PsaeA.18843.a

Uncharacterized protein (HHPRED: no hit)

CENTER ID: PsaeA.18843.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18843.a.B3.GE39843 coiled-coil truncated 12 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4857
PGAT: pseudomonas:PA4636
RefSeq: NP_253326.1
UniProt: Q9HVF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMGEFEAIRP YSDAEVPAVL QRLLSDDAFL GALVRYRFPR LAGPLGWLLK PLIAHRLARE VTGIRSVATL QDKFEPYVDH TIEKATDGVT YSGVNRLKPG CAYLFIANHR DIVMDPAFVN YAVYHAGLPT PRIAIGDNLL QKSFVSDLMR LNKSFIVHRS LSGRREKLAA YQTLSAYINH SIREDRQSVW IAQAEGRAKD GDDRTDSAIL KMFHMSRKDE PFAEMVRALH FIPVSISYEY DPCDLAKARE LHTRASTGEY RKAPGEDDAS IALGITGYKG RVHINFGAEL EGEFSDAKQL AAELDRQILG NYRLFPVHYL AYEMSENRDP GLAVPTAESL FGSEELEKAR KEWNRRLEQC PAEQRSWLVL QYANPVLNQY RIKQQS
NT Sequence
atgatgggcg agttcgaagc catccgaccc tacagtgacg ccgaagtccc cgcggtattg caacgcctcc tgagcgacga cgccttcctc ggcgccctgg tccgctaccg cttcccgcgc ctggccggtc ccctcggttg gctgctcaaa cctcttatag cccatcgcct ggcccgcgag gtcaccggca tccgcagcgt cgccacgctg caggacaagt tcgagcccta cgtcgaccac accatcgaga aggccaccga tggcgtcacc tactccgggg tcaatcgcct caagccaggc tgcgcctacc tgttcatcgc caaccaccgc gatatcgtga tggacccggc cttcgtcaac tacgccgtgt accacgccgg cctgccgacc ccgcgcatcg ccatcggcga caacctgctg cagaaatcct tcgtcagcga cctgatgcgc ctgaacaaga gcttcatcgt gcaccgttcg ctgagcggcc ggcgcgagaa gctcgccgcc taccagacgc tctccgccta catcaaccac tcgatccgcg aggaccgcca gtcggtgtgg atcgcccagg ccgaaggacg ggccaaggac ggcgacgacc gtaccgactc ggcgatactc aagatgttcc acatgagccg caaggacgaa cccttcgccg aaatggtgcg cgccctgcat ttcatcccgg tgtcgatcag ctacgaatac gacccctgcg acctggccaa ggcccgcgag ttgcacaccc gcgccagcac cggcgagtac cgcaaggcgc cgggcgagga cgatgccagc atcgccctgg gcatcaccgg ctacaagggc cgcgtgcaca tcaacttcgg tgccgagctg gagggcgagt tcagcgacgc caagcagctg gccgccgaac tggaccggca gatcctcggc aactaccgtc tgttcccggt gcactacctg gcctacgaga tgagcgagaa ccgcgacccg ggcctcgccg tccccacggc cgagagcctg ttcggcagcg aggagctgga gaaggcgcgc aaggaatgga accgccgcct ggagcaatgc ccggccgagc agcgctcctg gctggtgctg caatacgcca acccggtgct caaccaatac cggatcaagc agcaatcctg a
Details for PsaeA.18843.a.B3.GE39843
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS DAEVPAVLQR LLSDDAFLGA LVRYRFPRLA GPLGWLLKPL IAHRLAREVT GIRSVATLQD KFEPYVDHTI EKATDGVTYS GVNRLKPGCA YLFIANHRDI VMDPAFVNYA VYHAGLPTPR IAIGDNLLQK SFVSDLMRLN KSFIVHRSLS GRREKLAAYQ TLSAYINHSI REDRQSVWIA QAEGRAKDGD DRTDSAILKM FHMSRKDEPF AEMVRALHFI PVSISYEYDP CDLAKARELH TRASTGEYRK APGEDDASIA LGITGYKGRX XINFGAE
Validated NT Sequence
atcttactca cttactcggc accgaagttg atgtnnnnnc ggcccttgta gccggtgatg cccagggcga tgctggcatc gtcctcgccc ggcgccttgc ggtactcgcc ggtgctggcg cgggtgtgca actcgcgggc cttggccagg tcgcaggggt cgtattcgta gctgatcgac accgggatga aatgcagggc gcgcaccatt tcggcgaagg gttcgtcctt gcggctcatg tggaacatct tgagtatcgc cgagtcggta cggtcgtcgc cgtccttggc ccgtccttcg gcctgggcga tccacaccga ctggcggtcc tcgcggatcg agtggttgat gtaggcggag agcgtctggt aggcggcgag cttctcgcgc cggccgctca gcgaacggtg cacgatgaag ctcttgttca ggcgcatcag gtcgctgacg aaggatttct gcagcaggtt gtcgccgatg gcgatgcgcg gggtcggcag gccggcgtgg tacacggcgt agttgacgaa ggccgggtcc atcacgatat cgcggtggtt ggcgatgaac aggtaggcgc agcctggctt gaggcgattg accccggagt aggtgacgcc atcggtggcc ttctcgatgg tgtggtcgac gtagggctcg aacttgtcct gcagcgtggc gacgctgcgg atgccggtga cctcgcgggc caggcgatgg gctataagag gtttgagcag ccaaccgagg ggaccggcca ggcgcgggaa gcggtagcgg accagggcgc cgaggaaggc gtcgtcgctc aggaggcgtt gcaataccgc ggggacttcg gcgtcactca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHSD AEVPAVLQRL LSDDAFLGAL VRYRFPRLAG PLGWLLKPLI AHRLAREVTG IRSVATLQDK FEPYVDHTIE KATDGVTYSG VNRLKPGCAY LFIANHRDIV MDPAFVNYAV YHAGLPTPRI AIGDNLLQKS FVSDLMRLNK SFIVHRSLSG RREKLAAYQT LSAYINHSIR EDRQSVWIAQ AEGRAKDGDD RTDSAILKMF HMSRKDEPFA EMVRALHFIP VSISYEYDPC DLAKARELHT RASTGEYRKA PGEDDASIAL GITGYKGRVH INFGAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagtgacg ccgaagtccc cgcggtattg caacgcctcc tgagcgacga cgccttcctc ggcgccctgg tccgctaccg cttcccgcgc ctggccggtc ccctcggttg gctgctcaaa cctcttatag cccatcgcct ggcccgcgag gtcaccggca tccgcagcgt cgccacgctg caggacaagt tcgagcccta cgtcgaccac accatcgaga aggccaccga tggcgtcacc tactccgggg tcaatcgcct caagccaggc tgcgcctacc tgttcatcgc caaccaccgc gatatcgtga tggacccggc cttcgtcaac tacgccgtgt accacgccgg cctgccgacc ccgcgcatcg ccatcggcga caacctgctg cagaaatcct tcgtcagcga cctgatgcgc ctgaacaaga gcttcatcgt gcaccgttcg ctgagcggcc ggcgcgagaa gctcgccgcc taccagacgc tctccgccta catcaaccac tcgatccgcg aggaccgcca gtcggtgtgg atcgcccagg ccgaaggacg ggccaaggac ggcgacgacc gtaccgactc ggcgatactc aagatgttcc acatgagccg caaggacgaa cccttcgccg aaatggtgcg cgccctgcat ttcatcccgg tgtcgatcag ctacgaatac gacccctgcg acctggccaa ggcccgcgag ttgcacaccc gcgccagcac cggcgagtac cgcaaggcgc cgggcgagga cgatgccagc atcgccctgg gcatcaccgg ctacaagggc cgcgtgcaca tcaacttcgg tgccgagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at