SSGCID: Target Page

PsaeA.18877.a

Membrane protein insertase YidC (Foldase YidC) (Membrane integrase YidC) (Membrane protein YidC)

CENTER ID:	PsaeA.18877.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18877.a.B2.GE38450	soluble domain		23	360

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18877.a.B2.PW37583	soluble domain		23	360

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5834
PGAT:	pseudomonas:PA5568
RefSeq:	NP_254255.1
UniProt:	Q9HT06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDIQRSILIV ALAVVSYLLV LQWNKDYGQP ELPAASASMN TTQGLPDTPS ASGTSSDVPT AQSSAAGSEA ADKPVAVSDK LIQVKTDVLD LAIDPRGGDI VQLGLLQYPR RLDRPDVPFP LFDNGRERTY LAQSGLTGAD GPDASSAGRP LFRSAQSSYQ LADGQNELVV DLSFSHDGVN YIKRFTFHRG LKADCSDKEK AQKKIECINE NAYQVGVSYL IDNQSGKTWS GNLFAQLKRD GSADPSSTTA TGVSTYLGAA VWTPDSPYKK ISTKDMDKEQ FKESVQGGWV AWLQHYFVTA WVPTKGEQHQ VMTRKDGQGN YIVGFTGPTL SVPAGSKVET DLTLYAGPKL QKHLKELSPG LELTVDYGFL WFIAQPIFWL LQHIHSLIGN WGWSIIALTV LIKLAFFPLS AASYRSMARM RAVSPKMQAI KEQHGDDRQK MSQAMMELYK KEKINPLGGC LPILVQMPVF LSLYWVLLES VEMRQAPWLG WITDLSVKDP FFILPIVMGG TMLIQQMLNP TPPDPMQAKV MKLMPIIFTF FFLWFPAGLV LYWVVNNCLS IAQQWYITRK IEAAAKTA

NT Sequence

atggacattc aacgctcgat tctcatcgtc gcccttgcag tcgtgtccta cctgctggtt ctccagtgga acaaggatta cggtcagcct gagctgcccg ccgccagcgc ttcgatgaac accacgcaag gcctgccgga cactccgtcc gcctcgggca ccagcagtga cgttcccact gcccagagca gcgcggccgg cagcgaagcc gccgacaagc cggttgcggt cagcgacaag ctgatccagg tgaagaccga cgtcctcgac ctggccatcg atccccgtgg cggcgatatc gtccagctcg gcctgttgca gtatccgcgc cgcctggacc gtccggacgt gccgttcccg ctgttcgaca acggtcgcga acgtacctac ctggcgcaga gcggtctcac cggtgccgac ggcccggacg ccagcagcgc cggccgccca ctgttccgca gtgcgcagag cagctaccag ttggccgacg gccagaacga actggtcgtg gacctgagct tcagccacga cggtgtcaac tacatcaagc gcttcacctt ccaccgtggc ctgaaagccg attgctcgga caaggaaaag gcccagaaga agatcgagtg catcaatgaa aacgcctatc aggtcggcgt cagctacctg atcgacaacc agagcggcaa gacctggagc ggcaacctgt tcgcccagct caagcgcgac ggcagcgccg acccgtcctc gaccaccgcc accggggtgt ccacctacct cggcgccgcg gtctggaccc cggattcgcc gtacaagaag atctccacca aggacatgga caaggaacag ttcaaggagt ccgtgcaggg tggctgggtc gcctggctgc aacactactt cgtgaccgcc tgggttccga ccaagggcga gcagcaccag gtcatgaccc gcaaggacgg ccagggcaac tacatcgtcg gtttcaccgg cccgaccctg agcgttccgg cgggtagcaa ggtggaaacc gacctgaccc tgtatgccgg cccgaaactc cagaagcacc tgaaggaact ctccccgggc ctggaactga ccgtcgacta cggtttcctt tggttcatcg cccagccgat cttctggctg ctgcaacata tccacagcct gatcggcaac tggggctggt cgatcatcgc cctgaccgtc ctgatcaagc tggccttctt cccgttgtcc gccgccagct accgttcgat ggcgcgcatg cgcgcggtgt cgccgaagat gcaggcgatc aaggaacagc acggcgacga tcggcagaag atgtcccagg cgatgatgga gctgtacaag aaggagaaga tcaatccgct gggcggctgc ctgccgatcc tggtccagat gccggtcttc ctctccctct actgggtact cctggaaagc gtggaaatgc gccaggcgcc gtggctcggc tggatcaccg acctgtcggt gaaggatccg ttcttcatcc tgccgatcgt catgggcggc accatgctga tccagcagat gctcaacccg accccgccag acccgatgca ggccaaggtg atgaaactga tgccgatcat cttcaccttc ttcttcctct ggttcccagc cggtctggtg ctgtactggg tagtcaacaa ctgcctgtcg atcgcacagc agtggtacat tacccgcaag atcgaggcag ccgcgaagac cgcctga

Details for PsaeA.18877.a.B2.GE38450

HARVESTED ON:	8/29/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMW NKDYGQPELP AASASMNTTQ GLPDTPSASG TSSDVPTAQS SAAGSEAADK PVAVSDKLIQ VKTDVLDLAI DPRGGDIVQL GLLQYPRRLD RPDVPFPLFD NGRERTYLAQ SGLTGADGPD ASSAGRPLFR SAQSSYQLAD GQNELVVDLS FSHDGVNYIK RFTFHRGLKA DCSDKEKAQK KIECINENAY QVGVSYLIDN QSGKTWSGNL FAQLKRDGSA DPSSTTATGV STYLGAAVWT PDSPYKKIST KDMDKEQFKE SVQGGWVAWL QHYFVTAWVP TKGEQHQVMT RKDGQGNYIV GFTGPTLSVP AGSKVETDLT LYAGPKLQKH LKELSPG

Validated NT Sequence

atcttactca cttagcccgg ggagagttcc ttcaggtgct tctggagttt cgggccggca tacagggtca ggtcggtttc caccttgcta cccgccggaa cgctcagggt cgggccggtg aaaccgacga tgtagttgcc ctggccgtcc ttgcgggtca tgacctggtg ctgctcgccc ttggtcggaa cccaggcggt cacgaagtag tgttgcagcc aggcgaccca gccaccctgc acggactcct tgaactgttc cttgtccatg tccttggtgg agatcttctt gtacggcgaa tccggggtcc agaccgcggc gccgaggtag gtggacaccc cggtggcggt ggtcgaggac gggtcggcgc tgccgtcgcg cttgagctgg gcgaacaggt tgccgctcca ggtcttgccg ctctggttgt cgatcaggta gctgacgccg acctgatagg cgttttcatt gatgcactcg atcttcttct gggccttttc cttgtccgag caatcggctt tcaggccacg gtggaaggtg aagcgcttga tgtagttgac accgtcgtgg ctgaagctca ggtccacgac cagttcgttc tggccgtcgg ccaactggta gctgctctgc gcactgcgga acagtgggcg gccggcgctg ctggcgtccg ggccgtcggc accggtgaga ccgctctgcg ccaggtaggt acgttcgcga ccgttgtcga acagcgggaa cggcacgtcc ggacggtcca ggcggcgcgg atactgcaac aggccgagct ggacgatatc gccgccacgg ggatcgatgg ccaggtcgag gacgtcggtc ttcacctgga tcagcttgtc gctgaccgca accggcttgt cggcggcttc gctgccggcc gcgctgctct gggcagtggg aacgtcactg ctggtgcccg aggcggacgg agtgtccggc aggccttgcg tggtgttcat cgaagcgctg gcggcgggca gctcaggctg accgtaatcc ttgttccaca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHWN KDYGQPELPA ASASMNTTQG LPDTPSASGT SSDVPTAQSS AAGSEAADKP VAVSDKLIQV KTDVLDLAID PRGGDIVQLG LLQYPRRLDR PDVPFPLFDN GRERTYLAQS GLTGADGPDA SSAGRPLFRS AQSSYQLADG QNELVVDLSF SHDGVNYIKR FTFHRGLKAD CSDKEKAQKK IECINENAYQ VGVSYLIDNQ SGKTWSGNLF AQLKRDGSAD PSSTTATGVS TYLGAAVWTP DSPYKKISTK DMDKEQFKES VQGGWVAWLQ HYFVTAWVPT KGEQHQVMTR KDGQGNYIVG FTGPTLSVPA GSKVETDLTL YAGPKLQKHL KELSPG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattggaaca aggattacgg tcagcctgag ctgcccgccg ccagcgcttc gatgaacacc acgcaaggcc tgccggacac tccgtccgcc tcgggcacca gcagtgacgt tcccactgcc cagagcagcg cggccggcag cgaagccgcc gacaagccgg ttgcggtcag cgacaagctg atccaggtga agaccgacgt cctcgacctg gccatcgatc cccgtggcgg cgatatcgtc cagctcggcc tgttgcagta tccgcgccgc ctggaccgtc cggacgtgcc gttcccgctg ttcgacaacg gtcgcgaacg tacctacctg gcgcagagcg gtctcaccgg tgccgacggc ccggacgcca gcagcgccgg ccgcccactg ttccgcagtg cgcagagcag ctaccagttg gccgacggcc agaacgaact ggtcgtggac ctgagcttca gccacgacgg tgtcaactac atcaagcgct tcaccttcca ccgtggcctg aaagccgatt gctcggacaa ggaaaaggcc cagaagaaga tcgagtgcat caatgaaaac gcctatcagg tcggcgtcag ctacctgatc gacaaccaga gcggcaagac ctggagcggc aacctgttcg cccagctcaa gcgcgacggc agcgccgacc cgtcctcgac caccgccacc ggggtgtcca cctacctcgg cgccgcggtc tggaccccgg attcgccgta caagaagatc tccaccaagg acatggacaa ggaacagttc aaggagtccg tgcagggtgg ctgggtcgcc tggctgcaac actacttcgt gaccgcctgg gttccgacca agggcgagca gcaccaggtc atgacccgca aggacggcca gggcaactac atcgtcggtt tcaccggccc gaccctgagc gttccggcgg gtagcaaggt ggaaaccgac ctgaccctgt atgccggccc gaaactccag aagcacctga aggaactctc cccgggctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for PsaeA.18877.a.B2.PW37583

PURIFICATION DATe:	10/27/2014
CONCENTRATION:	9.3mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	20
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTGGAACA AGGATTACGG TCAGCCTGAG CTGCCCGCCG CCAGCGCTTC GATGAACACC ACGCAAGGCC TGCCGGACAC TCCGTCCGCC TCGGGCACCA GCAGTGACGT TCCCACTGCC CAGAGCAGCG CGGCCGGCAG CGAAGCCGCC GACAAGCCGG TTGCGGTCAG CGACAAGCTG ATCCAGGTGA AGACCGACGT CCTCGACCTG GCCATCGATC CCCGTGGCGG CGATATCGTC CAGCTCGGCC TGTTGCAGTA TCCGCGCCGC CTGGACCGTC CGGACGTGCC GTTCCCGCTG TTCGACAACG GTCGCGAACG TACCTACCTG GCGCAGAGCG GTCTCACCGG TGCCGACGGC CCGGACGCCA GCAGCGCCGG CCGCCCACTG TTCCGCAGTG CGCAGAGCAG CTACCAGTTG GCCGACGGCC AGAACGAACT GGTCGTGGAC CTGAGCTTCA GCCACGACGG TGTCAACTAC ATCAAGCGCT TCACCTTCCA CCGTGGCCTG AAAGCCGATT GCTCGGACAA GGAAAAGGCC CAGAAGAAGA TCGAGTGCAT CAATGAAAAC GCCTATCAGG TCGGCGTCAG CTACCTGATC GACAACCAGA GCGGCAAGAC CTGGAGCGGC AACCTGTTCG CCCAGCTCAA GCGCGACGGC AGCGCCGACC CGTCCTCGAC CACCGCCACC GGGGTGTCCA CCTACCTCGG CGCCGCGGTC TGGACCCCGG ATTCGCCGTA CAAGAAGATC TCCACCAAGG ACATGGACAA GGAACAGTTC AAGGAGTCCG TGCAGGGTGG CTGGGTCGCC TGGCTGCAAC ACTACTTCGT GACCGCCTGG GTTCCGACCA AGGGCGAGCA GCACCAGGTC ATGACCCGCA AGGACGGCCA GGGCAACTAC ATCGTCGGTT TCACCGGCCC GACCCTGAGC GTTCCGGCGG GTAGCAAGGT GGAAACCGAC CTGACCCTGT ATGCCGGCCC GAAACTCCAG AAGCACCTGA AGGAACTCTC CCCGGGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT