PsaeA.18889.a

Alanine--tRNA ligase (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS)

CENTER ID: PsaeA.18889.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18889.a.B1.GE38461 full length 1 874
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18889.a.B1.PW37594 full length 1 874

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.938
PGAT: pseudomonas:PA0903
RefSeq: NP_249594.1
UniProt: Q9I553

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSAEIREAF LRFFEEKGHT RVASSSLIPA NDPTLLFTNA GMNQFKDCFL GLEKRAYTRA TTSQKCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKRDAIHYAW EFLTGEKWLN LPKEKLWVTV YATDDEAYDI WTKEVGVPAE RMVRIGDNKG APYASDNFWA MGDTGPCGPC TEIFFDHGPE IWGGPPGSPE EDGDRYIEIW NNVFMQFNRT ADGVMHPLPA PSVDTGMGLE RVSAVLQHVH SNYEIDLFQN LLKASAEAIG CANDDAPSLK VVADHIRSCS FLIADGVLPS NEGRGYVLRR IIRRACRHGN KLGARGTFFH KIVAALVAEM GDAFPELKQQ QAHIERVLKT EEEQFAKTLE QGLKILEQDL AELQGSVIPG NVVFKLYDTY GFPVDLTNDI ARERELTIDE DGFEREMEAQ RERARASSAF GMDYNSLVKV DGETRFLGYQ GVSGAGQIVA LFRDGQAVER LEEGEEGVVV LDQTPFYAES GGQVGDSGYL EAAGVRFDVR DTTKAGGAHL HHGVVARGNL SVGGAVKAEV DASVRQATAL NHSATHLLHA ALRQVLGDHV QQKGSLVDSQ RLRFDFSHFE AIKAEQLKAL EDIVNAEIRR NTEVETEETD IDTAKAKGAM ALFGEKYGDQ VRVLSMGGDF SVELCGGTHV SRTGDIGLFK ITSEGGVAAG VRRIEAVTGA AALAYLNGAE EQLKEAASLV KGSRDNLLDK LGALLERNRS LEKELEQLKA KAASAAGDDL SAAAVEIKGA KVLAARLDGL DGKALLALVD QLKNKLGRAV ILLGGELDGK VVLVAGVTQD LTGQLKAGEL MKQAAAAVGG KGGGRPDMAQ GGGTDAAKLD EALALAQRFV EQGL
NT Sequence
ATGAAAAGCG CTGAAATCCG TGAAGCCTTC CTCCGCTTCT TCGAAGAGAA GGGACACACC CGTGTCGCTT CCAGTTCGCT GATCCCGGCG AACGATCCGA CCCTGCTGTT CACCAACGCG GGCATGAACC AGTTCAAGGA CTGCTTCCTC GGCCTGGAAA AGCGCGCCTA CACCCGTGCG ACCACCAGCC AGAAATGCGT GCGCGCCGGC GGCAAGCACA ACGACCTGGA AAACGTCGGC TACACCGCGC GGCACCACAC CTTCTTCGAA ATGCTGGGTA ACTTCAGCTT CGGCGACTAT TTCAAGCGCG ACGCCATCCA TTACGCCTGG GAGTTCCTCA CCGGCGAGAA ATGGCTGAAC CTGCCCAAGG AAAAGCTCTG GGTCACCGTC TACGCCACCG ACGACGAGGC CTACGACATC TGGACCAAGG AAGTTGGCGT GCCCGCCGAG CGTATGGTGC GGATCGGCGA CAACAAGGGC GCGCCCTATG CGTCCGACAA CTTCTGGGCG ATGGGCGATA CCGGACCCTG CGGTCCCTGC ACCGAGATCT TCTTCGACCA TGGGCCGGAG ATCTGGGGCG GCCCGCCCGG TTCGCCGGAA GAGGACGGCG ACCGCTACAT CGAGATCTGG AACAACGTGT TCATGCAGTT CAACCGCACC GCGGACGGCG TCATGCACCC GCTGCCGGCG CCGAGCGTGG ACACCGGCAT GGGCCTGGAG CGCGTCAGCG CGGTACTCCA GCACGTGCAC TCGAACTATG AGATCGACCT GTTCCAGAAC CTGCTGAAAG CCTCCGCCGA GGCCATCGGC TGCGCCAACG ACGACGCGCC GTCGCTGAAG GTGGTAGCCG ACCACATCCG TTCCTGCAGC TTCCTGATCG CCGACGGCGT GCTGCCGTCC AACGAAGGTC GCGGCTACGT CTTGCGGCGG ATCATCCGCC GCGCCTGCCG CCACGGCAAC AAGCTGGGCG CCAGGGGCAC CTTCTTCCAC AAGATCGTCG CAGCGCTGGT CGCCGAGATG GGCGATGCCT TCCCCGAGCT GAAGCAACAG CAGGCACACA TCGAGCGCGT GCTGAAGACC GAGGAAGAAC AGTTCGCCAA GACCCTGGAG CAAGGCCTGA AGATCCTCGA ACAGGACCTC GCCGAACTGC AGGGCAGCGT GATTCCCGGC AACGTGGTGT TCAAGCTGTA CGACACCTAT GGCTTCCCGG TCGACCTGAC CAATGACATC GCCCGCGAGC GCGAGCTGAC CATCGACGAG GACGGCTTCG AGCGCGAGAT GGAGGCCCAG CGCGAGCGCG CCCGCGCTTC CAGCGCCTTC GGCATGGACT ACAACAGCCT GGTCAAGGTC GACGGCGAGA CCCGCTTCCT CGGCTATCAG GGCGTCTCCG GCGCCGGGCA GATCGTCGCG CTGTTCAGGG ACGGCCAGGC CGTCGAGCGC CTGGAGGAGG GCGAGGAGGG CGTCGTGGTC CTCGACCAGA CGCCGTTCTA CGCCGAGTCC GGCGGCCAGG TCGGCGACAG TGGCTACCTC GAGGCGGCTG GCGTGCGCTT CGACGTGCGC GATACCACCA AGGCTGGCGG CGCGCACCTG CACCATGGCG TGGTCGCCCG GGGCAACCTG AGCGTCGGCG GCGCGGTCAA GGCCGAGGTC GACGCCTCGG TACGCCAGGC CACCGCGCTC AACCACTCCG CGACCCACCT GCTGCATGCC GCGCTGCGCC AGGTACTGGG CGACCACGTA CAACAGAAGG GCTCGCTGGT GGACAGCCAG CGCCTGCGTT TCGACTTCAG CCACTTCGAG GCGATCAAGG CGGAACAACT GAAGGCCCTG GAGGATATCG TCAACGCCGA AATCCGTCGC AACACCGAGG TCGAGACCGA GGAAACCGAT ATCGACACCG CCAAGGCCAA GGGCGCCATG GCGCTGTTCG GCGAGAAGTA CGGCGACCAG GTGCGCGTAC TGAGCATGGG CGGCGATTTC TCCGTCGAGC TGTGCGGTGG CACCCACGTA TCCCGCACCG GCGACATCGG CCTGTTCAAG ATCACCAGCG AGGGTGGCGT GGCTGCTGGC GTACGCCGTA TCGAGGCGGT CACCGGCGCG GCGGCGCTGG CCTACCTGAA CGGTGCCGAG GAGCAGCTCA AGGAAGCCGC CAGCCTGGTC AAGGGCAGCC GCGACAACCT GCTGGACAAG CTCGGCGCCC TGCTGGAGCG CAACCGCTCG CTGGAGAAGG AGCTGGAACA GCTCAAGGCC AAGGCTGCCA GCGCGGCCGG CGACGACCTT TCGGCGGCGG CGGTGGAAAT CAAGGGCGCG AAGGTACTCG CCGCGCGCCT CGATGGTCTG GACGGCAAGG CGCTGCTGGC GCTGGTCGAC CAGTTGAAGA ACAAGCTCGG CCGCGCAGTG ATCCTGCTCG GCGGCGAGCT GGACGGGAAG GTCGTACTGG TCGCAGGCGT GACCCAGGAC CTGACCGGCC AACTGAAGGC CGGCGAGCTT ATGAAGCAAG CCGCGGCGGC AGTTGGTGGC AAAGGTGGCG GACGCCCGGA TATGGCCCAG GGCGGCGGCA CCGATGCAGC GAAATTGGAC GAGGCCCTGG CCCTGGCCCA GCGCTTCGTC GAGCAAGGGC TCTGA
Details for PsaeA.18889.a.B1.GE38461
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 96kDa
OBSERVED MW: 96kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SAEIREAFLR FFEEKGHTRV ASSSLIPAND PTLLFTNAGM NQFKDCFLGL EKRAYTRATT SQKCVRAGGK HNDLENVGYT ARHHTFFEML GNFSFGDYFK RDAIHYAWEF LTGEKWLNLP KEKLWVTVYA TDDEAYDIWT KEVGVPAERM VRIGDNKGAP YASDNFWAMG DTGPCGPCTE IFFDHGPEIW GGPPGSPEED GDRYIEIWNN VFMQFNRTAD GVMHPLPAPS VDTGMGLERV SAVLQHVHSN YEIDLFQNLL KASAEAIGCA NDDAPSLKVV ADHIRSCSFL IADGVLPSNE GRGYVLRRII RRACRHGNKL GARGTFFHKI VAALVAEMGD AFPELKQQQA HIERVLKTEE EQFAKTLEQG LKILEQDLAE LQGSVIPGNV VFKLYDTYGF PVDLTNDIAR ERELTIDEDG FEREMEAQRE RARASSAFGM DYNSLVKVDG ETRFLGYQGV SGAGQIVALF RDGQAVERLE EGEEGVVVLD QTPFYAESGG QVGDSGYLEA AGVRFDVRDT TKAGGAHLHH GVVARGNLSV GGAVKAEVDA SVRQATALNH SATHLLHAAL RQVLGDHVQQ KGSLVDSQRL RFDFSHFEAI KAEQLKALED IVNAEIRRNT EVETEETDID TAKAKGAMAL FGEKYGDQVR VLSMGGDFSV ELCGGTHVSR TGDIGLFKIT SEGGVAAGVR RIEAVTGAAA LAYLNGAEEQ LKEAASLVKG SRDNLLDKLG ALLERNRSLE KELEQLKAKA ASAAGDDLSA AAVEIKGAKV LAARLDGLDG KALLALVDQL KNKLGRAVIL LGGELDGKVV LVAGVTQDLT GQLKAGELMK QAAAAVGGKG GGRPDMAQGG GTDAAKLDEA LALAQRFVEQ GL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa gcgctgaaat ccgtgaagcc ttcctccgct tcttcgaaga gaagggacac acccgtgtcg cttccagttc gctgatcccg gcgaacgatc cgaccctgct gttcaccaac gcgggcatga accagttcaa ggactgcttc ctcggcctgg aaaagcgcgc ctacacccgt gcgaccacca gccagaaatg cgtgcgcgcc ggcggcaagc acaacgacct ggaaaacgtc ggctacaccg cgcggcacca caccttcttc gaaatgctgg gtaacttcag cttcggcgac tatttcaagc gcgacgccat ccattacgcc tgggagttcc tcaccggcga gaaatggctg aacctgccca aggaaaagct ctgggtcacc gtctacgcca ccgacgacga ggcctacgac atctggacca aggaagttgg cgtgcccgcc gagcgtatgg tgcggatcgg cgacaacaag ggcgcgccct atgcgtccga caacttctgg gcgatgggcg ataccggacc ctgcggtccc tgcaccgaga tcttcttcga ccatgggccg gagatctggg gcggcccgcc cggttcgccg gaagaggacg gcgaccgcta catcgagatc tggaacaacg tgttcatgca gttcaaccgc accgcggacg gcgtcatgca cccgctgccg gcgccgagcg tggacaccgg catgggcctg gagcgcgtca gcgcggtact ccagcacgtg cactcgaact atgagatcga cctgttccag aacctgctga aagcctccgc cgaggccatc ggctgcgcca acgacgacgc gccgtcgctg aaggtggtag ccgaccacat ccgttcctgc agcttcctga tcgccgacgg cgtgctgccg tccaacgaag gtcgcggcta cgtcttgcgg cggatcatcc gccgcgcctg ccgccacggc aacaagctgg gcgccagggg caccttcttc cacaagatcg tcgcagcgct ggtcgccgag atgggcgatg ccttccccga gctgaagcaa cagcaggcac acatcgagcg cgtgctgaag accgaggaag aacagttcgc caagaccctg gagcaaggcc tgaagatcct cgaacaggac ctcgccgaac tgcagggcag cgtgattccc ggcaacgtgg tgttcaagct gtacgacacc tatggcttcc cggtcgacct gaccaatgac atcgcccgcg agcgcgagct gaccatcgac gaggacggct tcgagcgcga gatggaggcc cagcgcgagc gcgcccgcgc ttccagcgcc ttcggcatgg actacaacag cctggtcaag gtcgacggcg agacccgctt cctcggctat cagggcgtct ccggcgccgg gcagatcgtc gcgctgttca gggacggcca ggccgtcgag cgcctggagg agggcgagga gggcgtcgtg gtcctcgacc agacgccgtt ctacgccgag tccggcggcc aggtcggcga cagtggctac ctcgaggcgg ctggcgtgcg cttcgacgtg cgcgatacca ccaaggctgg cggcgcgcac ctgcaccatg gcgtggtcgc ccggggcaac ctgagcgtcg gcggcgcggt caaggccgag gtcgacgcct cggtacgcca ggccaccgcg ctcaaccact ccgcgaccca cctgctgcat gccgcgctgc gccaggtact gggcgaccac gtacaacaga agggctcgct ggtggacagc cagcgcctgc gtttcgactt cagccacttc gaggcgatca aggcggaaca actgaaggcc ctggaggata tcgtcaacgc cgaaatccgt cgcaacaccg aggtcgagac cgaggaaacc gatatcgaca ccgccaaggc caagggcgcc atggcgctgt tcggcgagaa gtacggcgac caggtgcgcg tactgagcat gggcggcgat ttctccgtcg agctgtgcgg tggcacccac gtatcccgca ccggcgacat cggcctgttc aagatcacca gcgagggtgg cgtggctgct ggcgtacgcc gtatcgaggc ggtcaccggc gcggcggcgc tggcctacct gaacggtgcc gaggagcagc tcaaggaagc cgccagcctg gtcaagggca gccgcgacaa cctgctggac aagctcggcg ccctgctgga gcgcaaccgc tcgctggaga aggagctgga acagctcaag gccaaggctg ccagcgcggc cggcgacgac ctttcggcgg cggcggtgga aatcaagggc gcgaaggtac tcgccgcgcg cctcgatggt ctggacggca aggcgctgct ggcgctggtc gaccagttga agaacaagct cggccgcgca gtgatcctgc tcggcggcga gctggacggg aaggtcgtac tggtcgcagg cgtgacccag gacctgaccg gccaactgaa ggccggcgag cttatgaagc aagccgcggc ggcagttggt ggcaaaggtg gcggacgccc ggatatggcc cagggcggcg gcaccgatgc agcgaaattg gacgaggccc tggccctggc ccagcgcttc gtcgagcaag ggctctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for PsaeA.18889.a.B1.PW37594
PURIFICATION DATe: 11/11/2014
CONCENTRATION: 19mg/ml
OBSERVED MW: 95kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK SAEIREAFLR FFEEKGHTRV ASSSLIPAND PTLLFTNAGM NQFKDCFLGL EKRAYTRATT SQKCVRAGGK HNDLENVGYT ARHHTFFEML GNFSFGDYFK RDAIHYAWEF LTGEKWLNLP KEKLWVTVYA TDDEAYDIWT KEVGVPAERM VRIGDNKGAP YASDNFWAMG DTGPCGPCTE IFFDHGPEIW GGPPGSPEED GDRYIEIWNN VFMQFNRTAD GVMHPLPAPS VDTGMGLERV SAVLQHVHSN YEIDLFQNLL KASAEAIGCA NDDAPSLKVV ADHIRSCSFL IADGVLPSNE GRGYVLRRII RRACRHGNKL GARGTFFHKI VAALVAEMGD AFPELKQQQA HIERVLKTEE EQFAKTLEQG LKILEQDLAE LQGSVIPGNV VFKLYDTYGF PVDLTNDIAR ERELTIDEDG FEREMEAQRE RARASSAFGM DYNSLVKVDG ETRFLGYQGV SGAGQIVALF RDGQAVERLE EGEEGVVVLD QTPFYAESGG QVGDSGYLEA AGVRFDVRDT TKAGGAHLHH GVVARGNLSV GGAVKAEVDA SVRQATALNH SATHLLHAAL RQVLGDHVQQ KGSLVDSQRL RFDFSHFEAI KAEQLKALED IVNAEIRRNT EVETEETDID TAKAKGAMAL FGEKYGDQVR VLSMGGDFSV ELCGGTHVSR TGDIGLFKIT SEGGVAAGVR RIEAVTGAAA LAYLNGAEEQ LKEAASLVKG SRDNLLDKLG ALLERNRSLE KELEQLKAKA ASAAGDDLSA AAVEIKGAKV LAARLDGLDG KALLALVDQL KNKLGRAVIL LGGELDGKVV LVAGVTQDLT GQLKAGELMK QAAAAVGGKG GGRPDMAQGG GTDAAKLDEA LALAQRFVEQ GL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA GCGCTGAAAT CCGTGAAGCC TTCCTCCGCT TCTTCGAAGA GAAGGGACAC ACCCGTGTCG CTTCCAGTTC GCTGATCCCG GCGAACGATC CGACCCTGCT GTTCACCAAC GCGGGCATGA ACCAGTTCAA GGACTGCTTC CTCGGCCTGG AAAAGCGCGC CTACACCCGT GCGACCACCA GCCAGAAATG CGTGCGCGCC GGCGGCAAGC ACAACGACCT GGAAAACGTC GGCTACACCG CGCGGCACCA CACCTTCTTC GAAATGCTGG GTAACTTCAG CTTCGGCGAC TATTTCAAGC GCGACGCCAT CCATTACGCC TGGGAGTTCC TCACCGGCGA GAAATGGCTG AACCTGCCCA AGGAAAAGCT CTGGGTCACC GTCTACGCCA CCGACGACGA GGCCTACGAC ATCTGGACCA AGGAAGTTGG CGTGCCCGCC GAGCGTATGG TGCGGATCGG CGACAACAAG GGCGCGCCCT ATGCGTCCGA CAACTTCTGG GCGATGGGCG ATACCGGACC CTGCGGTCCC TGCACCGAGA TCTTCTTCGA CCATGGGCCG GAGATCTGGG GCGGCCCGCC CGGTTCGCCG GAAGAGGACG GCGACCGCTA CATCGAGATC TGGAACAACG TGTTCATGCA GTTCAACCGC ACCGCGGACG GCGTCATGCA CCCGCTGCCG GCGCCGAGCG TGGACACCGG CATGGGCCTG GAGCGCGTCA GCGCGGTACT CCAGCACGTG CACTCGAACT ATGAGATCGA CCTGTTCCAG AACCTGCTGA AAGCCTCCGC CGAGGCCATC GGCTGCGCCA ACGACGACGC GCCGTCGCTG AAGGTGGTAG CCGACCACAT CCGTTCCTGC AGCTTCCTGA TCGCCGACGG CGTGCTGCCG TCCAACGAAG GTCGCGGCTA CGTCTTGCGG CGGATCATCC GCCGCGCCTG CCGCCACGGC AACAAGCTGG GCGCCAGGGG CACCTTCTTC CACAAGATCG TCGCAGCGCT GGTCGCCGAG ATGGGCGATG CCTTCCCCGA GCTGAAGCAA CAGCAGGCAC ACATCGAGCG CGTGCTGAAG ACCGAGGAAG AACAGTTCGC CAAGACCCTG GAGCAAGGCC TGAAGATCCT CGAACAGGAC CTCGCCGAAC TGCAGGGCAG CGTGATTCCC GGCAACGTGG TGTTCAAGCT GTACGACACC TATGGCTTCC CGGTCGACCT GACCAATGAC ATCGCCCGCG AGCGCGAGCT GACCATCGAC GAGGACGGCT TCGAGCGCGA GATGGAGGCC CAGCGCGAGC GCGCCCGCGC TTCCAGCGCC TTCGGCATGG ACTACAACAG CCTGGTCAAG GTCGACGGCG AGACCCGCTT CCTCGGCTAT CAGGGCGTCT CCGGCGCCGG GCAGATCGTC GCGCTGTTCA GGGACGGCCA GGCCGTCGAG CGCCTGGAGG AGGGCGAGGA GGGCGTCGTG GTCCTCGACC AGACGCCGTT CTACGCCGAG TCCGGCGGCC AGGTCGGCGA CAGTGGCTAC CTCGAGGCGG CTGGCGTGCG CTTCGACGTG CGCGATACCA CCAAGGCTGG CGGCGCGCAC CTGCACCATG GCGTGGTCGC CCGGGGCAAC CTGAGCGTCG GCGGCGCGGT CAAGGCCGAG GTCGACGCCT CGGTACGCCA GGCCACCGCG CTCAACCACT CCGCGACCCA CCTGCTGCAT GCCGCGCTGC GCCAGGTACT GGGCGACCAC GTACAACAGA AGGGCTCGCT GGTGGACAGC CAGCGCCTGC GTTTCGACTT CAGCCACTTC GAGGCGATCA AGGCGGAACA ACTGAAGGCC CTGGAGGATA TCGTCAACGC CGAAATCCGT CGCAACACCG AGGTCGAGAC CGAGGAAACC GATATCGACA CCGCCAAGGC CAAGGGCGCC ATGGCGCTGT TCGGCGAGAA GTACGGCGAC CAGGTGCGCG TACTGAGCAT GGGCGGCGAT TTCTCCGTCG AGCTGTGCGG TGGCACCCAC GTATCCCGCA CCGGCGACAT CGGCCTGTTC AAGATCACCA GCGAGGGTGG CGTGGCTGCT GGCGTACGCC GTATCGAGGC GGTCACCGGC GCGGCGGCGC TGGCCTACCT GAACGGTGCC GAGGAGCAGC TCAAGGAAGC CGCCAGCCTG GTCAAGGGCA GCCGCGACAA CCTGCTGGAC AAGCTCGGCG CCCTGCTGGA GCGCAACCGC TCGCTGGAGA AGGAGCTGGA ACAGCTCAAG GCCAAGGCTG CCAGCGCGGC CGGCGACGAC CTTTCGGCGG CGGCGGTGGA AATCAAGGGC GCGAAGGTAC TCGCCGCGCG CCTCGATGGT CTGGACGGCA AGGCGCTGCT GGCGCTGGTC GACCAGTTGA AGAACAAGCT CGGCCGCGCA GTGATCCTGC TCGGCGGCGA GCTGGACGGG AAGGTCGTAC TGGTCGCAGG CGTGACCCAG GACCTGACCG GCCAACTGAA GGCCGGCGAG CTTATGAAGC AAGCCGCGGC GGCAGTTGGT GGCAAAGGTG GCGGACGCCC GGATATGGCC CAGGGCGGCG GCACCGATGC AGCGAAATTG GACGAGGCCC TGGCCCTGGC CCAGCGCTTC GTCGAGCAAG GGCTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT