PsaeA.18615.a

Outer membrane protein assembly factor BamA

CENTER ID: PsaeA.18615.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18615.a.B2.GE38536 m 21 797
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3817
PGAT: pseudomonas:PA3648
RefSeq: NP_252338.1
UniProt: Q9HXY4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRFLLPALL SALMIAEVHA ESFTVSDIRV NGLQRVSAGS VFAALPLNVG ETIDDQALVQ ATRSLFKTGF FQDIQLGRDG NVLVVTVVER PSISSIEIEG NKAISKEDLL KGLKQSGLAE GEIFQRATLE GVRNELQRQY VAQGRYSAEI NAEVIPQPRN RVALKININE GTVAAISHIN VVGNTVFSEE DLTDLFELKT TNWLSFFKND DKYAREKLSG DLERLRSYYL DRGYINMDIA STQVSITPDK KHVYITVNIN EGEKYTIRDV KLTGDLKVPE EEVKRLLLVQ KGQVFSRKVM TTTSDLITRR LGNEGYTFAN VNGVPEAHDD DKTVSVTFVV DPGKRAYVNR INFRGNTKTE DEVLRREMRQ MEGGWASTYL IDQSKARLER LGYFKEVNVE TPAVPGTDDQ VDVNYSVEEQ PSGSITASVG FAQSAGLILG GSISQNNFLG TGNKVSIGLT RSEYQTRYNF GFVDPYWTVD GVSLGYNAFY RKTDYDELDV DVASYSVNSL GAGMSIGYPI SETSRLTYGL SVQRDQIDTG RYTVDEIYDF LDKEGDNFTN FKASIGWSES TLNKGVLATR GHSQSLTLET TLPGSDLSFY KIDYRGQVFA PLTDNYTMRF HTELGYGDGY GSTERLPFYE NYYAGGFNSV RGFKDSTLGP RSTPSVARNP DGTPMKNQGP DSKGRYTDPD QDPEAFGGNI LITGGAELLF PLPFVKDQRQ LRTVLFWDVG STFDTDCPTK TTTNCDGIKT DNLASSVGVG LTWITALGPL SFSLATPIKK PDNAETQVFQ FSLGQTF
NT Sequence
atgaaacgct ttctgctacc cgcgctgctg tcagcgctga tgatcgccga ggttcacgcc gagtccttca ctgtttccga tatccgggtc aatggcctgc agcgcgtgtc cgccggtagc gtgttcgccg ccctgccgct gaacgtcggc gagaccatcg acgaccaggc cctggtccag gccacccgct ccctgttcaa gaccggcttc ttccaggaca tccagctcgg ccgtgatggc aacgtgctgg tagtcactgt agtcgagcgc ccgtcgatct ccagcatcga gatcgagggc aacaaggcga tctccaagga agacctgctc aagggcctga aacagtccgg cctggccgag ggcgagatct tccagcgcgc caccctcgaa ggcgtgcgca acgagctgca acgccagtac gtggcccagg gccgctactc ggccgagatc aacgcggaag tgatcccgca gccgcgcaac cgcgtcgcgc tgaagatcaa catcaacgaa ggcacggtcg ccgcgatttc ccacatcaac gtggtgggca acaccgtctt ctccgaagaa gacctgaccg acctgttcga actgaagacc accaactggc tgtcgttctt caagaacgac gacaagtacg cccgcgaaaa gctctccggc gacctcgagc gcctgcgctc ctattacctg gaccgcggct acatcaacat ggatatcgcc tccacccagg tatccatcac cccggacaag aagcacgtct acatcaccgt caacatcaac gagggtgaga agtacaccat ccgcgacgtg aagctgaccg gcgacctgaa ggtgccggaa gaggaagtga agcgcctgct gctggtacag aagggccagg tgttctcgcg caaggtgatg accaccacct ccgacctaat cacccgtcgc ctgggcaacg agggctacac cttcgccaac gtcaacggcg tgccggaagc ccatgacgac gacaagaccg tctcggtgac cttcgtcgtc gacccgggca agcgcgccta cgtcaaccgc atcaacttcc gcggcaacac caagaccgaa gacgaagtgc tgcgccgcga aatgcgccag atggaaggcg gctgggcctc cacctacctg atcgaccagt ccaaggcccg tctggagcgc ctcggctact tcaaggaagt caacgtcgag accccggcgg ttcccggcac cgacgaccag gtcgacgtca actacagcgt cgaagagcaa ccgtccggct cgatcaccgc cagcgtcggc ttcgcccaga gcgccggcct gatcctcggc ggctcgatca gccagaacaa cttcctgggt accggcaaca aggtcagcat cggcctgacc cgcagcgaat accaaacccg ctacaacttc ggcttcgtcg acccctactg gaccgtcgac ggcgtcagcc tcggctacaa tgccttctac cggaagaccg actacgacga actcgacgtc gacgtcgcca gctactcggt gaacagcctg ggcgccggca tgagcatcgg ctacccgatc agcgagacct cgcgcctgac ctatggcctg agcgtgcagc gcgaccagat cgacaccggc cgctacaccg tggacgagat ctacgacttc ctcgacaagg aaggcgacaa cttcaccaac ttcaaggctt ccatcggctg gtccgaatcg accctgaaca agggcgtact ggccacccgt ggtcactcgc agagcctgac cctggaaacc accctgccgg gcagcgacct gtcgttctac aagatcgact accgtggcca ggtcttcgcc ccgctgaccg acaactacac catgcgcttc cacaccgagc tgggctatgg cgacggctac ggatccaccg agcgcctgcc gttctacgag aactactacg ccggcggctt caactcggtg cggggcttca aggacagcac gctgggcccg cgcagtacgc cctctgtggc tcgtaatcca gacggtacgc ctatgaagaa ccaagggcct gacagcaagg gccgttacac cgacccggac caggatcccg aagccttcgg tggtaacatc ctgatcaccg gcggcgccga gctgctgttc ccgctgccgt tcgtcaagga ccagcgccag ttgcgtaccg tgctgttctg ggacgtgggt agcaccttcg ataccgactg cccgaccaag accaccacca actgcgacgg catcaagacc gacaacctcg ccagttccgt gggcgtcggc ctgacctgga tcaccgcgct gggcccgctg agcttcagcc tggcgacgcc gatcaagaag ccggacaacg cggaaaccca ggtcttccag ttctccctgg gccagacctt ctga
Details for PsaeA.18615.a.B2.GE38536
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHES FTVSDIRVNG LQRVSAGSVF AALPLNVGET IDDQALVQAT RSLFKTGFFQ DIQLGRDGNV LVVTVVERPS ISSIEIEGNK AISKEDLLKG LKQSGLAEGE IFQRATLEGV RNELQRQYVA QGRYSAEINA EVIPQPRNRV ALKININEGT VAAISHINVV GNTVFSEEDL TDLFELKTTN WLSFFKNDDK YAREKLSGDL ERLRSYYLDR GYINMDIAST QVSITPDKKH VYITVNINEG EKYTIRDVKL TGDLKVPEEE VKRLLLVQKG QVFSRKVMTT TSDLITRRLG NEGYTFANVN GVPEAHDDDK TVSVTFVVDP GKRAYVNRIN FRGNTKTEDE VLRREMRQME GGWASTYLID QSKARLERLG YFKEVNVETP AVPGTDDQVD VNYSVEEQPS GSITASVGFA QSAGLILGGS ISQNNFLGTG NKVSIGLTRS EYQTRYNFGF VDPYWTVDGV SLGYNAFYRK TDYDELDVDV ASYSVNSLGA GMSIGYPISE TSRLTYGLSV QRDQIDTGRY TVDEIYDFLD KEGDNFTNFK ASIGWSESTL NKGVLATRGH SQSLTLETTL PGSDLSFYKI DYRGQVFAPL TDNYTMRFHT ELGYGDGYGS TERLPFYENY YAGGFNSVRG FKDSTLGPRS TPSVARNPDG TPMKNQGPDS KGRYTDPDQD PEAFGGNILI TGGAELLFPL PFVKDQRQLR TVLFWDVGST FDTDCPTKTT TNCDGIKTDN LASSVGVGLT WITALGPLSF SLATPIKKPD NAETQVFQFS LGQTF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagtcct tcactgtttc cgatatccgg gtcaatggcc tgcagcgcgt gtccgccggt agcgtgttcg ccgccctgcc gctgaacgtc ggcgagacca tcgacgacca ggccctggtc caggccaccc gctccctgtt caagaccggc ttcttccagg acatccagct cggccgtgat ggcaacgtgc tggtagtcac tgtagtcgag cgcccgtcga tctccagcat cgagatcgag ggcaacaagg cgatctccaa ggaagacctg ctcaagggcc tgaaacagtc cggcctggcc gagggcgaga tcttccagcg cgccaccctc gaaggcgtgc gcaacgagct gcaacgccag tacgtggccc agggccgcta ctcggccgag atcaacgcgg aagtgatccc gcagccgcgc aaccgcgtcg cgctgaagat caacatcaac gaaggcacgg tcgccgcgat ttcccacatc aacgtggtgg gcaacaccgt cttctccgaa gaagacctga ccgacctgtt cgaactgaag accaccaact ggctgtcgtt cttcaagaac gacgacaagt acgcccgcga aaagctctcc ggcgacctcg agcgcctgcg ctcctattac ctggaccgcg gctacatcaa catggatatc gcctccaccc aggtatccat caccccggac aagaagcacg tctacatcac cgtcaacatc aacgagggtg agaagtacac catccgcgac gtgaagctga ccggcgacct gaaggtgccg gaagaggaag tgaagcgcct gctgctggta cagaagggcc aggtgttctc gcgcaaggtg atgaccacca cctccgacct aatcacccgt cgcctgggca acgagggcta caccttcgcc aacgtcaacg gcgtgccgga agcccatgac gacgacaaga ccgtctcggt gaccttcgtc gtcgacccgg gcaagcgcgc ctacgtcaac cgcatcaact tccgcggcaa caccaagacc gaagacgaag tgctgcgccg cgaaatgcgc cagatggaag gcggctgggc ctccacctac ctgatcgacc agtccaaggc ccgtctggag cgcctcggct acttcaagga agtcaacgtc gagaccccgg cggttcccgg caccgacgac caggtcgacg tcaactacag cgtcgaagag caaccgtccg gctcgatcac cgccagcgtc ggcttcgccc agagcgccgg cctgatcctc ggcggctcga tcagccagaa caacttcctg ggtaccggca acaaggtcag catcggcctg acccgcagcg aataccaaac ccgctacaac ttcggcttcg tcgaccccta ctggaccgtc gacggcgtca gcctcggcta caatgccttc taccggaaga ccgactacga cgaactcgac gtcgacgtcg ccagctactc ggtgaacagc ctgggcgccg gcatgagcat cggctacccg atcagcgaga cctcgcgcct gacctatggc ctgagcgtgc agcgcgacca gatcgacacc ggccgctaca ccgtggacga gatctacgac ttcctcgaca aggaaggcga caacttcacc aacttcaagg cttccatcgg ctggtccgaa tcgaccctga acaagggcgt actggccacc cgtggtcact cgcagagcct gaccctggaa accaccctgc cgggcagcga cctgtcgttc tacaagatcg actaccgtgg ccaggtcttc gccccgctga ccgacaacta caccatgcgc ttccacaccg agctgggcta tggcgacggc tacggatcca ccgagcgcct gccgttctac gagaactact acgccggcgg cttcaactcg gtgcggggct tcaaggacag cacgctgggc ccgcgcagta cgccctctgt ggctcgtaat ccagacggta cgcctatgaa gaaccaaggg cctgacagca agggccgtta caccgacccg gaccaggatc ccgaagcctt cggtggtaac atcctgatca ccggcggcgc cgagctgctg ttcccgctgc cgttcgtcaa ggaccagcgc cagttgcgta ccgtgctgtt ctgggacgtg ggtagcacct tcgataccga ctgcccgacc aagaccacca ccaactgcga cggcatcaag accgacaacc tcgccagttc cgtgggcgtc ggcctgacct ggatcaccgc gctgggcccg ctgagcttca gcctggcgac gccgatcaag aagccggaca acgcggaaac ccaggtcttc cagttctccc tgggccagac cttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat