PsaeA.18859.a

Uncharacterized protein (HHPRED: serine/threonine kinase)

CENTER ID: PsaeA.18859.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18859.a.B1.GE38440 full length 1 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5246
PGAT: pseudomonas:PA5006
RefSeq: NP_253693.1
UniProt: Q9HUF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLAELQAAG RQPQLPLRVT LADGAGAAEL RLDSLLRVLP GQRYVGAGEW RGRKVLAKLL VGGKAARHFQ RELEGARLLA AQGLTTPALL ADGLQQGEGG WLLFDYLDAA QSLGDAWRAV EAQAPLGDAQ QAVLGEALAA IGELHAKGLW QDDLHLDNLL RHAGRLYLID GGGVRAETPG QPLSRQKVLE NLGVFFAQLP ALLDPFLEEL LVHYLLANGE HALPLEALQK EIAKVRRWRV RDLLRKVGRD CSLFSVVRGP FRLFAARRSE AETMAPLLAD ADRLIAAGHV YKDGGAATVA RVEANGRTLI IKRYNIKGWV HWLKRFWRPS RAWASWREGN RLEFLGIATA RPLAMLERRW CWLRGRAYLI TDYLGGQDII ARFKPCLDMP DGAVSPPEAE LAALDRLFST LIRERISHGD LKGHNLFWQA HEQGGEWALI DLDAMRKHHS QASFARAYAR DRARFLRNWP QDSGLYRLLE QRIPRLGDAA GA
NT Sequence
atgagactgg ccgaactgca agccgccggg cgccagccgc aactgccatt gcgggtgacc ctggcggatg gcgccggcgc tgccgaactg cgcctggaca gtctgctgcg ggtgctgccc gggcagcgct acgtcggtgc cggcgaatgg cgtgggcgca aggtgctggc caagctgctg gtgggcggca aggccgcacg gcatttccag cgcgaactgg aaggggcgcg tctgctcgcg gcccaggggc tgaccacccc ggcgctgctg gccgacgggc tgcagcaggg cgagggcggc tggttgctgt tcgactacct cgacgcggcg cagagcctcg gtgacgcctg gcgtgcggtg gaggcccagg cgccgctggg cgatgcccag caagcggtgc tcggcgaagc cctggcagcc attggcgagc tgcatgcaaa gggcctctgg caggacgacc tgcacctgga caacctgttg cgccacgccg gccgcctgta tctcatcgac ggtggcggcg tgcgtgcgga aactcccggc cagccgctgt cgcgacagaa agtgctggaa aacctcgggg tgttcttcgc ccagttgccg gcgctcctcg atcctttcct ggaggagttg ctggtgcatt acctgctggc caacggcgag catgcgctgc cgctggaagc cttgcagaag gaaatcgcca aggttcgccg ctggcgggtg cgcgacctgc tgagaaaggt cggtcgcgac tgtagcctgt tcagcgtcgt gcgcggtccg ttccgcctgt tcgccgcccg ccgcagcgag gccgagacga tggcgcccct gctggcggac gcggaccgcc tgatcgccgc cggccacgtc tacaaggacg gcggcgcggc caccgtcgcc cgggtcgaag cgaacggccg cacgctgatc atcaagcgct acaacatcaa gggctgggtc cattggctga agcgcttctg gcggccgagc cgggcctggg cctcctggcg cgaaggcaac cgcctggaat tcctcggcat cgccaccgcc cgtccgctgg cgatgctgga gcggcgctgg tgctggctgc gcgggcgcgc ctacctgatt accgattatc tcggcggtca ggatataatc gctcgtttta agccttgcct ggacatgccc gatggcgccg tttcgccacc ggaagccgag ttggcggcac tggatcggct gttttcgacg ttgatccgcg aacgcatcag ccacggcgac ctgaagggac acaacctgtt ctggcaggcc catgaacaag gcggcgaatg ggcgctgatc gacctggacg cgatgcgcaa gcatcacagc caggcgtcct ttgcccgcgc ctacgcccgc gaccgcgcgc gcttcctgcg caactggccg caggacagcg gcctctaccg gctgctggag caacggattc cccgcctcgg cgacgccgcc ggggcttga
Details for PsaeA.18859.a.B1.GE38440
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 56kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR LAELQAAGRQ PQLPLRVTLA DGAGAAELRL DSLLRVLPGQ RYVGAGEWRG RKVLAKLLVG GKAARHFQRE LEGARLLAAQ GLTTPALLAD GLQQGEGGWL LFDYLDAAQS LGDAWRAVEA QAPLGDAQQA VLGEALAAIG ELHAKGLWQD DLHLDNLLRH AGRLYLIDGG GVRAETPGQP LSRQKVLENL GVFFAQLPAL LDPFLEELLV HYLLANGEHA LPLEALQKEI AKVRRWRVRD LLRKVGRDCX XFSVVRGPFR LFAARRSEAE TMAPLLADAD RLIAAGHVYK DGGAATVARV EANGRTLIIK RYNIKGWVHW LKRFWRPSRA WASWREGNRL EFLGIATARP LAMLERRWCW LRGRAYLITD YLGGQDIIAR FKPCLDMPDG AVSPPEAELA ALDRLFSTLI RERISHGDLK GHNLFWQAHE QGGEWALIDL DAMRKHHSQA SFARAYARDR ARFLRNWPQD SGLYRLLEQR IP
Validated NT Sequence
ggggaatccg ttgctccagc agccggtaga ggccgctgtc ctgcggccag ttgcgcagga agcgcgcgcg gtcgcgggcg taggcgcggg caaaggacgc ctggctgtga tgcttgcgca tcgcgtccag gtcgatcagc gcccattcgc cgccttgttc atgggcctgc cagaacaggt tgtgtccctt caggtcgccg tggctgatgc gttcgcggat caacgtcgaa aacagccgat ccagtgccgc caactcggct tccggtggcg aaacggcgcc atcgggcatg tccaggcaag gcttaaaacg agcgattata tcctgaccgc cgagataatc ggtaatcagg taggcgcgcc cgcgcagcca gcaccagcgc cgctccagca tcgccagcgg acgggcggtg gcgatgccga ggaattccag gcggttgcct tcgcgccagg aggcccaggc ccggctcggc cgccagaagc gcttcagcca atggacccag cccttgatgt tgtagcgctt gatgatcagc gtgcggccgt tcgcttcgac ccgggcgacg gtggccgcgc cgccgtcctt gtagacgtgg ccggcggcga tcaggcggtc cgcgtccgcc agcaggggcg ccatcgtctc ggcctcgctg cggcgggcgg cgaacaggcg gaacggaccg cgcacgacgc tgaacnnggn acagtcgcga ccgacctttc tcagcaggtc gcgcacccgc cagcggcgaa ccttggcgat ttccttctgc aaggcttcca gcggcagcgc atgctcgccg ttggccagca ggtaatgcac cagcaactcc tccaggaaag gatcgaggag cgccggcaac tgggcgaaga acaccccgag gttttccagc actttctgtc gcgacagcgg ctggccggga gtttccgcac gcacgccgcc accgtcgatg agatacaggc ggccggcgtg gcgcaacagg ttgtccaggt gcaggtcgtc ctgccagagg ccctttgcat gcagctcgcc aatggctgcc agggcttcgc cgagcaccgc ttgctgggca tcgcccagcg gcgcctgggc ctccaccgca cgccaggcgt caccgaggct ctgcgccgcg tcgaggtagt cgaacagcaa ccagccgccc tcgccctgct gcagcccgtc ggccagcagc gccggggtgg tcagcccctg ggccgcgagc agacgcgccc cttccagttc gcgctggaaa tgccgtgcgg ccttgccgcc caccagcagc ttggccagca ccttgcgccc acgccattcg ccggcaccga cgtagcgctg cccgggcagc acccgcagca gactgtccag gcgcagttcg gcagcgccgg cgccatccgc cagggtcacc cgcaatggca gttgcggctg gcgcccggcg gcttgcagtt cggccagtct catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMR LAELQAAGRQ PQLPLRVTLA DGAGAAELRL DSLLRVLPGQ RYVGAGEWRG RKVLAKLLVG GKAARHFQRE LEGARLLAAQ GLTTPALLAD GLQQGEGGWL LFDYLDAAQS LGDAWRAVEA QAPLGDAQQA VLGEALAAIG ELHAKGLWQD DLHLDNLLRH AGRLYLIDGG GVRAETPGQP LSRQKVLENL GVFFAQLPAL LDPFLEELLV HYLLANGEHA LPLEALQKEI AKVRRWRVRD LLRKVGRDCS LFSVVRGPFR LFAARRSEAE TMAPLLADAD RLIAAGHVYK DGGAATVARV EANGRTLIIK RYNIKGWVHW LKRFWRPSRA WASWREGNRL EFLGIATARP LAMLERRWCW LRGRAYLITD YLGGQDIIAR FKPCLDMPDG AVSPPEAELA ALDRLFSTLI RERISHGDLK GHNLFWQAHE QGGEWALIDL DAMRKHHSQA SFARAYARDR ARFLRNWPQD SGLYRLLEQR IPRLGDAAGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagac tggccgaact gcaagccgcc gggcgccagc cgcaactgcc attgcgggtg accctggcgg atggcgccgg cgctgccgaa ctgcgcctgg acagtctgct gcgggtgctg cccgggcagc gctacgtcgg tgccggcgaa tggcgtgggc gcaaggtgct ggccaagctg ctggtgggcg gcaaggccgc acggcatttc cagcgcgaac tggaaggggc gcgtctgctc gcggcccagg ggctgaccac cccggcgctg ctggccgacg ggctgcagca gggcgagggc ggctggttgc tgttcgacta cctcgacgcg gcgcagagcc tcggtgacgc ctggcgtgcg gtggaggccc aggcgccgct gggcgatgcc cagcaagcgg tgctcggcga agccctggca gccattggcg agctgcatgc aaagggcctc tggcaggacg acctgcacct ggacaacctg ttgcgccacg ccggccgcct gtatctcatc gacggtggcg gcgtgcgtgc ggaaactccc ggccagccgc tgtcgcgaca gaaagtgctg gaaaacctcg gggtgttctt cgcccagttg ccggcgctcc tcgatccttt cctggaggag ttgctggtgc attacctgct ggccaacggc gagcatgcgc tgccgctgga agccttgcag aaggaaatcg ccaaggttcg ccgctggcgg gtgcgcgacc tgctgagaaa ggtcggtcgc gactgtagcc tgttcagcgt cgtgcgcggt ccgttccgcc tgttcgccgc ccgccgcagc gaggccgaga cgatggcgcc cctgctggcg gacgcggacc gcctgatcgc cgccggccac gtctacaagg acggcggcgc ggccaccgtc gcccgggtcg aagcgaacgg ccgcacgctg atcatcaagc gctacaacat caagggctgg gtccattggc tgaagcgctt ctggcggccg agccgggcct gggcctcctg gcgcgaaggc aaccgcctgg aattcctcgg catcgccacc gcccgtccgc tggcgatgct ggagcggcgc tggtgctggc tgcgcgggcg cgcctacctg attaccgatt atctcggcgg tcaggatata atcgctcgtt ttaagccttg cctggacatg cccgatggcg ccgtttcgcc accggaagcc gagttggcgg cactggatcg gctgttttcg acgttgatcc gcgaacgcat cagccacggc gacctgaagg gacacaacct gttctggcag gcccatgaac aaggcggcga atgggcgctg atcgacctgg acgcgatgcg caagcatcac agccaggcgt cctttgcccg cgcctacgcc cgcgaccgcg cgcgcttcct gcgcaactgg ccgcaggaca gcggcctcta ccggctgctg gagcaacgga ttccccgcct cggcgacgcc gccggggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat