SSGCID: Target Page

PsaeA.18385.a

Quorum threshold expression element

CENTER ID:	PsaeA.18385.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.18385.a.B1.GE35996	full length		1	190

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.2714
RefSeq:	NP_251283.1
UniProt:	Q9I0P4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLIHFCPRLL TPAGFDLPCE LIDIRIKEFD LHLLGGRDVV ARHPLPDKRY HVACRKSGCK AVNGLLVDVE KHVPLFTVDT RWSIDAEVVL RHRVEYVVLD AEHDAVSDYM LLWCDEVPNY FLGQSTPAMQ VPLMELMRGN ALQTERQDVF RLPTLRSERL RQGNADANQH LPSREQAFHV KAEQISYGLA

NT Sequence

atgcttatcc atttctgccc caggctgctg acgcctgcgg ggttcgacct gccgtgcgag ttgatcgaca tccgcatcaa ggagttcgac ctgcacctgc ttggcgggcg cgacgtggtg gcgcgtcacc ccctccccga caagcgctac cacgtggcct gccgcaagtc cggctgcaag gcggtgaatg gcctgctggt ggacgtggaa aagcacgttc cgctgttcac cgtggacacc cgctggagca tcgacgccga agtggtcctg cgtcaccgcg tcgagtacgt ggtgctcgac gccgagcacg atgcggtgag cgactacatg ctgctgtggt gcgacgaggt gcccaactac ttcctcggcc agagcacccc ggccatgcag gttccgttga tggagctgat gcgcggcaat gcgttgcaga ccgagcggca ggatgtgttc cgcctgccga cgctgaggtc cgaacgcctg cgccagggca acgccgatgc caaccagcat cttccgagcc gtgagcaggc gttccacgtc aaggccgagc agatcagcta tgggctggcc tga

Details for PsaeA.18385.a.B1.GE35996

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	22kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IHFCPRLLTP AGFDLPCELI DIRIKEFDLH LLGGRDVVAR HPLPDKRYHV ACRKSGCKAV NGLLVDVEKH VPLFTVDTRW SIDAEVVLRH RVEYVVLDAE HDAVSDYMLL WCDEVPNYFL GQSTPAMQVP LMELMRGNAL QTERQDVFRL PTLRSERLRQ GNADANQHLP SREQAFHVKA EQISYGLA

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgctt atccatttct gccccaggct gctgacgcct gcggggttcg acctgccgtg cgagttgatc gacatccgca tcaaggagtt cgacctgcac ctgcttggcg ggcgcgacgt ggtggcgcgt caccccctcc ccgacaagcg ctaccacgtg gcctgccgca agtccggctg caaggcggtg aatggcctgc tggtggacgt ggaaaagcac gttccgctgt tcaccgtgga cacccgctgg agcatcgacg ccgaagtggt cctgcgtcac cgcgtcgagt acgtggtgct cgacgccgag cacgatgcgg tgagcgacta catgctgctg tggtgcgacg aggtgcccaa ctacttcctc ggccagagca ccccggccat gcaggttccg ttgatggagc tgatgcgcgg caatgcgttg cagaccgagc ggcaggatgt gttccgcctg ccgacgctga ggtccgaacg cctgcgccag ggcaacgccg atgccaacca gcatcttccg agccgtgagc aggcgttcca cgtcaaggcc gagcagatca gctatgggct ggcctaagtg agtaagatag gatccggctg ctnnnnnncc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctta tccatttctg ccccaggctg ctgacgcctg cggggttcga cctgccgtgc gagttgatcg acatccgcat caaggagttc gacctgcacc tgcttggcgg gcgcgacgtg gtggcgcgtc accccctccc cgacaagcgc taccacgtgg cctgccgcaa gtccggctgc aaggcggtga atggcctgct ggtggacgtg gaaaagcacg ttccgctgtt caccgtggac acccgctgga gcatcgacgc cgaagtggtc ctgcgtcacc gcgtcgagta cgtggtgctc gacgccgagc acgatgcggt gagcgactac atgctgctgt ggtgcgacga ggtgcccaac tacttcctcg gccagagcac cccggccatg caggttccgt tgatggagct gatgcgcggc aatgcgttgc agaccgagcg gcaggatgtg ttccgcctgc cgacgctgag gtccgaacgc ctgcgccagg gcaacgccga tgccaaccag catcttccga gccgtgagca ggcgttccac gtcaaggccg agcagatcag ctatgggctg gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat