PsaeA.18887.a

Arabinose 5-phosphate isomerase KdsD (API) (EC 5.3.1.13) (Pa-KdsD)

CENTER ID: PsaeA.18887.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18887.a.B1.GE38459 full length 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4667
PGAT: pseudomonas:PA4457
RefSeq: NP_253147.1
UniProt: Q9HVW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNMSQNLDFI HSAQRTIGLE RDAVDSLLAR IGDDFVKACE LLLAGKGRVV VVGMGKSGHV GKKIAATLAS TGTPSFFVHP AEASHGDMGM ITKDDVVLAL SNSGSTAEIV TLLPLIKRLG ITLISMTGNP ESPLAKAAEV NLDASVGQEA CPLNLAPTSS TTVTLVLGDA LAIALLEARG FTAEDFAFSH PGGALGRRLL LKVEDVMHVG EGLPQVLLGT SLTGALMEMT RKGLGMTVVL DEHGKLAGIF TDGDLRRALD RGIDVRQVTI DQVMTVHGKT VRAEILAAEA LKIMEDNKIG ALVVVDADDR PVGALNMHDL LRAGVM
NT Sequence
ttgaacatga gccagaacct cgatttcatc cattccgccc agcgcaccat cggcctcgag cgcgacgcgg tagacagcct gctggcgcgg attggcgacg atttcgtcaa ggcctgcgaa ctgctgctgg ccggcaaggg ccgggtcgtc gtcgtcggca tgggcaaatc cggccacgtc ggcaaaaaga tcgccgctac cctggccagc accggcaccc cctccttctt cgtgcatccg gccgaggcca gccatggcga catgggcatg atcaccaagg acgacgtggt cctcgccctg tccaactccg gctcgactgc cgaaatcgtc accctgttgc ctctgatcaa gcgcctgggc atcaccctga tcagcatgac cggtaacccc gagtcgcccc tggccaaggc tgcggaagtg aacctggacg ccagtgtcgg ccaggaagcc tgcccactga acctggcccc gacctcctcc accaccgtaa ccctggtcct cggcgacgcc ctggcgatcg ccctgctcga agcccgcggc tttaccgcgg aagacttcgc cttctcgcac ccgggcggtg cgctgggccg ccgactgctg ctgaaggtgg aagacgtcat gcacgtcggc gaagggctgc cgcaggtgct gctcggcacc tcgctgaccg gcgcgctgat ggaaatgacc cgcaagggcc tgggcatgac cgtagtgctc gacgaacatg gcaagcttgc cggcattttc accgatggcg acctgcgtcg cgccctggat cggggcatcg acgtccgcca ggtcaccatc gaccaggtga tgaccgtcca cggcaagacc gtccgcgccg agatcctcgc cgcggaagcc ctgaagatca tggaagacaa caagatcggc gcactggtgg tggtcgacgc ggacgaccgt ccggtcggcg ccctgaacat gcacgacctg ttgcgcgcag gagtgatgta a
Details for PsaeA.18887.a.B1.GE38459
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 35kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML NMSQNLDFIH SAQRTIGLER DAVDSLLARI GDDFVKACEL LLAGKGRVVV VGMGKSGHVG KKIAATLAST GTPSFFVHPA EASHGDMGMI TKDDVVLALS NSGSTAEIVT LLPLIKRLGI TLISMTGNPE SPLAKAAEVN LDASVGQEAC PLNLAPTSST TVTLVLGDAL AIALLEARGF TAEDFAFSHP GGALGRRLLL KVEDVMHVGE GLPQVLLGTS LTGALMEMTR KGLGMTVVLD EHGKLAGIFT DGDLRRALDR GIDVRQVTID QVMTVHGKTV RAEILAAEAL KIMEDNKIGA LVVVDADDRP VGALNMH
Validated NT Sequence
tcgtgcatgt tcagggcgcc gaccggacgg tcgtccgcgt cgaccaccac cagtgcgccg atcttgttgt cttccatgat cttcagggct tccgcggcga ggatctcggc gcggacggtc ttgccgtgga cggtcatcac ctggtcgatg gtgacctggc ggacgtcgat gccccgatcc agggcgcgac gcaggtcgcc atcggtgaaa atgccggcaa gcttgccatg ttcgtcgagc actacggtca tgcccaggcc cttgcgggtc atttccatca gcgcgccggt cagcgaggtg ccgagcagca cctgcggcag cccttcgccg acgtgcatga cgtcttccac cttcagcagc agtcggcggc ccagcgcacc gcccgggtgc gagaaggcga agtcttccgc ggtaaagccg cgggcttcga gcagggcgat cgccagggcg tcgccgagga ccagggttac ggtggtggag gaggtcgggg ccaggttcag tgggcaggct tcctggccga cactggcgtc caggttcact tccgcagcct tggccagggg cgactcgggg ttaccggtca tgctgatcag ggtgatgccc aggcgcttga tcagaggcaa cagggtgacg atttcggcag tcgagccgga gttggacagg gcgaggacca cgtcgtcctt ggtgatcatg cccatgtcgc catggctggc ctcggccgga tgcacgaaga aggagggggt gccggtgctg gccagggtag cggcgatctt tttgccgacg tggccggatt tgcccatgcc gacgacgacg acccggccct tgccggccag cagcagttcg caggccttga cgaaatcgtc gccaatccgc gccagcaggc tgtctaccgc gtcgcgctcg aggccgatgg tgcgctgggc ggaatggatg aaatcgaggt tctggctcat gttcaacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMN MSQNLDFIHS AQRTIGLERD AVDSLLARIG DDFVKACELL LAGKGRVVVV GMGKSGHVGK KIAATLASTG TPSFFVHPAE ASHGDMGMIT KDDVVLALSN SGSTAEIVTL LPLIKRLGIT LISMTGNPES PLAKAAEVNL DASVGQEACP LNLAPTSSTT VTLVLGDALA IALLEARGFT AEDFAFSHPG GALGRRLLLK VEDVMHVGEG LPQVLLGTSL TGALMEMTRK GLGMTVVLDE HGKLAGIFTD GDLRRALDRG IDVRQVTIDQ VMTVHGKTVR AEILAAEALK IMEDNKIGAL VVVDADDRPV GALNMHDLLR AGVM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaca tgagccagaa cctcgatttc atccattccg cccagcgcac catcggcctc gagcgcgacg cggtagacag cctgctggcg cggattggcg acgatttcgt caaggcctgc gaactgctgc tggccggcaa gggccgggtc gtcgtcgtcg gcatgggcaa atccggccac gtcggcaaaa agatcgccgc taccctggcc agcaccggca ccccctcctt cttcgtgcat ccggccgagg ccagccatgg cgacatgggc atgatcacca aggacgacgt ggtcctcgcc ctgtccaact ccggctcgac tgccgaaatc gtcaccctgt tgcctctgat caagcgcctg ggcatcaccc tgatcagcat gaccggtaac cccgagtcgc ccctggccaa ggctgcggaa gtgaacctgg acgccagtgt cggccaggaa gcctgcccac tgaacctggc cccgacctcc tccaccaccg taaccctggt cctcggcgac gccctggcga tcgccctgct cgaagcccgc ggctttaccg cggaagactt cgccttctcg cacccgggcg gtgcgctggg ccgccgactg ctgctgaagg tggaagacgt catgcacgtc ggcgaagggc tgccgcaggt gctgctcggc acctcgctga ccggcgcgct gatggaaatg acccgcaagg gcctgggcat gaccgtagtg ctcgacgaac atggcaagct tgccggcatt ttcaccgatg gcgacctgcg tcgcgccctg gatcggggca tcgacgtccg ccaggtcacc atcgaccagg tgatgaccgt ccacggcaag accgtccgcg ccgagatcct cgccgcggaa gccctgaaga tcatggaaga caacaagatc ggcgcactgg tggtggtcga cgcggacgac cgtccggtcg gcgccctgaa catgcacgac ctgttgcgcg caggagtgat gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat