc gtcgctattt gtgcgcgtac cgtttctatg agattcttag caaatctacc tgagctatgt gctgatgaag ggttaagctc cttgtccatt aaacttatta tgaattcctt aactctaggg tcgataaaag tagtagcatt atggtc
Expected Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEFIIS LMDKELNPSS AHSSGRFAKN LIETVRAQIA TALGITLSSR EYDITFTSSG TEGNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIS VNTQGLVDLE HLEELLAQSN TSKKLVSIIM ANNESGVLQD IAEIGKITKK YEAKFHSDLV QGFGRIPINI KALGLDFATI SGHKIGGGQG GAALISSSNF QITPMIIGGG QEKSVRSGTE NVLAIAGFGL ASALRTDNIS ENYIKIKKLQ ENLEKKLKKY PNVNIVSNNV ARLPNTTLIT IPNTDAQAKL IGFDLHNICV SSGSACSSGK ISKSHVLTNM GVGEEEAKSS IRISLSHTNT VRDIEAFIEA FEEIYDVIPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat atttagacca taatgctact acttttatcg accctagagt taaggaattc ataataagtt taatggacaa ggagcttaac ccttcatcag cacatagctc aggtagattt gctaagaatc tcatagaaac ggtacgcgca caaatagcga cggctcttgg tataacctta tcatctagag aatatgatat tacttttact tcatcaggaa ctgaaggtaa taatttaata atgaaaaatt tttatgacgg cgatattttt atttcagcta ttgaacattt atcgatctat aaccatataa actacgctcc aaatattaaa gttataagtg ttaatacgca agggctagtt gatttagaac atttagaaga gttgttagct caaagtaata ctagtaaaaa actagtttcc ataataatgg ctaataatga aagcggagtt ttacaagata tagctgagat aggtaaaata actaaaaaat atgaggcaaa atttcatagc gatttagtgc aaggttttgg cagaatacca ataaatatca aagcattagg attagatttt gctacaattt cagggcataa aataggaggg gggcagggtg gtgctgcttt aatttctagc tctaattttc aaattactcc aatgattata ggaggagggc aggaaaaatc agtacgctca ggcactgaaa atgttttagc aattgcagga tttggtttag cttctgcatt gaggacagat aatatctcag aaaattatat aaaaatcaaa aagttacaag aaaatttaga gaaaaaatta aaaaaatatc ctaatgtaaa tattgttagt aataacgtag ctaggttgcc taatactacc ttaattacta taccaaatac ggatgcacaa gcgaaattaa tcgggtttga tttacataat atttgcgtaa gttccggttc tgcttgttca tcgggaaaaa tatctaaatc gcatgtatta accaatatgg gtgtagggga agaagaagca aaatcttcga ttaggatatc tttaagccat actaatacgg tacgtgatat agaggctttt atagaggctt ttgaggagat ttacgatgtc attcctagct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for RiafA.00081.a.B1.PW36482
| PURIFICATION DATe: | 9/26/2012 |
| CONCENTRATION: | 40.9mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 90 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDKELNPSSA HSSGRFAKNL IETVRAQIAT ALGITLSSRE YDITFTSSGT EGNNLIMKNF YDGDIFISAI EHLSIYNHIN YAPNIKVISV NTQGLVDLEH LEELLAQSNT SKKLVSIIMA NNESGVLQDI AEIGKITKKY EAKFHSDLVQ GFGRIPINIK ALGLDFATIS GHKIGGGQGG AALISSSNFQ ITPMIIGGGQ EKSVRSGTEN VLAIAGFGLA SALRTDNISE NYIKIKKLQE NLEKKLKKYP NVNIVSNNVA RLPNTTLITI PNTDAQAKLI GFDLHNICVS SGSACSSGKI SKSHVLTNMG VGEEEAKSSI RISLSHTNTV RDIEAFIEAF EE
Validated NT Sequence
