PsaeA.18665.a

Probable protein kinase UbiB (EC 2.7.-.-) (Ubiquinone biosynthesis protein UbiB)

CENTER ID: PsaeA.18665.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18665.a.B2.GE38537 soluble domain 48 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5309
PGAT: pseudomonas:PA5065
RefSeq: NP_253752.1
UniProt: Q9HUB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLLAVRRLL RIQRVVIRYR LDDLILELPM LPWWLRLLGA TLPWRWLPRR KLELTRGARL RLALQDLGPI FIKFGQILST RRDLLPDDIA NELAWLQDKV PPFPPELAVK RIEEQLGAKI EQVFARFERE PLASASVAQV HAARLKSGEE VVVKVIRPNL EPVIRSDIAW LFILARLAER VSSEARRLHP VEVVSDYEKT IVDELDLLRE AANASQLRRN FEGSPLLYVP QVYWDWCRPK VLVMERIYGI PVTDLETLRD QRTDFKALAE RGVEIFFTQV FRDSFFHADM HPGNIFVSTR APWSPQYIAV DCGIVGSLTD EDQDYLARNL IAFFKRDYRK VAQLHIDSGW VPAETKVNDF EAAIRTVCEP IFEKPLKDIS FGQVLLRLFQ TARRFNMEIQ PQLVLLQKTL LNIEGLGRQL YPELDLWATA QPFLERWMRE RVSPKQLLRN FQQQVEQVPH LSQMARDTLE RLSQPHAHNA PPPEWKGSRH DWLGRLVGAV LLVGAAEVGL GQQLEAWPAW VMLAGGVFLI LRR
NT Sequence
atgaagctcc tcgctgtccg ccgcctcctg cgcatccagc gcgtcgtcat ccgctaccgc ctggacgacc tgatcctcga actgccgatg ctgccctggt ggctgcgcct gctcggtgcc accctgccct ggcgctggct gccgcggcgc aagctggaac tgacccgcgg cgcgcgcctg cgcctggcac tgcaggacct cggaccgatc ttcatcaagt tcggccagat tctctccacc cgccgcgacc tgctgcccga cgacatcgcc aacgaactgg cctggctgca ggacaaggtg ccgcccttcc cgccggagct ggcggtcaag cgcatcgagg aacagctggg cgcgaagatc gaacaggtct tcgcccgctt cgaacgcgaa ccgctggcct ccgcctcggt ggcccaggtc cacgccgcgc gcctgaagag cggcgaggaa gtggtggtga aggtgatccg gccgaacctg gaaccggtga tccgctccga catcgcctgg ctgttcatcc tcgcccgcct cgccgagcgg gtctccagcg aggcgcggcg cctgcacccg gtggaagtgg tcagcgacta cgagaagacc atcgtcgacg aactcgacct gctccgcgag gcggccaacg cctcgcagct gcgacgcaac ttcgaaggct cgccgctgct ctacgtgcca caggtctact gggactggtg ccggcccaag gtgctggtga tggagcgcat ctacggcatc ccggtgaccg acctggaaac cctgcgcgac cagcgcaccg acttcaaggc actggccgag cgcggcgtgg agatcttctt cacccaggtg ttccgcgaca gctttttcca tgccgacatg caccccggca acatcttcgt cagcacccgc gcgccctgga gcccgcaata catcgcggtg gactgcggca tcgtcggcag cctgaccgac gaggaccagg actacctggc gcgcaacctg atcgccttct tcaagcgcga ctaccgcaag gtcgcccaac tgcatatcga ctccggctgg gtaccggcgg aaaccaaggt caacgacttc gaggcggcga tccgcaccgt ctgcgaaccg atcttcgaga agccgctgaa ggacatctcc ttcggccagg tcctgctgcg cctgttccag accgcgcgcc gtttcaacat ggagatccag ccgcaactgg tgttgctgca gaagaccctg ctgaacatcg aaggcctcgg ccgccagctc tacccggaac tcgacctgtg ggccaccgcc cagccgttcc tggagcgctg gatgcgcgaa cgggtcagcc cgaagcagtt gctgcgcaat ttccagcaac aggtcgagca ggtgccgcac ctgtcgcaaa tggcccgcga caccctcgaa cgcctgtcac aaccgcacgc ccacaatgcg ccgccgccgg agtggaaagg ttcccgccac gactggctcg ggcgcctggt gggcgcggtg ctgctggtcg gcgccgccga ggtcggcctg ggccagcagc tggaagcctg gccggcgtgg gtgatgctgg ccggcggggt attcctgatc ctgcgccgct ag
Details for PsaeA.18665.a.B2.GE38537
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP RRKLELTRGA RLRLALQDLG PIFIKFGQIL STRRDLLPDD IANELAWLQD KVPPFPPELA VKRIEEQLGA KIEQVFARFE REPLASASVA QVHAARLKSG EEVVVKVIRP NLEPVIRSDI AWLFILARLA ERVSSEARRL HPVEVVSDYE KTIVDELDLL REAANASQLR RNFEGSPLLY VPQVYWDWCR PKVLVMERIY GIPVTDLETL RDQRTXFKAL AERGVEIFFT QVFRDSFFHA DMHPGNIFVS TRAPWSPQYI AVDCGIVGSL TDEDQDYLAR NLIAFFKRDY RKVAQLHIDS GWVPAETKVN DFEAAIRTVC EPIFEKPLKD ISFGQVLLRL FQTARRFNME IQPQLVLLQK TLLNIEGLGR QLYPELDLWA TAQPFLERWM RERVSPKQLL RNFQQQVEQV PHLSQMARDT LERLSQPHAH NAPPP
Validated NT Sequence
tccggcggcg gcgcattgtg ggcgtgcggt tgtgacaggc gttcgagggt gtcgcgggcc atttgcgaca ggtgcggcac ctgctcgacc tgttgctgga aattgcgcag caactgcttc gggctgaccc gttcgcgcat ccagcgctcc aggaacggct gggcggtggc ccacaggtcg agttccgggt agagctggcg gccgaggcct tcgatgttca gcagggtctt ctgcagcaac accagttgcg gctggatctc catgttgaaa cggcgcgcgg tctggaacag gcgcagcagg acctggccga aggagatgtc cttcagcggc ttctcgaaga tcggttcgca gacggtgcgg atcgccgcct cgaagtcgtt gaccttggtt tccgccggta cccagccgga gtcgatatgc agttgggcga ccttgcggta gtcgcgcttg aagaaggcga tcaggttgcg cgccaggtag tcctggtcct cgtcggtcag gctgccgacg atgccgcagt ccaccgcgat gtattgcggg ctccagggcg cgcgggtgct gacgaagatg ttgccggggt gcatgtcggc atggaaaaag ctgtcgcgga acacctgggt gaagaagatc tccacgccgc gctcggccag tgccttgaag ncggtgcgct ggtcgcgcag ggtttccagg tcggtcaccg ggatgccgta gatgcgctcc atcaccagca ccttgggccg gcaccagtcc cagtagacct gtggcacgta gagcagcggc gagccttcga agttgcgtcg cagctgcgag gcgttggccg cctcgcggag caggtcgagt tcgtcgacga tggtcttctc gtagtcgctg accacttcca ccgggtgcag gcgccgcgcc tcgctggaga cccgctcggc gaggcgggcg aggatgaaca gccaggcgat gtcggagcgg atcaccggtt ccaggttcgg ccggatcacc ttcaccacca cttcctcgcc gctcttcagg cgcgcggcgt ggacctgggc caccgaggcg gaggccagcg gttcgcgttc gaagcgggcg aagacctgtt cgatcttcgc gcccagctgt tcctcgatgc gcttgaccgc cagctccggc gggaagggcg gcaccttgtc ctgcagccag gccagttcgt tggcgatgtc gtcgggcagc aggtcgcggc gggtggagag aatctggccg aacttgatga agatcggtcc gaggtcctgc agtgccaggc gcaggcgcgc gccgcgggtc agttccagct tgcgccgcgg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHPR RKLELTRGAR LRLALQDLGP IFIKFGQILS TRRDLLPDDI ANELAWLQDK VPPFPPELAV KRIEEQLGAK IEQVFARFER EPLASASVAQ VHAARLKSGE EVVVKVIRPN LEPVIRSDIA WLFILARLAE RVSSEARRLH PVEVVSDYEK TIVDELDLLR EAANASQLRR NFEGSPLLYV PQVYWDWCRP KVLVMERIYG IPVTDLETLR DQRTDFKALA ERGVEIFFTQ VFRDSFFHAD MHPGNIFVST RAPWSPQYIA VDCGIVGSLT DEDQDYLARN LIAFFKRDYR KVAQLHIDSG WVPAETKVND FEAAIRTVCE PIFEKPLKDI SFGQVLLRLF QTARRFNMEI QPQLVLLQKT LLNIEGLGRQ LYPELDLWAT AQPFLERWMR ERVSPKQLLR NFQQQVEQVP HLSQMARDTL ERLSQPHAHN APPPEWKGSR HD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgcggc gcaagctgga actgacccgc ggcgcgcgcc tgcgcctggc actgcaggac ctcggaccga tcttcatcaa gttcggccag attctctcca cccgccgcga cctgctgccc gacgacatcg ccaacgaact ggcctggctg caggacaagg tgccgccctt cccgccggag ctggcggtca agcgcatcga ggaacagctg ggcgcgaaga tcgaacaggt cttcgcccgc ttcgaacgcg aaccgctggc ctccgcctcg gtggcccagg tccacgccgc gcgcctgaag agcggcgagg aagtggtggt gaaggtgatc cggccgaacc tggaaccggt gatccgctcc gacatcgcct ggctgttcat cctcgcccgc ctcgccgagc gggtctccag cgaggcgcgg cgcctgcacc cggtggaagt ggtcagcgac tacgagaaga ccatcgtcga cgaactcgac ctgctccgcg aggcggccaa cgcctcgcag ctgcgacgca acttcgaagg ctcgccgctg ctctacgtgc cacaggtcta ctgggactgg tgccggccca aggtgctggt gatggagcgc atctacggca tcccggtgac cgacctggaa accctgcgcg accagcgcac cgacttcaag gcactggccg agcgcggcgt ggagatcttc ttcacccagg tgttccgcga cagctttttc catgccgaca tgcaccccgg caacatcttc gtcagcaccc gcgcgccctg gagcccgcaa tacatcgcgg tggactgcgg catcgtcggc agcctgaccg acgaggacca ggactacctg gcgcgcaacc tgatcgcctt cttcaagcgc gactaccgca aggtcgccca actgcatatc gactccggct gggtaccggc ggaaaccaag gtcaacgact tcgaggcggc gatccgcacc gtctgcgaac cgatcttcga gaagccgctg aaggacatct ccttcggcca ggtcctgctg cgcctgttcc agaccgcgcg ccgtttcaac atggagatcc agccgcaact ggtgttgctg cagaagaccc tgctgaacat cgaaggcctc ggccgccagc tctacccgga actcgacctg tgggccaccg cccagccgtt cctggagcgc tggatgcgcg aacgggtcag cccgaagcag ttgctgcgca atttccagca acaggtcgag caggtgccgc acctgtcgca aatggcccgc gacaccctcg aacgcctgtc acaaccgcac gcccacaatg cgccgccgcc ggagtggaaa ggttcccgcc acgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat