PsaeA.18222.a
Glutamine--tRNA ligase (EC 6.1.1.18) (Glutaminyl-tRNA synthetase) (GlnRS)
| CENTER ID: | PsaeA.18222.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.18222.a.B1.GE38532 | full length | 1 | 556 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.18222.a.B1.PW37623 | full length | 1 | 556 |
Structures
5BNZ
DEPOSITED: 5/26/2015
DETERMINATION: XRay
CLONE: PsaeA.18222.a.B1.GE38532
PROTEIN: PsaeA.18222.a.B1.PW37623
Publications by SSGCID
Glutaminyl-tRNA synthetase from Pseudomonas aeruginosa: Characterization, Structure, and Development as a Screening Platform
Abendroth J, Dranow DM, Bullard JM, Escamilla Y, Hughes CA, Balboa S, Dean F
Protein Sci. - 2019
volume 29, issue 4, pages 905-918
PMID: 31833153; PMCID: PMC7096721
PDB:
5BNZ
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.1861 |
| PGAT: | pseudomonas:PA1794 |
| RefSeq: | NP_250485.1 |
| UniProt: | Q9I2U8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKPETTAAP NFLRQIVQAD LDAGKHAKIV TRFPPEPNGY LHIGHAKSIC LNFGLAQEFA GDCHLRFDDT NPAKEDQEYI DAIEADIKWL GFQWSGEVCY ASNYFDQLHA WAVELIKAGK AFVCDLGPEE MREYRGTLTE PGRNSPYRDR SVEENLDLFA RMKAGEFPDG ARSLRAKIDM GSPNMNLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP LYEWFLANLP VPAQPRQYEF SRLNLNYTVT SKRKLKQLVD EGHVSGWDDP RMSTLSGYRR RGYTPESIRN FCEMIGVNRA SGVVDIGMLE FSIRDHLDAT APRAMCVLKP LKVVITNYPE GQVENLELPR HPKEDMGVRV LPFGRELFID AGDFEEVPPA GYKRLIPGGE VRLRGSYVIR ADEAIKDADG NIVELRCSYD PDTLGKNPEG RKVKGVIHWV PAEGSVECEV RLYDRLFRSA NPEKAEEGGS FLDNINADSL QVLAGCRAEP SLGQANPEDR FQFEREGYFV ADLKDSRPGK PVFNRTVTLR DSWGQG
NT Sequence
atgagcaagc cagagaccac cgccgccccg aacttcctgc gccagatcgt tcaagccgac ctggatgccg gcaagcacgc gaagatcgtc acccgtttcc ccccggagcc caacggttac ctgcacatcg gccacgccaa gtcgatctgc ctgaacttcg gcctggccca ggaattcgct ggcgattgcc atctgcgctt cgatgacacc aacccggcca aggaagacca ggagtacatc gatgccatcg aggccgacat caagtggctg ggcttccagt ggtccggcga ggtctgctat gcctccaact atttcgacca gctgcatgcc tgggccgtcg agctgatcaa ggccggcaag gcgttcgtct gcgacctcgg tcccgaggaa atgcgcgaat accgcggcac cctcaccgag ccgggccgca acagccctta ccgtgaccgt tcggtggagg agaacctcga cctgttcgcc cgcatgaagg ccggtgagtt ccccgacggc gcccgttcgc tgcgggcgaa gatcgacatg ggttcgccga acatgaacct gcgcgatccg atcctgtacc gcatccgcca tgcgcatcac caccagaccg gcgacaagtg gtgcatctac ccgagctacg acttcaccca cggtcagtcg gacgccatcg agggcatcac ccattcgatc tgcaccctgg aattcgaaga ccatcgtccg ctctacgagt ggttcctggc caacctgccg gtacctgcgc aaccgcgcca gtacgaattc tcccggctca acctgaacta caccgtgacc agcaagcgca agctcaagca attggtcgac gaaggccatg tcagcggctg ggacgatccg cgcatgtcga ccctgtccgg ctatcgccgc cgcggctaca ccccggagtc gatccgcaac ttctgcgaga tgatcggggt gaaccgcgcc agcggcgtgg tcgacatcgg catgctcgag ttcagcatcc gcgaccacct ggacgccacc gcgccgcgtg ccatgtgcgt gctgaaaccg ctcaaggtgg tgatcaccaa ctaccccgaa gggcaggtgg agaacctcga gttgccgcgt catccgaagg aagacatggg agtgcgcgta ctgccgttcg gtcgcgagct gttcatcgat gccggtgact tcgaggaagt cccgccggcc ggctacaagc gcctgatccc cggtggcgag gtgcgtctgc gcggcagcta cgtgatccgt gccgacgaag cgatcaagga tgccgacggc aatatcgtcg aactgcgctg cagctacgat ccggacaccc tgggcaagaa cccggaaggc cgcaaggtca agggcgtgat ccactgggtg ccggccgagg gcagcgtcga gtgcgaagtg cgcctgtacg atcggctgtt ccgctcggcc aatccggaga aggcggaaga gggcggcagc ttcctcgaca acatcaacgc cgactccctg caggtactgg ccggctgtcg cgccgagccg tcgctgggcc aggcgaaccc ggaggatcgc ttccagttcg agcgcgaagg ctatttcgtc gccgacctga aggactccag gccgggcaag ccggtgttca accgtaccgt caccctgcgt gactcctggg gacagggctg a
Details for PsaeA.18222.a.B1.GE38532
| HARVESTED ON: | 10/9/2014 |
| SEQUENCED ON: | 11/24/2014 |
| EXPECTED MW: | 64kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 71 |
| PERCENT COVERAGE: | 57 |
Validated AA Sequence
MAHHHHHHMS KPETTAAPNF LRQIVQADLD AGKHAKIVTR FPPEPNGYLH IGHAKSICLN FGLAQEFAGD CHLRFDDTNP AKEDQEYIDA IEADIKWLGF QWSGEVCYAS NYFDQLHAWA VELIKAGKAF VCDLGPEEMR EYRGTLTEPG RNSPYRDRSV EENLDLFARM KAGEFPDGAR SLRAKIDMGS PNMNLRDPIL YRIRXAXXHQ TGDKWCIYPX XDXTHGQXXA IEGXXHXXXX LXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XAXXXXXXXX XXXXXXXTIX XCXXCPXIAA XXTPRXXSAX XAR
Validated NT Sequence
gtgacggtac ggttgaacac cggnntgccc ggcctnnngt ccttcaggtc ggcgannnna tagccttcgc gctcgaactg gaagcgatcc tccgggttcg cctggcccag cgacggctcg gcgcgacagc cggccagtac ctgcagggag tcggcgttga tgttgtcgag gaagctgccg ccctcttccg ccttctccgg attggccgag cggaacagcc gatcgtacag gcgcacttcg cactcgacgc tgccctcggc cggcacccag tggatcacgc ccttgacctt gcggccttcc gggttcttgc ccagggtgtc cggatcgtag ctgcagcgca gttcgacgat attgccgtcg gcatccttga tcgcttcgtc ggcacggatc acgtagctgc cgcgcagacg cacctcgcca ccggggatca ggcgcttgta gccggccggc gggacttcct cgaagtcacc ggcatcgatg aacagctcgc gaccgaacgg cagtacgcgc actcccatgt cttccttcgg atgacgcggc aactcgaggt tctccacctg cccttcgggg tagttggtga tcaccacctt gagcggtttc agcacgcaca tggcacgcgg cgcggtggcg tccaggtggt cgcggatgct gaactcgagc atgccgatgt cgaccacgcc gctggcgcgg ttcaccccga tcatctcgcn nnnnttgcgg atcnnctccg gggtgtannn ncggcggcga tagncggaca nnnnnnacan nnnnggatcg tcnnnnnnnn tnnnannnnn nnnnnnnnnn nnnnnctnna nnnnnnngct nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnntnnnn nnnnnnntcc aggnnncnnn nnnaatggnn nntgccctcg atggcgnnnn nctgaccgtg ggtnnngtcn tnnnnngggt agatgcacca cttgtcgccg gtctggtggn nntgcgcnnn gcggatgcgg tacaggatcg gatcgcgcag gttcatgttc ggcgaaccca tgtcgatctt cgcccgcagc gaacgggcgc cgtcggggaa ctcaccggcc ttcatgcggg cgaacaggtc gaggttctcc tccaccgaac ggtcacggta agggctgttg cggcccggct cggtgagggt gccgcggtat tcgcgcattt cctcgggacc gaggtcgcag acgaacgcct tgccggcctt gatcagctcg acggcccagg catgcagctg gtcgaaatag ttggaggcat agcagacctc gccggaccac tggaagccca gccacttgat gtcggcctcg atggcatcga tgtactcctg gtcttccttg gccgggttgg tgtcatcgaa gcgcagatgg caatcgccag cgaattcctg ggccaggccg aagttcaggc agatcgactt ggcgtggccg atgtgcaggt aaccgttggg ctccgggggg aaacgggtga cgatcttcgc gtgcttgccg gcatccaggt cggcttgaac gatctggcgc aggaagttcg gggcggcggt ggtctctggc ttgctcatat ggtggtggtg gtggtgagcc atggtatatc t
Expected Protein Sequence
MAHHHHHHMS KPETTAAPNF LRQIVQADLD AGKHAKIVTR FPPEPNGYLH IGHAKSICLN FGLAQEFAGD CHLRFDDTNP AKEDQEYIDA IEADIKWLGF QWSGEVCYAS NYFDQLHAWA VELIKAGKAF VCDLGPEEMR EYRGTLTEPG RNSPYRDRSV EENLDLFARM KAGEFPDGAR SLRAKIDMGS PNMNLRDPIL YRIRHAHHHQ TGDKWCIYPS YDFTHGQSDA IEGITHSICT LEFEDHRPLY EWFLANLPVP AQPRQYEFSR LNLNYTVTSK RKLKQLVDEG HVSGWDDPRM STLSGYRRRG YTPESIRNFC EMIGVNRASG VVDIGMLEFS IRDHLDATAP RAMCVLKPLK VVITNYPEGQ VENLELPRHP KEDMGVRVLP FGRELFIDAG DFEEVPPAGY KRLIPGGEVR LRGSYVIRAD EAIKDADGNI VELRCSYDPD TLGKNPEGRK VKGVIHWVPA EGSVECEVRL YDRLFRSANP EKAEEGGSFL DNINADSLQV LAGCRAEPSL GQANPEDRFQ FEREGYFVAD LKDSRPGKPV FNRTVTLRDS WGQG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agccagagac caccgccgcc ccgaacttcc tgcgccagat cgttcaagcc gacctggatg ccggcaagca cgcgaagatc gtcacccgtt tccccccgga gcccaacggt tacctgcaca tcggccacgc caagtcgatc tgcctgaact tcggcctggc ccaggaattc gctggcgatt gccatctgcg cttcgatgac accaacccgg ccaaggaaga ccaggagtac atcgatgcca tcgaggccga catcaagtgg ctgggcttcc agtggtccgg cgaggtctgc tatgcctcca actatttcga ccagctgcat gcctgggccg tcgagctgat caaggccggc aaggcgttcg tctgcgacct cggtcccgag gaaatgcgcg aataccgcgg caccctcacc gagccgggcc gcaacagccc ttaccgtgac cgttcggtgg aggagaacct cgacctgttc gcccgcatga aggccggtga gttccccgac ggcgcccgtt cgctgcgggc gaagatcgac atgggttcgc cgaacatgaa cctgcgcgat ccgatcctgt accgcatccg ccatgcgcat caccaccaga ccggcgacaa gtggtgcatc tacccgagct acgacttcac ccacggtcag tcggacgcca tcgagggcat cacccattcg atctgcaccc tggaattcga agaccatcgt ccgctctacg agtggttcct ggccaacctg ccggtacctg cgcaaccgcg ccagtacgaa ttctcccggc tcaacctgaa ctacaccgtg accagcaagc gcaagctcaa gcaattggtc gacgaaggcc atgtcagcgg ctgggacgat ccgcgcatgt cgaccctgtc cggctatcgc cgccgcggct acaccccgga gtcgatccgc aacttctgcg agatgatcgg ggtgaaccgc gccagcggcg tggtcgacat cggcatgctc gagttcagca tccgcgacca cctggacgcc accgcgccgc gtgccatgtg cgtgctgaaa ccgctcaagg tggtgatcac caactacccc gaagggcagg tggagaacct cgagttgccg cgtcatccga aggaagacat gggagtgcgc gtactgccgt tcggtcgcga gctgttcatc gatgccggtg acttcgagga agtcccgccg gccggctaca agcgcctgat ccccggtggc gaggtgcgtc tgcgcggcag ctacgtgatc cgtgccgacg aagcgatcaa ggatgccgac ggcaatatcg tcgaactgcg ctgcagctac gatccggaca ccctgggcaa gaacccggaa ggccgcaagg tcaagggcgt gatccactgg gtgccggccg agggcagcgt cgagtgcgaa gtgcgcctgt acgatcggct gttccgctcg gccaatccgg agaaggcgga agagggcggc agcttcctcg acaacatcaa cgccgactcc ctgcaggtac tggccggctg tcgcgccgag ccgtcgctgg gccaggcgaa cccggaggat cgcttccagt tcgagcgcga aggctatttc gtcgccgacc tgaaggactc caggccgggc aagccggtgt tcaaccgtac cgtcaccctg cgtgactcct ggggacaggg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PsaeA.18222.a.B1.PW37623
| PURIFICATION DATe: | 1/5/2015 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 71 |
| PERCENT COVERAGE: | 57 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KPETTAAPNF LRQIVQADLD AGKHAKIVTR FPPEPNGYLH IGHAKSICLN FGLAQEFAGD CHLRFDDTNP AKEDQEYIDA IEADIKWLGF QWSGEVCYAS NYFDQLHAWA VELIKAGKAF VCDLGPEEMR EYRGTLTEPG RNSPYRDRSV EENLDLFARM KAGEFPDGAR SLRAKIDMGS PNMNLRDPIL YRIRXAXXHQ TGDKWCIYPX XDXTHGQXXA IEGXXHXXXX LXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XAXXXXXXXX XXXXXXXTIX XCXXCPXIAA XXTPRXXSAX XAR
Validated NT Sequence
gtgacggtac ggttgaacac cggnntgccc ggcctnnngt ccttcaggtc ggcgannnna tagccttcgc gctcgaactg gaagcgatcc tccgggttcg cctggcccag cgacggctcg gcgcgacagc cggccagtac ctgcagggag tcggcgttga tgttgtcgag gaagctgccg ccctcttccg ccttctccgg attggccgag cggaacagcc gatcgtacag gcgcacttcg cactcgacgc tgccctcggc cggcacccag tggatcacgc ccttgacctt gcggccttcc gggttcttgc ccagggtgtc cggatcgtag ctgcagcgca gttcgacgat attgccgtcg gcatccttga tcgcttcgtc ggcacggatc acgtagctgc cgcgcagacg cacctcgcca ccggggatca ggcgcttgta gccggccggc gggacttcct cgaagtcacc ggcatcgatg aacagctcgc gaccgaacgg cagtacgcgc actcccatgt cttccttcgg atgacgcggc aactcgaggt tctccacctg cccttcgggg tagttggtga tcaccacctt gagcggtttc agcacgcaca tggcacgcgg cgcggtggcg tccaggtggt cgcggatgct gaactcgagc atgccgatgt cgaccacgcc gctggcgcgg ttcaccccga tcatctcgcn nnnnttgcgg atcnnctccg gggtgtannn ncggcggcga tagncggaca nnnnnnacan nnnnggatcg tcnnnnnnnn tnnnannnnn nnnnnnnnnn nnnnnctnna nnnnnnngct nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnntnnnn nnnnnnntcc aggnnncnnn nnnaatggnn nntgccctcg atggcgnnnn nctgaccgtg ggtnnngtcn tnnnnngggt agatgcacca cttgtcgccg gtctggtggn nntgcgcnnn gcggatgcgg tacaggatcg gatcgcgcag gttcatgttc ggcgaaccca tgtcgatctt cgcccgcagc gaacgggcgc cgtcggggaa ctcaccggcc ttcatgcggg cgaacaggtc gaggttctcc tccaccgaac ggtcacggta agggctgttg cggcccggct cggtgagggt gccgcggtat tcgcgcattt cctcgggacc gaggtcgcag acgaacgcct tgccggcctt gatcagctcg acggcccagg catgcagctg gtcgaaatag ttggaggcat agcagacctc gccggaccac tggaagccca gccacttgat gtcggcctcg atggcatcga tgtactcctg gtcttccttg gccgggttgg tgtcatcgaa gcgcagatgg caatcgccag cgaattcctg ggccaggccg aagttcaggc agatcgactt ggcgtggccg atgtgcaggt aaccgttggg ctccgggggg aaacgggtga cgatcttcgc gtgcttgccg gcatccaggt cggcttgaac gatctggcgc aggaagttcg gggcggcggt ggtctctggc ttgctcatat ggtggtggtg gtggtgagcc atggtatatc t
Expressed Protein Sequence
MAHHHHHHMS KPETTAAPNF LRQIVQADLD AGKHAKIVTR FPPEPNGYLH IGHAKSICLN FGLAQEFAGD CHLRFDDTNP AKEDQEYIDA IEADIKWLGF QWSGEVCYAS NYFDQLHAWA VELIKAGKAF VCDLGPEEMR EYRGTLTEPG RNSPYRDRSV EENLDLFARM KAGEFPDGAR SLRAKIDMGS PNMNLRDPIL YRIRHAHHHQ TGDKWCIYPS YDFTHGQSDA IEGITHSICT LEFEDHRPLY EWFLANLPVP AQPRQYEFSR LNLNYTVTSK RKLKQLVDEG HVSGWDDPRM STLSGYRRRG YTPESIRNFC EMIGVNRASG VVDIGMLEFS IRDHLDATAP RAMCVLKPLK VVITNYPEGQ VENLELPRHP KEDMGVRVLP FGRELFIDAG DFEEVPPAGY KRLIPGGEVR LRGSYVIRAD EAIKDADGNI VELRCSYDPD TLGKNPEGRK VKGVIHWVPA EGSVECEVRL YDRLFRSANP EKAEEGGSFL DNINADSLQV LAGCRAEPSL GQANPEDRFQ FEREGYFVAD LKDSRPGKPV FNRTVTLRDS WGQG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AGCCAGAGAC CACCGCCGCC CCGAACTTCC TGCGCCAGAT CGTTCAAGCC GACCTGGATG CCGGCAAGCA CGCGAAGATC GTCACCCGTT TCCCCCCGGA GCCCAACGGT TACCTGCACA TCGGCCACGC CAAGTCGATC TGCCTGAACT TCGGCCTGGC CCAGGAATTC GCTGGCGATT GCCATCTGCG CTTCGATGAC ACCAACCCGG CCAAGGAAGA CCAGGAGTAC ATCGATGCCA TCGAGGCCGA CATCAAGTGG CTGGGCTTCC AGTGGTCCGG CGAGGTCTGC TATGCCTCCA ACTATTTCGA CCAGCTGCAT GCCTGGGCCG TCGAGCTGAT CAAGGCCGGC AAGGCGTTCG TCTGCGACCT CGGTCCCGAG GAAATGCGCG AATACCGCGG CACCCTCACC GAGCCGGGCC GCAACAGCCC TTACCGTGAC CGTTCGGTGG AGGAGAACCT CGACCTGTTC GCCCGCATGA AGGCCGGTGA GTTCCCCGAC GGCGCCCGTT CGCTGCGGGC GAAGATCGAC ATGGGTTCGC CGAACATGAA CCTGCGCGAT CCGATCCTGT ACCGCATCCG CCATGCGCAT CACCACCAGA CCGGCGACAA GTGGTGCATC TACCCGAGCT ACGACTTCAC CCACGGTCAG TCGGACGCCA TCGAGGGCAT CACCCATTCG ATCTGCACCC TGGAATTCGA AGACCATCGT CCGCTCTACG AGTGGTTCCT GGCCAACCTG CCGGTACCTG CGCAACCGCG CCAGTACGAA TTCTCCCGGC TCAACCTGAA CTACACCGTG ACCAGCAAGC GCAAGCTCAA GCAATTGGTC GACGAAGGCC ATGTCAGCGG CTGGGACGAT CCGCGCATGT CGACCCTGTC CGGCTATCGC CGCCGCGGCT ACACCCCGGA GTCGATCCGC AACTTCTGCG AGATGATCGG GGTGAACCGC GCCAGCGGCG TGGTCGACAT CGGCATGCTC GAGTTCAGCA TCCGCGACCA CCTGGACGCC ACCGCGCCGC GTGCCATGTG CGTGCTGAAA CCGCTCAAGG TGGTGATCAC CAACTACCCC GAAGGGCAGG TGGAGAACCT CGAGTTGCCG CGTCATCCGA AGGAAGACAT GGGAGTGCGC GTACTGCCGT TCGGTCGCGA GCTGTTCATC GATGCCGGTG ACTTCGAGGA AGTCCCGCCG GCCGGCTACA AGCGCCTGAT CCCCGGTGGC GAGGTGCGTC TGCGCGGCAG CTACGTGATC CGTGCCGACG AAGCGATCAA GGATGCCGAC GGCAATATCG TCGAACTGCG CTGCAGCTAC GATCCGGACA CCCTGGGCAA GAACCCGGAA GGCCGCAAGG TCAAGGGCGT GATCCACTGG GTGCCGGCCG AGGGCAGCGT CGAGTGCGAA GTGCGCCTGT ACGATCGGCT GTTCCGCTCG GCCAATCCGG AGAAGGCGGA AGAGGGCGGC AGCTTCCTCG ACAACATCAA CGCCGACTCC CTGCAGGTAC TGGCCGGCTG TCGCGCCGAG CCGTCGCTGG GCCAGGCGAA CCCGGAGGAT CGCTTCCAGT TCGAGCGCGA AGGCTATTTC GTCGCCGACC TGAAGGACTC CAGGCCGGGC AAGCCGGTGT TCAACCGTAC CGTCACCCTG CGTGACTCCT GGGGACAGGG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT