SSGCID: Target Page

PsaeA.18465.a

3-deoxy-D-manno-octulosonic-acid (KDO) transferase

CENTER ID:	PsaeA.18465.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18465.a.B2.GE38534	mature protein		28	425

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18465.a.B2.PS02293	mature protein		28	425

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5228
PGAT:	pseudomonas:PA4988
RefSeq:	NP_253675.1
UniProt:	Q9HUH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRTLYTLLF HLGLPLVALR LWWRARQAPA YAKRIGERFS LSLPEVPPGG IWVHAVSVGE SIAAAPMVRA LLERHPQLPV TVTCMTPTGS ERIRALFGEQ VRHCYLPYDL PWAAARFLDR VRPRLAVIME TELWPNHIHA CAVRGIPVAL ANARLSERSA RGYARFAGLT RPMLAELSWI AVQTEAEAER FRRLGARPEC VSVTGSIKFD LRIDPQLPLA AAALREEWDA TARPLWIAAS THAGEDEIVL AAHRRLLETR PDALLILVPR HPERFAGVHE LCRREGFATV RRSGGEPVAR ATQVLLGDTM GELLFLYALA DIAFVGGSLV PNGGHNLLEP AALGKPVFAG PHLFNFLDIA AQLRDAGALL EVTDAGELCD GLARLWAQPE VATAMATAGE KVLRNNQGAL ERLLAGLARL LGRGG

NT Sequence

atgaaccgta ccctctatac cctgctgttc cacctcggct tgccgctggt ggcgttgcgc ctgtggtggc gcgcgcgcca ggcgccggcc tatgccaagc gcatcggcga gcgtttttcc ctgtccttgc ccgaggtgcc gcccggcggc atctgggtgc acgccgtctc ggtgggcgag agcatcgccg ccgcgccgat ggtgcgggcg ctgctggagc gccatccgca gctgccggtc accgtcacct gcatgacccc gaccggctcg gagcgcatcc gcgcattgtt cggcgagcag gtccggcact gctacctgcc ctacgacctg ccctgggcgg cggcgcgctt cctcgaccgg gtgcggccgc gcctggcggt gatcatggaa accgagctgt ggcccaacca tatccatgcc tgcgccgtgc gcgggatccc ggtggcgctg gccaatgcgc gcctgtccga gcgctcggcg cgtggctacg cgcgcttcgc cgggctgacc cggccgatgc tcgccgagct gtcctggatc gccgtgcaga ccgaggccga ggccgagcgc ttccgtcgtc tcggcgcgcg tcctgagtgc gtcagcgtga ccggctcgat caagttcgac ctgcgcatcg acccgcagct gccgctggcc gctgccgccc tgcgcgagga atgggacgcg acggcgcgac cgctgtggat cgccgccagc acccatgccg gcgaggacga gatcgtcctg gcagcccacc ggcgcctgct ggaaacgcgt cccgacgcgt tgctgatcct ggtgccgcgc catccggaac gcttcgccgg cgtccacgag ctatgccgcc gcgaaggctt cgccacggtc cggcgctccg gcggcgagcc ggtggcacgc gcgacccagg tgctgctcgg cgataccatg ggcgagctgc tgttcctcta tgccctggcc gacatcgctt tcgtcggcgg cagcctggtg cccaacggcg ggcacaacct gctggagccg gcggccctgg gcaagccggt gttcgccggt ccgcacctgt tcaacttcct cgacatcgcc gcgcaattgc gcgacgccgg ggcgctgctc gaggtgacgg atgccggcga gctgtgcgac ggcctcgcgc gcctgtgggc gcagccggag gtcgcgacgg ccatggccac tgccggagag aaggtgctgc gcaacaacca gggcgccctg gagcggctgc tggcggggtt ggcgcggttg ctcgggcgcg gcgggtag

Details for PsaeA.18465.a.B2.GE38534

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA PAYAKRIGER FSLSLPEVPP GGIWVHAVSV GESIAAAPMV RALLERHPQL PVTVTCMTPT GSERIRALFG EQVRHCYLPY DLPWAAARFL DRVRPRLAVI METELWPNHI HACAVRGIPV ALANARLSER SARGYARFAG LTRPMLAELS WIAVQTEAEA ERFRRLGARP ECVSVTGSIK FDLRIDPQLP LAAAALREEW DATARPLWIA ASTHAGEDEI VLAAHRRLLE TRPDALLILV PRHPERFAGV HELCRREGFA TVRRSGGEPV ARATQVLLGD TMGELLFLYA LADIAFVGGS LVPNGGHNLL EPAALGKPVF AGPHLFNFLD IAAQLRDAGA LLEVTDAGEL CDGLARLWAQ PEVATAMATA GEKVLRNNQG ALERLLAG

Validated NT Sequence

accccgccag cagccgctcc agggcgccct ggttgttgcg cagcaccttc tctccggcag tggccatggc cgtcgcgacc tccggctgcg cccacaggcg cgcgaggccg tcgcacagct cgccggcatc cgtcacctcg agcagcgccc cggcgtcgcg caattgcgcg gcgatgtcga ggaagttgaa caggtgcgga ccggcgaaca ccggcttgcc cagggccgcc ggctccagca ggttgtgccc gccgttgggc accaggctgc cgccgacgaa agcgatgtcg gccagggcat agaggaacag cagctcgccc atggtatcgc cgagcagcac ctgggtcgcg cgtgccaccg gctcgccgcc ggagcgccgg accgtggcga agccttcgcg gcggcatagc tcgtggacgc cggcgaagcg ttccggatgg cgcggcacca ggatcagcaa cgcgtcggga cgcgtttcca gcaggcgccg gtgggctgcc aggacgatct cgtcctcgcc ggcatgggtg ctggcggcga tccacagcgg tcgcgccgtc gcgtcccatt cctcgcgcag ggcggcagcg gccagcggca gctgcgggtc gatgcgcagg tcgaacttga tcgagccggt cacgctgacg cactcaggac gcgcgccgag acgacggaag cgctcggcct cggcctcggt ctgcacggcg atccaggaca gctcggcgag catcggccgg gtcagcccgg cgaagcgcgc gtagccacgc gccgagcgct cggacaggcg cgcattggcc agcgccaccg ggatcccgcg cacggcgcag gcatggatat ggttgggcca cagctcggtt tccatgatca ccgccaggcg cggccgcacc cggtcgagga agcgcgccgc cgcccagggc aggtcgtagg gcaggtagca gtgccggacc tgctcgccga acaatgcgcg gatgcgctcc gagccggtcg gggtcatgca ggtgacggtg accggcagct gcggatggcg ctccagcagc gcccgcacca tcggcgcggc ggcgatgctc tcgcccaccg agacggcgtg cacccagatg ccgccgggcg gcacctcggg caaggacagg gaaaaacgct cgccgatgcg cttggcatag gccggcgcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHAP AYAKRIGERF SLSLPEVPPG GIWVHAVSVG ESIAAAPMVR ALLERHPQLP VTVTCMTPTG SERIRALFGE QVRHCYLPYD LPWAAARFLD RVRPRLAVIM ETELWPNHIH ACAVRGIPVA LANARLSERS ARGYARFAGL TRPMLAELSW IAVQTEAEAE RFRRLGARPE CVSVTGSIKF DLRIDPQLPL AAAALREEWD ATARPLWIAA STHAGEDEIV LAAHRRLLET RPDALLILVP RHPERFAGVH ELCRREGFAT VRRSGGEPVA RATQVLLGDT MGELLFLYAL ADIAFVGGSL VPNGGHNLLE PAALGKPVFA GPHLFNFLDI AAQLRDAGAL LEVTDAGELC DGLARLWAQP EVATAMATAG EKVLRNNQGA LERLLAGLAR LLGRGG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgccgg cctatgccaa gcgcatcggc gagcgttttt ccctgtcctt gcccgaggtg ccgcccggcg gcatctgggt gcacgccgtc tcggtgggcg agagcatcgc cgccgcgccg atggtgcggg cgctgctgga gcgccatccg cagctgccgg tcaccgtcac ctgcatgacc ccgaccggct cggagcgcat ccgcgcattg ttcggcgagc aggtccggca ctgctacctg ccctacgacc tgccctgggc ggcggcgcgc ttcctcgacc gggtgcggcc gcgcctggcg gtgatcatgg aaaccgagct gtggcccaac catatccatg cctgcgccgt gcgcgggatc ccggtggcgc tggccaatgc gcgcctgtcc gagcgctcgg cgcgtggcta cgcgcgcttc gccgggctga cccggccgat gctcgccgag ctgtcctgga tcgccgtgca gaccgaggcc gaggccgagc gcttccgtcg tctcggcgcg cgtcctgagt gcgtcagcgt gaccggctcg atcaagttcg acctgcgcat cgacccgcag ctgccgctgg ccgctgccgc cctgcgcgag gaatgggacg cgacggcgcg accgctgtgg atcgccgcca gcacccatgc cggcgaggac gagatcgtcc tggcagccca ccggcgcctg ctggaaacgc gtcccgacgc gttgctgatc ctggtgccgc gccatccgga acgcttcgcc ggcgtccacg agctatgccg ccgcgaaggc ttcgccacgg tccggcgctc cggcggcgag ccggtggcac gcgcgaccca ggtgctgctc ggcgatacca tgggcgagct gctgttcctc tatgccctgg ccgacatcgc tttcgtcggc ggcagcctgg tgcccaacgg cgggcacaac ctgctggagc cggcggccct gggcaagccg gtgttcgccg gtccgcacct gttcaacttc ctcgacatcg ccgcgcaatt gcgcgacgcc ggggcgctgc tcgaggtgac ggatgccggc gagctgtgcg acggcctcgc gcgcctgtgg gcgcagccgg aggtcgcgac ggccatggcc actgccggag agaaggtgct gcgcaacaac cagggcgccc tggagcggct gctggcgggg ttggcgcggt tgctcgggcg cggcgggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for PsaeA.18465.a.B2.PS02293

PURIFICATION DATe:	1/7/2015
CONCENTRATION:	12.96mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGCCGG CCTATGCCAA GCGCATCGGC GAGCGTTTTT CCCTGTCCTT GCCCGAGGTG CCGCCCGGCG GCATCTGGGT GCACGCCGTC TCGGTGGGCG AGAGCATCGC CGCCGCGCCG ATGGTGCGGG CGCTGCTGGA GCGCCATCCG CAGCTGCCGG TCACCGTCAC CTGCATGACC CCGACCGGCT CGGAGCGCAT CCGCGCATTG TTCGGCGAGC AGGTCCGGCA CTGCTACCTG CCCTACGACC TGCCCTGGGC GGCGGCGCGC TTCCTCGACC GGGTGCGGCC GCGCCTGGCG GTGATCATGG AAACCGAGCT GTGGCCCAAC CATATCCATG CCTGCGCCGT GCGCGGGATC CCGGTGGCGC TGGCCAATGC GCGCCTGTCC GAGCGCTCGG CGCGTGGCTA CGCGCGCTTC GCCGGGCTGA CCCGGCCGAT GCTCGCCGAG CTGTCCTGGA TCGCCGTGCA GACCGAGGCC GAGGCCGAGC GCTTCCGTCG TCTCGGCGCG CGTCCTGAGT GCGTCAGCGT GACCGGCTCG ATCAAGTTCG ACCTGCGCAT CGACCCGCAG CTGCCGCTGG CCGCTGCCGC CCTGCGCGAG GAATGGGACG CGACGGCGCG ACCGCTGTGG ATCGCCGCCA GCACCCATGC CGGCGAGGAC GAGATCGTCC TGGCAGCCCA CCGGCGCCTG CTGGAAACGC GTCCCGACGC GTTGCTGATC CTGGTGCCGC GCCATCCGGA ACGCTTCGCC GGCGTCCACG AGCTATGCCG CCGCGAAGGC TTCGCCACGG TCCGGCGCTC CGGCGGCGAG CCGGTGGCAC GCGCGACCCA GGTGCTGCTC GGCGATACCA TGGGCGAGCT GCTGTTCCTC TATGCCCTGG CCGACATCGC TTTCGTCGGC GGCAGCCTGG TGCCCAACGG CGGGCACAAC CTGCTGGAGC CGGCGGCCCT GGGCAAGCCG GTGTTCGCCG GTCCGCACCT GTTCAACTTC CTCGACATCG CCGCGCAATT GCGCGACGCC GGGGCGCTGC TCGAGGTGAC GGATGCCGGC GAGCTGTGCG ACGGCCTCGC GCGCCTGTGG GCGCAGCCGG AGGTCGCGAC GGCCATGGCC ACTGCCGGAG AGAAGGTGCT GCGCAACAAC CAGGGCGCCC TGGAGCGGCT GCTGGCGGGG TTGGCGCGGT TGCTCGGGCG CGGCGGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT