PsaeA.18138.a

Uncharacterized protein (HHPRED: putative cyclic diguanylate phosphodiesterase)

CENTER ID: PsaeA.18138.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18138.a.B1.GE39448 full length 1 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.2229
PGAT: pseudomonas:PA2133
RefSeq: NP_250823.1
UniProt: Q9I1Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGSPQARQH EHPPAVAASL AGDLRLARRF QAALARGRVR LDWQAVRHAG HPEWEPLYRE TLLRVTAASG EPPLPTQELI LALERLGLVR LLDRCVLGTV LDRLNAEPTL RLACNLSRQS AAMDAWWEAV CRWLAARPQV ARRLTLELTE TAVGERVATR EFIRRLREHG VRIAIDDFGA AHNNLDFVLD ARPDVIKIDC RYTREARRSA KGAEVLRHLL ALCRELAPCV VLEGLEEDDA FARLPTGDVY LQGNAIAPPM RVEPPLSVRR PKSRARIEAL ASRQG
NT Sequence
gtgaacggtt ccccacaggc tcgccagcac gagcacccac cggccgttgc cgcctccctg gccggcgacc tgcgcctcgc ccggcgtttc caggccgccc tggcgcgcgg tcgggtgcgc ctggactggc aggccgtacg gcatgccggg catccggaat gggagccgct ctaccgggaa accctgctca gggttacggc cgccagcggc gagccgccgt tgccgaccca ggaactgatc ctggcgctgg agcggctcgg cctggtacgc ctgctcgatc gctgcgtgct gggcacggtc ctcgaccggc tgaacgccga gcctaccttg cgcctggcct gcaacctgtc gcgccagagc gcggcgatgg atgcctggtg ggaagcggtc tgccgatggc tggccgcccg tccgcaggtg gcacggaggc tgacgctgga gctgaccgag accgccgtgg gcgagagagt cgcgacccgg gagttcatcc gacgcctgcg cgaacatggc gtgcgcatcg cgatagacga cttcggcgcg gcgcacaaca acctggactt cgttctcgat gcccgtcccg atgtgatcaa gatcgattgc cggtataccc gcgaagcacg tcgctcggcg aagggcgccg aagtgctgcg ccatctgctg gccctgtgcc gcgaactggc gccctgcgtg gtcctggaag gtctcgagga ggacgatgcg ttcgcccggc tgccgaccgg ggacgtctat ctccagggca acgcgatcgc gccgccgatg cgggtcgaac cgccgctgtc cgtacgccgg ccgaagagcc gggcgcggat cgaggcgctg gcgagccgcc aggggtga
Details for PsaeA.18138.a.B1.GE39448
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV NGSPQARQHE HPPAVAASLA GDLRLARRFQ AALARGRVRL DWQAVRHAGH PEWEPLYRET LLRVTAASGE PPLPTQELIL ALERLGLVRL LDRCVLGTVL DRLNAEPTLR LACNLSRQSA AMDAWWEAVC RWLAARPQVA RRLTLELTET AVGERVATRE FIRRLREHGV RIAIDDFGAA HNNLDFVLDA RPDVIKIDCR YTREARRSAK GAEVLRHLLA LCRELAPCVV LEGLEEDDAF ARLPTGDVYL QGNAIAPPMR VEPPLSVRRP KSRARXXXXA SRQG
Validated NT Sequence
ttacccctgg cggctcgccn nnnnnnnnnn ncgcgcccgg ctcttcggcc ggcgtacgga cagcggcggt tcgacccgca tcggcggcgc gatcgcgttg ccctggagat agacgtcccc ggtcggcagc cgggcgaacg catcgtcctc ctcgagacct tccaggacca cgcagggcgc cagttcgcgg cacagggcca gcagatggcg cagcacttcg gcgcccttcg ccgagcgacg tgcttcgcgg gtataccggc aatcgatctt gatcacatcg ggacgggcat cgagaacgaa gtccaggttg ttgtgcgccg cgccgaagtc gtctatcgcg atgcgcacgc catgttcgcg caggcgtcgg atgaactccc gggtcgcgac tctctcgccc acggcggtct cggtcagctc cagcgtcagc ctccgtgcca cctgcggacg ggcggccagc catcggcaga ccgcttccca ccaggcatcc atcgccgcgc tctggcgcga caggttgcag gccaggcgca aggtaggctc ggcgttcagc cggtcgagga ccgtgcccag cacgcagcga tcgagcaggc gtaccaggcc gagccgctcc agcgccagga tcagttcctg ggtcggcaac ggcggctcgc cgctggcggc cgtaaccctg agcagggttt cccggtagag cggctcccat tccggatgcc cggcatgccg tacggcctgc cagtccaggc gcacccgacc gcgcgccagg gcggcctgga aacgccgggc gaggcgcagg tcgccggcca gggaggcggc aacggccggt gggtgctcgt gctggcgagc ctgtggggaa ccgttcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN GSPQARQHEH PPAVAASLAG DLRLARRFQA ALARGRVRLD WQAVRHAGHP EWEPLYRETL LRVTAASGEP PLPTQELILA LERLGLVRLL DRCVLGTVLD RLNAEPTLRL ACNLSRQSAA MDAWWEAVCR WLAARPQVAR RLTLELTETA VGERVATREF IRRLREHGVR IAIDDFGAAH NNLDFVLDAR PDVIKIDCRY TREARRSAKG AEVLRHLLAL CRELAPCVVL EGLEEDDAFA RLPTGDVYLQ GNAIAPPMRV EPPLSVRRPK SRARIEALAS RQG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaacg gttccccaca ggctcgccag cacgagcacc caccggccgt tgccgcctcc ctggccggcg acctgcgcct cgcccggcgt ttccaggccg ccctggcgcg cggtcgggtg cgcctggact ggcaggccgt acggcatgcc gggcatccgg aatgggagcc gctctaccgg gaaaccctgc tcagggttac ggccgccagc ggcgagccgc cgttgccgac ccaggaactg atcctggcgc tggagcggct cggcctggta cgcctgctcg atcgctgcgt gctgggcacg gtcctcgacc ggctgaacgc cgagcctacc ttgcgcctgg cctgcaacct gtcgcgccag agcgcggcga tggatgcctg gtgggaagcg gtctgccgat ggctggccgc ccgtccgcag gtggcacgga ggctgacgct ggagctgacc gagaccgccg tgggcgagag agtcgcgacc cgggagttca tccgacgcct gcgcgaacat ggcgtgcgca tcgcgataga cgacttcggc gcggcgcaca acaacctgga cttcgttctc gatgcccgtc ccgatgtgat caagatcgat tgccggtata cccgcgaagc acgtcgctcg gcgaagggcg ccgaagtgct gcgccatctg ctggccctgt gccgcgaact ggcgccctgc gtggtcctgg aaggtctcga ggaggacgat gcgttcgccc ggctgccgac cggggacgtc tatctccagg gcaacgcgat cgcgccgccg atgcgggtcg aaccgccgct gtccgtacgc cggccgaaga gccgggcgcg gatcgaggcg ctggcgagcc gccaggggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat