PsaeA.18873.a

Diaminopimelate epimerase (DAP epimerase) (EC 5.1.1.7)

CENTER ID: PsaeA.18873.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18873.a.B1.GE38447 full length 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5532
PGAT: pseudomonas:PA5278
RefSeq: NP_253965.1
UniProt: Q51564

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLRFTKMHG LGNDFMVLDL VSQHAHVQPK HVKLWGDRNT GIGFDQLLIV EAPSSPDVDF RYRIFNADGS EVEQCGNGAR CFARFVQDKR LTVKKSIRVE TKGGIIELNI RPDGQVTVDM GPPRLAPAEI PFQAEREALS YEIEVNGQRV ELAAVSMGNP HGVLRVENVD SAPVHSLGPQ LEVHPRFPKK ANIGFLQVLD PHHARLRVWE RGVGETQACG TGACAAAVAG IRQGWLQSPV QIDLPGGRLH IEWAGPGQPV MMTGPAVRVY EGQVRL
NT Sequence
ATGCTTTTGC GCTTCACCAA GATGCATGGC CTGGGCAACG ACTTCATGGT CCTCGACCTG GTCAGCCAGC ACGCCCATGT GCAGCCCAAG CACGTCAAGC TCTGGGGCGA CCGCAACACC GGAATCGGCT TCGACCAGTT GCTGATCGTC GAAGCCCCGA GCAGCCCCGA CGTCGACTTC CGCTACCGGA TCTTCAACGC CGACGGCTCC GAGGTCGAGC AGTGCGGCAA CGGCGCTCGC TGCTTCGCCC GCTTCGTTCA GGACAAGCGC CTGACGGTGA AGAAGAGCAT CCGCGTAGAA ACCAAGGGCG GCATCATCGA GCTGAATATC CGCCCGGACG GCCAGGTCAC GGTGGACATG GGTCCGCCGC GCCTGGCCCC GGCCGAGATA CCGTTCCAGG CCGAGCGCGA AGCCTTGTCC TATGAGATAG AGGTGAATGG CCAGCGCGTC GAACTGGCCG CGGTATCCAT GGGCAATCCC CATGGCGTGC TGCGCGTCGA GAACGTCGAC AGCGCACCGG TACACAGCCT CGGTCCGCAA CTGGAAGTCC ATCCGCGCTT CCCGAAGAAG GCCAACATCG GCTTCCTCCA GGTGCTCGAT CCGCACCATG CGCGCCTGCG CGTCTGGGAG CGCGGCGTCG GCGAGACCCA AGCCTGCGGC ACCGGCGCCT GCGCCGCGGC GGTTGCCGGG ATTCGCCAGG GCTGGCTGCA ATCGCCGGTG CAGATCGACC TCCCCGGCGG CCGCCTGCAC ATCGAGTGGG CAGGTCCCGG CCAGCCCGTT ATGATGACCG GGCCTGCCGT CCGCGTGTAT GAAGGCCAGG TCCGCCTGTA A
Details for PsaeA.18873.a.B1.GE38447
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML LRFTKMHGLG NDFMVLDLVS QHAHVQPKHV KLWGDRNTGI GFDQLLIVEA PSSPDVDFRY RIFNADGSEV EQCGNGARCF ARFVQDKRLT VKKSIRVETK GGIIELNIRP DGQVTVDMGP PRLAPAEIPF QAEREALSYE IEVNGQRVEL AAVSMGNPHG VLRVENVDSA PVHSLGPQLE VHPRFPKKAN IGFLQVLDPH HARLRVWERG VGETQACGTG ACAAAVAGIR QGWLQSPVQI DLPRRPPAHR VGRSRPARYD DRACRPXXXG QVRL
Validated NT Sequence
ttactcactt acaggcggac ctggccnnnn nnnngcggac ggcaggcccg gtcatcataa cgggctggcc gggacctgcc cactcgatgt gcaggcggcc gccgggggag gtcgatctgc accggcgatt gcagccagcc ctggcgaatc ccggcaaccg ccgcggcgca ggcgccggtg ccgcaggctt gggtctcgcc gacgccgcgc tcccagacgc gcaggcgcgc atggtgcgga tcgagcacct ggaggaagcc gatgttggcc ttcttcggga agcgcggatg gacttccagt tgcggaccga ggctgtgtac cggtgcgctg tcgacgttct cgacgcgcag cacgccatgg ggattgccca tggataccgc ggccagttcg acgcgctggc cattcacctc tatctcatag gacaaggctt cgcgctcggc ctggaacggt atctcggccg gggccaggcg cggcggaccc atgtccaccg tgacctggcc gtccgggcgg atattcagct cgatgatgcc gcccttggtt tctacgcgga tgctcttctt caccgtcagg cgcttgtcct gaacgaagcg ggcgaagcag cgagcgccgt tgccgcactg ctcgacctcg gagccgtcgg cgttgaagat ccggtagcgg aagtcgacgt cggggctgct cggggcttcg acgatcagca actggtcgaa gccgattccg gtgttgcggt cgccccagag cttgacgtgc ttgggctgca catgggcgtg ctggctgacc aggtcgagga ccatgaagtc gttgcccagg ccatgcatct tggtgaagcg caaaagcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHML LRFTKMHGLG NDFMVLDLVS QHAHVQPKHV KLWGDRNTGI GFDQLLIVEA PSSPDVDFRY RIFNADGSEV EQCGNGARCF ARFVQDKRLT VKKSIRVETK GGIIELNIRP DGQVTVDMGP PRLAPAEIPF QAEREALSYE IEVNGQRVEL AAVSMGNPHG VLRVENVDSA PVHSLGPQLE VHPRFPKKAN IGFLQVLDPH HARLRVWERG VGETQACGTG ACAAAVAGIR QGWLQSPVQI DLPGGRLHIE WAGPGQPVMM TGPAVRVYEG QVRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttt tgcgcttcac caagatgcat ggcctgggca acgacttcat ggtcctcgac ctggtcagcc agcacgccca tgtgcagccc aagcacgtca agctctgggg cgaccgcaac accggaatcg gcttcgacca gttgctgatc gtcgaagccc cgagcagccc cgacgtcgac ttccgctacc ggatcttcaa cgccgacggc tccgaggtcg agcagtgcgg caacggcgct cgctgcttcg cccgcttcgt tcaggacaag cgcctgacgg tgaagaagag catccgcgta gaaaccaagg gcggcatcat cgagctgaat atccgcccgg acggccaggt cacggtggac atgggtccgc cgcgcctggc cccggccgag ataccgttcc aggccgagcg cgaagccttg tcctatgaga tagaggtgaa tggccagcgc gtcgaactgg ccgcggtatc catgggcaat ccccatggcg tgctgcgcgt cgagaacgtc gacagcgcac cggtacacag cctcggtccg caactggaag tccatccgcg cttcccgaag aaggccaaca tcggcttcct ccaggtgctc gatccgcacc atgcgcgcct gcgcgtctgg gagcgcggcg tcggcgagac ccaagcctgc ggcaccggcg cctgcgccgc ggcggttgcc gggattcgcc agggctggct gcaatcgccg gtgcagatcg acctccccgg cggccgcctg cacatcgagt gggcaggtcc cggccagccc gttatgatga ccgggcctgc cgtccgcgtg tatgaaggcc aggtccgcct gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat