PsaeA.18855.a

Uncharacterized protein (HHPRED: no hit)

CENTER ID: PsaeA.18855.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18855.a.B2.GE38438 soluble domain 122 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4008
PGAT: pseudomonas:PA3828
RefSeq: NP_252517.1
UniProt: Q9HXH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIVFRYLSRE VLVTMSAVSA VLLVIIMSGR FIKYLAQAAQ GLLDPGSLFL IMAFRIPGFL QLILPLGLFL GILLAYGRLY LESEMTVLSA TGMSQKRLLG YTMAPALLVA ILVAWLSLFL APQGINQFAL LLNKQDTLTE FDTLVPGRFQ AMRDGTRVTY TEELSKDRGE LAGIFISQKD LNSSNQERGI SILVAEKGTQ NIQADGSRYL ILHNGYRYDG NPGQANYRAI QYDTYGVMLP KPEASSEVSE RDAVPTADLF GSDNPRYQAE LQWRLSTPLL VFVVTLLAVP LSRVNPRQGR FLKLLPAILL YMGYLALLIA VRGQLDKGKI PMAIGLWWVH GLFLAIGLLL FYWEPLRLKL ASSRAGREVA HG
NT Sequence
ttgatcgttt tccgttattt gtcccgtgaa gtgctggtca ccatgagcgc cgtcagcgcc gtgctgctgg tgatcatcat gagtggccgt ttcatcaagt acctggcgca agcggcgcag gggttgctcg accccggttc gctgttcctg atcatggcgt tccggattcc cggcttcctg cagctgatcc tgccgcttgg cctgttcctc ggcatcctgc tggcctacgg gcgactctat ctggaaagcg agatgaccgt gctctcggcg accggcatga gccagaagcg cctgctcggc tataccatgg ccccggcgct gctggtggcg atcctggtgg cctggctgag cctgttcctg gcgccccagg gcatcaacca gttcgccctg ctgttgaaca agcaggatac cctgaccgag ttcgacaccc tggtcccggg ccgtttccag gcaatgcgcg acggtacccg ggtgacctat accgaggaac tgtcgaagga tcgcggcgaa ctggccggca tcttcatttc gcagaaggac ctcaacagca gcaaccagga acgcggcatc tccatcctgg tggcggagaa gggcacccag aacatccagg cggatggcag ccgttacctg atcctgcaca acggctaccg ctacgacggc aatcccgggc aggccaacta ccgggcgatc cagtacgaca cctatggcgt gatgctgccc aagcccgagg ccagcagcga ggtgagcgag cgtgacgcgg tgcccaccgc cgacctgttc ggcagcgaca acccacgcta ccaggccgaa ttgcagtggc gcctgtcgac accgctgctg gtgttcgtgg tgaccctgct ggcggtgccg ctgtcgcggg tcaacccacg ccaggggcgc ttcctcaagc tgctgccggc gatcctcctt tatatgggct acctggccct gctgatcgct gtccgtggcc aactggacaa aggcaagatc cccatggcca tcggcctgtg gtgggtccac ggactattcc tggcgatcgg tcttcttctg ttctattggg aacccttgcg cctgaagctg gcgtcctccc gtgccggtcg cgaggtggcg catggttaa
Details for PsaeA.18855.a.B2.GE38438
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP QGINQFALLL NKQDTLTEFD TLVPGRFQAM RDGTRVTYTE ELSKDRGELA GIFISQKDLN SSNQERGISI LVAEKGTQNI QADGSRYLIL HNGYRYDGNP GQANYRAIQY DTYGVMLPKP EASSEVSERD AVPTADLFGS DNPRYQAE
Validated NT Sequence
atcttactca cttattcggc ctggtagcgt gggttgtcgc tgccgaacag gtcggcggtg ggcaccgcgt cacgctcgct cacctcgctg ctggcctcgg gcttgggcag catcacgcca taggtgtcgt actggatcgc ccggtagttg gcctgcccgg gattgccgtc gtagcggtag ccgttgtgca ggatcaggta acggctgcca tccgcctgga tgttctgggt gcccttctcc gccaccagga tggagatgcc gcgttcctgg ttgctgctgt tgaggtcctt ctgcgaaatg aagatgccgg ccagttcgcc gcgatccttc gacagttcct cggtataggt cacccgggta ccgtcgcgca ttgcctggaa acggcccggg accagggtgt cgaactcggt cagggtatcc tgcttgttca acagcagggc gaactggttg atgccctggg gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHPQ GINQFALLLN KQDTLTEFDT LVPGRFQAMR DGTRVTYTEE LSKDRGELAG IFISQKDLNS SNQERGISIL VAEKGTQNIQ ADGSRYLILH NGYRYDGNPG QANYRAIQYD TYGVMLPKPE ASSEVSERDA VPTADLFGSD NPRYQAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccccagg gcatcaacca gttcgccctg ctgttgaaca agcaggatac cctgaccgag ttcgacaccc tggtcccggg ccgtttccag gcaatgcgcg acggtacccg ggtgacctat accgaggaac tgtcgaagga tcgcggcgaa ctggccggca tcttcatttc gcagaaggac ctcaacagca gcaaccagga acgcggcatc tccatcctgg tggcggagaa gggcacccag aacatccagg cggatggcag ccgttacctg atcctgcaca acggctaccg ctacgacggc aatcccgggc aggccaacta ccgggcgatc cagtacgaca cctatggcgt gatgctgccc aagcccgagg ccagcagcga ggtgagcgag cgtgacgcgg tgcccaccgc cgacctgttc ggcagcgaca acccacgcta ccaggccgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat