PsaeA.18849.a
Valine--tRNA ligase (EC 6.1.1.9) (Valyl-tRNA synthetase) (ValRS)
| CENTER ID: | PsaeA.18849.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.18849.a.B1.GE38432 | full length | 1 | 950 | ||
| PsaeA.18849.a.B3.GE38434 | aatRS domain, class Ia | 14 | 628 | ||
| PsaeA.18849.a.B4.GE38435 | aatRS domain, class Ia, anticodon-binding | 639 | 898 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.18849.a.B4.PW37589 | aatRS domain, class Ia, anticodon-binding | 639 | 898 |
Structures
4XKZ
DEPOSITED: 1/12/2015
DETERMINATION: XRay
CLONE: PsaeA.18849.a.B4.GE38435
PROTEIN: PsaeA.18849.a.B4.PW37589
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.4014 |
| PGAT: | pseudomonas:PA3834 |
| RefSeq: | NP_252523.1 |
| UniProt: | Q9HXH0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKTYQPHAI ETSWYETWES NDYFAPSGEG QPYTIMIPPP NVTGSLHMGH GFNNAIMDAL IRYRRMQGRN TLWQPGTDHA GIATQMVVER QLGAQGVSRH DLGREKFLEK VWEWKEQSGG NITRQIRRLG SSVDWSRERF TMDDGLSEAV KEAFVRLHED GLIYRGKRLV NWDTKLHTAI SDLEVENHDE KGHLWHLRYP LVNGAKTSEG LDYLVVATTR PETLLGDAAV AVHPEDERYA KLIGQFAELP IVGRHIPIIA DEYVDREFGT GCVKITPAHD FNDYEVGKRH DLPLINIFDK NAAVLAQAQV FHLDGSVNPN LDPSLPQSYA GMDRFAARKA IVAEFEAMGL LEKVDDHALK VPKGDRSGTV IEPWLTDQWY VSTKPLAEDA IAAVEDGRIQ FVPKQYENMY FSWMRDIQDW CISRQLWWGH RIPAWYDEAG NVYVGRDEVE VRTKHKLGNE AELRQDEDVL DTWFSSGLWT FSTLGWPQQT EFLKTFHPTD VLVTGFDIIF FWVARMIMLT MHLVKNPDGT PQIPFKTVYV HGLVRDGQGQ KMSKSKGNVL DPLDIVDGID LDTLLQKRTS GMMQPKLAEK IAKQTRAEFP EGIASYGTDA LRFTFCSLAS TGRDIKFDMG RVEGFRNFCN KIWNAANFVI ENTDGQDTGV NGEPVELSSV DRWIISQLQR TEQEVTRQLD AFRFDLAAQA LYEFIWDEYC AWYLELVKPV LWDENAPIER QRGTRRTLIR VLETALRLAH PFMPFITEEI WQRIKGQAGK EGPTLMLQPW PVADEGRIDA AAEGDIEWVK ALMLGVRQIR GEMNISMAKR IDIILKNASP SDHRRLADNE PLLMKLAKLE SIRVLEAGEE APMSATALVG DMEVLVPMAG LIDKSAELGR LDKEIQRLEG EVKRVGGKLS NEGFVAKAPA DVIEKERAKL AEAEQALAKL AEQRQKIAAL
NT Sequence
atggacaaga cttaccagcc gcacgccatc gagacttcct ggtacgagac ctgggagtcc aacgactact tcgctccgag cggcgaaggc cagccctaca ccatcatgat cccgccgccg aacgtgaccg gcagcctgca catgggccat ggcttcaaca acgcgatcat ggacgcgctg atccgctacc gccgcatgca gggccgcaac accctgtggc agccgggcac cgatcacgcc ggcatcgcca cccagatggt ggtcgagcgc cagctcggcg cccagggcgt gagccgccac gacctcggcc gggagaagtt cctggagaag gtctgggaat ggaaggaaca gtccggcggc aacatcaccc ggcagattcg tcgcctgggc tcctcggtgg actggtcgcg cgagcgcttc accatggacg acggcctctc cgaagcggtc aaggaagcct tcgtgcgcct gcatgaagac ggcctgatct accgcggcaa acgcctggtg aactgggaca ccaagctgca caccgcgatt tccgacctgg aagtggaaaa ccacgacgag aagggccacc tctggcacct gcgctacccg ctggtgaacg gcgcgaagac cagcgaaggc ctggattacc tggtggtcgc caccacccgt ccggaaaccc tgctgggcga cgccgcggtc gcggtgcacc cggaagacga gcgctacgcc aagctgatcg gccagttcgc cgaactgccg atcgtcggcc ggcacatccc gatcatcgcc gacgagtacg tcgaccgcga attcggcacc ggctgcgtga agatcacccc ggcccacgac ttcaacgact acgaagtggg caagcgccac gacctgccgc tgatcaacat cttcgacaag aacgccgcgg tgctggccca ggcccaggtg ttccacctcg acggcagcgt caacccgaac ctcgacccca gcctgccgca gagctacgcc ggcatggacc gcttcgccgc gcgcaaggcc atcgtcgccg agttcgaggc catgggcttg ctcgagaagg tcgacgacca cgcgctgaaa gtaccgaagg gcgaccgctc cggcaccgtc atcgagccct ggctgaccga ccagtggtac gtcagcacca agccgctggc cgaagacgcc atcgccgccg tggaagacgg ccgtatccag ttcgtgccca agcagtacga gaacatgtac ttctcctgga tgcgcgacat ccaggactgg tgcatcagcc gccagctctg gtggggccac cgcatcccgg cctggtacga cgaggccggc aacgtctacg tcggccgcga cgaggtcgag gtgcgcacca agcacaagct cggcaacgag gccgagttgc gccaggacga agacgttctc gacacctggt tcagctccgg cctgtggacc ttctccaccc tcggctggcc gcagcagacc gagttcctca agaccttcca ccccaccgac gtactggtca ccggcttcga catcatcttc ttctgggtcg cgcggatgat catgctcacc atgcacctgg tgaagaaccc cgacggcacc ccgcagatcc cgttcaagac cgtctatgtc cacggcctgg tgcgcgacgg ccagggccag aagatgtcca agtccaaggg caacgtactc gacccgctgg acatcgtcga cggcatcgac ctcgataccc tgctgcaaaa gcgcaccagc ggcatgatgc agccgaagct ggcggagaag atcgccaagc agacccgcgc cgaattcccc gaaggcatcg ccagctacgg caccgacgcc ctgcgcttca ccttctgctc gctggcctcc accggccgcg acatcaagtt cgacatgggc cgcgtcgaag gcttccgcaa cttctgcaac aagatctgga acgccgccaa cttcgtcatc gagaacaccg atggccagga caccggggtc aacggcgagc cggtcgagct gtcctcggtc gaccgctgga tcatctccca gttgcagcgc accgagcagg aagtcacccg ccagctcgac gccttccgct tcgatctggc cgcccaggcc ctctatgagt tcatctggga cgagtactgc gcctggtacc tggagctggt caagccggtg ctgtgggacg agaacgcccc catcgagcgc cagcgcggca ctcgtcgcac cctgatccgc gtgctggaga cggccctgcg cctggcgcac ccgttcatgc cgttcatcac cgaggaaatc tggcaacgca tcaagggcca ggccggcaag gaaggcccga ccctgatgct gcaaccctgg ccggtggccg acgaaggcag gatcgacgcc gccgccgaag gcgacatcga atgggtcaag gcgctgatgc tcggcgtgcg gcagatccgc ggcgagatga acatctccat ggccaagcgc atcgacatca tcctcaagaa cgcctcgccc agcgaccacc gccgcctggc cgacaacgag ccgctgctga tgaaactggc caagctggaa agcatccgcg tgctggaagc cggcgaggaa gcgccgatgt ccgccaccgc cctggtcggc gacatggaag tattggtgcc gatggccggc ctgatcgaca agagcgccga actggggcgc ctggacaagg agatccagcg cctggaaggc gaggtcaagc gcgtcggcgg caagctgtcc aacgaaggct tcgtcgccaa ggccccggcc gacgtgatcg agaaggaacg cgccaagcta gccgaggccg aacaggccct ggccaagctg gccgagcaac ggcagaagat cgccgcgctc taa
Details for PsaeA.18849.a.B1.GE38432
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 109kDa |
| OBSERVED MW: | 80kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMD KTYQPHAIET SWYETWESND YFAPSGEGQP YTIMIPPPNV TGSLHMGHGF NNAIMDALIR YRRMQGRNTL WQPGTDHAGI ATQMVVERQL GAQGVSRHDL GREKFLEKVW EWKEQSGGNI TRQIRRLGSS VDWSRERFTM DDGLSEAVKE AFVRLHEDGL IYRGKRLVNW DTKLHTAISD LEVENHDEKG HLWHLRYPLV NGAKTSEGLD YLVVATTRPE TLLGDAAVAV HPEDERYAKL IGQFAELPIV GRHIPIIADE YVDREFGTGC VKITPAHDFN DYEVGKRHDL PLINIFDKNA AVLAQAQVFH LDGSVNPNLD PSLPQSYAGM DRFAARKAIV AEFEAMGLLE KVDDHALKVP KGDRSGTVIE PWLTDQWYVS TKPLAEDAIA AVEDGRIQFV PKQYENMYFS WMRDIQDWCI SRQLWWGHRI PAWYDEAGNV YVGRDEVEVR TKHKLGNEAE LRQDEDVLDT WFSSGLWTFS TLGWPQQTEF LKTFHPTDVL VTGFDIIFFW VARMIMLTMH LVKNPDGTPQ IPFKTVYVHG LVRDGQGQKM SKSKGNVLDP LDIVDGIDLD TLLQKRTSGM MQPKLAEKIA KQTRAEFPEG IASYGTDALR FTFCSLASTG RDIKFDMGRV EGFRNFCNKI WNAANFVIEN TDGQDTGVNG EPVELSSVDR WIISQLQRTE QEVTRQLDAF RFDLAAQALY EFIWDEYCAW YLELVKPVLW DENAPIERQR GTRRTLIRVL ETALRLAHPF MPFITEEIWQ RIKGQAGKEG PTLMLQPWPV ADEGRIDAAA EGDIEWVKAL MLGVRQIRGE MNISMAKRID IILKNASPSD HRRLADNEPL LMKLAKLESI RVLEAGEEAP MSATALVGDM EVLVPMAGLI DKSAELGRLD KEIQRLEGEV KRVGGKLSNE GFVAKAPADV IEKERAKLAE AEQALAKLAE QRQKIAAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca agacttacca gccgcacgcc atcgagactt cctggtacga gacctgggag tccaacgact acttcgctcc gagcggcgaa ggccagccct acaccatcat gatcccgccg ccgaacgtga ccggcagcct gcacatgggc catggcttca acaacgcgat catggacgcg ctgatccgct accgccgcat gcagggccgc aacaccctgt ggcagccggg caccgatcac gccggcatcg ccacccagat ggtggtcgag cgccagctcg gcgcccaggg cgtgagccgc cacgacctcg gccgggagaa gttcctggag aaggtctggg aatggaagga acagtccggc ggcaacatca cccggcagat tcgtcgcctg ggctcctcgg tggactggtc gcgcgagcgc ttcaccatgg acgacggcct ctccgaagcg gtcaaggaag ccttcgtgcg cctgcatgaa gacggcctga tctaccgcgg caaacgcctg gtgaactggg acaccaagct gcacaccgcg atttccgacc tggaagtgga aaaccacgac gagaagggcc acctctggca cctgcgctac ccgctggtga acggcgcgaa gaccagcgaa ggcctggatt acctggtggt cgccaccacc cgtccggaaa ccctgctggg cgacgccgcg gtcgcggtgc acccggaaga cgagcgctac gccaagctga tcggccagtt cgccgaactg ccgatcgtcg gccggcacat cccgatcatc gccgacgagt acgtcgaccg cgaattcggc accggctgcg tgaagatcac cccggcccac gacttcaacg actacgaagt gggcaagcgc cacgacctgc cgctgatcaa catcttcgac aagaacgccg cggtgctggc ccaggcccag gtgttccacc tcgacggcag cgtcaacccg aacctcgacc ccagcctgcc gcagagctac gccggcatgg accgcttcgc cgcgcgcaag gccatcgtcg ccgagttcga ggccatgggc ttgctcgaga aggtcgacga ccacgcgctg aaagtaccga agggcgaccg ctccggcacc gtcatcgagc cctggctgac cgaccagtgg tacgtcagca ccaagccgct ggccgaagac gccatcgccg ccgtggaaga cggccgtatc cagttcgtgc ccaagcagta cgagaacatg tacttctcct ggatgcgcga catccaggac tggtgcatca gccgccagct ctggtggggc caccgcatcc cggcctggta cgacgaggcc ggcaacgtct acgtcggccg cgacgaggtc gaggtgcgca ccaagcacaa gctcggcaac gaggccgagt tgcgccagga cgaagacgtt ctcgacacct ggttcagctc cggcctgtgg accttctcca ccctcggctg gccgcagcag accgagttcc tcaagacctt ccaccccacc gacgtactgg tcaccggctt cgacatcatc ttcttctggg tcgcgcggat gatcatgctc accatgcacc tggtgaagaa ccccgacggc accccgcaga tcccgttcaa gaccgtctat gtccacggcc tggtgcgcga cggccagggc cagaagatgt ccaagtccaa gggcaacgta ctcgacccgc tggacatcgt cgacggcatc gacctcgata ccctgctgca aaagcgcacc agcggcatga tgcagccgaa gctggcggag aagatcgcca agcagacccg cgccgaattc cccgaaggca tcgccagcta cggcaccgac gccctgcgct tcaccttctg ctcgctggcc tccaccggcc gcgacatcaa gttcgacatg ggccgcgtcg aaggcttccg caacttctgc aacaagatct ggaacgccgc caacttcgtc atcgagaaca ccgatggcca ggacaccggg gtcaacggcg agccggtcga gctgtcctcg gtcgaccgct ggatcatctc ccagttgcag cgcaccgagc aggaagtcac ccgccagctc gacgccttcc gcttcgatct ggccgcccag gccctctatg agttcatctg ggacgagtac tgcgcctggt acctggagct ggtcaagccg gtgctgtggg acgagaacgc ccccatcgag cgccagcgcg gcactcgtcg caccctgatc cgcgtgctgg agacggccct gcgcctggcg cacccgttca tgccgttcat caccgaggaa atctggcaac gcatcaaggg ccaggccggc aaggaaggcc cgaccctgat gctgcaaccc tggccggtgg ccgacgaagg caggatcgac gccgccgccg aaggcgacat cgaatgggtc aaggcgctga tgctcggcgt gcggcagatc cgcggcgaga tgaacatctc catggccaag cgcatcgaca tcatcctcaa gaacgcctcg cccagcgacc accgccgcct ggccgacaac gagccgctgc tgatgaaact ggccaagctg gaaagcatcc gcgtgctgga agccggcgag gaagcgccga tgtccgccac cgccctggtc ggcgacatgg aagtattggt gccgatggcc ggcctgatcg acaagagcgc cgaactgggg cgcctggaca aggagatcca gcgcctggaa ggcgaggtca agcgcgtcgg cggcaagctg tccaacgaag gcttcgtcgc caaggccccg gccgacgtga tcgagaagga acgcgccaag ctagccgagg ccgaacaggc cctggccaag ctggccgagc aacggcagaa gatcgccgcg ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.18849.a.B3.GE38434
| HARVESTED ON: | 8/29/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 71kDa |
| OBSERVED MW: | 67kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHWY ETWESNDYFA PSGEGQPYTI MIPPPNVTGS LHMGHGFNNA IMDALIRYRR MQGRNTLWQP GTDHAGIATQ MVVERQLGAQ GVSRHDLGRE KFLEKVWEWK EQSGGNITRQ IRRLGSSVDW SRERFTMDDG LSEAVKEAFV RLHEDGLIYR GKRLVNWDTK LHTAISDLEV ENHDEKGHLW HLRYPLVNGA KTSEGLDYLV VATTRPETLL GDAAVAVHPE DERYAKLIGQ FAELPIVGRH IPIIADEYVD REFGTGCVKI TPAHDFNDYE VGKRHDLPLI NIFDKNAAVL AQAQVFHLDG SVNPNLDPSL PQSYAGMDRF AARKAIVAEF EAMGLLEKVD DHALKVPKGD RSGTVIEPWL TDQWYVSTKP LAEDAIAAVE DGRIQFVPKQ YENMYFSWMR DIQDWCISRQ LWWGHRIPAW YDEAGNVYVG RDEVEVRTKH KLGNEAELRQ DEDVLDTWFS SGLWTFSTLG WPQQTEFLKT FHPTDVLVTG FDIIFFWVAR MIMLTMHLVK NPDGTPQIPF KTVYVHGLVR DGQGQKMSKS KGNVLDPLDI VDGIDLDTLL QKRTSGMMQP KLAEKIAKQT RAEFPEGIAS YGTDALRFTF CSLASTGRDI KFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattggtacg agacctggga gtccaacgac tacttcgctc cgagcggcga aggccagccc tacaccatca tgatcccgcc gccgaacgtg accggcagcc tgcacatggg ccatggcttc aacaacgcga tcatggacgc gctgatccgc taccgccgca tgcagggccg caacaccctg tggcagccgg gcaccgatca cgccggcatc gccacccaga tggtggtcga gcgccagctc ggcgcccagg gcgtgagccg ccacgacctc ggccgggaga agttcctgga gaaggtctgg gaatggaagg aacagtccgg cggcaacatc acccggcaga ttcgtcgcct gggctcctcg gtggactggt cgcgcgagcg cttcaccatg gacgacggcc tctccgaagc ggtcaaggaa gccttcgtgc gcctgcatga agacggcctg atctaccgcg gcaaacgcct ggtgaactgg gacaccaagc tgcacaccgc gatttccgac ctggaagtgg aaaaccacga cgagaagggc cacctctggc acctgcgcta cccgctggtg aacggcgcga agaccagcga aggcctggat tacctggtgg tcgccaccac ccgtccggaa accctgctgg gcgacgccgc ggtcgcggtg cacccggaag acgagcgcta cgccaagctg atcggccagt tcgccgaact gccgatcgtc ggccggcaca tcccgatcat cgccgacgag tacgtcgacc gcgaattcgg caccggctgc gtgaagatca ccccggccca cgacttcaac gactacgaag tgggcaagcg ccacgacctg ccgctgatca acatcttcga caagaacgcc gcggtgctgg cccaggccca ggtgttccac ctcgacggca gcgtcaaccc gaacctcgac cccagcctgc cgcagagcta cgccggcatg gaccgcttcg ccgcgcgcaa ggccatcgtc gccgagttcg aggccatggg cttgctcgag aaggtcgacg accacgcgct gaaagtaccg aagggcgacc gctccggcac cgtcatcgag ccctggctga ccgaccagtg gtacgtcagc accaagccgc tggccgaaga cgccatcgcc gccgtggaag acggccgtat ccagttcgtg cccaagcagt acgagaacat gtacttctcc tggatgcgcg acatccagga ctggtgcatc agccgccagc tctggtgggg ccaccgcatc ccggcctggt acgacgaggc cggcaacgtc tacgtcggcc gcgacgaggt cgaggtgcgc accaagcaca agctcggcaa cgaggccgag ttgcgccagg acgaagacgt tctcgacacc tggttcagct ccggcctgtg gaccttctcc accctcggct ggccgcagca gaccgagttc ctcaagacct tccaccccac cgacgtactg gtcaccggct tcgacatcat cttcttctgg gtcgcgcgga tgatcatgct caccatgcac ctggtgaaga accccgacgg caccccgcag atcccgttca agaccgtcta tgtccacggc ctggtgcgcg acggccaggg ccagaagatg tccaagtcca agggcaacgt actcgacccg ctggacatcg tcgacggcat cgacctcgat accctgctgc aaaagcgcac cagcggcatg atgcagccga agctggcgga gaagatcgcc aagcagaccc gcgccgaatt ccccgaaggc atcgccagct acggcaccga cgccctgcgc ttcaccttct gctcgctggc ctccaccggc cgcgacatca agttcgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for PsaeA.18849.a.B4.GE38435
| HARVESTED ON: | 8/29/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMC NKIWNAANFV IENTDGQDTG VNGEPVELSS VDRWIISQLQ RTEQEVTRQL DAFRFDLAAQ ALYEFIWDEY CAWYLELVKP VLWDENAPIE RQRGTRRTLI RVLETALRLA HPFMPFITEE IWQRIKGQAG KEGPTLMLQP WPVADEGRID AAAEGDIEWV KALMLGVRQI RGEMNISMAK RIDIILKNAS PSDHRRLADN EPLLMKLAKL ESIRVLEAGE EAPMSATALV GDMEVLVPMA GLIDKSAELG RLDKEIQRL
Validated NT Sequence
atcttactca cttacaggcg ctggatctcc ttgtccaggc gccccagttc ggcgctcttg tcgatcaggc cggccatcgg caccaatact tccatgtcgc cgaccagggc ggtggcggac atcggcgctt cctcgccggc ttccagcacg cggatgcttt ccagcttggc cagtttcatc agcagcggct cgttgtcggc caggcggcgg tggtcgctgg gcgaggcgtt cttgaggatg atgtcgatgc gcttggccat ggagatgttc atctcgccgc ggatctgccg cacgccgagc atcagcgcct tgacccattc gatgtcgcct tcggcggcgg cgtcgatcct gccttcgtcg gccaccggcc agggttgcag catcagggtc gggccttcct tgccggcctg gcccttgatg cgttgccaga tttcctcggt gatgaacggc atgaacgggt gcgccaggcg cagggccgtc tccagcacgc ggatcagggt gcgacgagtg ccgcgctggc gctcgatggg ggcgttctcg tcccacagca ccggcttgac cagctccagg taccaggcgc agtactcgtc ccagatgaac tcatagaggg cctgggcggc cagatcgaag cggaaggcgt cgagctggcg ggtgacttcc tgctcggtgc gctgcaactg ggagatgatc cagcggtcga ccgaggacag ctcgaccggc tcgccgttga ccccggtgtc ctggccatcg gtgttctcga tgacgaagtt ggcggcgttc cagatcttgt tgcacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHCN KIWNAANFVI ENTDGQDTGV NGEPVELSSV DRWIISQLQR TEQEVTRQLD AFRFDLAAQA LYEFIWDEYC AWYLELVKPV LWDENAPIER QRGTRRTLIR VLETALRLAH PFMPFITEEI WQRIKGQAGK EGPTLMLQPW PVADEGRIDA AAEGDIEWVK ALMLGVRQIR GEMNISMAKR IDIILKNASP SDHRRLADNE PLLMKLAKLE SIRVLEAGEE APMSATALVG DMEVLVPMAG LIDKSAELGR LDKEIQRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgcaaca agatctggaa cgccgccaac ttcgtcatcg agaacaccga tggccaggac accggggtca acggcgagcc ggtcgagctg tcctcggtcg accgctggat catctcccag ttgcagcgca ccgagcagga agtcacccgc cagctcgacg ccttccgctt cgatctggcc gcccaggccc tctatgagtt catctgggac gagtactgcg cctggtacct ggagctggtc aagccggtgc tgtgggacga gaacgccccc atcgagcgcc agcgcggcac tcgtcgcacc ctgatccgcg tgctggagac ggccctgcgc ctggcgcacc cgttcatgcc gttcatcacc gaggaaatct ggcaacgcat caagggccag gccggcaagg aaggcccgac cctgatgctg caaccctggc cggtggccga cgaaggcagg atcgacgccg ccgccgaagg cgacatcgaa tgggtcaagg cgctgatgct cggcgtgcgg cagatccgcg gcgagatgaa catctccatg gccaagcgca tcgacatcat cctcaagaac gcctcgccca gcgaccaccg ccgcctggcc gacaacgagc cgctgctgat gaaactggcc aagctggaaa gcatccgcgt gctggaagcc ggcgaggaag cgccgatgtc cgccaccgcc ctggtcggcg acatggaagt attggtgccg atggccggcc tgatcgacaa gagcgccgaa ctggggcgcc tggacaagga gatccagcgc ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.18849.a.B4.PW37589
| PURIFICATION DATe: | 11/5/2014 |
| CONCENTRATION: | 18mg/ml |
| OBSERVED MW: | 31kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC NKIWNAANFV IENTDGQDTG VNGEPVELSS VDRWIISQLQ RTEQEVTRQL DAFRFDLAAQ ALYEFIWDEY CAWYLELVKP VLWDENAPIE RQRGTRRTLI RVLETALRLA HPFMPFITEE IWQRIKGQAG KEGPTLMLQP WPVADEGRID AAAEGDIEWV KALMLGVRQI RGEMNISMAK RIDIILKNAS PSDHRRLADN EPLLMKLAKL ESIRVLEAGE EAPMSATALV GDMEVLVPMA GLIDKSAELG RLDKEIQRL
Validated NT Sequence
atcttactca cttacaggcg ctggatctcc ttgtccaggc gccccagttc ggcgctcttg tcgatcaggc cggccatcgg caccaatact tccatgtcgc cgaccagggc ggtggcggac atcggcgctt cctcgccggc ttccagcacg cggatgcttt ccagcttggc cagtttcatc agcagcggct cgttgtcggc caggcggcgg tggtcgctgg gcgaggcgtt cttgaggatg atgtcgatgc gcttggccat ggagatgttc atctcgccgc ggatctgccg cacgccgagc atcagcgcct tgacccattc gatgtcgcct tcggcggcgg cgtcgatcct gccttcgtcg gccaccggcc agggttgcag catcagggtc gggccttcct tgccggcctg gcccttgatg cgttgccaga tttcctcggt gatgaacggc atgaacgggt gcgccaggcg cagggccgtc tccagcacgc ggatcagggt gcgacgagtg ccgcgctggc gctcgatggg ggcgttctcg tcccacagca ccggcttgac cagctccagg taccaggcgc agtactcgtc ccagatgaac tcatagaggg cctgggcggc cagatcgaag cggaaggcgt cgagctggcg ggtgacttcc tgctcggtgc gctgcaactg ggagatgatc cagcggtcga ccgaggacag ctcgaccggc tcgccgttga ccccggtgtc ctggccatcg gtgttctcga tgacgaagtt ggcggcgttc cagatcttgt tgcacatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHCN KIWNAANFVI ENTDGQDTGV NGEPVELSSV DRWIISQLQR TEQEVTRQLD AFRFDLAAQA LYEFIWDEYC AWYLELVKPV LWDENAPIER QRGTRRTLIR VLETALRLAH PFMPFITEEI WQRIKGQAGK EGPTLMLQPW PVADEGRIDA AAEGDIEWVK ALMLGVRQIR GEMNISMAKR IDIILKNASP SDHRRLADNE PLLMKLAKLE SIRVLEAGEE APMSATALVG DMEVLVPMAG LIDKSAELGR LDKEIQRL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTGCAACA AGATCTGGAA CGCCGCCAAC TTCGTCATCG AGAACACCGA TGGCCAGGAC ACCGGGGTCA ACGGCGAGCC GGTCGAGCTG TCCTCGGTCG ACCGCTGGAT CATCTCCCAG TTGCAGCGCA CCGAGCAGGA AGTCACCCGC CAGCTCGACG CCTTCCGCTT CGATCTGGCC GCCCAGGCCC TCTATGAGTT CATCTGGGAC GAGTACTGCG CCTGGTACCT GGAGCTGGTC AAGCCGGTGC TGTGGGACGA GAACGCCCCC ATCGAGCGCC AGCGCGGCAC TCGTCGCACC CTGATCCGCG TGCTGGAGAC GGCCCTGCGC CTGGCGCACC CGTTCATGCC GTTCATCACC GAGGAAATCT GGCAACGCAT CAAGGGCCAG GCCGGCAAGG AAGGCCCGAC CCTGATGCTG CAACCCTGGC CGGTGGCCGA CGAAGGCAGG ATCGACGCCG CCGCCGAAGG CGACATCGAA TGGGTCAAGG CGCTGATGCT CGGCGTGCGG CAGATCCGCG GCGAGATGAA CATCTCCATG GCCAAGCGCA TCGACATCAT CCTCAAGAAC GCCTCGCCCA GCGACCACCG CCGCCTGGCC GACAACGAGC CGCTGCTGAT GAAACTGGCC AAGCTGGAAA GCATCCGCGT GCTGGAAGCC GGCGAGGAAG CGCCGATGTC CGCCACCGCC CTGGTCGGCG ACATGGAAGT ATTGGTGCCG ATGGCCGGCC TGATCGACAA GAGCGCCGAA CTGGGGCGCC TGGACAAGGA GATCCAGCGC CTGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT