PsaeA.18881.a

Apolipoprotein N-acyltransferase (ALP N-acyltransferase) (EC 2.3.1.-) (Copper homeostasis protein CutE homolog)

CENTER ID: PsaeA.18881.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18881.a.B2.GE38454 soluble domain 212 480
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4176
PGAT: pseudomonas:PA3984
RefSeq: NP_252673.1
UniProt: Q9ZI86

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRWISRPGWP GHLLALAAGA LTPLALAPFD YWPLAILSIA LLYLGLRGLP GKSALWRGWW YGFGAFGAGT SWIYVSIHDY GAASVPLASL LMLGFTAGVA FFFALPAWLW ARCLRRDNAP LGDALAFAAL WLALELFRSW FLTGFPWLYA GYSQLQGPLA GLVPVGGVWL SSFVIALSAA LLVNLPRLFP HGASLLLGLV LLLGPWAAGL YLKGHAWTHS AGEPLRVVAI QGNIAQELKW DPNQVRAQLD LYRDLSLPQQ DVDLIVWPET AVPILQDMAS GYLGAMGQVA DEKNAALITG VPVRERLADG KSRYFNGITV VGEGAGTYLK QKLVPFGEYV PLQDLLRGLI AFFDLPMSDF ARGPADQPLL KAKGYQIAPY ICYEVVYPEF AAALAAQSQV LLTVSNDTWF GTSIGPLQHL QMAQMRALES GRWMIRATNN GVTGLIDPYG RIVRQIPQFQ QGILRGEVIP MQGLTPYLQY RVWPLAGLAG VLLLWALLGR QLRPQERRLF G
NT Sequence
atgcgttgga tttctcgtcc cggctggccc ggacacctgc tggcactcgc cgcaggcgcg ctcacacccc tggccctggc gcccttcgac tactggccgc tggcgatcct ctcgatcgcc ctgctctatc tcggcttgcg cggccttccc ggcaagtccg ccctgtggcg cggctggtgg tacggtttcg gcgcgttcgg cgccggcacc agctggatct acgtcagtat ccatgactac ggcgccgcct cggtgccgct ggcaagcctc ctcatgctcg gcttcaccgc cggcgtcgcc ttcttcttcg ccctgccggc ctggctgtgg gcgcgctgcc tgcgccgcga caacgcgccg ctgggcgacg ccctggcgtt cgccgccctg tggctggcac tggagctgtt ccgcagttgg ttccttaccg gtttcccctg gctctacgcc ggctatagcc agttgcaggg accgctggcc gggctggtac cggtgggcgg cgtatggctg tcctccttcg tcatcgccct cagcgcggcc ctgctggtga acctgccgcg cctcttcccg cacggtgcca gcctgcttct ggggctggtc ctgctgctcg gcccgtgggc ggccggcctc tatctcaagg gccacgcctg gacccattcg gccggcgaac cgctgagggt agtggcgatc cagggcaaca tcgcccagga gctgaaatgg gacccgaatc aggtccgcgc gcaactcgac ctctaccgcg acctgagcct gccgcagcag gacgtcgacc tgatcgtctg gccggaaacc gcggtgccga ttctccagga catggccagc ggctacctcg gcgccatggg ccaggtcgcc gacgagaaga acgccgcgct gatcaccggg gtcccggtgc gtgaacgtct cgccgacggc aagagtcgct acttcaacgg catcaccgtg gtcggcgaag gcgcgggcac ctacctcaag cagaagctgg tgccgttcgg cgagtacgtg ccgctgcagg acctgctgcg cggcctgatc gccttcttcg acctgccgat gtccgacttc gctcgcggtc cggccgacca gccgctgctc aaggccaagg gctaccagat cgccccgtac atctgctacg aagtggtcta cccggagttc gccgccgcgc tcgcggcgca gagccaggtg ttgctgacag tgagcaacga cacctggttc ggcacttcca taggcccttt gcagcacctg cagatggcac agatgcgcgc cctggaaagc ggtcgctgga tgatccgcgc gaccaacaac ggcgtcaccg gcctgatcga tccctatggc cggatcgtcc ggcagatccc gcagttccag cagggcatcc tgcgcggcga ggtgattccc atgcagggcc tgacgcccta tctgcaatac cgcgtctggc cgctggccgg gttggccggc gtgctgctgc tatgggctct gctcggccgc cagttgcgtc cgcaggagcg acgcctgttc ggctga
Details for PsaeA.18881.a.B2.GE38454
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KGHAWTHSAG EPLRVVAIQG NIAQELKWDP NQVRAQLDLY RDLSLPQQDV DLIVWPETAV PILQDMASGY LGAMGQVADE KNAALITGVP VRERLADGKS RYFNGITVVG EGAGTYLKQK LVPFGEYVPL QDLLRGLIAF FDLPMSDFAR GPADQPLLKA KGYQIAPYIC YEVVYPEFAA ALAAQSQVLL TVSNDTWFGT SIGPLQHLQM AQMRALESGR WMIRATNNGV TGLIDPYGRI VRQIPQFQQG ILRGEVIPMQ XXXPYLQY
Validated NT Sequence
atcttactca cttagtattg cagatagggc ntcannnnct gcatgggaat cacctcgccg cgcaggatgc cctgctggaa ctgcgggatc tgccggacga tccggccata gggatcgatc aggccggtga cgccgttgtt ggtcgcgcgg atcatccagc gaccgctttc cagggcgcgc atctgtgcca tctgcaggtg ctgcaaaggg cctatggaag tgccgaacca ggtgtcgttg ctcactgtca gcaacacctg gctctgcgcc gcgagcgcgg cggcgaactc cgggtagacc acttcgtagc agatgtacgg ggcgatctgg tagcccttgg ccttgagcag cggctggtcg gccggaccgc gagcgaagtc ggacatcggc aggtcgaaga aggcgatcag gccgcgcagc aggtcctgca gcggcacgta ctcgccgaac ggcaccagct tctgcttgag gtaggtgccc gcgccttcgc cgaccacggt gatgccgttg aagtagcgac tcttgccgtc ggcgagacgt tcacgcaccg ggaccccggt gatcagcgcg gcgttcttct cgtcggcgac ctggcccatg gcgccgaggt agccgctggc catgtcctgg agaatcggca ccgcggtttc cggccagacg atcaggtcga cgtcctgctg cggcaggctc aggtcgcggt agaggtcgag ttgcgcgcgg acctgattcg ggtcccattt cagctcctgg gcgatgttgc cctggatcgc cactaccctc agcggttcgc cggccgaatg ggtccaggcg tggcccttga gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHLK GHAWTHSAGE PLRVVAIQGN IAQELKWDPN QVRAQLDLYR DLSLPQQDVD LIVWPETAVP ILQDMASGYL GAMGQVADEK NAALITGVPV RERLADGKSR YFNGITVVGE GAGTYLKQKL VPFGEYVPLQ DLLRGLIAFF DLPMSDFARG PADQPLLKAK GYQIAPYICY EVVYPEFAAA LAAQSQVLLT VSNDTWFGTS IGPLQHLQMA QMRALESGRW MIRATNNGVT GLIDPYGRIV RQIPQFQQGI LRGEVIPMQG LTPYLQY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcaagg gccacgcctg gacccattcg gccggcgaac cgctgagggt agtggcgatc cagggcaaca tcgcccagga gctgaaatgg gacccgaatc aggtccgcgc gcaactcgac ctctaccgcg acctgagcct gccgcagcag gacgtcgacc tgatcgtctg gccggaaacc gcggtgccga ttctccagga catggccagc ggctacctcg gcgccatggg ccaggtcgcc gacgagaaga acgccgcgct gatcaccggg gtcccggtgc gtgaacgtct cgccgacggc aagagtcgct acttcaacgg catcaccgtg gtcggcgaag gcgcgggcac ctacctcaag cagaagctgg tgccgttcgg cgagtacgtg ccgctgcagg acctgctgcg cggcctgatc gccttcttcg acctgccgat gtccgacttc gctcgcggtc cggccgacca gccgctgctc aaggccaagg gctaccagat cgccccgtac atctgctacg aagtggtcta cccggagttc gccgccgcgc tcgcggcgca gagccaggtg ttgctgacag tgagcaacga cacctggttc ggcacttcca taggcccttt gcagcacctg cagatggcac agatgcgcgc cctggaaagc ggtcgctgga tgatccgcgc gaccaacaac ggcgtcaccg gcctgatcga tccctatggc cggatcgtcc ggcagatccc gcagttccag cagggcatcc tgcgcggcga ggtgattccc atgcagggcc tgacgcccta tctgcaatac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat