RhbaA.17251.a

L protein

CENTER ID:	RhbaA.17251.a
ORGANISM:	unclassified Rhabdoviridae Bas-Congo virus
ASSOCIATED DISEASE:	hemorrhagic fever
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	4
SELECT AGENT:	True
NIH PRIORITY pathogens category:	III

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
RhbaA.17251.a.EZ13.GE00443	Full length( RhbaA.17251.a )	1527	1950
RhbaA.17251.a.EZ17.GE00447	Full length( RhbaA.17251.a )	1651	1959
RhbaA.17251.a.FN11.GE00448	Full length( RhbaA.17251.a )	204	1058
RhbaA.17251.a.FN12.GE00449	Full length( RhbaA.17251.a )	217	1058

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
GenBank:	AFS65335.1
UniProt:	K0A117
ViPR:	link

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSLVNEYDD SHYYSENYDV FDEDEVVFND VQSEIENINT KDYNLNSPLI FNKLEGFYSK LLNGHCNPLF SHKDHDRLLE FLKLTREPLR IYQGTKDSTQ KIIASILRNY PVTKRGERFI SQWLATAEKT QTFPDSFLQG WLGDTPYDFS LSVQKIRNMK YNHRRYMSLF MDFFLIVNLI NSRSNRERDN LVKLLGGTLH YIGDLYLGCQ TVFPILGRVL IMTDFIFCID SGDLLDQNSL LLIKDTAVGR YNTFCFLNMA QIENSFDEEE KDRLELLYQL GDEMIISAGN QAFEGIKLIE AICNEAICLY TEKFRPEFPK FRSFSDHIRG EIQEKVKNGL NNIEQIHFLI KGEPNLNMVI VYYSVFRHWG HPFIDIEEGL EKLHSLVTTP ISVNEDYVNA LASDFAFKVL RSKYREKKVW YIDVTLMDKN SVLFDYVKNN AWPSQAIINK FGDNWHTLPL LPCYDLPEMV DIAAIYSDKS HSKTYSEVAQ EIQMNPNRPI PTCRVLQTLI EEPAENWPLF LKEINDNGLS KEDLIIGLKP KEREEKRIGR YFALMSWKLR QYFVYTEHII KTHFVPLVKG LTMADDLHTL IEKMLDSTCG QGSDSYQYIS IANGIDYQKW NNLQRYESTE PIFTVMGKFI GYPKLFSRTH KFFQESFVYF PDRPDRMYVN NGTINGYPGE YYCWQGQAGG FEGLRQKGWS IISCLMIERE GRCRNTQVSC LAQGDNQIII TKYKTDSWSN LRELKTNLLN IYNNNACIIE SVRKGAANLG LIINEDETIQ SIEYLNYGKI PIIRGKIIGL PPKRWSRVTC CTNDQLPSAG NLLSSVSTAA LMVSHFCNSP LDAMYCFHLF GCMVLSLSKC HNPALRGSPS SFIKDHDYLN DPLFTGVALY LDPSLGGIGG TSSTRFMIRL FPDFVTESLT FWKLIYKGTS SNLIKSLCVI IGNPSIAAFN PSHFEKLIEN PGSLNIIRGV SSSNLLKTKI KESLNQNAER IKNKIIRDAA IYNRDKEPEL MAWLESINPL FPRFISEYAN STYYGMNNSL LGLISNSRTM RKVFKNNFGK EIDDVIIRSE IISLSSIIRI LKTAQSNPDC FNDIWECSAS HADKLREISW GRRVYGMTVP HPAEMFQHAA CFGGECKFCE LSGSNSTYIT TLAPKGVRHI SIDEPRGPYP PYLGSKTKEG TSILQPWEKE TNLPLMRRAS DLRRVISWFV DPDTNLAKSI LNNLASLTGE SWEGRIEGFK RTGSALHRFQ CSRVENGGFA ACSPTPLCWL IVTTDTLSGL DQNYDFMYQP SMIYSQLSTI CMNNCTPTVY HSHISCQGCI REIEDITLDA GWEYCPEDVS QILSSWMPPT TSSWINKERT RANVASQICK WEINTNEEKS FHIGLSIGFC FADITLGSHD IIQKSDLFPL VIQKKMEPYH FIRGILRGLV IGGALHLTQR RSLLMGPKSH TAVMGIRYFL IEEITQDPKF IHLMSNGPLN EFINMYPHKV PSSYPLTTID SGSIIRGVLK RMTKDNNFIR NPDLVKPWVF SDMQSSYLLG SFGLAVIADR IIKDGLLSKV SRTRLQELQR DYIDILNDRI HPSNLEYYLG QLLPCPSELR HASKEMPDVT SLGPSFSANH IWNFSIKSTC YRVPLRCSSN PAVGPIRNIS KHWNPSVSSL RLFQCATGAH YKIDSILNDF NILPLDCLCG GDGSGGITSL LLRKFQHSKV IFNSLLCAEG IRFLGSHPSP PSAVSSLGSL SSRCVNYNDV WRNPSDLRLS ATWAYFRRLK DIHNLKITLM IFDMEVQDLS SIIEILHLLS NNLSYILEER GSLIFKTYLS SLLSRGEDNP LYALERWFDQ VDCVTCDYSS TKTSEFYLVC QRSGLKPYPV SYPTLDFLDD LWNISFINSD LKDEYQRITK IKKMLPSLSG IPTNLVPDWR VDLSTLLVIT GLESGISSDL SDQLSTQTGE DAIGGLYAVL CLLNQSTFKV WTYSESKYIP SNSLIDKFFS AYIGIMISLS IWGEDFEGFC ALDSFINNSV LQKIILNWRH QKSLVCTSWS FHHKNHLQGL TKHINISRLS ASIGHWARVA NFIISESQMS RIPLLKNCNQ KCDNIIHRYY KKGNMLEVIK KTDTFYFTPS SML

NT Sequence

ATGGACAGTC TTGTCAATGA GTACGATGAT TCCCATTACT ATAGTGAGAA TTATGATGTA TTTGATGAAG ATGAAGTGGT TTTTAATGAC GTCCAATCTG AGATTGAAAA TATTAACACA AAAGATTATA ACTTGAATTC TCCATTGATA TTTAATAAAC TAGAAGGATT TTATTCAAAA TTATTAAATG GCCACTGTAA CCCCTTATTT TCACATAAGG ATCACGACAG ATTATTAGAA TTCTTAAAGC TGACAAGGGA ACCTCTCCGC ATTTATCAGG GTACAAAGGA CTCAACTCAA AAGATTATTG CATCAATATT GAGGAACTAT CCAGTTACCA AAAGAGGTGA GAGATTTATC TCTCAATGGT TAGCAACGGC CGAAAAAACG CAGACATTCC CGGACTCATT TCTACAAGGT TGGCTTGGGG ACACCCCCTA TGATTTCTCT CTGTCAGTTC AAAAAATACG AAACATGAAA TACAATCATC GTCGATATAT GAGTTTATTT ATGGACTTTT TTCTGATTGT TAACCTCATT AATAGTAGGT CGAACAGAGA AAGAGACAAC TTAGTTAAAC TATTAGGTGG TACATTGCAT TATATAGGAG ACTTGTATTT AGGATGTCAA ACTGTCTTTC CTATTCTCGG GCGAGTTCTT ATCATGACAG ATTTCATATT TTGCATAGAT TCTGGTGACT TGTTAGATCA AAATTCTCTC TTATTAATCA AGGACACAGC CGTGGGAAGA TATAACACTT TTTGTTTCCT GAACATGGCC CAAATAGAAA ACAGTTTTGA TGAGGAAGAA AAAGACCGCC TGGAACTGCT GTATCAGCTG GGAGACGAGA TGATCATTTC TGCAGGGAAT CAGGCATTTG AAGGGATTAA ATTAATTGAG GCTATCTGTA ATGAAGCTAT CTGTCTCTAC ACAGAAAAAT TCAGACCAGA GTTTCCTAAG TTCCGTTCTT TTAGTGATCA CATCAGGGGT GAAATCCAAG AGAAAGTTAA AAACGGTCTG AATAATATAG AGCAAATTCA CTTCCTGATT AAAGGAGAAC CAAATTTAAA CATGGTCATT GTTTATTATA GTGTTTTCAG ACATTGGGGA CACCCCTTTA TTGATATAGA AGAAGGACTG GAGAAACTAC ACTCCTTGGT CACTACACCT ATTTCAGTCA ATGAAGACTA TGTTAATGCG TTGGCTAGTG ATTTTGCATT CAAAGTACTC CGTTCTAAAT ACCGGGAAAA GAAGGTGTGG TATATTGATG TAACTCTGAT GGATAAGAAC TCTGTTTTAT TTGACTATGT CAAAAATAAT GCATGGCCAT CTCAAGCCAT TATAAACAAG TTTGGTGACA ATTGGCATAC CCTCCCCTTA TTACCATGTT ATGACTTGCC AGAAATGGTT GATATAGCTG CAATATATAG TGATAAGTCA CATAGTAAAA CGTATTCAGA AGTTGCCCAA GAGATACAAA TGAATCCCAA TAGACCAATA CCTACTTGCA GAGTGCTCCA GACTCTTATT GAAGAACCAG CAGAGAATTG GCCGTTATTT CTGAAAGAAA TTAATGATAA TGGATTAAGC AAAGAAGATT TAATTATTGG TCTGAAACCA AAAGAAAGAG AAGAAAAAAG GATAGGACGA TACTTTGCTT TGATGTCTTG GAAATTAAGA CAATATTTTG TTTACACAGA GCACATTATT AAAACCCATT TTGTACCTTT GGTTAAAGGG CTCACTATGG CAGATGATTT ACATACGCTA ATAGAGAAAA TGTTAGATTC AACATGCGGG CAAGGAAGTG ACAGTTATCA ATACATAAGT ATCGCAAATG GTATCGATTA TCAAAAGTGG AACAATCTTC AAAGATATGA GTCTACCGAA CCAATTTTTA CTGTAATGGG CAAATTTATA GGCTACCCCA AACTGTTTTC ACGTACACAT AAATTCTTTC AAGAAAGTTT TGTATACTTC CCGGACAGAC CAGACAGGAT GTATGTGAAC AACGGCACCA TCAATGGGTA CCCAGGAGAA TATTATTGTT GGCAAGGTCA AGCAGGTGGT TTTGAGGGTT TGAGGCAGAA AGGATGGTCA ATTATCAGTT GTCTTATGAT AGAGAGAGAA GGGAGATGTA GGAACACCCA AGTGTCTTGT TTGGCACAAG GAGACAATCA GATCATTATT ACAAAGTATA AAACAGATTC TTGGTCAAAT CTGAGAGAAC TTAAAACTAA TCTTTTGAAC ATATATAATA ACAATGCGTG CATTATAGAA TCAGTTAGGA AAGGAGCAGC GAATTTAGGA TTAATCATTA ATGAGGATGA GACAATTCAA AGCATTGAGT ACCTAAACTA TGGCAAAATC CCCATAATTA GAGGGAAAAT CATAGGCCTC CCTCCTAAAA GATGGTCTCG AGTGACATGC TGCACTAATG ATCAACTTCC TTCAGCTGGG AATCTTCTTT CTTCGGTCTC TACTGCAGCA TTGATGGTGT CTCACTTCTG CAATAGCCCA CTCGATGCGA TGTATTGTTT CCATTTGTTT GGATGTATGG TCCTCAGTTT ATCCAAATGT CATAATCCCG CACTCAGAGG ATCACCGTCT AGTTTCATCA AAGATCATGA TTACCTCAAC GATCCTTTAT TCACAGGTGT TGCATTGTAT CTGGATCCTA GTTTGGGAGG GATAGGAGGG ACATCTTCCA CCAGATTCAT GATTAGACTC TTCCCAGACT TTGTAACTGA GAGTTTAACA TTCTGGAAAC TAATTTATAA AGGAACCTCT TCGAATTTGA TCAAATCTCT GTGTGTGATA ATCGGAAACC CTAGCATTGC AGCCTTTAAT CCATCTCATT TTGAAAAACT CATAGAGAAT CCTGGGTCGT TAAATATAAT TAGAGGTGTG AGCTCCAGCA ATCTGCTAAA GACAAAGATA AAAGAAAGCC TTAACCAAAA TGCAGAGAGA ATAAAAAATA AAATTATCAG AGATGCTGCT ATATACAACA GAGATAAAGA GCCCGAGCTA ATGGCCTGGT TAGAGAGCAT AAACCCACTA TTTCCTAGAT TTATAAGTGA GTACGCAAAT TCTACATATT ACGGAATGAA TAATTCTTTG CTAGGATTAA TTTCTAACTC AAGGACGATG AGGAAAGTTT TCAAAAACAA TTTTGGTAAG GAGATAGATG ATGTGATCAT TCGGAGTGAA ATCATTTCTC TCTCGTCTAT TATAAGAATT CTAAAAACAG CTCAGTCTAA TCCTGATTGT TTTAATGATA TCTGGGAATG TTCTGCATCG CATGCAGACA AATTAAGAGA AATCTCTTGG GGTCGTAGAG TATATGGGAT GACAGTTCCT CATCCAGCAG AGATGTTCCA GCATGCAGCC TGTTTTGGGG GAGAGTGCAA ATTTTGTGAA CTCTCTGGGT CCAATTCAAC CTATATAACC ACTCTAGCAC CAAAAGGAGT AAGGCACATA TCAATAGATG AGCCCAGAGG CCCCTATCCC CCATATTTAG GTTCCAAAAC AAAGGAAGGC ACAAGTATCC TTCAACCATG GGAAAAAGAA ACAAATCTGC CACTGATGAG AAGGGCCTCA GATTTGCGGA GAGTAATCAG TTGGTTTGTA GACCCGGACA CCAATCTGGC CAAGTCGATA TTGAATAATC TAGCTTCTCT GACAGGAGAA TCTTGGGAAG GTCGTATAGA GGGGTTTAAA AGAACCGGGT CTGCTCTTCA TCGCTTCCAA TGCTCGAGAG TTGAAAACGG GGGTTTTGCT GCATGCTCAC CGACTCCTCT ATGTTGGTTA ATTGTTACAA CAGATACGTT ATCCGGCCTT GACCAAAATT ATGATTTTAT GTATCAGCCA TCTATGATAT ACTCACAACT TAGTACAATA TGTATGAATA ATTGCACCCC TACTGTGTAC CATAGTCATA TATCATGTCA AGGCTGCATC AGAGAAATAG AAGACATCAC TTTGGATGCC GGCTGGGAGT ATTGTCCTGA AGATGTTTCT CAGATCTTAT CTAGTTGGAT GCCACCTACC ACCTCTTCAT GGATTAACAA AGAAAGAACG CGGGCAAATG TAGCAAGTCA AATCTGTAAG TGGGAAATAA ATACAAATGA AGAGAAAAGT TTCCATATTG GGCTATCTAT TGGTTTCTGT TTTGCCGATA TAACCTTAGG TTCTCATGAT ATCATCCAGA AATCTGACCT TTTCCCGTTA GTCATCCAAA AGAAAATGGA ACCTTACCAT TTCATCAGAG GTATACTTAG AGGGTTAGTG ATCGGGGGTG CCTTACATCT CACTCAAAGG AGAAGTTTGC TCATGGGGCC AAAATCCCAC ACAGCTGTAA TGGGGATCCG ATATTTTTTA ATTGAGGAAA TAACACAGGA CCCTAAATTT ATCCATTTAA TGTCAAATGG ACCTCTGAAC GAATTTATAA ACATGTACCC CCACAAGGTT CCATCATCTT ATCCTCTGAC AACTATTGAT TCAGGTTCTA TAATAAGAGG TGTTTTAAAG AGAATGACTA AAGATAATAA TTTTATCAGA AATCCAGATC TAGTTAAGCC TTGGGTATTC TCCGATATGC AGTCTTCATA TTTATTAGGA TCATTTGGTT TGGCAGTCAT AGCAGATAGG ATCATCAAAG ATGGATTATT ATCTAAAGTC TCTCGGACAC GCCTCCAGGA GTTACAACGT GACTATATTG ATATACTAAA TGACAGAATT CATCCTTCGA ATTTAGAATA TTACCTAGGT CAACTTTTGC CCTGTCCATC AGAATTAAGA CATGCAAGCA AAGAAATGCC AGATGTTACA TCATTAGGTC CAAGTTTCTC TGCTAATCAC ATATGGAATT TCTCCATTAA ATCAACTTGC TACCGGGTCC CTCTGAGATG CAGCTCTAAT CCTGCAGTGG GACCGATAAG AAACATTAGC AAACATTGGA ACCCCAGTGT ATCTAGCCTC AGATTATTTC AATGTGCAAC AGGAGCACAT TATAAGATAG ACTCTATTTT AAATGATTTT AATATTCTTC CTCTTGATTG TCTGTGTGGA GGAGATGGAT CAGGAGGTAT CACATCTTTG TTACTAAGAA AATTCCAACA TAGTAAAGTC ATCTTTAACA GTCTCCTCTG TGCCGAAGGA ATTCGATTTC TGGGATCACA TCCTTCCCCT CCATCCGCTG TAAGCTCTTT AGGGAGCCTT AGCTCACGAT GTGTGAATTA TAATGATGTT TGGAGAAATC CTAGTGATCT GAGGCTTTCT GCAACCTGGG CATATTTTAG AAGATTAAAA GATATCCACA ATTTAAAGAT AACGCTGATG ATTTTTGACA TGGAAGTCCA AGATCTTAGT TCAATCATAG AAATTTTACA TCTTTTATCA AACAATCTCT CTTATATTCT AGAGGAGAGA GGAAGTTTAA TCTTTAAGAC TTATCTATCA AGTCTATTAT CCAGAGGAGA GGACAATCCC CTCTATGCAT TAGAAAGGTG GTTTGATCAA GTAGATTGTG TAACTTGTGA TTATTCTTCA ACAAAGACAT CAGAGTTCTA CCTAGTTTGT CAAAGGTCAG GCCTCAAACC CTATCCTGTA TCCTATCCCA CTCTAGATTT CTTGGATGAT TTATGGAACA TTAGTTTCAT AAATTCAGAC CTAAAGGATG AATATCAAAG GATAACCAAA ATTAAGAAGA TGCTTCCAAG CTTATCAGGT ATCCCAACTA ATCTTGTTCC TGATTGGAGA GTCGACCTAT CTACGCTATT AGTCATTACA GGATTGGAGT CAGGGATCAG TTCTGACTTA TCAGACCAAT TATCTACCCA GACCGGAGAA GATGCCATTG GAGGGCTTTA TGCTGTATTG TGTTTATTAA ATCAGTCCAC ATTCAAAGTG TGGACATATA GTGAGAGTAA ATACATCCCA TCTAATTCGT TGATAGATAA ATTCTTTAGT GCTTATATAG GAATAATGAT TTCACTCTCA ATTTGGGGAG AAGATTTTGA GGGGTTCTGC GCCTTAGATA GTTTCATCAA TAATTCTGTC CTACAAAAGA TCATATTAAA TTGGAGACAT CAGAAATCCC TGGTTTGCAC ATCTTGGTCC TTTCACCATA AAAATCACCT CCAAGGACTC ACCAAGCATA TTAACATAAG CAGGTTATCA GCAAGTATCG GACACTGGGC CAGGGTGGCA AATTTTATTA TATCAGAGTC TCAGATGTCA AGAATTCCCC TATTAAAGAA TTGTAACCAG AAATGTGATA ACATTATTCA CCGTTACTAC AAAAAAGGGA ATATGCTAGA AGTAATAAAG AAAACAGACA CTTTCTATTT CACACCAAGT TCTATGCTAT AA

Details for RhbaA.17251.a.EZ13.GE00443

HARVESTED ON:	4/14/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG PWVFSDMQSS YLLGSFGLAV IADRIIKDGL LSKVSRTRLQ ELQRDYIDIL NDRIHPSNLE YYLGQLLPCP SELRHASKEM PDVTSLGPSF SANHIWNFSI KSTCYRVPLR CSSNPAVGPI RNISKHWNPS VSSLRLFQCA TGAHYKIDSI LNDFNILPLD CLCGGDGSGG ITSLLLRKFQ HSKVIFNSLL CAEGIRFLGS HPSPPSAVSS LGSLSSRCVN YNDVWRNPSD LRLSATWAYF RRLKDIHNLK ITLMIFDMEV QDLSSIIEIL HLLSNNLSYI LEERGSLIFK TYLSSLLSRG EDNPLYALER WFDQVDCVTC DYSSTKTSEF YLVCQRSGLK PYPVSYPTLD FLDDLWNISF INSDLKDEYQ RITKIKKMLP SLSGIPTNLV PDWRVDLSTL LVITGLESGI SSDLSDQLST QTG

Full NT Sequence (Expression Vector + Insert)

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttttgag accttaagga ggtaaaaaat gggtagcagc catcatcatc accatcacag cagcggcgaa aatctgtatt ttcagggtcc gtgggtgttt agcgatatgc aatcttctta cctgttgggt agctttggtt tggcagttat cgcggaccgc attatcaagg acggtctgct gagcaaagtc agccgtacgc gcctgcaaga actgcagcgc gactacattg atatcctgaa tgaccgtatc cacccaagca acctcgaata ctacctgggc cagttgctgc cgtgcccgag cgagctgcgt cacgcatcta aagaaatgcc agacgttacc agcctgggtc cgagcttctc cgcgaaccac atctggaact tcagcattaa gtcaacctgt tatcgtgtcc cgctgcgttg ctcaagcaat ccagccgtcg gcccgatccg caacatcagc aaacactgga acccgtcggt ttccagcctg cgtctgtttc aatgcgcaac cggtgcgcac tataagatcg acagcatctt gaatgatttc aacatcctgc cgctggactg tctgtgcggt ggcgacggta gcggtggcat tacctctctg ctgctgcgta agtttcagca cagcaaggtc attttcaata gcttactgtg cgccgagggt attcgtttcc tgggtagcca tccgagcccg ccttctgccg tcagcagcct gggctcgctt tcctcccgtt gcgtgaacta caacgacgtt tggcgtaatc cgagcgatct gcgcctgagc gcgacgtggg cttattttcg tcgcctgaaa gatattcaca acctgaagat caccttgatg atttttgata tggaagttca agacctgagc agcatcatcg agattctgca tctgctgtcg aacaatctga gctatattct ggaggagcgt ggtagcctga ttttcaagac ctacttgagc tccctgctgt cccgtggtga agataacccg ctgtacgcgt tggagcgctg gtttgatcaa gttgactgtg tgacgtgtga ctacagcagc actaaaacgt ccgagttcta tctggtgtgc cagcgcagcg gcttaaaacc gtatcctgtc agctacccga cgttggattt cctggatgat ttgtggaaca tctcgttcat caatagcgac ctgaaagatg agtaccaacg tattaccaag atcaagaaaa tgctcccgag cctgagcggc attccgacca atctggtgcc ggactggcgt gttgatttgt ctaccctgct ggtgattacg ggtctggaga gcggcatctc cagcgacctg agcgaccagc tgagcaccca gactggctaa ggttgaggtc tcacccccta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcccct gagacgcgtc aatcgagttc gtacctaagg gcgacacccc ctaattagcc cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagtc cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa ggggtgttat gagccatatt caggtataaa tgggctcgcg ataatgttca gaattggtta attggttgta acactgaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgccac ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcgttc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcactcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaagcggc gcgccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgagg acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga tagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgccagtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagac

Details for RhbaA.17251.a.EZ17.GE00447

HARVESTED ON:	4/14/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG KHWNPSVSSL RLFQCATGAH YKIDSILNDF NILPLDCLCG GDGSGGITSL LLRKFQHSKV IFNSLLCAEG IRFLGSHPSP PSAVSSLGSL SSRCVNYNDV WRNPSDLRLS ATWAYFRRLK DIHNLKITLM IFDMEVQDLS SIIEILHLLS NNLSYILEER GSLIFKTYLS SLLSRGEDNP LYALERWFDQ VDCVTCDYSS TKTSEFYLVC QRSGLKPYPV SYPTLDFLDD LWNISFINSD LKDEYQRITK IKKMLPSLSG IPTNLVPDWR VDLSTLLVIT GLESGISSDL SDQLSTQTGE DAIGGLYA

Full NT Sequence (Expression Vector + Insert)

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttttgag accttaagga ggtaaaaaat gggtagcagc catcatcatc accatcacag cagcggcgaa aatctgtatt ttcaaggtaa acactggaac ccgtcggttt ccagcctgcg tctgtttcaa tgcgcaaccg gtgcgcacta taagatcgac agcatcttga atgatttcaa catcctgccg ctggactgtc tgtgcggtgg cgacggtagc ggtggcatta cctctctgct gctgcgtaag tttcagcaca gcaaggtcat tttcaatagc ttactgtgcg ccgagggtat tcgtttcctg ggtagccatc cgagcccgcc ttctgccgtc agcagcctgg gctcgctttc ctcccgttgc gtgaactaca acgacgtttg gcgtaatccg agcgatctgc gcctgagcgc gacgtgggct tattttcgtc gcctgaaaga tattcacaac ctgaagatca ccttgatgat ttttgatatg gaagttcaag acctgagcag catcatcgag attctgcatc tgctgtcgaa caatctgagc tatattctgg aggagcgtgg tagcctgatt ttcaagacct acttgagctc cctgctgtcc cgtggtgaag ataacccgct gtacgcgttg gagcgctggt ttgatcaagt tgactgtgtg acgtgtgact acagcagcac taaaacgtcc gagttctatc tggtgtgcca gcgcagcggc ttaaaaccgt atcctgtcag ctacccgacg ttggatttcc tggatgattt gtggaacatc tcgttcatca atagcgacct gaaagatgag taccaacgta ttaccaagat caagaaaatg ctcccgagcc tgagcggcat tccgaccaat ctggtgccgg actggcgtgt tgatttgtct accctgctgg tgattacggg tctggagagc ggcatctcca gcgacctgag cgaccagctg agcacccaga ctggcgaaga tgcgattggt ggcctgtatg cttaaggttg aggtctcacc ccctagcata accccttggg gcctctaaac gggtcttgag gggttttttg cccctgagac gcgtcaatcg agttcgtacc taagggcgac accccctaat tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gccacttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cgttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcac tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaggacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagatagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc cagtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgactcat gaccaaaatc ccttaacgtg agttacgcgc gcgtcgttcc actgagcgtc agac

Details for RhbaA.17251.a.FN11.GE00448

HARVESTED ON:	4/14/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	100kDa
OBSERVED MW:	100kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG DLYLGCQTVF PILGRVLIMT DFIFCIDSGD LLDQNSLLLI KDTAVGRYNT FCFLNMAQIE NSFDEEEKDR LELLYQLGDE MIISAGNQAF EGIKLIEAIC NEAICLYTEK FRPEFPKFRS FSDHIRGEIQ EKVKNGLNNI EQIHFLIKGE PNLNMVIVYY SVFRHWGHPF IDIEEGLEKL HSLVTTPISV NEDYVNALAS DFAFKVLRSK YREKKVWYID VTLMDKNSVL FDYVKNNAWP SQAIINKFGD NWHTLPLLPC YDLPEMVDIA AIYSDKSHSK TYSEVAQEIQ MNPNRPIPTC RVLQTLIEEP AENWPLFLKE INDNGLSKED LIIGLKPKER EEKRIGRYFA LMSWKLRQYF VYTEHIIKTH FVPLVKGLTM ADDLHTLIEK MLDSTCGQGS DSYQYISIAN GIDYQKWNNL QRYESTEPIF TVMGKFIGYP KLFSRTHKFF QESFVYFPDR PDRMYVNNGT INGYPGEYYC WQGQAGGFEG LRQKGWSIIS CLMIEREGRC RNTQVSCLAQ GDNQIIITKY KTDSWSNLRE LKTNLLNIYN NNACIIESVR KGAANLGLII NEDETIQSIE YLNYGKIPII RGKIIGLPPK RWSRVTCCTN DQLPSAGNLL SSVSTAALMV SHFCNSPLDA MYCFHLFGCM VLSLSKCHNP ALRGSPSSFI KDHDYLNDPL FTGVALYLDP SLGGIGGTSS TRFMIRLFPD FVTESLTFWK LIYKGTSSNL IKSLCVIIGN PSIAAFNPSH FEKLIENPGS LNIIRGVSSS NLLKTKIKES LNQNAERIKN KIIRDAAIYN RDKEPELMAW LESINPLFPR FISEYANSTY YGMNNSLLGL ISNSRTMRKV FKNN

Full NT Sequence (Expression Vector + Insert)

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttttgag accttaagga ggtaaaaaat gggcagctca caccatcacc accaccactc ttccggcgaa aatctgtact ttcaagggga tctttacctg ggctgtcaga ctgtgtttcc aattctcggt cgcgtgctta ttatgactga cttcattttc tgtatcgatt ctggcgatct gttggatcaa aattccctgc ttctgattaa ggacaccgcc gttggccgct acaatacctt ctgtttcctt aatatggctc agatcgaaaa ctctttcgat gaggaagaga aagaccgcct cgaactgctg tatcagttgg gtgatgaaat gattatttct gccggcaatc aagccttcga gggtatcaag ctcatcgaag ccatttgtaa cgaagcaatt tgtctgtata ctgagaaatt ccggccggag ttcccaaagt ttaggagctt ttctgatcac atcagaggcg aaattcagga gaaggttaaa aacggtctga ataacatcga acagatccac ttcttgatta agggcgaacc taatctgaat atggtgatcg tgtattactc tgtatttcga cactggggcc acccctttat tgatattgaa gagggcctgg agaagctcca ctcacttgtg accaccccga ttagtgtgaa tgaggattat gtgaacgctc tggcctctga tttcgcattc aaggtcctgc gctcaaagta ccgcgagaag aaagtttggt atatcgacgt tactctgatg gataagaaca gcgtcctgtt cgactatgtg aagaacaatg cctggccaag ccaggctatc attaataagt tcggtgataa ctggcacacg ttgcctcttc tgccatgcta tgatctgcct gagatggtcg acatcgcagc catctattct gataagtccc actccaagac ctactccgaa gtggcgcaag agattcagat gaatccaaac agacctatcc caacttgccg cgtgctgcag actctaatcg aagaaccagc cgagaactgg ccacttttcc tgaaagaaat taacgataac ggcctctcaa aagaggatct gattattggc ctgaagccaa aggagagaga agagaaacgt atcggcagat acttcgcttt gatgtcttgg aagctgcgcc agtacttcgt gtataccgag cacattatta agacgcactt cgtgccactt gtcaagggcc tgactatggc cgatgatctg catacgctta ttgagaaaat gttggattcc acttgtgggc agggcagcga ctcctaccaa tacatctcaa ttgctaatgg tatcgactat cagaagtgga ataatcttca gagatacgaa agcactgaac cgattttcac tgtgatgggc aaattcatcg gttacccaaa gctgttctct cgcactcaca agttcttcca agagtcattt gtgtactttc cggatcgtcc agaccgcatg tatgtgaaca acggcactat taacggctat cctggagagt actactgttg gcagggacag gccggtggct tcgagggctt acgtcaaaag ggttggagca tcatctcttg tttgatgatc gagcgcgagg gcagatgtag aaacactcag gtgtcctgtt tggcccaggg cgataatcag attatcatca ctaagtacaa gactgattct tggagcaatc tgcgtgagct caagactaat ctgttaaaca tctataacaa taatgcctgc attatcgagt ctgtgcgcaa gggcgcggct aacctgggtc tgattatcaa cgaggatgaa acgattcaga gtattgagta tcttaattat ggaaagattc ctattattcg aggaaagatt atcggcctgc cacctaagag atggtcacgc gtcacctgtt gtaccaatga ccagttgccg agcgctggca atttgctgtc aagcgtgtca accgccgccc tgatggtgtc ccatttctgt aactcccctc tggatgccat gtattgcttc cacctgttcg gctgtatggt tctgagcttg tcgaagtgcc acaacccagc tttgaggggt tctccaagct cctttattaa ggatcatgac tacctgaacg atccactctt cactggcgta gccctgtacc ttgatccttc tctgggtggc ataggcggca cgtcctcaac ccgcttcatg attcgcctgt ttccggactt cgtgacagag agcctgacct tttggaagct aatctataag ggcacttcat ctaacctgat taagtctttg tgtgtcatta tcggcaaccc tagcattgct gcctttaatc caagccattt tgaaaagttg attgagaatc ctggtagcct gaatatcatt cgcggcgtta gcagcagtaa tctgcttaag accaagatta aggaaagcct gaatcagaat gccgagcgta ttaagaacaa aatcattcgt gatgcggcta tctataaccg cgacaaagag cctgagctga tggcatggct ggagtcaatc aacccgctgt tcccccgctt catttctgag tacgctaata gcacatacta tggcatgaac aactccctgc tgggccttat ttctaattct cgtaccatgc gcaaggtctt taagaacaat taaggttgag gtctcacccc ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgcc cctgagacgc gtcaatcgag ttcgtaccta agggcgacac cccctaatta gcccgggcga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga gtccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa caaggggtgt tatgagccat attcaggtat aaatgggctc gcgataatgt tcagaattgg ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaagc ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agatagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgcca gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgactcatga ccaaaatccc ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag ac

Details for RhbaA.17251.a.FN12.GE00449

HARVESTED ON:	4/14/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	99kDa
OBSERVED MW:	99kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGSSHHHHHH SSGENLYFQG GRVLIMTDFI FCIDSGDLLD QNSLLLIKDT AVGRYNTFCF LNMAQIENSF DEEEKDRLEL LYQLGDEMII SAGNQAFEGI KLIEAICNEA ICLYTEKFRP EFPKFRSFSD HIRGEIQEKV KNGLNNIEQI HFLIKGEPNL NMVIVYYSVF RHWGHPFIDI EEGLEKLHSL VTTPISVNED YVNALASDFA FKVLRSKYRE KKVWYIDVTL MDKNSVLFDY VKNNAWPSQA IINKFGDNWH TLPLLPCYDL PEMVDIAAIY SDKSHSKTYS EVAQEIQMNP NRPIPTCRVL QTLIEEPAEN WPLFLKEIND NGLSKEDLII GLKPKEREEK RIGRYFALMS WKLRQYFVYT EHIIKTHFVP LVKGLTMADD LHTLIEKMLD STCGQGSDSY QYISIANGID YQKWNNLQRY ESTEPIFTVM GKFIGYPKLF SRTHKFFQES FVYFPDRPDR MYVNNGTING YPGEYYCWQG QAGGFEGLRQ KGWSIISCLM IEREGRCRNT QVSCLAQGDN QIIITKYKTD SWSNLRELKT NLLNIYNNNA CIIESVRKGA ANLGLIINED ETIQSIEYLN YGKIPIIRGK IIGLPPKRWS RVTCCTNDQL PSAGNLLSSV STAALMVSHF CNSPLDAMYC FHLFGCMVLS LSKCHNPALR GSPSSFIKDH DYLNDPLFTG VALYLDPSLG GIGGTSSTRF MIRLFPDFVT ESLTFWKLIY KGTSSNLIKS LCVIIGNPSI AAFNPSHFEK LIENPGSLNI IRGVSSSNLL KTKIKESLNQ NAERIKNKII RDAAIYNRDK EPELMAWLES INPLFPRFIS EYANSTYYGM NNSLLGLISN SRTMRKVFKN N

Full NT Sequence (Expression Vector + Insert)

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttttgag accttaagga ggtaaaaaat gggcagctca caccatcacc accaccactc ttccggcgaa aatctgtact ttcaaggcgg tcgcgtgctt attatgactg acttcatttt ctgtatcgat tctggcgatc tgttggatca aaattccctg cttctgatta aggacaccgc cgttggccgc tacaatacct tctgtttcct taatatggct cagatcgaaa actctttcga tgaggaagag aaagaccgcc tcgaactgct gtatcagttg ggtgatgaaa tgattatttc tgccggcaat caagccttcg agggtatcaa gctcatcgaa gccatttgta acgaagcaat ttgtctgtat actgagaaat tccggccgga gttcccaaag tttaggagct tttctgatca catcagaggc gaaattcagg agaaggttaa aaacggtctg aataacatcg aacagatcca cttcttgatt aagggcgaac ctaatctgaa tatggtgatc gtgtattact ctgtatttcg acactggggc caccccttta ttgatattga agagggcctg gagaagctcc actcacttgt gaccaccccg attagtgtga atgaggatta tgtgaacgct ctggcctctg atttcgcatt caaggtcctg cgctcaaagt accgcgagaa gaaagtttgg tatatcgacg ttactctgat ggataagaac agcgtcctgt tcgactatgt gaagaacaat gcctggccaa gccaggctat cattaataag ttcggtgata actggcacac gttgcctctt ctgccatgct atgatctgcc tgagatggtc gacatcgcag ccatctattc tgataagtcc cactccaaga cctactccga agtggcgcaa gagattcaga tgaatccaaa cagacctatc ccaacttgcc gcgtgctgca gactctaatc gaagaaccag ccgagaactg gccacttttc ctgaaagaaa ttaacgataa cggcctctca aaagaggatc tgattattgg cctgaagcca aaggagagag aagagaaacg tatcggcaga tacttcgctt tgatgtcttg gaagctgcgc cagtacttcg tgtataccga gcacattatt aagacgcact tcgtgccact tgtcaagggc ctgactatgg ccgatgatct gcatacgctt attgagaaaa tgttggattc cacttgtggg cagggcagcg actcctacca atacatctca attgctaatg gtatcgacta tcagaagtgg aataatcttc agagatacga aagcactgaa ccgattttca ctgtgatggg caaattcatc ggttacccaa agctgttctc tcgcactcac aagttcttcc aagagtcatt tgtgtacttt ccggatcgtc cagaccgcat gtatgtgaac aacggcacta ttaacggcta tcctggagag tactactgtt ggcagggaca ggccggtggc ttcgagggct tacgtcaaaa gggttggagc atcatctctt gtttgatgat cgagcgcgag ggcagatgta gaaacactca ggtgtcctgt ttggcccagg gcgataatca gattatcatc actaagtaca agactgattc ttggagcaat ctgcgtgagc tcaagactaa tctgttaaac atctataaca ataatgcctg cattatcgag tctgtgcgca agggcgcggc taacctgggt ctgattatca acgaggatga aacgattcag agtattgagt atcttaatta tggaaagatt cctattattc gaggaaagat tatcggcctg ccacctaaga gatggtcacg cgtcacctgt tgtaccaatg accagttgcc gagcgctggc aatttgctgt caagcgtgtc aaccgccgcc ctgatggtgt cccatttctg taactcccct ctggatgcca tgtattgctt ccacctgttc ggctgtatgg ttctgagctt gtcgaagtgc cacaacccag ctttgagggg ttctccaagc tcctttatta aggatcatga ctacctgaac gatccactct tcactggcgt agccctgtac cttgatcctt ctctgggtgg cataggcggc acgtcctcaa cccgcttcat gattcgcctg tttccggact tcgtgacaga gagcctgacc ttttggaagc taatctataa gggcacttca tctaacctga ttaagtcttt gtgtgtcatt atcggcaacc ctagcattgc tgcctttaat ccaagccatt ttgaaaagtt gattgagaat cctggtagcc tgaatatcat tcgcggcgtt agcagcagta atctgcttaa gaccaagatt aaggaaagcc tgaatcagaa tgccgagcgt attaagaaca aaatcattcg tgatgcggct atctataacc gcgacaaaga gcctgagctg atggcatggc tggagtcaat caacccgctg ttcccccgct tcatttctga gtacgctaat agcacatact atggcatgaa caactccctg ctgggcctta tttctaattc tcgtaccatg cgcaaggtct ttaagaacaa ttaaggttga ggtctcaccc cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc ccctgagacg cgtcaatcga gttcgtacct aagggcgaca ccccctaatt agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg ccacttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc gttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcact cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgactcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca gac