aatctcctca aaagcctcta taaaagcctc tatatcacgt accgtattag tatggcttaa agatatccta atcgaagatt ttgcttcttc ttcccctaca cccatattgg ttaatacatg cgatttagat atttttcccg atgaacaagc agaaccggaa cttacgcaaa tattatgtaa atcaaacccg attaatttcg cttgtgcatc cgtatttggt atagtaatta aggtagtatt aggcaaccta gctacgttat tactaacaat atttacatta ggatattttt ttaatttttt ctctaaattt tcttgtaact ttttgatttt tatataattt tctgagatat tatctgtcct caatgcagaa gctaaaccaa atcctgcaat tgctaaaaca ttttcagtgc ctgagcgtac tgatttttcc tgccctcctc ctataatcat tggagtaatt tgaaaattag agctagaaat taaagcagca ccaccctgcc cccctcctat tttatgccct gaaattgtag caaaatctaa tcctaatgct ttgatattta ttggtattct gccaaaacct tgcactaaat cgctatgaaa ttttgcctca tattttttag ttattttacc tatctcagct atatcttgta aaactccgct ttcattatta gccattatta tggaaactag ttttttacta gtattacttt gagctaacaa ctcttctaaa tgttctaaat caactagccc ttgcgtatta acacttataa ctttaatatt tggagcgtag tttatatggt tatagatcga taaatgttca atagctgaaa taaaaatatc gccgtcataa aaatttttca ttattaaatt attaccttca gttcctgatg aagtaaaagt aatatcatat tctctagatg ataaggttat accaagagcc gtcgctattt gtgcgcgtac cgtttctatg agattcttag caaatctacc tgagctatgt gctgatgaag ggttaagctc cttgtccatt aaacttatta tgaattcctt aactctaggg tcgataaaag tagtagcatt atggtc
Expressed Protein Sequence
MAHHHHHHMI YLDHNATTFI DPRVKEFIIS LMDKELNPSS AHSSGRFAKN LIETVRAQIA TALGITLSSR EYDITFTSSG TEGNNLIMKN FYDGDIFISA IEHLSIYNHI NYAPNIKVIS VNTQGLVDLE HLEELLAQSN TSKKLVSIIM ANNESGVLQD IAEIGKITKK YEAKFHSDLV QGFGRIPINI KALGLDFATI SGHKIGGGQG GAALISSSNF QITPMIIGGG QEKSVRSGTE NVLAIAGFGL ASALRTDNIS ENYIKIKKLQ ENLEKKLKKY PNVNIVSNNV ARLPNTTLIT IPNTDAQAKL IGFDLHNICV SSGSACSSGK ISKSHVLTNM GVGEEEAKSS IRISLSHTNT VRDIEAFIEA FEEIYDVIPS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAT ATTTAGACCA TAATGCTACT ACTTTTATCG ACCCTAGAGT TAAGGAATTC ATAATAAGTT TAATGGACAA GGAGCTTAAC CCTTCATCAG CACATAGCTC AGGTAGATTT GCTAAGAATC TCATAGAAAC GGTACGCGCA CAAATAGCGA CGGCTCTTGG TATAACCTTA TCATCTAGAG AATATGATAT TACTTTTACT TCATCAGGAA CTGAAGGTAA TAATTTAATA ATGAAAAATT TTTATGACGG CGATATTTTT ATTTCAGCTA TTGAACATTT ATCGATCTAT AACCATATAA ACTACGCTCC AAATATTAAA GTTATAAGTG TTAATACGCA AGGGCTAGTT GATTTAGAAC ATTTAGAAGA GTTGTTAGCT CAAAGTAATA CTAGTAAAAA ACTAGTTTCC ATAATAATGG CTAATAATGA AAGCGGAGTT TTACAAGATA TAGCTGAGAT AGGTAAAATA ACTAAAAAAT ATGAGGCAAA ATTTCATAGC GATTTAGTGC AAGGTTTTGG CAGAATACCA ATAAATATCA AAGCATTAGG ATTAGATTTT GCTACAATTT CAGGGCATAA AATAGGAGGG GGGCAGGGTG GTGCTGCTTT AATTTCTAGC TCTAATTTTC AAATTACTCC AATGATTATA GGAGGAGGGC AGGAAAAATC AGTACGCTCA GGCACTGAAA ATGTTTTAGC AATTGCAGGA TTTGGTTTAG CTTCTGCATT GAGGACAGAT AATATCTCAG AAAATTATAT AAAAATCAAA AAGTTACAAG AAAATTTAGA GAAAAAATTA AAAAAATATC CTAATGTAAA TATTGTTAGT AATAACGTAG CTAGGTTGCC TAATACTACC TTAATTACTA TACCAAATAC GGATGCACAA GCGAAATTAA TCGGGTTTGA TTTACATAAT ATTTGCGTAA GTTCCGGTTC TGCTTGTTCA TCGGGAAAAA TATCTAAATC GCATGTATTA ACCAATATGG GTGTAGGGGA AGAAGAAGCA AAATCTTCGA TTAGGATATC TTTAAGCCAT ACTAATACGG TACGTGATAT AGAGGCTTTT ATAGAGGCTT TTGAGGAGAT TTACGATGTC ATTCCTAGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT