PsaeA.18861.a

Protein translocase subunit SecD

CENTER ID: PsaeA.18861.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18861.a.B2.GE38441 mature protein 25 461
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18861.a.B2.PW37581 mature protein 25 461

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4000
PGAT: pseudomonas:PA3821
RefSeq: NP_252510.1
UniProt: Q9HXI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNKYPLWKY LLILAVLAVG FIYSAPNLYP DDPAVQISGA STALQVTQAD VDRAAKALTD AGIAVKADSL SKKGGLIRLV KQDDQLPAKE VVRRTLGDDY VVALNLAQTT PEWLRKLGGS PMKLGLDLSG GVHFLLEVDM DKAVDARLKV YESEVKSLLR KERVRYRSLP IQDRAIQLGF TDSESLDKAR SLIAKDFRDF EVVPEERNGL QVLRVALTQA KLAEIREYSI KQNLTTVRNR VNELGVSEPL VQRQGANRIV VELPGVQDTA EAKRILGKTA NLEFRLAAEP DALKSATETF EFREPRRPPV PLERGVIITG DQVTDASASF DENGRPQVNI RLDGHGGELM NRATRNNVGR SMAVVFIEQK PVTRYTKQMV DGVEKEVAVP AFKEEKQIIS LATIQSPLGN QFRITGLDGP GESSELALLL RAGGLAAPMY FAEERTIGPS LGADNIAKGI DASLWGMLFV SLFIIVIYRF FGVIATVALA FNMVMLVALM SILGATLTLP GIAGIVLTMG MAVDANVLIF SRIREELANG MSVQRAIHEG FNRAFTAILD ANLTSLLVGG ILYAMGTGPV KGFAVTMSLG IITSMFTAIM VTRAMVNLIF GGRDFKKLWI
NT Sequence
atgctcaaca agtatcctct gtggaaatat ctgctgatcc tggcggtcct cgccgtcggg ttcatctatt ccgcacccaa cctctacccg gacgacccgg cggtgcagat cagcggcgcc agcacggcgc tgcaggtcac ccaggccgat gtcgaccgcg ctgccaaggc gctgaccgac gccggtatcg cggtgaaggc cgacagcctg tcgaagaagg gcggcctgat ccgcctggtc aagcaggacg accagcttcc cgccaaggaa gtggtgcgcc ggaccctggg cgacgactac gtggtggcgc tgaacctggc ccagaccact cccgagtggc tgcgcaagct gggcggcagc ccgatgaagc tcggcctgga tctgtccggc ggtgtgcact tcctgctgga agtggacatg gacaaggcgg tcgatgcccg cctgaaggtc tatgaaagcg aagtgaagag cctgctgcgc aaggagcgcg tgcgctaccg cagcctgccg atccaggatc gcgcgatcca gctcggtttc accgacagcg aatccctgga caaggcgcgc agcctgatcg ccaaggactt ccgcgatttc gaggtcgtgc ccgaagagcg caacggcctg caggtcctgc gcgtcgccct gacccaggcc aaactggcgg aaatccgcga atactccatc aagcagaacc tcaccaccgt ccgcaaccgg gtcaacgagc tgggcgtatc cgagccgctg gtgcagcgcc agggcgccaa tcgcatcgtg gtcgagctgc cgggcgtgca ggacaccgcg gaagccaagc ggatcctcgg caagaccgcc aacctagaat tccgcctggc cgccgagccg gacgccctga agtccgccac cgagaccttc gagttccgcg agccgcgccg gccgccggtg ccgctggagc gcggggtgat catcaccggc gaccaggtca ccgacgccag cgccagtttc gacgagaacg gccgcccgca ggtgaacatc cgcctggatg gccatggcgg cgagctgatg aaccgcgcca cccgcaacaa cgtcggccgc agcatggcgg tggtgttcat cgagcagaag ccggtgaccc gctacaccaa gcagatggtc gatggcgtcg agaaggaggt cgcggttccg gcgttcaagg aagagaagca gatcatcagc ctggcgacca tccagtcgcc gctgggcaac cagttccgta tcaccggcct cgatggcccg ggcgagtcct ccgagctggc cctgctgctg cgtgccggcg gcctggctgc gccgatgtac ttcgccgaag aacgcaccat cggcccgagc ctgggggcgg acaacatcgc caagggtatc gatgcgtcgc tctggggcat gctcttcgta tcgctgttca tcatcgtcat ctaccgtttc ttcggcgtga tcgccaccgt tgcgctggcc ttcaacatgg tcatgctggt ggcgctgatg tcgatcctcg gcgcgaccct gaccctgccg ggcatcgccg gtatcgtgct gaccatgggc atggcggtgg acgccaacgt gctgatcttc tcgcggatac gcgaggaact ggccaatggc atgtcggtcc agagggcgat ccacgaaggc ttcaaccgtg ccttcaccgc gatcctcgac gccaacctga cctcgctgct ggtcggcggc atcctctacg ccatgggcac cggcccggtg aaaggatttg ccgtgaccat gtcgctcggc atcatcacct cgatgttcac ggccatcatg gtcacccgcg caatggtcaa cctgatcttc ggcgggcgtg acttcaagaa gctgtggatc taa
Details for PsaeA.18861.a.B2.GE38441
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA PNLYPDDPAV QISGASTALQ VTQADVDRAA KALTDAGIAV KADSLSKKGG LIRLVKQDDQ LPAKEVVRRT LGDDYVVALN LAQTTPEWLR KLGGSPMKLG LDLSGGVHFL LEVDMDKAVD ARLKVYESEV KSLLRKERVR YRSLPIQDRA IQLGFTDSES LDKARSLIAK DFRDFEVVPE ERNGLQVLRV ALTQAKLAEI REYSIKQNLT TVRNRVNELG VSEPLVQRQG ANRIVVELPG VQDTAEAKRI LGKTANLEFR LAAEPDALKS ATETFEFREP RRPPVPLERG VIITGDQVTD ASASFDENGR PQVNIRLDGH GGELMNRATR NNVGRSMAVV FIEQKPVTRY TKQMVDGVEK EVAVPAFKEE KQIISLATIQ SPLGNQFRIT GLDGPGESSE LALLLRAGGL AAPMYFAEER TIGPSLGADN IAKGID
Validated NT Sequence
atcttactca ctaatcgata cccttggcga tgttgtccgc ccccaggctc gggccgatgg tgcgttcttc ggcgaagtac atcggcgcag ccaggccgcc ggcacgcagc agcagggcca gctcggagga ctcgcccggg ccatcgaggc cggtgatacg gaactggttg cccagcggcg actggatggt cgccaggctg atgatctgct tctcttcctt gaacgccgga accgcgacct ccttctcgac gccatcgacc atctgcttgg tgtagcgggt caccggcttc tgctcgatga acaccaccgc catgctgcgg ccgacgttgt tgcgggtggc gcggttcatc agctcgccgc catggccatc caggcggatg ttcacctgcg ggcggccgtt ctcgtcgaaa ctggcgctgg cgtcggtgac ctggtcgccg gtgatgatca ccccgcgctc cagcggcacc ggcggccggc gcggctcgcg gaactcgaag gtctcggtgg cggacttcag ggcgtccggc tcggcggcca ggcggaattc taggttggcg gtcttgccga ggatccgctt ggcttccgcg gtgtcctgca cgcccggcag ctcgaccacg atgcgattgg cgccctggcg ctgcaccagc ggctcggata cgcccagctc gttgacccgg ttgcggacgg tggtgaggtt ctgcttgatg gagtattcgc ggatttccgc cagtttggcc tgggtcaggg cgacgcgcag gacctgcagg ccgttgcgct cttcgggcac gacctcgaaa tcgcggaagt ccttggcgat caggctgcgc gccttgtcca gggattcgct gtcggtgaaa ccgagctgga tcgcgcgatc ctggatcggc aggctgcggt agcgcacgcg ctccttgcgc agcaggctct tcacttcgct ttcatagacc ttcaggcggg catcgaccgc cttgtccatg tccacttcca gcaggaagtg cacaccgccg gacagatcca ggccgagctt catcgggctg ccgcccagct tgcgcagcca ctcgggagtg gtctgggcca ggttcagcgc caccacgtag tcgtcgccca gggtccggcg caccacttcc ttggcgggaa gctggtcgtc ctgcttgacc aggcggatca ggccgccctt cttcgacagg ctgtcggcct tcaccgcgat accggcgtcg gtcagcgcct tggcagcgcg gtcgacatcg gcctgggtga cctgcagcgc cgtgctggcg ccgctgatct gcaccgccgg gtcgtccggg tagaggttgg gtgccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHAP NLYPDDPAVQ ISGASTALQV TQADVDRAAK ALTDAGIAVK ADSLSKKGGL IRLVKQDDQL PAKEVVRRTL GDDYVVALNL AQTTPEWLRK LGGSPMKLGL DLSGGVHFLL EVDMDKAVDA RLKVYESEVK SLLRKERVRY RSLPIQDRAI QLGFTDSESL DKARSLIAKD FRDFEVVPEE RNGLQVLRVA LTQAKLAEIR EYSIKQNLTT VRNRVNELGV SEPLVQRQGA NRIVVELPGV QDTAEAKRIL GKTANLEFRL AAEPDALKSA TETFEFREPR RPPVPLERGV IITGDQVTDA SASFDENGRP QVNIRLDGHG GELMNRATRN NVGRSMAVVF IEQKPVTRYT KQMVDGVEKE VAVPAFKEEK QIISLATIQS PLGNQFRITG LDGPGESSEL ALLLRAGGLA APMYFAEERT IGPSLGADNI AKGID
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaccca acctctaccc ggacgacccg gcggtgcaga tcagcggcgc cagcacggcg ctgcaggtca cccaggccga tgtcgaccgc gctgccaagg cgctgaccga cgccggtatc gcggtgaagg ccgacagcct gtcgaagaag ggcggcctga tccgcctggt caagcaggac gaccagcttc ccgccaagga agtggtgcgc cggaccctgg gcgacgacta cgtggtggcg ctgaacctgg cccagaccac tcccgagtgg ctgcgcaagc tgggcggcag cccgatgaag ctcggcctgg atctgtccgg cggtgtgcac ttcctgctgg aagtggacat ggacaaggcg gtcgatgccc gcctgaaggt ctatgaaagc gaagtgaaga gcctgctgcg caaggagcgc gtgcgctacc gcagcctgcc gatccaggat cgcgcgatcc agctcggttt caccgacagc gaatccctgg acaaggcgcg cagcctgatc gccaaggact tccgcgattt cgaggtcgtg cccgaagagc gcaacggcct gcaggtcctg cgcgtcgccc tgacccaggc caaactggcg gaaatccgcg aatactccat caagcagaac ctcaccaccg tccgcaaccg ggtcaacgag ctgggcgtat ccgagccgct ggtgcagcgc cagggcgcca atcgcatcgt ggtcgagctg ccgggcgtgc aggacaccgc ggaagccaag cggatcctcg gcaagaccgc caacctagaa ttccgcctgg ccgccgagcc ggacgccctg aagtccgcca ccgagacctt cgagttccgc gagccgcgcc ggccgccggt gccgctggag cgcggggtga tcatcaccgg cgaccaggtc accgacgcca gcgccagttt cgacgagaac ggccgcccgc aggtgaacat ccgcctggat ggccatggcg gcgagctgat gaaccgcgcc acccgcaaca acgtcggccg cagcatggcg gtggtgttca tcgagcagaa gccggtgacc cgctacacca agcagatggt cgatggcgtc gagaaggagg tcgcggttcc ggcgttcaag gaagagaagc agatcatcag cctggcgacc atccagtcgc cgctgggcaa ccagttccgt atcaccggcc tcgatggccc gggcgagtcc tccgagctgg ccctgctgct gcgtgccggc ggcctggctg cgccgatgta cttcgccgaa gaacgcacca tcggcccgag cctgggggcg gacaacatcg ccaagggtat cgattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.18861.a.B2.PW37581
PURIFICATION DATe: 10/27/2014
CONCENTRATION: 20mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA PNLYPDDPAV QISGASTALQ VTQADVDRAA KALTDAGIAV KADSLSKKGG LIRLVKQDDQ LPAKEVVRRT LGDDYVVALN LAQTTPEWLR KLGGSPMKLG LDLSGGVHFL LEVDMDKAVD ARLKVYESEV KSLLRKERVR YRSLPIQDRA IQLGFTDSES LDKARSLIAK DFRDFEVVPE ERNGLQVLRV ALTQAKLAEI REYSIKQNLT TVRNRVNELG VSEPLVQRQG ANRIVVELPG VQDTAEAKRI LGKTANLEFR LAAEPDALKS ATETFEFREP RRPPVPLERG VIITGDQVTD ASASFDENGR PQVNIRLDGH GGELMNRATR NNVGRSMAVV FIEQKPVTRY TKQMVDGVEK EVAVPAFKEE KQIISLATIQ SPLGNQFRIT GLDGPGESSE LALLLRAGGL AAPMYFAEER TIGPSLGADN IAKGID
Validated NT Sequence
atcttactca ctaatcgata cccttggcga tgttgtccgc ccccaggctc gggccgatgg tgcgttcttc ggcgaagtac atcggcgcag ccaggccgcc ggcacgcagc agcagggcca gctcggagga ctcgcccggg ccatcgaggc cggtgatacg gaactggttg cccagcggcg actggatggt cgccaggctg atgatctgct tctcttcctt gaacgccgga accgcgacct ccttctcgac gccatcgacc atctgcttgg tgtagcgggt caccggcttc tgctcgatga acaccaccgc catgctgcgg ccgacgttgt tgcgggtggc gcggttcatc agctcgccgc catggccatc caggcggatg ttcacctgcg ggcggccgtt ctcgtcgaaa ctggcgctgg cgtcggtgac ctggtcgccg gtgatgatca ccccgcgctc cagcggcacc ggcggccggc gcggctcgcg gaactcgaag gtctcggtgg cggacttcag ggcgtccggc tcggcggcca ggcggaattc taggttggcg gtcttgccga ggatccgctt ggcttccgcg gtgtcctgca cgcccggcag ctcgaccacg atgcgattgg cgccctggcg ctgcaccagc ggctcggata cgcccagctc gttgacccgg ttgcggacgg tggtgaggtt ctgcttgatg gagtattcgc ggatttccgc cagtttggcc tgggtcaggg cgacgcgcag gacctgcagg ccgttgcgct cttcgggcac gacctcgaaa tcgcggaagt ccttggcgat caggctgcgc gccttgtcca gggattcgct gtcggtgaaa ccgagctgga tcgcgcgatc ctggatcggc aggctgcggt agcgcacgcg ctccttgcgc agcaggctct tcacttcgct ttcatagacc ttcaggcggg catcgaccgc cttgtccatg tccacttcca gcaggaagtg cacaccgccg gacagatcca ggccgagctt catcgggctg ccgcccagct tgcgcagcca ctcgggagtg gtctgggcca ggttcagcgc caccacgtag tcgtcgccca gggtccggcg caccacttcc ttggcgggaa gctggtcgtc ctgcttgacc aggcggatca ggccgccctt cttcgacagg ctgtcggcct tcaccgcgat accggcgtcg gtcagcgcct tggcagcgcg gtcgacatcg gcctgggtga cctgcagcgc cgtgctggcg ccgctgatct gcaccgccgg gtcgtccggg tagaggttgg gtgccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHAP NLYPDDPAVQ ISGASTALQV TQADVDRAAK ALTDAGIAVK ADSLSKKGGL IRLVKQDDQL PAKEVVRRTL GDDYVVALNL AQTTPEWLRK LGGSPMKLGL DLSGGVHFLL EVDMDKAVDA RLKVYESEVK SLLRKERVRY RSLPIQDRAI QLGFTDSESL DKARSLIAKD FRDFEVVPEE RNGLQVLRVA LTQAKLAEIR EYSIKQNLTT VRNRVNELGV SEPLVQRQGA NRIVVELPGV QDTAEAKRIL GKTANLEFRL AAEPDALKSA TETFEFREPR RPPVPLERGV IITGDQVTDA SASFDENGRP QVNIRLDGHG GELMNRATRN NVGRSMAVVF IEQKPVTRYT KQMVDGVEKE VAVPAFKEEK QIISLATIQS PLGNQFRITG LDGPGESSEL ALLLRAGGLA APMYFAEERT IGPSLGADNI AKGID
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCACCCA ACCTCTACCC GGACGACCCG GCGGTGCAGA TCAGCGGCGC CAGCACGGCG CTGCAGGTCA CCCAGGCCGA TGTCGACCGC GCTGCCAAGG CGCTGACCGA CGCCGGTATC GCGGTGAAGG CCGACAGCCT GTCGAAGAAG GGCGGCCTGA TCCGCCTGGT CAAGCAGGAC GACCAGCTTC CCGCCAAGGA AGTGGTGCGC CGGACCCTGG GCGACGACTA CGTGGTGGCG CTGAACCTGG CCCAGACCAC TCCCGAGTGG CTGCGCAAGC TGGGCGGCAG CCCGATGAAG CTCGGCCTGG ATCTGTCCGG CGGTGTGCAC TTCCTGCTGG AAGTGGACAT GGACAAGGCG GTCGATGCCC GCCTGAAGGT CTATGAAAGC GAAGTGAAGA GCCTGCTGCG CAAGGAGCGC GTGCGCTACC GCAGCCTGCC GATCCAGGAT CGCGCGATCC AGCTCGGTTT CACCGACAGC GAATCCCTGG ACAAGGCGCG CAGCCTGATC GCCAAGGACT TCCGCGATTT CGAGGTCGTG CCCGAAGAGC GCAACGGCCT GCAGGTCCTG CGCGTCGCCC TGACCCAGGC CAAACTGGCG GAAATCCGCG AATACTCCAT CAAGCAGAAC CTCACCACCG TCCGCAACCG GGTCAACGAG CTGGGCGTAT CCGAGCCGCT GGTGCAGCGC CAGGGCGCCA ATCGCATCGT GGTCGAGCTG CCGGGCGTGC AGGACACCGC GGAAGCCAAG CGGATCCTCG GCAAGACCGC CAACCTAGAA TTCCGCCTGG CCGCCGAGCC GGACGCCCTG AAGTCCGCCA CCGAGACCTT CGAGTTCCGC GAGCCGCGCC GGCCGCCGGT GCCGCTGGAG CGCGGGGTGA TCATCACCGG CGACCAGGTC ACCGACGCCA GCGCCAGTTT CGACGAGAAC GGCCGCCCGC AGGTGAACAT CCGCCTGGAT GGCCATGGCG GCGAGCTGAT GAACCGCGCC ACCCGCAACA ACGTCGGCCG CAGCATGGCG GTGGTGTTCA TCGAGCAGAA GCCGGTGACC CGCTACACCA AGCAGATGGT CGATGGCGTC GAGAAGGAGG TCGCGGTTCC GGCGTTCAAG GAAGAGAAGC AGATCATCAG CCTGGCGACC ATCCAGTCGC CGCTGGGCAA CCAGTTCCGT ATCACCGGCC TCGATGGCCC GGGCGAGTCC TCCGAGCTGG CCCTGCTGCT GCGTGCCGGC GGCCTGGCTG CGCCGATGTA CTTCGCCGAA GAACGCACCA TCGGCCCGAG CCTGGGGGCG GACAACATCG CCAAGGGTAT CGATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT