PsaeA.18849.a
Valine--tRNA ligase (EC 6.1.1.9) (Valyl-tRNA synthetase) (ValRS)
CENTER ID:
PsaeA.18849.a
ORGANISM:
Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:
Pseudomonas infection
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
unclassified
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.18849.a.B1.GE38432
full length
info
1
950
PsaeA.18849.a.B1.GE38432
PsaeA.18849.a.B3.GE38434
aatRS domain, class Ia
info
14
628
PsaeA.18849.a.B3.GE38434
PsaeA.18849.a.B4.GE38435
aatRS domain, class Ia, anticodon-binding
info
639
898
PsaeA.18849.a.B4.GE38435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
PsaeA.18849.a.B4.PW37589
aatRS domain, class Ia, anticodon-binding
info
639
898
PW37589
Structures
4XKZ
DEPOSITED: 1/12/2015
DETERMINATION: XRay
CLONE: PsaeA.18849.a.B4.GE38435
PROTEIN: PsaeA.18849.a.B4.PW37589
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKTYQPHAI ETSWYETWES NDYFAPSGEG QPYTIMIPPP NVTGSLHMGH GFNNAIMDAL IRYRRMQGRN TLWQPGTDHA GIATQMVVER QLGAQGVSRH DLGREKFLEK VWEWKEQSGG NITRQIRRLG SSVDWSRERF TMDDGLSEAV KEAFVRLHED GLIYRGKRLV NWDTKLHTAI SDLEVENHDE KGHLWHLRYP LVNGAKTSEG LDYLVVATTR PETLLGDAAV AVHPEDERYA KLIGQFAELP IVGRHIPIIA DEYVDREFGT GCVKITPAHD FNDYEVGKRH DLPLINIFDK NAAVLAQAQV FHLDGSVNPN LDPSLPQSYA GMDRFAARKA IVAEFEAMGL LEKVDDHALK VPKGDRSGTV IEPWLTDQWY VSTKPLAEDA IAAVEDGRIQ FVPKQYENMY FSWMRDIQDW CISRQLWWGH RIPAWYDEAG NVYVGRDEVE VRTKHKLGNE AELRQDEDVL DTWFSSGLWT FSTLGWPQQT EFLKTFHPTD VLVTGFDIIF FWVARMIMLT MHLVKNPDGT PQIPFKTVYV HGLVRDGQGQ KMSKSKGNVL DPLDIVDGID LDTLLQKRTS GMMQPKLAEK IAKQTRAEFP EGIASYGTDA LRFTFCSLAS TGRDIKFDMG RVEGFRNFCN KIWNAANFVI ENTDGQDTGV NGEPVELSSV DRWIISQLQR TEQEVTRQLD AFRFDLAAQA LYEFIWDEYC AWYLELVKPV LWDENAPIER QRGTRRTLIR VLETALRLAH PFMPFITEEI WQRIKGQAGK EGPTLMLQPW PVADEGRIDA AAEGDIEWVK ALMLGVRQIR GEMNISMAKR IDIILKNASP SDHRRLADNE PLLMKLAKLE SIRVLEAGEE APMSATALVG DMEVLVPMAG LIDKSAELGR LDKEIQRLEG EVKRVGGKLS NEGFVAKAPA DVIEKERAKL AEAEQALAKL AEQRQKIAAL
NT Sequence
atggacaaga cttaccagcc gcacgccatc gagacttcct ggtacgagac ctgggagtcc aacgactact tcgctccgag cggcgaaggc cagccctaca ccatcatgat cccgccgccg aacgtgaccg gcagcctgca catgggccat ggcttcaaca acgcgatcat ggacgcgctg atccgctacc gccgcatgca gggccgcaac accctgtggc agccgggcac cgatcacgcc ggcatcgcca cccagatggt ggtcgagcgc cagctcggcg cccagggcgt gagccgccac gacctcggcc gggagaagtt cctggagaag gtctgggaat ggaaggaaca gtccggcggc aacatcaccc ggcagattcg tcgcctgggc tcctcggtgg actggtcgcg cgagcgcttc accatggacg acggcctctc cgaagcggtc aaggaagcct tcgtgcgcct gcatgaagac ggcctgatct accgcggcaa acgcctggtg aactgggaca ccaagctgca caccgcgatt tccgacctgg aagtggaaaa ccacgacgag aagggccacc tctggcacct gcgctacccg ctggtgaacg gcgcgaagac cagcgaaggc ctggattacc tggtggtcgc caccacccgt ccggaaaccc tgctgggcga cgccgcggtc gcggtgcacc cggaagacga gcgctacgcc aagctgatcg gccagttcgc cgaactgccg atcgtcggcc ggcacatccc gatcatcgcc gacgagtacg tcgaccgcga attcggcacc ggctgcgtga agatcacccc ggcccacgac ttcaacgact acgaagtggg caagcgccac gacctgccgc tgatcaacat cttcgacaag aacgccgcgg tgctggccca ggcccaggtg ttccacctcg acggcagcgt caacccgaac ctcgacccca gcctgccgca gagctacgcc ggcatggacc gcttcgccgc gcgcaaggcc atcgtcgccg agttcgaggc catgggcttg ctcgagaagg tcgacgacca cgcgctgaaa gtaccgaagg gcgaccgctc cggcaccgtc atcgagccct ggctgaccga ccagtggtac gtcagcacca agccgctggc cgaagacgcc atcgccgccg tggaagacgg ccgtatccag ttcgtgccca agcagtacga gaacatgtac ttctcctgga tgcgcgacat ccaggactgg tgcatcagcc gccagctctg gtggggccac cgcatcccgg cctggtacga cgaggccggc aacgtctacg tcggccgcga cgaggtcgag gtgcgcacca agcacaagct cggcaacgag gccgagttgc gccaggacga agacgttctc gacacctggt tcagctccgg cctgtggacc ttctccaccc tcggctggcc gcagcagacc gagttcctca agaccttcca ccccaccgac gtactggtca ccggcttcga catcatcttc ttctgggtcg cgcggatgat catgctcacc atgcacctgg tgaagaaccc cgacggcacc ccgcagatcc cgttcaagac cgtctatgtc cacggcctgg tgcgcgacgg ccagggccag aagatgtcca agtccaaggg caacgtactc gacccgctgg acatcgtcga cggcatcgac ctcgataccc tgctgcaaaa gcgcaccagc ggcatgatgc agccgaagct ggcggagaag atcgccaagc agacccgcgc cgaattcccc gaaggcatcg ccagctacgg caccgacgcc ctgcgcttca ccttctgctc gctggcctcc accggccgcg acatcaagtt cgacatgggc cgcgtcgaag gcttccgcaa cttctgcaac aagatctgga acgccgccaa cttcgtcatc gagaacaccg atggccagga caccggggtc aacggcgagc cggtcgagct gtcctcggtc gaccgctgga tcatctccca gttgcagcgc accgagcagg aagtcacccg ccagctcgac gccttccgct tcgatctggc cgcccaggcc ctctatgagt tcatctggga cgagtactgc gcctggtacc tggagctggt caagccggtg ctgtgggacg agaacgcccc catcgagcgc cagcgcggca ctcgtcgcac cctgatccgc gtgctggaga cggccctgcg cctggcgcac ccgttcatgc cgttcatcac cgaggaaatc tggcaacgca tcaagggcca ggccggcaag gaaggcccga ccctgatgct gcaaccctgg ccggtggccg acgaaggcag gatcgacgcc gccgccgaag gcgacatcga atgggtcaag gcgctgatgc tcggcgtgcg gcagatccgc ggcgagatga acatctccat ggccaagcgc atcgacatca tcctcaagaa cgcctcgccc agcgaccacc gccgcctggc cgacaacgag ccgctgctga tgaaactggc caagctggaa agcatccgcg tgctggaagc cggcgaggaa gcgccgatgt ccgccaccgc cctggtcggc gacatggaag tattggtgcc gatggccggc ctgatcgaca agagcgccga actggggcgc ctggacaagg agatccagcg cctggaaggc gaggtcaagc gcgtcggcgg caagctgtcc aacgaaggct tcgtcgccaa ggccccggcc gacgtgatcg agaaggaacg cgccaagcta gccgaggccg aacaggccct ggccaagctg gccgagcaac ggcagaagat cgccgcgctc taa
Details for PsaeA.18849.a.B1.GE38432
HARVESTED ON:
data unavailable
SEQUENCED ON:
12/10/2014
EXPECTED MW:
109kDa
OBSERVED MW:
80kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.18849.a.B3.GE38434
HARVESTED ON:
8/29/2014
SEQUENCED ON:
12/10/2014
EXPECTED MW:
71kDa
OBSERVED MW:
67kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Insoluble
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for PsaeA.18849.a.B4.GE38435
HARVESTED ON:
8/29/2014
SEQUENCED ON:
12/10/2014
EXPECTED MW:
31kDa
OBSERVED MW:
31kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for PsaeA.18849.a.B4.PW37589
PURIFICATION DATe:
11/5/2014
CONCENTRATION:
18mg/ml
OBSERVED MW:
31kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
5
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100