PsaeA.18506.a

Putative adhesin

CENTER ID: PsaeA.18506.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18506.a.B2.GE35997 Pectin lyase domain 42 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4844
PGAT: pseudomonas:PA4625
RefSeq: NP_253315.1
UniProt: Q9HVG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVRPDSQRGK RTSSKAGREG RFVLAASSTL MFAIASINAA MAAPTGAQFN PNEIKISQQG KTTLIDQSTQ RAIINWKGFD VSADEAVRFN QPGVTSSTLN RVTAGQESVI AGRISAPGQV IIYNSNGVVF SGSAKVDVGS LITTTANISD EHFRQGKLIF DQPGNPDARI VNDGSISVAE KGLAAFVAPS VANNGVINAR LGTVAMAAGN AATIDLYGDG LVSIAVTDPV TRKPQDAQAL VSNGGAIQAD GGSVLITAEQ ASRVVDNAVN LSGVILARGT EVREGSVALV SKSGDIQIAG KIDVSGPKNG GDVLVSGQQV ALASTASIDA RGTAQGGSVR IGGDFQGRGE LPRAKNATLA KGASIDVSAT GKGNGGLAVV WSDGNTRMDG RILARGGAQG GNGGLVETSG KVNLSIADSA YVSVAAPYGN GGTWLLDPTT LRIVASGGTS GSVGGANGAS GDATVNASVV TGALAGGKVT LSASDRLSVE APLITSNLGG ASRGLELIAT GPAGAVDISA PILFRNGSLA IRAGGNINFL SGGTPQTSGI VDLGSGTLWM QTSTAGKISQ QAGTALIAAN LAGRAGSIDL ASWDNYAGNL ALQTFNGTLK YRQSNATGVT TSGTVFDPFI NQSMTGTAQN IVSSVGTRIL EANSVGTTGN YTLTADGNSE FDRLVFTALP YRRVSGSASF PTNDSSDYLV TNLRYQVNGS NVTATPNGGA PSGFTVAAGN GSVTTWTGNW GTSWGVKGFG GVIGVTDELQ YDVGTGLTEE LIFGLGGKTS RVDTRLDLFM REGAFNSFAE RAQVEMFKTT TTAGDILSRQ QTATLTANDA TRVYGDVNPT LTATMSGINA IDAYVNSQFN DLYQATASTT ATQASNVGQY AITGNANGSE YFSQRYQLVR QDGRLTVTPA QLIVSADAKT KVYGDADPTL TYQVSGLKNS DTAAGVLSGN LGRVAGENVG NYGILQGGLG LNTANYTLSY VGNDLRITPA QLNVIADAKT KVYGDLDPAL TYQVSGLKRG DTAGAVLNGG SLSRVAGENV GVYGINQGGL GLVSSNYTLN YQGNNLTITK ALLNVIADAK TKVYGDADPA LTYQVSGLKN GDTAGAVLNG GSLSRVAGEN VGVYGINQGG LGLLSANYDL SYQGNNLTIT KALLNVIADA KTKVYGDADP SLTYQVSGLK NGDTAGSILT GGLNRAAGEN VGVYGINQGD LALNSGNYDL SYQGNNLTIT KALLNVIADA KTKVYGDADP SLTYQVSGLK NGDTAGAVLN GGGLVRVSGE NVGNYAIQQG GLGLVSGNYD LAYQGNNLTI TKALLNVIAD AKTKVYGDAD PSLTYQVSGL KNGDSAGSIL TGGLNRAAGE NVGVYGINQG DLALNSGNYD LSYQGNNLTI TKALLNVIAD AKTKVYGDAD PSLTYQVSGL KNGDTAGAVL NGGGLVRVSG ENVGNYAIQQ GGLGLVSGNY DLAYQGNNLT ITKALLNVIA DAKTKVYGDA DPSLTYQVSG LKNGDTAGAV LNGGSLSRVA GENVGVYGIN QGDLALNSGN YDLSYQGNNL TITKALLNVI ADAKTKVYGD ADPSLTYQVS GLKNGDTAGA VLNGGGLVRV SGENVGNYAI QQGGLGLVSG NYDLAYQGNN LTITKALLNV IADAKTKVYG DADPSLTYQV SGLKNGDSAG SILTGGLNRA AGENVGVYGI NQGDLALNSG NYDLSYQGNN LTITKALLNV IADAKTKVYG DADPSLTYQV SGLKNGDTAG AVLNGGGLVR VSGENVGNYA IQQGGLGLVS GNYDLAYQGN NLTITKALLN VIADAKTKVY GDADPSLTYQ VSGLKNGDTA GAVLNGGSLS RVAGENVGVY GINQGGLGLV SGNYDLAYQG NNLTITKALL NVIADGKTKV YGDADPSLTY QVSGLKNGDS AGSILTGGLN RDAGENVGVY GINQGGLVLT SGNYDLAYQG NDLTITKALL NVFADAKSKQ VGTADPALTY QVSGLKNGDS AGQVLAGGLG RVGGEAVGQY DILQGGLALT SGNYQLNYQG NLLSILPLPV TPGDLGQLAA LSDLRELQKG RDPDTPGDAV YRTTTLENPF LENPFLRAYA LGMDVSDPNL LPATAAGPAE DASAKRVGQF TDRPLRAEAE SGAGCSNQSY LADYWSCFNK PLNF
NT Sequence
atggtccgtc cggatagcca gcgcggcaag cgcacctctt cgaaagctgg tagggaaggg cgtttcgtcc ttgccgccag cagcaccctc atgttcgcca tcgccagcat caacgcggcc atggcggcac ctaccggtgc ccagttcaac cccaacgaga tcaagatcag ccagcagggc aagaccacgc tgatcgacca gagcacccag cgcgcgatca tcaactggaa gggcttcgac gtctccgccg acgaggcggt gcgcttcaac cagcccggtg tgacctcgtc gaccctgaac cgggtcaccg caggccagga aagcgtgatc gccgggcgca tctccgcgcc cggccaggtg atcatctaca actccaacgg cgtggtcttc agcggctcgg ccaaggtcga cgtgggctcc ctgatcacca ccacggcgaa catcagcgac gaacacttcc gccagggcaa gctgatcttc gaccagcccg gaaaccccga cgcgcggatc gtcaacgacg gcagcatcag cgtcgcggag aaaggcctgg cggccttcgt cgcgccgtcc gtggccaaca acggcgtgat caacgcccgc ctcggcaccg tggcgatggc cgccggcaat gccgcgacca tcgacctcta cggcgatggc ctggtgtcga tcgccgtgac cgacccggtt acccgcaagc cgcaggatgc ccaggcgctg gtcagcaacg gtggcgcgat ccaggccgac ggcggtagcg tgctgatcac cgccgagcag gcttcccggg tggtcgacaa cgcggtcaac ctgtccggcg tgatccttgc ccgcggcacc gaggtgcgcg agggcagcgt ggcgctggta agcaagtccg gggacatcca gatcgccggc aagatcgacg tcagcggtcc gaagaacggc ggcgacgtgc tggtcagcgg gcagcaggtg gccctggcga gcaccgccag catcgacgcc cgcggcaccg cacagggcgg cagcgtgcgc atcggcggcg acttccaggg ccgcggcgag ctgccacggg cgaagaacgc tacgctggcc aagggcgcca gcatcgacgt ctcggctacc ggcaagggca atggcggcct ggcggtggtc tggtccgacg gcaatacccg catggacggg cggatcctcg cgcgcggcgg cgcccagggc ggcaacggcg gcctggtgga gacctccggc aaggtcaacc tgagcatcgc cgacagcgcc tacgtcagcg tcgccgcacc ctacggcaac ggtggcacct ggctgctcga cccgaccacc ctgcgcatcg tcgcctccgg cggcacctcc ggctcggtgg ggggcgccaa cggcgccagc ggcgacgcga cggtgaatgc ctcggtggtt accggtgcgc tggcaggcgg caaggtgacc ctcagcgcca gcgaccggct gtcggtggaa gcgccgctga tcaccagcaa cctgggcggc gccagtcgcg gcctggagct gatcgccacc ggacctgccg gtgcggtgga tatctctgcg ccgatcctgt tccgcaacgg cagcctggca atccgcgccg gcggcaacat caatttcctc agcggcggca cgccgcagac cagcggcatc gtcgacctgg gcagcggcac cctgtggatg cagaccagca ccgccggcaa gatcagccag caggccggta ccgcgctgat cgccgccaac ctcgccggcc gtgctggtag catcgacctg gcttcctggg acaactacgc cggcaacctg gcgctgcaga ccttcaacgg tacgctcaag taccggcaga gcaacgccac cggcgtgacc acgagcggca cggtgttcga tccgttcatc aaccagagca tgaccggcac cgcgcagaac atcgtctcca gcgtcggtac gcggattctc gaggccaaca gcgttggcac caccggcaac tacacgctga ctgccgacgg caacagcgag ttcgaccgcc tggtgttcac cgcgctgccc tatcggcggg tcagcggttc cgccagtttc cctaccaacg actcgtcgga ctacctggtg accaacctgc gctaccaggt caacggcagc aacgtcaccg ccaccccgaa cggcggtgcg cccagcgggt tcaccgtcgc cgccggcaac ggcagcgtca ccacctggac cggaaactgg ggcaccagtt ggggcgtcaa gggcttcggc ggcgtgatcg gcgtgaccga cgagctgcag tacgacgtcg gcaccggcct gaccgaggaa ctgatcttcg gcctcggcgg caagaccagc cgcgtcgata cccgcctgga cctgttcatg cgcgagggag cgttcaacag cttcgccgag cgcgcccagg tggagatgtt caagaccacc accactgccg gcgacatcct ttcccgccag cagaccgcca ccctgaccgc caatgacgct acccgcgtct acggtgatgt gaatccgact ctgacggcca cgatgtccgg catcaacgcc atcgacgcct acgtcaacag tcagttcaac gacctctacc aggcgaccgc cagcaccacg gcgacccagg cgtcgaatgt cgggcagtac gcgatcaccg gtaacgccaa tggcagcgag tatttcagcc agcgttacca actggtgcgc caggacggca ggctgaccgt cacgccggcg caattgatcg tcagcgccga cgccaagacc aaggtctatg gcgacgccga tccgaccctc acctaccagg tcagtggcct gaagaacagc gataccgccg ctggcgtgct gagcggcaac ctcggccggg tggccgggga gaacgtcggc aactacggga tactccaggg cgggctgggc ctcaacacgg ccaattacac cctcagctac gtcggcaacg acctgcgcat caccccggca caactgaacg tgatcgccga tgccaagacc aaggtctacg gcgacctgga tccggcgctg acctaccagg tctccggtct caagcgcggc gataccgccg gtgccgtgct caacggcggc agcctgagcc gcgtggctgg cgagaacgtc ggcgtgtacg gcatcaacca gggtggactg ggcctggtca gcagcaatta caccctgaac taccagggca ataacctgac catcaccaag gcactgctca atgtgatcgc cgatgccaag accaaggtct atggcgatgc cgatccggca ctgacctacc aggtgtccgg gctgaagaac ggcgacaccg ccggtgccgt gctcaacggc ggcagcctga gccgcgtggc tggcgagaat gtcggcgtgt acggcatcaa ccagggtgga ctgggcctgc tcagcgcgaa ctacgacctg agctaccagg gcaataacct gaccatcacc aaggcgctgc tgaatgtgat cgccgacgcc aagaccaagg tctacggcga tgccgatccg agcctgacct accaggtcag cggcctgaag aacggtgata ccgccggttc gatcctgacc ggtgggttga accgcgccgc aggcgagaac gtgggtgtgt atggcatcaa ccagggcgac ctggcattga acagcggtaa ctacgacctg agctaccagg gcaataacct gaccatcacc aaggcactgc tgaatgtgat cgccgacgcc aagaccaagg tctacggcga tgccgatccg agcctgacct accaggtcag cggcctgaag aatggcgata ccgctggcgc ggtgctcaat ggcggcggcc tggttcgtgt ctccggcgaa aacgtaggca actatgcgat tcagcagggt ggcctgggtc tggtcagcgg taactatgac ctggcttacc agggcaacaa cctgaccatc accaaggcac tgctgaatgt gatcgctgat gccaagacca aggtctacgg cgatgccgat ccgagcctga cctaccaggt cagcggcctg aagaacggcg acagcgccgg ttcgatcctg accggcgggc tgaaccgcgc cgcaggcgag aacgtgggtg tgtatggcat caaccagggc gacctggcat tgaacagcgg taactacgac ctgagctacc agggcaataa cctgaccatc accaaggcac tgctgaatgt gatcgccgat gccaagacca aggtctatgg cgatgccgac ccgagcctga cctaccaggt cagcggcctg aagaatggcg ataccgctgg cgcggtgctc aatggcggcg gcctggttcg tgtctccggc gaaaacgtag gcaactatgc gattcagcag ggtggcctgg gtctggtcag cggtaactat gacctggctt accagggcaa caacctgacc atcaccaagg cactgctgaa tgtgatcgcc gacgccaaga ccaaggtcta cggcgatgcc gatccgagcc tgacctacca ggtcagcggc ctgaagaacg gtgataccgc tggcgcggtg ctcaacggtg gcagcctgag ccgcgtggct ggcgagaatg tcggcgtgta cggcatcaac cagggcgacc tggcattgaa cagcggtaac tacgacctga gctaccaggg caataacctg accatcacca aggcgctgct gaatgtgatc gctgacgcca agaccaaggt ctacggcgat gccgatccga gcctgaccta ccaggtcagc ggcctgaaga atggcgatac cgctggcgcg gtgctcaatg gcggcggcct ggttcgtgtc tccggcgaaa acgtaggcaa ctatgcgatt cagcagggtg gcctgggtct ggtcagcggt aactatgacc tggcttacca gggcaacaac ctgaccatca ccaaggcact gctgaatgtg atcgctgatg ccaagaccaa ggtctacggc gatgccgatc cgagcctgac ctaccaggtc agcggcctga agaacggcga cagcgccggt tcgatcctga ccggcgggct gaaccgcgcc gcaggcgaga acgtgggtgt gtatggcatc aaccagggcg acctggcatt gaacagcggt aactacgacc tgagctacca gggcaataac ctgaccatca ccaaggcact gctgaatgtg atcgccgatg ccaagaccaa ggtctatggc gatgccgacc cgagcctgac ctaccaggtc agcggcctga agaatggcga taccgctggc gcggtgctca atggcggcgg cctggttcgt gtctccggcg aaaacgtagg caactatgcg attcagcagg gtggcctggg tctggtcagc ggtaactatg acctggctta ccagggcaac aacctgacca tcaccaaggc actgctgaat gtgatcgccg acgccaagac caaggtctac ggcgatgccg atccgagcct gacctaccag gtcagcggcc tgaagaacgg tgataccgct ggcgcggtgc tcaacggtgg cagcctgagc cgcgtggctg gcgagaatgt cggcgtgtac ggcatcaacc agggtggact gggcctggtc agcggtaact atgacctggc ttaccagggc aacaacctga ccatcaccaa ggcgctgctc aatgtgatcg ccgacggcaa gaccaaggtc tatggcgacg ctgacccgag cctgacctac caggtcagcg gcctgaagaa cggtgacagc gccggctcga tcctgaccgg cgggctgaac cgcgacgcag gcgagaacgt gggtgtgtat ggcatcaacc agggcggcct ggtgctgacc agtggcaact atgacctggc ctaccagggt aatgacctga ccatcaccaa ggcgctgctc aacgtattcg ccgatgccaa gagcaagcag gtcggcaccg ccgacccggc gctgacctat caggtttccg gcctgaagaa tggcgatagc gccggtcagg tgctcgccgg cgggctcggt cgggtgggcg gcgaagccgt aggccagtac gacatcctcc agggtgggct tgccctcacc agcggcaact accagctcaa ctaccagggc aacctgctga gcatcctgcc gctgccggtc acgccgggcg acctggggca actggcagcg ctctccgact tgcgcgaact gcagaagggg cgcgatccgg atacccccgg ggatgcggtc taccgcacca ccacgctgga aaacccgttc ctcgaaaacc cgttcctgcg cgcctacgct ctgggcatgg acgtcagcga tcctaacctg ctgccggcta ccgccgcggg tccggcggag gacgccagcg ccaagcgggt ggggcagttc accgatcgtc cgctcagggc agaggccgag agtggggcgg gttgctcgaa ccagagctac ctggccgact actggagctg cttcaacaag cctctgaatt tctaa
Details for PsaeA.18506.a.B2.GE35997
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMA APTGAQFNPN EIKISQQGKT TLIDQSTQRA IINWKGFDVS ADEAVRFNQP GVTSSTLNRV TAGQESVIAG RISAPGQVII YNSNGVVFSG SAKVDVGSLI TTTANISDEH FRQGKLIFDQ PGNPDARIVN DGSISVAEKG LAAFVAPSVA NNGVINARLG TVAMAAGNAA TIDLYGDGLV SIAVTDPVTR KPQDAQALVS NGGAIQADGG SVLITAEQAS RVVDNAVNLS GVILARGTEV REGSVALVSK SGDIQIAXKI DVSGPKNGGD VLVSGQXWPW RAPPASTXAA XHRAAACASA ATXXXXRXXR XXXATLXXXX HRXXAXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
tttgtttact ttaagaagga gatataccat ggctcaccac caccaccacc atatggcggc acctaccggt gcccagttca accccaacga gatcaagatc agccagcagg gcaagaccac gctgatcgac cagagcaccc agcgcgcgat catcaactgg aagggcttcg acgtctccgc cgacgaggcg gtgcgcttca accagcccgg tgtgacctcg tcgaccctga accgggtcac cgcaggccag gaaagcgtga tcgccgggcg catctccgcg cccggccagg tgatcatcta caactccaac ggcgtggtct tcagcggctc ggccaaggtc gacgtgggct ccctgatcac caccacggcg aacatcagcg acgaacactt ccgccagggc aagctgatct tcgaccagcc cggaaacccc gacgcgcgga tcgtcaacga cggcagcatc agcgtcgcgg agaaaggcct ggcggccttc gtcgcgccgt ccgtggccaa caacggcgtg atcaacgccc gcctcggcac cgtggcgatg gccgccggca atgccgcgac catcgacctc tacggcgatg gcctggtgtc gatcgccgtg accgacccgg ttacccgcaa gccgcaggat gcccaggcgc tggtcagcaa cggtggcgcg atccaggccg acggcggtag cgtgctgatc accgccgagc aggcttcccg ggtggtcgac aacgcggtca acctgtccgg cgtgatcctt gcccgcggca ccgaggtgcg cgagggcagc gtggcgctgg taagcaagtc cggggacatc cagatcgccn gcaagatcga cgtcagcggt ccgaagaacg gcggcgacgt gctggtcagc gggcagcngt ggccctggcg agcaccgcca gcatcgacgn ccgcggcanc gcacagggcg gcagcgtgcg catcngcggc nacttncnnn nnncnncgan ctncncgggn gaanaangct acnctngnna nggnncngca tcgacntntc gctncnnnnn nggnangnnn nnnnnnnnnt gncnangnnn nnncnncatg nnngnnnncn nnngnna
Expected Protein Sequence
MAHHHHHHMA APTGAQFNPN EIKISQQGKT TLIDQSTQRA IINWKGFDVS ADEAVRFNQP GVTSSTLNRV TAGQESVIAG RISAPGQVII YNSNGVVFSG SAKVDVGSLI TTTANISDEH FRQGKLIFDQ PGNPDARIVN DGSISVAEKG LAAFVAPSVA NNGVINARLG TVAMAAGNAA TIDLYGDGLV SIAVTDPVTR KPQDAQALVS NGGAIQADGG SVLITAEQAS RVVDNAVNLS GVILARGTEV REGSVALVSK SGDIQIAGKI DVSGPKNGGD VLVSGQQVAL ASTASIDARG TAQGGSVRIG GDFQGRGELP RAKNATLAKG ASIDVSATGK GNGGLAVVWS DGNTRMDGRI LARGGAQGGN GGLVETSGKV NLSIADSAYV SVAAPYGNGG TWLLDPTTLR IVASGGTSGS VGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg cacctaccgg tgcccagttc aaccccaacg agatcaagat cagccagcag ggcaagacca cgctgatcga ccagagcacc cagcgcgcga tcatcaactg gaagggcttc gacgtctccg ccgacgaggc ggtgcgcttc aaccagcccg gtgtgacctc gtcgaccctg aaccgggtca ccgcaggcca ggaaagcgtg atcgccgggc gcatctccgc gcccggccag gtgatcatct acaactccaa cggcgtggtc ttcagcggct cggccaaggt cgacgtgggc tccctgatca ccaccacggc gaacatcagc gacgaacact tccgccaggg caagctgatc ttcgaccagc ccggaaaccc cgacgcgcgg atcgtcaacg acggcagcat cagcgtcgcg gagaaaggcc tggcggcctt cgtcgcgccg tccgtggcca acaacggcgt gatcaacgcc cgcctcggca ccgtggcgat ggccgccggc aatgccgcga ccatcgacct ctacggcgat ggcctggtgt cgatcgccgt gaccgacccg gttacccgca agccgcagga tgcccaggcg ctggtcagca acggtggcgc gatccaggcc gacggcggta gcgtgctgat caccgccgag caggcttccc gggtggtcga caacgcggtc aacctgtccg gcgtgatcct tgcccgcggc accgaggtgc gcgagggcag cgtggcgctg gtaagcaagt ccggggacat ccagatcgcc ggcaagatcg acgtcagcgg tccgaagaac ggcggcgacg tgctggtcag cgggcagcag gtggccctgg cgagcaccgc cagcatcgac gcccgcggca ccgcacaggg cggcagcgtg cgcatcggcg gcgacttcca gggccgcggc gagctgccac gggcgaagaa cgctacgctg gccaagggcg ccagcatcga cgtctcggct accggcaagg gcaatggcgg cctggcggtg gtctggtccg acggcaatac ccgcatggac gggcggatcc tcgcgcgcgg cggcgcccag ggcggcaacg gcggcctggt ggagacctcc ggcaaggtca acctgagcat cgccgacagc gcctacgtca gcgtcgccgc accctacggc aacggtggca cctggctgct cgacccgacc accctgcgca tcgtcgcctc cggcggcacc tccggctcgg tggggggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat