PsaeA.18883.a

Octanoyltransferase (EC 2.3.1.181) (Lipoate-protein ligase B) (Lipoyl/octanoyl transferase) (Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase)

CENTER ID: PsaeA.18883.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18883.a.B1.GE38456 full length 1 217
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4189
PGAT: pseudomonas:PA3997
RefSeq: NP_252686.1
UniProt: Q9X6V9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLELGFREL GEVPYEPTWH AMQRFVAERD KSVMDEAWLL QHPAVFTQGQ AGKAEHVLFP GDIPVIQVDR GGQVTYHGPG QLVTYLLLDV RRLGLGVREL VSRIEQSLIG LLASYDVQAV AKPDAPGVYV DGAKIASLGL RIRNGCSFHG LALNLDMDLR PFQRINPCGY AGMPMTQLRD LVGPVDFAEV CTRLRAELVS RLGYAEQKTL TGGIELT
NT Sequence
ATGGGACTCG AGCTGGGCTT CCGTGAGCTC GGCGAGGTTC CCTACGAACC GACCTGGCAT GCCATGCAGC GCTTCGTTGC CGAGCGCGAC AAATCGGTAA TGGACGAAGC CTGGCTGTTG CAGCACCCTG CGGTCTTCAC CCAGGGGCAG GCCGGCAAGG CGGAGCATGT GCTGTTCCCC GGCGACATCC CGGTGATCCA GGTAGATCGC GGCGGCCAGG TGACCTACCA CGGCCCCGGC CAGTTGGTCA CCTACCTGCT GCTCGATGTC CGCCGCCTGG GACTGGGCGT GCGCGAGCTG GTCAGCCGGA TCGAGCAGAG CCTGATCGGC CTGCTGGCGT CCTATGACGT GCAGGCCGTG GCCAAGCCGG ACGCGCCGGG TGTCTACGTC GACGGGGCGA AGATCGCCTC CCTCGGCCTG CGCATCCGCA ACGGCTGTTC CTTCCATGGC CTGGCGCTGA ACCTGGACAT GGACCTGCGA CCGTTCCAAC GAATCAATCC CTGCGGCTAC GCCGGGATGC CCATGACCCA ACTGCGCGAC CTGGTTGGGC CGGTGGATTT TGCCGAGGTG TGTACCCGAT TGCGCGCTGA GCTCGTCTCA CGCCTTGGCT ACGCTGAACA GAAGACCCTT ACGGGCGGGA TCGAACTGAC ATGA
Details for PsaeA.18883.a.B1.GE38456
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG LELGFRELGE VPYEPTWHAM QRFVAERDKS VMDEAWLLQH PAVFTQGQAG KAEHVLFPGD IPVIQVDRGG QVTYHGPGQL VTYLLLDVRR LGLGVRELVS RIEQSLIGLL ASYDVQAVAK PDAPGVYVDG AKIASLGLRI RXGCSFHGLA LNLDMDLRPF QRINPCGYAG MPMTQLRDLV GPVDFAEVCT RLRAELVSRL GYAEQKTLTG GIELT
Validated NT Sequence
atcttactca cttatgtcag ttcgatcccg cccgtaaggg tcttctgttc agcgtagcca aggcgtgaga cgagctcagc gcgcaatcgg gtacacacct cggcaaaatc caccggccca accaggtcgc gcagttgggt catgggcatc ccggcgtagc cgcagggatt gattcgttgg aacggtcgca ggtccatgtc caggttcagc gccaggccat ggaaggaaca gccgntgcgg atgcgcaggc cgagggaggc gatcttcgcc ccgtcgacgt agacacccgg cgcgtccggc ttggccacgg cctgcacgtc ataggacgcc agcaggccga tcaggctctg ctcgatccgg ctgaccagct cgcgcacgcc cagtcccagg cggcggacat cgagcagcag gtaggtgacc aactggccgg ggccgtggta ggtcacctgg ccgccgcgat ctacctggat caccgggatg tcgccgggga acagcacatg ctccgccttg ccggcctgcc cctgggtgaa gaccgcaggg tgctgcaaca gccaggcttc gtccattacc gatttgtcgc gctcggcaac gaagcgctgc atggcatgcc aggtcggttc gtagggaacc tcgccgagct cacggaagcc cagctcgagt cccatatggt ggtggtggtg gtgagccatn gtatat
Expected Protein Sequence
MAHHHHHHMG LELGFRELGE VPYEPTWHAM QRFVAERDKS VMDEAWLLQH PAVFTQGQAG KAEHVLFPGD IPVIQVDRGG QVTYHGPGQL VTYLLLDVRR LGLGVRELVS RIEQSLIGLL ASYDVQAVAK PDAPGVYVDG AKIASLGLRI RNGCSFHGLA LNLDMDLRPF QRINPCGYAG MPMTQLRDLV GPVDFAEVCT RLRAELVSRL GYAEQKTLTG GIELT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac tcgagctggg cttccgtgag ctcggcgagg ttccctacga accgacctgg catgccatgc agcgcttcgt tgccgagcgc gacaaatcgg taatggacga agcctggctg ttgcagcacc ctgcggtctt cacccagggg caggccggca aggcggagca tgtgctgttc cccggcgaca tcccggtgat ccaggtagat cgcggcggcc aggtgaccta ccacggcccc ggccagttgg tcacctacct gctgctcgat gtccgccgcc tgggactggg cgtgcgcgag ctggtcagcc ggatcgagca gagcctgatc ggcctgctgg cgtcctatga cgtgcaggcc gtggccaagc cggacgcgcc gggtgtctac gtcgacgggg cgaagatcgc ctccctcggc ctgcgcatcc gcaacggctg ttccttccat ggcctggcgc tgaacctgga catggacctg cgaccgttcc aacgaatcaa tccctgcggc tacgccggga tgcccatgac ccaactgcgc gacctggttg ggccggtgga ttttgccgag gtgtgtaccc gattgcgcgc tgagctcgtc tcacgccttg gctacgctga acagaagacc cttacgggcg ggatcgaact gacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat