PsaeA.18062.a

DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I) (Omega-protein) (Relaxing enzyme) (Swivelase) (Untwisting enzyme)

CENTER ID: PsaeA.18062.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.18062.a.B1.GE38527 full length 1 868
PsaeA.18062.a.B2.GE38528 structural domain (4CGY_A) 1 743
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.18062.a.B2.PS02342 structural domain (4CGY_A) 1 743

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.3159
PGAT: pseudomonas:PA3011
RefSeq: NP_251701.1
UniProt: Q9HZJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKSLVIVES PAKAKTINKY LGSQYVVKSS IGHIRDLPTS GSSSSKEPAA KGRKSASEAP ALSPKEKARR QLVSRMGVDP EHGWKAKYEI LPGKEKVIDE LRRLAKDADT VYLATDLDRE GEAIAWHLRE AIGGDESRYK RVVFNEITKK AIQEAFSQPG ELDINRVNAQ QARRFLDRVV GYMVSPLLWQ KIARGLSAGR VQSVAVKLVV EREREIRAFV PEEYWEVHAD LGTAKGANVR FEVTREKGEA FKPLNEAQAM AALEKLKASA YSVAKREDRP TSSRPSAPFI TSTLQQAASN RLGFGVKKTM MMAQRLYEAG YITYMRTDST NLSADAIGMV RGFIEDEFGQ KYLPGKANVY SSKEGAQEAH EAIRPSDVNL KPTQLSGMER DAERLYDLIW RQFVACQMTP AEYLSTSVSV TAGDFELRAK GRILKFDGYT RVLPQQSKPG EDDVLPEMKE GENLKLIKLD PSQHFTKPPA RYSEASLVKE LEKRGIGRPS TYAAIISTIQ ERGYVTTHNR RFYAEKMGDI VTDRLNESFA NLMDYGFTAG MEEHLDDVAQ GERDWKHLLD EFYGDFKKKL EVAEVSEKGM RANQPTLTNI PCRECGRPMM IRTASTGVFL GCSGYSLPPK ERCKATVNLI PGDEIAADDE GESESRVLRG KHRCPICSTA MDAYLLDEKH KLHICGNNPD CPGYEIEEGQ YRIKGYEGPS LECDKCGSEM QLKTGRFGKF FGCTNPTCKN TRKLLKNGEA APPKMDAIRM PELKCEKVDD IYVLRDGASG MFLAASQFPK NRETRAPLVS EIIPHKAELD PKYHYLCDAP QKDPDGRPAV IRFSRKTKEQ YVQSEVDGKP TGWRAFYDGG KWKVEDKR
NT Sequence
atgggtaaat cgctggtcat cgtggaatcc ccggccaagg ccaagaccat caacaagtac ctgggcagcc agtacgtggt gaagtcgagc atcggccaca tccgtgacct gcccaccagt ggttcgtcga gttccaagga gccggcggcc aagggccgca agagcgcctc cgaggcgccg gcgctgtcac cgaaggagaa ggccaggcgc caactggtct cgcgcatggg ggtcgacccc gagcatggct ggaaggccaa gtacgagatc ctgcccggca aggaaaaggt catcgacgag ctgcgccgcc tggccaagga cgccgacacc gtctatctcg cgaccgactt ggaccgcgaa ggggaggcca tcgcctggca cctgcgggaa gccatcggcg gcgacgaaag ccgctacaag cgcgtggtat tcaacgaaat caccaagaag gccatccagg aggccttctc ccagcccggc gagctcgata tcaaccgggt gaacgcccag caggcgcggc gtttcctcga ccgcgtggtc ggctacatgg tctcgccgct gctctggcag aagattgccc gtgggttgtc cgccggccgc gtgcagtcgg tggcggtgaa gctggtggtg gagcgcgaac gcgagattcg cgccttcgtc ccggaagagt actgggaagt gcatgccgac ctcggtaccg ccaagggcgc caacgtgcgc ttcgaggtta cccgtgagaa gggcgaggcc ttcaagccgc tcaacgaagc ccaggccatg gcggccctgg aaaagctcaa ggcctcggcc tatagcgtcg ccaagcgcga ggaccggccg acctccagcc gaccctcggc gccgttcatc acttccaccc tgcagcaggc ggcgagcaac cgcctgggct tcggggtgaa gaagaccatg atgatggccc agcgtctcta cgaggccggc tacatcacct acatgcgtac cgactcgacc aacctgtcgg ccgatgccat tggcatggtc cgcggtttca tcgaggacga gttcggccag aagtacctgc cgggcaaggc caacgtctac tcgagcaagg agggcgccca ggaagcgcac gaggcgatcc gtccttccga cgtcaacctc aagccgaccc agctgtccgg catggagcgt gacgccgagc gcctgtacga cctgatctgg cgccagttcg tggcgtgcca gatgaccccg gccgaatacc tctcgaccag cgtgagcgtc accgccggcg atttcgagct gcgcgccaag ggccgcatcc tcaagttcga cggttatacc cgggtactgc cgcagcagag caagccgggc gaggacgacg tcctgccgga aatgaaggaa ggcgagaacc tcaagctgat caagctcgat ccgagccagc acttcaccaa gccgccggcg cgctactccg aagcgagcct ggtcaaggaa ctggagaagc gcggtatcgg ccgcccctcg acctacgcgg cgatcatctc caccatccag gagcgcggct acgtaaccac ccacaaccgc cgcttctatg cggagaagat gggcgacatc gtcaccgatc ggctcaacga gagcttcgcc aacctgatgg actacggttt caccgccggg atggaggagc atctcgacga cgtggcccag ggcgagcgcg actggaagca cctgctcgac gagttctacg gcgatttcaa gaagaagctc gaggtggccg aagtcagcga gaaggggatg cgcgccaacc agccgaccct gaccaacatt ccctgccgcg agtgcggccg gccgatgatg attcgtaccg cctccaccgg cgtgttcctc ggctgctcgg gctacagcct gccgccgaag gagcgttgca aggcgaccgt caacctgatt cccggcgacg agatcgccgc ggacgacgag ggcgagtccg agtcgcgggt gttgcgtggc aagcatcgct gcccgatctg cagtaccgcg atggacgcct acctgctgga cgagaaacac aagctgcata tctgcggcaa caaccccgac tgtccgggct acgagatcga ggaaggccag taccggatca agggctacga agggccgagc ctggaatgcg acaagtgtgg cagcgagatg cagttgaaga ccggccgttt cggcaagttc ttcggttgca ccaatccgac ctgcaagaac acccgcaagc tgctgaagaa tggcgaggcg gcgccgccga agatggacgc catccgcatg cctgaattga agtgcgagaa ggtggacgat atctacgtgc tgcgcgacgg cgcttcgggc atgttcctgg ccgccagcca gttcccgaag aaccgcgaga cccgcgcgcc gttggtgagc gagatcatcc cgcacaaggc cgagctggat cccaagtacc attacctctg cgacgccccg cagaaagacc cggacggccg tcccgcggtg atccgtttca gccgcaagac caaggagcaa tacgtgcagt ccgaggtcga tggcaagccc accggctggc gcgccttcta cgatggcggc aagtggaagg tcgaagacaa gcgctga
Details for PsaeA.18062.a.B1.GE38527
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 98kDa
OBSERVED MW: 98kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG KSLVIVESPA KAKTINKYLG SQYVVKSSIG HIRDLPTSGS SSSKEPAAKG RKSASEAPAL SPKEKARRQL VSRMGVDPEH GWKAKYEILP GKEKVIDELR RLAKDADTVY LATDLDREGE AIAWHLREAI GGDESRYKRV VFNEITKKAI QEAFSQPGEL DINRVNAQQA RRFLDRVVGY MVSPLLWQKI ARGLSAGRVQ SVAVKLVVER EREIRAFVPE EYWEVHADLG TAKGANVRFE VTREKGEAFK PLNEAQAMAA LEKLKASAYS VAKREDRPTS SRPSAPFITS TLQQAASNRL GFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SADAIGMVRG FIEDEFGQKY LPGKANVYSS KEGAQEAHEA IRPSDVNLKP TQLSGMERDA ERLYDLIWRQ FVACQMTPAE YLSTSVSVTA GDFELRAKGR ILKFDGYTRV LPQQSKPGED DVLPEMKEGE NLKLIKLDPS QHFTKPPARY SEASLVKELE KRGIGRPSTY AAIISTIQER GYVTTHNRRF YAEKMGDIVT DRLNESFANL MDYGFTAGME EHLDDVAQGE RDWKHLLDEF YGDFKKKLEV AEVSEKGMRA NQPTLTNIPC RECGRPMMIR TASTGVFLGC SGYSLPPKER CKATVNLIPG DEIAADDEGE SESRVLRGKH RCPICSTAMD AYLLDEKHKL HICGNNPDCP GYEIEEGQYR IKGYEGPSLE CDKCGSEMQL KTGRFGKFFG CTNPTCKNTR KLLKNGEAAP PKMDAIRMPE LKCEKVDDIY VLRDGASGMF LAASQFPKNR ETRAPLVSEI IPHKAELDPK YHYLCDAPQK DPDGRPAVIR FSRKTKEQYV QSEVDGKPTG WRAFYDGGKW KVEDKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta aatcgctggt catcgtggaa tccccggcca aggccaagac catcaacaag tacctgggca gccagtacgt ggtgaagtcg agcatcggcc acatccgtga cctgcccacc agtggttcgt cgagttccaa ggagccggcg gccaagggcc gcaagagcgc ctccgaggcg ccggcgctgt caccgaagga gaaggccagg cgccaactgg tctcgcgcat gggggtcgac cccgagcatg gctggaaggc caagtacgag atcctgcccg gcaaggaaaa ggtcatcgac gagctgcgcc gcctggccaa ggacgccgac accgtctatc tcgcgaccga cttggaccgc gaaggggagg ccatcgcctg gcacctgcgg gaagccatcg gcggcgacga aagccgctac aagcgcgtgg tattcaacga aatcaccaag aaggccatcc aggaggcctt ctcccagccc ggcgagctcg atatcaaccg ggtgaacgcc cagcaggcgc ggcgtttcct cgaccgcgtg gtcggctaca tggtctcgcc gctgctctgg cagaagattg cccgtgggtt gtccgccggc cgcgtgcagt cggtggcggt gaagctggtg gtggagcgcg aacgcgagat tcgcgccttc gtcccggaag agtactggga agtgcatgcc gacctcggta ccgccaaggg cgccaacgtg cgcttcgagg ttacccgtga gaagggcgag gccttcaagc cgctcaacga agcccaggcc atggcggccc tggaaaagct caaggcctcg gcctatagcg tcgccaagcg cgaggaccgg ccgacctcca gccgaccctc ggcgccgttc atcacttcca ccctgcagca ggcggcgagc aaccgcctgg gcttcggggt gaagaagacc atgatgatgg cccagcgtct ctacgaggcc ggctacatca cctacatgcg taccgactcg accaacctgt cggccgatgc cattggcatg gtccgcggtt tcatcgagga cgagttcggc cagaagtacc tgccgggcaa ggccaacgtc tactcgagca aggagggcgc ccaggaagcg cacgaggcga tccgtccttc cgacgtcaac ctcaagccga cccagctgtc cggcatggag cgtgacgccg agcgcctgta cgacctgatc tggcgccagt tcgtggcgtg ccagatgacc ccggccgaat acctctcgac cagcgtgagc gtcaccgccg gcgatttcga gctgcgcgcc aagggccgca tcctcaagtt cgacggttat acccgggtac tgccgcagca gagcaagccg ggcgaggacg acgtcctgcc ggaaatgaag gaaggcgaga acctcaagct gatcaagctc gatccgagcc agcacttcac caagccgccg gcgcgctact ccgaagcgag cctggtcaag gaactggaga agcgcggtat cggccgcccc tcgacctacg cggcgatcat ctccaccatc caggagcgcg gctacgtaac cacccacaac cgccgcttct atgcggagaa gatgggcgac atcgtcaccg atcggctcaa cgagagcttc gccaacctga tggactacgg tttcaccgcc gggatggagg agcatctcga cgacgtggcc cagggcgagc gcgactggaa gcacctgctc gacgagttct acggcgattt caagaagaag ctcgaggtgg ccgaagtcag cgagaagggg atgcgcgcca accagccgac cctgaccaac attccctgcc gcgagtgcgg ccggccgatg atgattcgta ccgcctccac cggcgtgttc ctcggctgct cgggctacag cctgccgccg aaggagcgtt gcaaggcgac cgtcaacctg attcccggcg acgagatcgc cgcggacgac gagggcgagt ccgagtcgcg ggtgttgcgt ggcaagcatc gctgcccgat ctgcagtacc gcgatggacg cctacctgct ggacgagaaa cacaagctgc atatctgcgg caacaacccc gactgtccgg gctacgagat cgaggaaggc cagtaccgga tcaagggcta cgaagggccg agcctggaat gcgacaagtg tggcagcgag atgcagttga agaccggccg tttcggcaag ttcttcggtt gcaccaatcc gacctgcaag aacacccgca agctgctgaa gaatggcgag gcggcgccgc cgaagatgga cgccatccgc atgcctgaat tgaagtgcga gaaggtggac gatatctacg tgctgcgcga cggcgcttcg ggcatgttcc tggccgccag ccagttcccg aagaaccgcg agacccgcgc gccgttggtg agcgagatca tcccgcacaa ggccgagctg gatcccaagt accattacct ctgcgacgcc ccgcagaaag acccggacgg ccgtcccgcg gtgatccgtt tcagccgcaa gaccaaggag caatacgtgc agtccgaggt cgatggcaag cccaccggct ggcgcgcctt ctacgatggc ggcaagtgga aggtcgaaga caagcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.18062.a.B2.GE38528
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 84kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG KSLVIVESPA KAKTINKYLG SQYVVKSSIG HIRDLPTSGS SSSKEPAAKG RKSASEAPAL SPKEKARRQL VSRMGVDPEH GWKAKYEILP GKEKVIDELR RLAKDADTVY LATDLDREGE AIAWHLREAI GGDESRYKRV VFNEITKKAI QEAFSQPGEL DINRVNAQQA RRFLDRVVGY MVSPLLWQKI ARGLSAGRVQ SVAVKLVVER EREIRAFVPE EYWEVHADLG TAKGANVRFE VTREKGEAFK PLNEAQAMAA LEKLKASAYS VAKREDRPTS SRPSAPFITS TLQQAASNRL GFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SADAIGMVRG FIEDEFGQKY LPGKANVYSS KEGAQEAHEA IRPSDVNLKP TQLSGMERDA ERLYDLIWRQ FVACQMTPAE YLSTSVSVTA GDFELRAKGR ILKFDGYTRV LPQQSKPGED DVLPEMKEGE NLKLIKLDPS QHFTKPPARY SEASLVKELE KRGIGRPSTY AAIISTIQER GYVTTHNRRF YAEKMGDIVT DRLNESFANL MDYGFTAGME EHLDDVAQGE RDWKHLLDEF YGDFKKKLEV AEVSEKGMRA NQPTLTNIPC RECGRPMMIR TASTGVFLGC SGYSLPPKER CKATVNLIPG DEIAADDEGE SESRVLRGKH RCPICSTAMD AYLLDEKHKL HICGNNPDCP GYEIEEGQYR IKGYEGPSLE CDKCGSEMQL KTGRFGKFFG CTNPTCKNTR K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta aatcgctggt catcgtggaa tccccggcca aggccaagac catcaacaag tacctgggca gccagtacgt ggtgaagtcg agcatcggcc acatccgtga cctgcccacc agtggttcgt cgagttccaa ggagccggcg gccaagggcc gcaagagcgc ctccgaggcg ccggcgctgt caccgaagga gaaggccagg cgccaactgg tctcgcgcat gggggtcgac cccgagcatg gctggaaggc caagtacgag atcctgcccg gcaaggaaaa ggtcatcgac gagctgcgcc gcctggccaa ggacgccgac accgtctatc tcgcgaccga cttggaccgc gaaggggagg ccatcgcctg gcacctgcgg gaagccatcg gcggcgacga aagccgctac aagcgcgtgg tattcaacga aatcaccaag aaggccatcc aggaggcctt ctcccagccc ggcgagctcg atatcaaccg ggtgaacgcc cagcaggcgc ggcgtttcct cgaccgcgtg gtcggctaca tggtctcgcc gctgctctgg cagaagattg cccgtgggtt gtccgccggc cgcgtgcagt cggtggcggt gaagctggtg gtggagcgcg aacgcgagat tcgcgccttc gtcccggaag agtactggga agtgcatgcc gacctcggta ccgccaaggg cgccaacgtg cgcttcgagg ttacccgtga gaagggcgag gccttcaagc cgctcaacga agcccaggcc atggcggccc tggaaaagct caaggcctcg gcctatagcg tcgccaagcg cgaggaccgg ccgacctcca gccgaccctc ggcgccgttc atcacttcca ccctgcagca ggcggcgagc aaccgcctgg gcttcggggt gaagaagacc atgatgatgg cccagcgtct ctacgaggcc ggctacatca cctacatgcg taccgactcg accaacctgt cggccgatgc cattggcatg gtccgcggtt tcatcgagga cgagttcggc cagaagtacc tgccgggcaa ggccaacgtc tactcgagca aggagggcgc ccaggaagcg cacgaggcga tccgtccttc cgacgtcaac ctcaagccga cccagctgtc cggcatggag cgtgacgccg agcgcctgta cgacctgatc tggcgccagt tcgtggcgtg ccagatgacc ccggccgaat acctctcgac cagcgtgagc gtcaccgccg gcgatttcga gctgcgcgcc aagggccgca tcctcaagtt cgacggttat acccgggtac tgccgcagca gagcaagccg ggcgaggacg acgtcctgcc ggaaatgaag gaaggcgaga acctcaagct gatcaagctc gatccgagcc agcacttcac caagccgccg gcgcgctact ccgaagcgag cctggtcaag gaactggaga agcgcggtat cggccgcccc tcgacctacg cggcgatcat ctccaccatc caggagcgcg gctacgtaac cacccacaac cgccgcttct atgcggagaa gatgggcgac atcgtcaccg atcggctcaa cgagagcttc gccaacctga tggactacgg tttcaccgcc gggatggagg agcatctcga cgacgtggcc cagggcgagc gcgactggaa gcacctgctc gacgagttct acggcgattt caagaagaag ctcgaggtgg ccgaagtcag cgagaagggg atgcgcgcca accagccgac cctgaccaac attccctgcc gcgagtgcgg ccggccgatg atgattcgta ccgcctccac cggcgtgttc ctcggctgct cgggctacag cctgccgccg aaggagcgtt gcaaggcgac cgtcaacctg attcccggcg acgagatcgc cgcggacgac gagggcgagt ccgagtcgcg ggtgttgcgt ggcaagcatc gctgcccgat ctgcagtacc gcgatggacg cctacctgct ggacgagaaa cacaagctgc atatctgcgg caacaacccc gactgtccgg gctacgagat cgaggaaggc cagtaccgga tcaagggcta cgaagggccg agcctggaat gcgacaagtg tggcagcgag atgcagttga agaccggccg tttcggcaag ttcttcggtt gcaccaatcc gacctgcaag aacacccgca agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PsaeA.18062.a.B2.PS02342
PURIFICATION DATe: 2/26/2015
CONCENTRATION: 33.52mg/ml
OBSERVED MW: 82kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG KSLVIVESPA KAKTINKYLG SQYVVKSSIG HIRDLPTSGS SSSKEPAAKG RKSASEAPAL SPKEKARRQL VSRMGVDPEH GWKAKYEILP GKEKVIDELR RLAKDADTVY LATDLDREGE AIAWHLREAI GGDESRYKRV VFNEITKKAI QEAFSQPGEL DINRVNAQQA RRFLDRVVGY MVSPLLWQKI ARGLSAGRVQ SVAVKLVVER EREIRAFVPE EYWEVHADLG TAKGANVRFE VTREKGEAFK PLNEAQAMAA LEKLKASAYS VAKREDRPTS SRPSAPFITS TLQQAASNRL GFGVKKTMMM AQRLYEAGYI TYMRTDSTNL SADAIGMVRG FIEDEFGQKY LPGKANVYSS KEGAQEAHEA IRPSDVNLKP TQLSGMERDA ERLYDLIWRQ FVACQMTPAE YLSTSVSVTA GDFELRAKGR ILKFDGYTRV LPQQSKPGED DVLPEMKEGE NLKLIKLDPS QHFTKPPARY SEASLVKELE KRGIGRPSTY AAIISTIQER GYVTTHNRRF YAEKMGDIVT DRLNESFANL MDYGFTAGME EHLDDVAQGE RDWKHLLDEF YGDFKKKLEV AEVSEKGMRA NQPTLTNIPC RECGRPMMIR TASTGVFLGC SGYSLPPKER CKATVNLIPG DEIAADDEGE SESRVLRGKH RCPICSTAMD AYLLDEKHKL HICGNNPDCP GYEIEEGQYR IKGYEGPSLE CDKCGSEMQL KTGRFGKFFG CTNPTCKNTR K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTA AATCGCTGGT CATCGTGGAA TCCCCGGCCA AGGCCAAGAC CATCAACAAG TACCTGGGCA GCCAGTACGT GGTGAAGTCG AGCATCGGCC ACATCCGTGA CCTGCCCACC AGTGGTTCGT CGAGTTCCAA GGAGCCGGCG GCCAAGGGCC GCAAGAGCGC CTCCGAGGCG CCGGCGCTGT CACCGAAGGA GAAGGCCAGG CGCCAACTGG TCTCGCGCAT GGGGGTCGAC CCCGAGCATG GCTGGAAGGC CAAGTACGAG ATCCTGCCCG GCAAGGAAAA GGTCATCGAC GAGCTGCGCC GCCTGGCCAA GGACGCCGAC ACCGTCTATC TCGCGACCGA CTTGGACCGC GAAGGGGAGG CCATCGCCTG GCACCTGCGG GAAGCCATCG GCGGCGACGA AAGCCGCTAC AAGCGCGTGG TATTCAACGA AATCACCAAG AAGGCCATCC AGGAGGCCTT CTCCCAGCCC GGCGAGCTCG ATATCAACCG GGTGAACGCC CAGCAGGCGC GGCGTTTCCT CGACCGCGTG GTCGGCTACA TGGTCTCGCC GCTGCTCTGG CAGAAGATTG CCCGTGGGTT GTCCGCCGGC CGCGTGCAGT CGGTGGCGGT GAAGCTGGTG GTGGAGCGCG AACGCGAGAT TCGCGCCTTC GTCCCGGAAG AGTACTGGGA AGTGCATGCC GACCTCGGTA CCGCCAAGGG CGCCAACGTG CGCTTCGAGG TTACCCGTGA GAAGGGCGAG GCCTTCAAGC CGCTCAACGA AGCCCAGGCC ATGGCGGCCC TGGAAAAGCT CAAGGCCTCG GCCTATAGCG TCGCCAAGCG CGAGGACCGG CCGACCTCCA GCCGACCCTC GGCGCCGTTC ATCACTTCCA CCCTGCAGCA GGCGGCGAGC AACCGCCTGG GCTTCGGGGT GAAGAAGACC ATGATGATGG CCCAGCGTCT CTACGAGGCC GGCTACATCA CCTACATGCG TACCGACTCG ACCAACCTGT CGGCCGATGC CATTGGCATG GTCCGCGGTT TCATCGAGGA CGAGTTCGGC CAGAAGTACC TGCCGGGCAA GGCCAACGTC TACTCGAGCA AGGAGGGCGC CCAGGAAGCG CACGAGGCGA TCCGTCCTTC CGACGTCAAC CTCAAGCCGA CCCAGCTGTC CGGCATGGAG CGTGACGCCG AGCGCCTGTA CGACCTGATC TGGCGCCAGT TCGTGGCGTG CCAGATGACC CCGGCCGAAT ACCTCTCGAC CAGCGTGAGC GTCACCGCCG GCGATTTCGA GCTGCGCGCC AAGGGCCGCA TCCTCAAGTT CGACGGTTAT ACCCGGGTAC TGCCGCAGCA GAGCAAGCCG GGCGAGGACG ACGTCCTGCC GGAAATGAAG GAAGGCGAGA ACCTCAAGCT GATCAAGCTC GATCCGAGCC AGCACTTCAC CAAGCCGCCG GCGCGCTACT CCGAAGCGAG CCTGGTCAAG GAACTGGAGA AGCGCGGTAT CGGCCGCCCC TCGACCTACG CGGCGATCAT CTCCACCATC CAGGAGCGCG GCTACGTAAC CACCCACAAC CGCCGCTTCT ATGCGGAGAA GATGGGCGAC ATCGTCACCG ATCGGCTCAA CGAGAGCTTC GCCAACCTGA TGGACTACGG TTTCACCGCC GGGATGGAGG AGCATCTCGA CGACGTGGCC CAGGGCGAGC GCGACTGGAA GCACCTGCTC GACGAGTTCT ACGGCGATTT CAAGAAGAAG CTCGAGGTGG CCGAAGTCAG CGAGAAGGGG ATGCGCGCCA ACCAGCCGAC CCTGACCAAC ATTCCCTGCC GCGAGTGCGG CCGGCCGATG ATGATTCGTA CCGCCTCCAC CGGCGTGTTC CTCGGCTGCT CGGGCTACAG CCTGCCGCCG AAGGAGCGTT GCAAGGCGAC CGTCAACCTG ATTCCCGGCG ACGAGATCGC CGCGGACGAC GAGGGCGAGT CCGAGTCGCG GGTGTTGCGT GGCAAGCATC GCTGCCCGAT CTGCAGTACC GCGATGGACG CCTACCTGCT GGACGAGAAA CACAAGCTGC ATATCTGCGG CAACAACCCC GACTGTCCGG GCTACGAGAT CGAGGAAGGC CAGTACCGGA TCAAGGGCTA CGAAGGGCCG AGCCTGGAAT GCGACAAGTG TGGCAGCGAG ATGCAGTTGA AGACCGGCCG TTTCGGCAAG TTCTTCGGTT GCACCAATCC GACCTGCAAG AACACCCGCA AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT