SSGCID: Target Page

PsaeA.18858.a

Signal peptidase I (SPase I) (EC 3.4.21.89) (Leader peptidase I)

CENTER ID:	PsaeA.18858.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18858.a.B2.GE40334	soluble domain		81	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.798
PGAT:	pseudomonas:PA0768
RefSeq:	NP_249459.1
UniProt:	Q9I5G7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLNFPLLLV IAVAVCGALA LVDLVLFAPR RRAAISSYEG QVNEPDPAVL EKLNKEPLLV EYGKSFFPVL FIVLVLRSFL VEPFQIPSGS MKPTLEVGDF ILVNKFAYGI RLPVLDTKVI PIGDPQRGDV MVFRYPSEPN INYIKRVVGL PGDTVRYTKE KRLYVNGELV AEKLVGEEPG TLGSVTLYQE KLGQAEHLIR KEMSRYRIEP DRQWTIPAGH YFMMGDNRDN SNDSRYWNDP KIPKDLLGMV PDRNIVGKAF AVWMSWPDPK MSNLPNFSRV GVIH

NT Sequence

atgacactca atttcccgtt gttgctggtc atcgccgtgg ctgtatgcgg cgccctggcc ctggtcgacc tggtgctgtt cgcgccgcgt cggcgggccg cgatctcttc ctacgaaggg caggtgaacg agcccgatcc ggcagtgctg gagaagctca acaaggaacc gctgctggtg gagtacggca agtcgttctt cccggtgctg ttcatcgtgc tggtgctgcg ttccttcctg gtcgagccgt tccagattcc ctcggggtcg atgaaaccta ccctcgaggt cggcgatttc atcctggtca acaagttcgc ctacggtatc cgcctgccgg tgctggacac caaggtgatc ccgatcggtg atccgcagcg cggcgatgtc atggtgttcc gctatcccag cgaaccgaac atcaactaca tcaagcgcgt ggtcggcctg cccggcgaca ccgtgcgcta caccaaggaa aagcgcctgt acgtcaacgg cgagctggtg gcggagaaac tggtcggcga ggaaccgggc accctgggca gcgtgaccct gtaccaggag aagctgggcc aggccgagca cctgatccgc aaggaaatga gccgctatcg catcgagccc gaccgccagt ggaccattcc cgccggccac tacttcatga tgggcgacaa ccgcgacaac tccaacgaca gccgctactg gaacgatccg aagatcccca aggatctgct gggcatggtt ccggaccgca atatcgtcgg caaggccttc gccgtgtgga tgagctggcc cgatccgaag atgagcaacc tgccgaactt ctcccgggtc ggcgtgattc actga

Details for PsaeA.18858.a.B2.GE40334

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV EPFQIPSGSM KPTLEVGDFI LVNKFAYGIR LPVLDTKVIP IGDPQRGDVM VFRYPSEPNI NYIKRVVGLP GDTVRYTKEK RLYVNGELVA EKLVGEEPGT LGSVTLYQEK LGQAEHLIRK EMSRYRIEPD RQWTIPAGHY FMMGDNRDNS NDSRYWNDPK IPKDLLGMVP DRNIVGKAFA VWMSWPDPKM SNLPNFSRVG VIH

Validated NT Sequence

atcttactca cttagtgaat cacgccgacc cgggagaagt tcggcaggtt gctcatcttc ggatcgggcc agctcatcca cacggcgaag gccttgccga cgatattgcg gtccggaacc atgcccagca gatccttggg gatcttcgga tcgttccagt agcggctgtc gttggagttg tcgcggttgt cgcccatcat gaagtagtgg ccggcgggaa tggtccactg gcggtcgggc tcgatgcgat agcggctcat ttccttgcgg atcaggtgct cggcctggcc cagcttctcc tggtacaggg tcacgctgcc cagggtgccc ggttcctcgc cgaccagttt ctccgccacc agctcgccgt tgacgtacag gcgcttttcc ttggtgtagc gcacggtgtc gccgggcagg ccgaccacgc gcttgatgta gttgatgttc ggttcgctgg gatagcggaa caccatgaca tcgccgcgct gcggatcacc gatcgggatc accttggtgt ccagcaccgg caggcggata ccgtaggcga acttgttgac caggatgaaa tcgccgacct cgagggtagg tttcatcgac cccgagggaa tctggaacgg ctcgaccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHVE PFQIPSGSMK PTLEVGDFIL VNKFAYGIRL PVLDTKVIPI GDPQRGDVMV FRYPSEPNIN YIKRVVGLPG DTVRYTKEKR LYVNGELVAE KLVGEEPGTL GSVTLYQEKL GQAEHLIRKE MSRYRIEPDR QWTIPAGHYF MMGDNRDNSN DSRYWNDPKI PKDLLGMVPD RNIVGKAFAV WMSWPDPKMS NLPNFSRVGV IH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtcgagc cgttccagat tccctcgggg tcgatgaaac ctaccctcga ggtcggcgat ttcatcctgg tcaacaagtt cgcctacggt atccgcctgc cggtgctgga caccaaggtg atcccgatcg gtgatccgca gcgcggcgat gtcatggtgt tccgctatcc cagcgaaccg aacatcaact acatcaagcg cgtggtcggc ctgcccggcg acaccgtgcg ctacaccaag gaaaagcgcc tgtacgtcaa cggcgagctg gtggcggaga aactggtcgg cgaggaaccg ggcaccctgg gcagcgtgac cctgtaccag gagaagctgg gccaggccga gcacctgatc cgcaaggaaa tgagccgcta tcgcatcgag cccgaccgcc agtggaccat tcccgccggc cactacttca tgatgggcga caaccgcgac aactccaacg acagccgcta ctggaacgat ccgaagatcc ccaaggatct gctgggcatg gttccggacc gcaatatcgt cggcaaggcc ttcgccgtgt ggatgagctg gcccgatccg aagatgagca acctgccgaa cttctcccgg gtcggcgtga ttcactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat