RhbaA.17093.a

N protein

CENTER ID: RhbaA.17093.a
ORGANISM: unclassified Rhabdoviridae Bas-Congo virus
ASSOCIATED DISEASE: hemorrhagic fever
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 4
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 III

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RhbaA.17093.a.D11.PD00303 N-truncation 24 430
External Resources
RESOURCE REFERENCE ID
GenBank: AFS65337.1
UniProt: K0A1N2
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSLLSNKTI TCNLPKEDNL AQYPRTYFES NGFKKPSLRL PKSNLSLKDL REIIKGSLKA STIDPEYTLL FLKQYVTRFK AQIAEDWISF DVKIGRAGET ISPLNLFAIE EYDPKLRDMV KDTTAEEDDD HWMAFVLLSI YRISKVLNPA YRATLIGKMN SQAQGINSNA LKVLDNAVIF NVLSSNNSYV KLVAGTDMIL FRMGDGEHSL YRWGTVVTRH EDCSALLSIA HICKLLGKEI EEFLEWIFVN SVGRDVEKIL EPGQELDKPY SYGPYMKGLG LVTRSIYSAS ACSNLYTFCH MLGAVLHSDR SKHARMINEG NLINIKLNTS VIAWVLGSRG SLVQIFVKNS RKDEVLSKQT KNQGKIETDS LVSFDEDEIP QTSDPHEWYD YLARRDFDIP DVIMNAVRNE AKKLVGTRIG TIGHYVQTTY
NT Sequence
ATGTTTAGTT TGTTAAGTAA TAAGACTATA ACTTGTAATC TTCCTAAGGA AGACAATTTG GCTCAATATC CCAGGACTTA TTTTGAGTCG AATGGGTTTA AGAAGCCATC CTTAAGACTT CCAAAGTCCA ATTTATCTCT AAAAGATCTT AGAGAGATCA TCAAAGGATC ACTCAAAGCA TCTACTATAG ATCCAGAGTA CACTCTTCTC TTCCTGAAAC AATATGTAAC AAGGTTTAAG GCTCAAATAG CAGAGGATTG GATTAGCTTC GATGTTAAAA TCGGCCGAGC AGGAGAAACA ATTAGTCCTC TCAATTTATT TGCAATTGAA GAGTATGACC CCAAACTTAG AGATATGGTC AAAGATACAA CTGCAGAAGA AGATGATGAC CATTGGATGG CCTTTGTCTT GTTATCCATT TATAGGATCT CCAAAGTCCT TAATCCTGCA TATAGAGCAA CCTTAATTGG AAAGATGAAC TCCCAAGCAC AAGGGATCAA TTCTAATGCT TTAAAAGTAC TAGATAATGC AGTAATATTC AATGTATTGA GCAGCAATAA TAGTTATGTA AAATTAGTAG CGGGGACAGA CATGATCCTA TTCAGGATGG GAGATGGAGA ACACAGCCTT TACAGATGGG GAACTGTAGT TACCAGGCAT GAAGATTGCT CAGCCCTCTT GTCCATTGCT CACATATGTA AACTACTGGG GAAAGAAATT GAAGAATTCC TTGAGTGGAT CTTTGTTAAT TCAGTTGGCA GAGATGTAGA AAAAATTCTA GAACCTGGAC AAGAACTTGA CAAACCCTAT TCATATGGTC CATACATGAA GGGACTGGGA TTGGTCACTA GGTCTATATA CTCTGCATCG GCCTGTTCCA ACCTGTACAC ATTTTGTCAT ATGCTGGGGG CAGTCCTTCA TTCAGACAGA TCCAAACATG CTCGTATGAT AAATGAAGGC AATCTAATAA ACATCAAATT AAATACATCT GTTATAGCAT GGGTATTGGG CAGCAGAGGC TCACTAGTCC AGATTTTCGT CAAGAATTCC AGAAAGGATG AGGTCTTGTC TAAACAGACA AAGAATCAAG GGAAAATTGA AACTGATTCT CTTGTTTCCT TTGACGAAGA TGAGATCCCT CAGACCTCTG ATCCTCATGA ATGGTATGAC TACTTGGCAA GACGAGACTT CGACATCCCT GATGTAATAA TGAATGCTGT CAGAAATGAA GCGAAAAAAT TAGTTGGCAC CAGAATTGGG ACAATCGGCC ATTATGTTCA AACCACTTAT TAA
Details for RhbaA.17093.a.D11.PD00303
PURIFICATION DATe: 12/5/2013
CONCENTRATION: 1.9mg/ml
OBSERVED MW: 57kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 30µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMPR TYFESNGFKK PSLRLPKSNL SLKDLREIIK GSLKASTIDP EYTLLFLKQY VTRFKAQIAE DWISFDVKIG RAGETISPLN LFAIEEYDPK LRDMVKDTTA EEDDDHWMAF VLLSIYRISK VLNPAYRATL IGKMNSQAQG INSNALKVLD NAVIFNVLSS NNSYVKLVAG TDMILFRMGD GEHSLYRWGT VVTRHEDCSA LLSIAHICKL LGKEIEEFLE WIFVNSVGRD VEKILEPGQE LDKPYSYGPY MKGLGLVTRS IYSASACSNL YTFCHMLGAV LHSDRSKHAR MINEGNLINI KLNTSVIAWV LGSRGSLVQI FVKNSRKDEV LSKQTKNQGK IETDSLVSFD EDEIPQTSDP HEWYDYLARR DFDIPDVIMN AVRNEAKKLV GTRIGTIGHY VQTTY
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGCCGCGT ACCTATTTTG AATCTAACGG CTTCAAGAAG CCTTCTCTCC GCCTGCCGAA ATCAAACCTG AGCCTCAAGG ATCTGCGTGA AATCATCAAA GGTAGCCTGA AAGCTAGCAC CATCGATCCT GAATATACTC TGCTCTTCCT GAAACAGTAC GTGACCCGCT TCAAAGCACA GATTGCAGAG GATTGGATCA GCTTTGACGT TAAGATCGGC CGTGCTGGCG AAACCATTTC TCCTCTGAAC CTCTTCGCGA TCGAGGAATA TGACCCTAAG TTGCGTGACA TGGTGAAGGA CACTACCGCT GAAGAAGATG ACGATCACTG GATGGCATTC GTTCTGCTGA GCATCTACCG CATCTCCAAG GTGCTGAACC CGGCTTACCG CGCAACTCTG ATAGGCAAGA TGAACTCGCA GGCTCAGGGC ATAAACTCCA ACGCTCTGAA GGTGCTGGAC AACGCTGTTA TCTTCAACGT CCTGTCCTCG AACAATAGCT ATGTTAAATT AGTTGCTGGC ACCGACATGA TCCTGTTTCG TATGGGTGAT GGTGAGCATT CCCTGTACCG TTGGGGCACT GTTGTGACTC GTCACGAAGA CTGTAGCGCT CTGCTGAGCA TCGCCCACAT CTGTAAACTG CTCGGTAAAG AAATCGAAGA ATTTCTGGAG TGGATCTTCG TGAACTCCGT AGGCCGTGAC GTAGAGAAGA TTCTTGAACC GGGCCAAGAA CTCGATAAAC CGTATTCATA CGGCCCTTAC ATGAAAGGCC TGGGTCTCGT GACCCGTAGC ATCTATTCCG CTAGCGCTTG TAGCAACCTC TACACCTTCT GTCACATGTT AGGCGCCGTA CTGCACAGCG ATCGTTCTAA ACACGCCCGT ATGATTAACG AAGGCAACCT GATTAACATT AAACTGAACA CTAGCGTCAT CGCCTGGGTT CTGGGCAGCC GTGGTTCCCT CGTTCAGATT TTCGTTAAGA ATAGCCGTAA AGACGAAGTA CTGAGCAAAC AGACCAAGAA TCAGGGCAAG ATTGAAACTG ATTCCCTGGT TAGCTTCGAC GAGGATGAAA TCCCACAGAC CTCTGACCCG CATGAGTGGT ATGATTACTT AGCTCGCCGC GACTTCGACA TCCCGGATGT TATCATGAAC GCTGTACGTA ACGAGGCTAA GAAACTGGTA GGCACCCGTA TAGGCACTAT CGGTCACTAC GTTCAGACCA CCTACTGATA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT