PsaeA.18055.a

DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)

CENTER ID: PsaeA.18055.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18055.a.B1.GE38523 full length 1 1357
PsaeA.18055.a.B2.GE38524 structural domain (4KBM_A) 14 519
PsaeA.18055.a.B3.GE38525 structural domain (3TBI_B) 830 1081
PsaeA.18055.a.B4.GE38526 RNA polymerase beta subunit, domains 6-7 720 1356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.18055.a.B2.PS02307 structural domain (4KBM_A) 14 519

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4471
PGAT: pseudomonas:PA4270
RefSeq: NP_252960.1
UniProt: Q51561

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGATKEQFRD VGLHAAFKSV FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD RESSNKAIKD IKEQEVYMGE IPLMTENGTF IINGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI IPYRGSWLDF EFDPKDCVFV RIDRRRKLPA SVLLRALGYS TEEILNAFYA TNVFHIKGET LNLELVPQRL RGEVASIDIK DGSGKVIVEQ GRRITARHIN QLEKAGVSQL EVPFDYLIGR TIAKAIVHPA TGEIIAECNT ELTLDLLAKV AKAQVVRIET LYTNDIDCGP FISDTLKIDN TSNQLEALVE IYRMMRPGEP PTKEAAETLF GNLFFSAERY DLSAVGRMKF NRRIGRTEIE GPGVLSKEDI IDVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV KERLSMAESE GLMPQDLINA KPVAAAIKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL ATYARTNKYG FLESPYRVVK DSLVTDEIVF LSAIEEADHV IAQASATLNE KGQLVDELVA VRHLNEFTVK APEDVTLMDV SPKQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC VVARRGGVID SVDASRVVVR VADDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD VVARGDILAD GPSTDMGELA LGQNMRVAFM PWNGFNFEDS ICLSERVVQE DRFTTIHIQE LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVQAGDILVG KVTPKGETQL TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDSRA LSIEKMQLDQ IRKDLNEEFR IVEGATFERL RAALVGAKAE GGPALKKGTE ITDDYLDGLE RGQWFKLRMA DDALNEQLEK AQAYISDRRQ LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIKRRIQPG DKMAGRHGNK GVVSVIMPVE DMPHDANGTP VDIVLNPLGV PSRMNVGQIL ETHLGLAAKG LGEKINRMLE EQRKVAELRK FLHEIYNEIG GREENLDELG DNEILALAKN LRGGVPMATP VFDGAKEREI KAMLKLADLP ESGQMRLFDG RTGNQFERPT TVGYMYMLKL NHLVDDKMHA RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY TLQEMLTVKS DDVNGRTKMY KNIVDGDHRM EAGMPESFNV LIKEIRSLGI DIELETE
NT Sequence
ATGGCTTACT CATACACTGA GAAAAAACGT ATCCGCAAGG ACTTTAGCAA GTTGCCGGAC GTCATGGATG TGCCGTATTT GCTGGCCATC CAGCTGGATT CCTATCGCGA ATTCCTGCAG GCTGGCGCAA CCAAGGAGCA GTTCCGCGAT GTCGGTCTGC ACGCGGCCTT CAAGTCCGTT TTCCCGATTA TCAGCTATTC CGGCAATGCT GCCCTGGAAT ACGTCGGCTA CCGTCTGGGT GAGCCGGCAT TCGATGTCAA GGAGTGCGTG CTGCGCGGCG TGACCTTCGC CGTACCGCTG CGCGTGAAAG TTCGCCTGAT CATCTTCGAC CGCGAGTCGT CGAACAAGGC GATCAAGGAC ATCAAGGAAC AAGAAGTCTA CATGGGGGAA ATCCCCCTGA TGACCGAGAA CGGTACCTTC ATCATCAACG GTACCGAGCG TGTCATCGTC TCCCAGCTGC ACCGTTCCCC GGGCGTGTTC TTCGACCACG ACCGTGGCAA GACCCACAGC TCCGGCAAGC TGCTGTACTC CGCGCGGATC ATTCCTTACC GCGGTTCCTG GCTGGACTTC GAGTTCGATC CGAAGGACTG CGTGTTCGTC CGTATCGACC GTCGCCGCAA GCTGCCGGCC TCGGTACTGC TGCGCGCGCT CGGCTACAGC ACGGAAGAGA TCCTCAACGC CTTCTACGCG ACCAACGTCT TCCACATCAA GGGCGAGACC CTGAACCTGG AACTGGTCCC GCAGCGCCTG CGCGGTGAAG TCGCGAGCAT CGACATCAAG GATGGCAGCG GCAAGGTGAT CGTGGAGCAG GGGCGTCGTA TCACTGCCCG CCACATCAAC CAGCTGGAAA AGGCTGGCGT GAGCCAGCTG GAAGTGCCGT TCGACTACCT GATCGGCCGT ACCATCGCCA AGGCGATCGT GCATCCGGCT ACCGGCGAGA TCATCGCCGA GTGCAACACC GAGCTGACCC TCGACCTCCT GGCCAAGGTG GCCAAGGCCC AGGTCGTGCG CATCGAGACC CTGTACACCA ACGACATCGA CTGCGGTCCG TTCATCTCCG ACACCCTGAA GATCGACAAC ACCAGCAACC AGCTGGAAGC CCTGGTCGAG ATCTACCGGA TGATGCGTCC GGGCGAGCCG CCGACCAAGG AAGCTGCCGA GACCCTGTTC GGCAACCTGT TCTTCAGCGC CGAGCGTTAC GACCTGTCGG CCGTAGGCCG GATGAAGTTC AACCGCCGTA TCGGTCGTAC CGAGATCGAA GGTCCGGGCG TCCTGAGCAA GGAAGACATC ATCGATGTGC TCAAGACCCT CGTCGACATC CGTAACGGCA AGGGCATCGT CGATGACATC GACCACCTGG GCAACCGTCG TGTCCGTTGC GTCGGCGAAA TGGCCGAGAA CCAGTTCCGC GTGGGCCTGG TGCGTGTCGA GCGCGCGGTC AAGGAACGCC TGTCCATGGC CGAAAGCGAA GGCCTGATGC CGCAAGACCT GATCAACGCC AAGCCGGTGG CTGCCGCGAT CAAGGAGTTC TTCGGTTCGA GCCAGCTGTC GCAGTTCATG GACCAGAACA ACCCGCTTTC CGAGATCACC CACAAGCGCC GCGTCTCCGC GCTCGGCCCG GGCGGTCTGA CCCGTGAGCG TGCGGGCTTC GAGGTTCGTG ACGTACACCC GACCCACTAC GGCCGCGTGT GCCCGATCGA AACCCCTGAA GGTCCGAACA TCGGTCTGAT CAACTCCCTG GCGACCTACG CCCGCACCAA CAAGTACGGC TTCCTCGAGA GCCCGTACCG CGTGGTCAAG GACAGCCTGG TAACCGACGA GATCGTGTTC CTGTCGGCGA TCGAAGAAGC CGACCACGTC ATCGCCCAGG CTTCGGCGAC CCTCAACGAG AAGGGTCAAC TGGTGGACGA GCTGGTGGCC GTGCGTCACC TGAACGAATT CACCGTGAAG GCGCCGGAAG ACGTGACCCT GATGGACGTG TCGCCGAAGC AGGTCGTTTC CGTCGCTGCC TCGCTGATTC CGTTCCTCGA GCACGATGAC GCCAACCGCG CACTCATGGG CTCGAACATG CAGCGTCAGG CCGTGCCGAC CCTGCGTGCC GACAAGCCGC TGGTGGGTAC CGGCATGGAG CGCAACGTGG CGCGCGACTC CGGCGTCTGC GTCGTGGCTC GCCGTGGCGG TGTGATCGAC TCGGTCGATG CCAGCCGTGT CGTGGTTCGC GTGGCGGATG ACGAAGTCGA GACCGGCGAA GCGGGTGTCG ACATCTACAA CCTGACCAAG TACACTCGTT CCAACCAGAA CACCTGCATC AACCAGCGTC CGCTGGTGAG CAAGGGTGAC GTGGTCGCGC GCGGCGACAT CCTGGCCGAC GGTCCGTCCA CCGACATGGG CGAACTGGCC CTGGGCCAGA ACATGCGCGT AGCGTTCATG CCCTGGAACG GCTTCAACTT CGAAGACTCC ATCTGCCTGT CCGAGCGCGT GGTCCAGGAA GATCGTTTCA CCACGATCCA CATCCAGGAA CTGACCTGCG TCGCTCGTGA CACCAAGCTC GGCCCAGAGG AAATCACCGC GGACATCCCG AACGTGGGCG AGGCCGCGCT GAACAAGCTG GACGAAGCCG GTATCGTCTA CGTCGGCGCC GAAGTGCAGG CCGGCGACAT CCTGGTCGGC AAGGTCACTC CGAAAGGCGA GACCCAGCTG ACTCCGGAAG AGAAGCTGCT GCGCGCGATC TTCGGTGAGA AGGCGTCCGA CGTGAAGGAC ACCTCCCTGC GTGTGCCGAC CGGCACCAAG GGTACCGTCA TCGACGTTCA GGTCTTCACC CGCGACGGCG TCGAGCGCGA TTCCCGCGCG CTGTCCATCG AGAAGATGCA ACTCGACCAG ATCCGCAAGG ACCTGAACGA AGAGTTCCGC ATCGTCGAAG GCGCGACCTT CGAGCGTCTG CGTGCCGCCC TGGTCGGTGC CAAGGCTGAA GGTGGCCCGG CGCTGAAGAA GGGCACGGAG ATCACCGACG ACTACCTCGA CGGTCTCGAG CGCGGCCAGT GGTTCAAGCT GCGCATGGCC GACGACGCCC TGAACGAACA GCTGGAGAAG GCCCAGGCCT ACATCAGCGA TCGTCGCCAG CTCCTGGACG ACAAGTTCGA GGACAAGAAG CGCAAGCTGC AGCAGGGCGA CGACCTGGCT CCGGGCGTGC TGAAGATCGT CAAGGTCTAC CTGGCGATCA AGCGTCGCAT CCAGCCGGGC GACAAGATGG CCGGCCGTCA CGGTAACAAG GGTGTGGTCT CGGTGATCAT GCCGGTGGAA GACATGCCGC ACGATGCCAA CGGCACCCCG GTCGACATCG TCCTCAACCC GCTGGGCGTA CCGTCGCGTA TGAACGTCGG TCAGATCCTC GAAACCCACC TGGGCCTCGC GGCCAAGGGG CTGGGCGAGA AGATCAACCG CATGCTCGAA GAGCAGCGCA AGGTCGCCGA ACTGCGTAAG TTCCTGCACG AGATCTACAA CGAGATCGGC GGTCGCGAGG AAAACCTCGA CGAGCTGGGC GACAACGAGA TCCTCGCGCT GGCCAAGAAC CTGCGCGGTG GCGTACCGAT GGCGACCCCG GTGTTCGATG GCGCCAAGGA ACGCGAGATC AAGGCCATGC TGAAGCTGGC CGACCTGCCG GAAAGCGGCC AGATGCGTCT GTTCGACGGC CGTACCGGCA ACCAGTTCGA GCGTCCGACC ACCGTCGGCT ACATGTACAT GCTCAAGCTG AACCACCTGG TGGACGACAA GATGCACGCC CGTTCCACCG GCTCGTACAG CCTGGTTACC CAGCAGCCGC TGGGTGGTAA GGCACAGTTC GGTGGTCAGC GCTTCGGTGA GATGGAGGTG TGGGCGCTGG AAGCCTATGG CGCGGCGTAC ACCCTGCAGG AAATGCTGAC GGTCAAGTCG GACGACGTGA ACGGCCGGAC CAAGATGTAC AAGAACATCG TGGACGGCGA TCACCGCATG GAGGCCGGCA TGCCCGAGTC CTTCAACGTT CTGATCAAAG AGATCCGTTC GCTCGGCATC GACATCGAAC TGGAAACCGA ATAA
Details for PsaeA.18055.a.B1.GE38523
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 152kDa
OBSERVED MW: 150kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA YSYTEKKRIR KDFSKLPDVM DVPYLLAIQL DSYREFLQAG ATKEQFRDVG LHAAFKSVFP IISYSGNAAL EYVGYRLGEP AFDVKECVLR GVTFAVPLRV KVRLIIFDRE SSNKAIKDIK EQEVYMGEIP LMTENGTFII NGTERVIVSQ LHRSPGVFFD HDRGKTHSSG KLLYSARIIP YRGSWLDFEF DPKDCVFVRI DRRRKLPASV LLRALGYSTE EILNAFYATN VFHIKGETLN LELVPQRLRG EVASIDIKDG SGKVIVEQGR RITARHINQL EKAGVSQLEV PFDYLIGRTI AKAIVHPATG EIIAECNTEL TLDLLAKVAK AQVVRIETLY TNDIDCGPFI SDTLKIDNTS NQLEALVEIY RMMRPGEPPT KEAAETLFGN LFFSAERYDL SAVGRMKFNR RIGRTEIEGP GVLSKEDIID VLKTLVDIRN GKGIVDDIDH LGNRRVRCVG EMAENQFRVG LVRVERAVKE RLSMAESEGL MPQDLINAKP VAAAIKEFFG SSQLSQFMDQ NNPLSEITHK RRVSALGPGG LTRERAGFEV RDVHPTHYGR VCPIETPEGP NIGLINSLAT YARTNKYGFL ESPYRVVKDS LVTDEIVFLS AIEEADHVIA QASATLNEKG QLVDELVAVR HLNEFTVKAP EDVTLMDVSP KQVVSVAASL IPFLEHDDAN RALMGSNMQR QAVPTLRADK PLVGTGMERN VARDSGVCVV ARRGGVIDSV DASRVVVRVA DDEVETGEAG VDIYNLTKYT RSNQNTCINQ RPLVSKGDVV ARGDILADGP STDMGELALG QNMRVAFMPW NGFNFEDSIC LSERVVQEDR FTTIHIQELT CVARDTKLGP EEITADIPNV GEAALNKLDE AGIVYVGAEV QAGDILVGKV TPKGETQLTP EEKLLRAIFG EKASDVKDTS LRVPTGTKGT VIDVQVFTRD GVERDSRALS IEKMQLDQIR KDLNEEFRIV EGATFERLRA ALVGAKAEGG PALKKGTEIT DDYLDGLERG QWFKLRMADD ALNEQLEKAQ AYISDRRQLL DDKFEDKKRK LQQGDDLAPG VLKIVKVYLA IKRRIQPGDK MAGRHGNKGV VSVIMPVEDM PHDANGTPVD IVLNPLGVPS RMNVGQILET HLGLAAKGLG EKINRMLEEQ RKVAELRKFL HEIYNEIGGR EENLDELGDN EILALAKNLR GGVPMATPVF DGAKEREIKA MLKLADLPES GQMRLFDGRT GNQFERPTTV GYMYMLKLNH LVDDKMHARS TGSYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL QEMLTVKSDD VNGRTKMYKN IVDGDHRMEA GMPESFNVLI KEIRSLGIDI ELETE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt actcatacac tgagaaaaaa cgtatccgca aggactttag caagttgccg gacgtcatgg atgtgccgta tttgctggcc atccagctgg attcctatcg cgaattcctg caggctggcg caaccaagga gcagttccgc gatgtcggtc tgcacgcggc cttcaagtcc gttttcccga ttatcagcta ttccggcaat gctgccctgg aatacgtcgg ctaccgtctg ggtgagccgg cattcgatgt caaggagtgc gtgctgcgcg gcgtgacctt cgccgtaccg ctgcgcgtga aagttcgcct gatcatcttc gaccgcgagt cgtcgaacaa ggcgatcaag gacatcaagg aacaagaagt ctacatgggg gaaatccccc tgatgaccga gaacggtacc ttcatcatca acggtaccga gcgtgtcatc gtctcccagc tgcaccgttc cccgggcgtg ttcttcgacc acgaccgtgg caagacccac agctccggca agctgctgta ctccgcgcgg atcattcctt accgcggttc ctggctggac ttcgagttcg atccgaagga ctgcgtgttc gtccgtatcg accgtcgccg caagctgccg gcctcggtac tgctgcgcgc gctcggctac agcacggaag agatcctcaa cgccttctac gcgaccaacg tcttccacat caagggcgag accctgaacc tggaactggt cccgcagcgc ctgcgcggtg aagtcgcgag catcgacatc aaggatggca gcggcaaggt gatcgtggag caggggcgtc gtatcactgc ccgccacatc aaccagctgg aaaaggctgg cgtgagccag ctggaagtgc cgttcgacta cctgatcggc cgtaccatcg ccaaggcgat cgtgcatccg gctaccggcg agatcatcgc cgagtgcaac accgagctga ccctcgacct cctggccaag gtggccaagg cccaggtcgt gcgcatcgag accctgtaca ccaacgacat cgactgcggt ccgttcatct ccgacaccct gaagatcgac aacaccagca accagctgga agccctggtc gagatctacc ggatgatgcg tccgggcgag ccgccgacca aggaagctgc cgagaccctg ttcggcaacc tgttcttcag cgccgagcgt tacgacctgt cggccgtagg ccggatgaag ttcaaccgcc gtatcggtcg taccgagatc gaaggtccgg gcgtcctgag caaggaagac atcatcgatg tgctcaagac cctcgtcgac atccgtaacg gcaagggcat cgtcgatgac atcgaccacc tgggcaaccg tcgtgtccgt tgcgtcggcg aaatggccga gaaccagttc cgcgtgggcc tggtgcgtgt cgagcgcgcg gtcaaggaac gcctgtccat ggccgaaagc gaaggcctga tgccgcaaga cctgatcaac gccaagccgg tggctgccgc gatcaaggag ttcttcggtt cgagccagct gtcgcagttc atggaccaga acaacccgct ttccgagatc acccacaagc gccgcgtctc cgcgctcggc ccgggcggtc tgacccgtga gcgtgcgggc ttcgaggttc gtgacgtaca cccgacccac tacggccgcg tgtgcccgat cgaaacccct gaaggtccga acatcggtct gatcaactcc ctggcgacct acgcccgcac caacaagtac ggcttcctcg agagcccgta ccgcgtggtc aaggacagcc tggtaaccga cgagatcgtg ttcctgtcgg cgatcgaaga agccgaccac gtcatcgccc aggcttcggc gaccctcaac gagaagggtc aactggtgga cgagctggtg gccgtgcgtc acctgaacga attcaccgtg aaggcgccgg aagacgtgac cctgatggac gtgtcgccga agcaggtcgt ttccgtcgct gcctcgctga ttccgttcct cgagcacgat gacgccaacc gcgcactcat gggctcgaac atgcagcgtc aggccgtgcc gaccctgcgt gccgacaagc cgctggtggg taccggcatg gagcgcaacg tggcgcgcga ctccggcgtc tgcgtcgtgg ctcgccgtgg cggtgtgatc gactcggtcg atgccagccg tgtcgtggtt cgcgtggcgg atgacgaagt cgagaccggc gaagcgggtg tcgacatcta caacctgacc aagtacactc gttccaacca gaacacctgc atcaaccagc gtccgctggt gagcaagggt gacgtggtcg cgcgcggcga catcctggcc gacggtccgt ccaccgacat gggcgaactg gccctgggcc agaacatgcg cgtagcgttc atgccctgga acggcttcaa cttcgaagac tccatctgcc tgtccgagcg cgtggtccag gaagatcgtt tcaccacgat ccacatccag gaactgacct gcgtcgctcg tgacaccaag ctcggcccag aggaaatcac cgcggacatc ccgaacgtgg gcgaggccgc gctgaacaag ctggacgaag ccggtatcgt ctacgtcggc gccgaagtgc aggccggcga catcctggtc ggcaaggtca ctccgaaagg cgagacccag ctgactccgg aagagaagct gctgcgcgcg atcttcggtg agaaggcgtc cgacgtgaag gacacctccc tgcgtgtgcc gaccggcacc aagggtaccg tcatcgacgt tcaggtcttc acccgcgacg gcgtcgagcg cgattcccgc gcgctgtcca tcgagaagat gcaactcgac cagatccgca aggacctgaa cgaagagttc cgcatcgtcg aaggcgcgac cttcgagcgt ctgcgtgccg ccctggtcgg tgccaaggct gaaggtggcc cggcgctgaa gaagggcacg gagatcaccg acgactacct cgacggtctc gagcgcggcc agtggttcaa gctgcgcatg gccgacgacg ccctgaacga acagctggag aaggcccagg cctacatcag cgatcgtcgc cagctcctgg acgacaagtt cgaggacaag aagcgcaagc tgcagcaggg cgacgacctg gctccgggcg tgctgaagat cgtcaaggtc tacctggcga tcaagcgtcg catccagccg ggcgacaaga tggccggccg tcacggtaac aagggtgtgg tctcggtgat catgccggtg gaagacatgc cgcacgatgc caacggcacc ccggtcgaca tcgtcctcaa cccgctgggc gtaccgtcgc gtatgaacgt cggtcagatc ctcgaaaccc acctgggcct cgcggccaag gggctgggcg agaagatcaa ccgcatgctc gaagagcagc gcaaggtcgc cgaactgcgt aagttcctgc acgagatcta caacgagatc ggcggtcgcg aggaaaacct cgacgagctg ggcgacaacg agatcctcgc gctggccaag aacctgcgcg gtggcgtacc gatggcgacc ccggtgttcg atggcgccaa ggaacgcgag atcaaggcca tgctgaagct ggccgacctg ccggaaagcg gccagatgcg tctgttcgac ggccgtaccg gcaaccagtt cgagcgtccg accaccgtcg gctacatgta catgctcaag ctgaaccacc tggtggacga caagatgcac gcccgttcca ccggctcgta cagcctggtt acccagcagc cgctgggtgg taaggcacag ttcggtggtc agcgcttcgg tgagatggag gtgtgggcgc tggaagccta tggcgcggcg tacaccctgc aggaaatgct gacggtcaag tcggacgacg tgaacggccg gaccaagatg tacaagaaca tcgtggacgg cgatcaccgc atggaggccg gcatgcccga gtccttcaac gttctgatca aagagatccg ttcgctcggc atcgacatcg aactggaaac cgaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.18055.a.B2.GE38524
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 98
Validated AA Sequence
MAXXXXXXXX FXXXPXXXXX PYLLAXQLDS YREFLQAGAT KEQFRDVGLH AAFKSVFPII SYSGNAALEY VGYRLGEPAF DVKECVLRGV TFAVPLRVKV RLIIFDRESS NKAIKDIKEQ EVYMGEIPLM TENGTFIING TERVIVSQLH RSPGVFFDHD RGKTHSSGKL LYSARIIPYR GSWLDFEFDP KDCVFVRIDR RRKLPASVLL RALGYSTEEI LNAFYAXXVF HIKGETLNLX LVPQRLRGEV ASIDIKDGSG KXIVEQGRRI TARHINQLEK AGVSQLEVPF DYLIGRTIAK AIVHPATGEI IAECNTELTL DLLAKVAKAQ VVRIETLYTN DIDCGPFISD TLKIDNTSNQ LEALVEIYRM MRPGEPPTKE AAETLFGNLF FSAERYDLSA VGRMKFNRRI GRTEIEGPGV LSKEDIIDVL KTLVDIRNGK GIVDDIDHLG NRRVRCVGEM AENQFRVGLV RVERAVKERL SMAESEGLMP QDLINAKPVA AAIKEFF
Validated NT Sequence
cgaagaactc cttgatcgcg gcagccaccg gcttggcgtt gatcaggtct tgcggcatca ggccttcgct ttcggccatg gacaggcgtt ccttgaccgc gcgctcgaca cgcaccaggc ccacgcggaa ctggttctcg gccatttcgc cgacgcaacg gacacgacgg ttgcccaggt ggtcgatgtc atcgacgatg cccttgccgt tacggatgtc gacgagggtc ttgagcacat cgatgatgtc ttccttgctc aggacgcccg gaccttcgat ctcggtacga ccgatacggc ggttgaactt catccggcct acggccgaca ggtcgtaacg ctcggcgctg aagaacaggt tgccgaacag ggtctcggca gcttccttgg tcggcggctc gcccggacgc atcatccggt agatctcgac cagggcttcc agctggttgc tggtgttgtc gatcttcagg gtgtcggaga tgaacggacc gcagtcgatg tcgttggtgt acagggtctc gatgcgcacg acctgggcct tggccacctt ggccaggagg tcgagggtca gctcggtgtt gcactcggcg atgatctcgc cggtagccgg atgcacgatc gccttggcga tggtacggcc gatcaggtag tcgaacggca cttccagctg gctcacgcca gccttttcca gctggttgat gtggcgggca gtgatacgac gcccctgctc cacgatcanc ttgccgctgc catccttgat gtcgatgctc gcgacttcac cgcgcaggcg ctgcgggacc agttncaggt tcagggtctc gcccttgatg tggaagacnn nnnncgcgta gaaggcgttg aggatctctt ccgtgctgta gccgagcgcg cgcagcagta ccgaggccgg cagcttgcgg cgacggtcga tacggacgaa cacgcagtcc ttcggatcga actcgaagtc cagccaggaa ccgcggtaag gaatgatccg cgcggagtac agcagcttgc cggagctgtg ggtcttgcca cggtcgtggt cgaagaacac gcccggggaa cggtgcagct gggagacgat gacacgctcg gtaccgttga tgatgaaggt accgttctcg gtcatcaggg ggatttcccc catgtagact tcttgttcct tgatgtcctt gatcgccttg ttcgacgact cgcggtcgaa gatgatcagg cgaactttca cgcgcagcgg tacggcgaag gtcacgccgc gcagcacgca ctccttgaca tcgaatgccg gctcacccag acggtagccg acgtattcca gggcagcatt gccggaatag ctgataatcg ggaaaacgga cttgaaggcc gcgtgcagac cgacatcgcg gaactgctcc ttggttgcgc cagcctgcag gaattcgcga taggaatcca gctgnnnggc cagcaaatac ggcannnccn tnnnnnncgg cannnnncta aagnncnnnn nnnnnnggnn nngnnnagcc at
Expected Protein Sequence
MAHHHHHHDF SKLPDVMDVP YLLAIQLDSY REFLQAGATK EQFRDVGLHA AFKSVFPIIS YSGNAALEYV GYRLGEPAFD VKECVLRGVT FAVPLRVKVR LIIFDRESSN KAIKDIKEQE VYMGEIPLMT ENGTFIINGT ERVIVSQLHR SPGVFFDHDR GKTHSSGKLL YSARIIPYRG SWLDFEFDPK DCVFVRIDRR RKLPASVLLR ALGYSTEEIL NAFYATNVFH IKGETLNLEL VPQRLRGEVA SIDIKDGSGK VIVEQGRRIT ARHINQLEKA GVSQLEVPFD YLIGRTIAKA IVHPATGEII AECNTELTLD LLAKVAKAQV VRIETLYTND IDCGPFISDT LKIDNTSNQL EALVEIYRMM RPGEPPTKEA AETLFGNLFF SAERYDLSAV GRMKFNRRIG RTEIEGPGVL SKEDIIDVLK TLVDIRNGKG IVDDIDHLGN RRVRCVGEMA ENQFRVGLVR VERAVKERLS MAESEGLMPQ DLINAKPVAA AIKEFFGSSQ LSQF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacttta gcaagttgcc ggacgtcatg gatgtgccgt atttgctggc catccagctg gattcctatc gcgaattcct gcaggctggc gcaaccaagg agcagttccg cgatgtcggt ctgcacgcgg ccttcaagtc cgttttcccg attatcagct attccggcaa tgctgccctg gaatacgtcg gctaccgtct gggtgagccg gcattcgatg tcaaggagtg cgtgctgcgc ggcgtgacct tcgccgtacc gctgcgcgtg aaagttcgcc tgatcatctt cgaccgcgag tcgtcgaaca aggcgatcaa ggacatcaag gaacaagaag tctacatggg ggaaatcccc ctgatgaccg agaacggtac cttcatcatc aacggtaccg agcgtgtcat cgtctcccag ctgcaccgtt ccccgggcgt gttcttcgac cacgaccgtg gcaagaccca cagctccggc aagctgctgt actccgcgcg gatcattcct taccgcggtt cctggctgga cttcgagttc gatccgaagg actgcgtgtt cgtccgtatc gaccgtcgcc gcaagctgcc ggcctcggta ctgctgcgcg cgctcggcta cagcacggaa gagatcctca acgccttcta cgcgaccaac gtcttccaca tcaagggcga gaccctgaac ctggaactgg tcccgcagcg cctgcgcggt gaagtcgcga gcatcgacat caaggatggc agcggcaagg tgatcgtgga gcaggggcgt cgtatcactg cccgccacat caaccagctg gaaaaggctg gcgtgagcca gctggaagtg ccgttcgact acctgatcgg ccgtaccatc gccaaggcga tcgtgcatcc ggctaccggc gagatcatcg ccgagtgcaa caccgagctg accctcgacc tcctggccaa ggtggccaag gcccaggtcg tgcgcatcga gaccctgtac accaacgaca tcgactgcgg tccgttcatc tccgacaccc tgaagatcga caacaccagc aaccagctgg aagccctggt cgagatctac cggatgatgc gtccgggcga gccgccgacc aaggaagctg ccgagaccct gttcggcaac ctgttcttca gcgccgagcg ttacgacctg tcggccgtag gccggatgaa gttcaaccgc cgtatcggtc gtaccgagat cgaaggtccg ggcgtcctga gcaaggaaga catcatcgat gtgctcaaga ccctcgtcga catccgtaac ggcaagggca tcgtcgatga catcgaccac ctgggcaacc gtcgtgtccg ttgcgtcggc gaaatggccg agaaccagtt ccgcgtgggc ctggtgcgtg tcgagcgcgc ggtcaaggaa cgcctgtcca tggccgaaag cgaaggcctg atgccgcaag acctgatcaa cgccaagccg gtggctgccg cgatcaagga gttcttcggt tcgagccagc tgtcgcagtt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for PsaeA.18055.a.B3.GE38525
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM EDRFTTIHIQ ELTCVARDTK LGPEEITADI PNVGEAALNK LDEAGIVYVG AEVQAGDILV GKVTPKGETQ LTPEEKLLRA IFGEKASDVK DTSLRVPTGT KGTVIDVQVF TRDGVERDSR ALSIEKMQLD QIRKDLNEEF RIVEGATFER LRAALVGAKA EGGPALKKGT EITDDYLDGL ERGQWFKLRM ADDALNEQLE KAQAYISDRR QLLDDKFEDK KRKLQQGDDL APGVLKIVKV YLAIKRXIQP GD
Validated NT Sequence
atcttactca cttagtcgcc cggctggatg nnncgcttga tcgccaggta gaccttgacg atcttcagca cgcccggagc caggtcgtcg ccctgctgca gcttgcgctt cttgtcctcg aacttgtcgt ccaggagctg gcgacgatcg ctgatgtagg cctgggcctt ctccagctgt tcgttcaggg cgtcgtcggc catgcgcagc ttgaaccact ggccgcgctc gagaccgtcg aggtagtcgt cggtgatctc cgtgcccttc ttcagcgccg ggccaccttc agccttggca ccgaccaggg cggcacgcag acgctcgaag gtcgcgcctt cgacgatgcg gaactcttcg ttcaggtcct tgcggatctg gtcgagttgc atcttctcga tggacagcgc gcgggaatcg cgctcgacgc cgtcgcgggt gaagacctga acgtcgatga cggtaccctt ggtgccggtc ggcacacgca gggaggtgtc cttcacgtcg gacgccttct caccgaagat cgcgcgcagc agcttctctt ccggagtcag ctgggtctcg cctttcggag tgaccttgcc gaccaggatg tcgccggcct gcacttcggc gccgacgtag acgataccgg cttcgtccag cttgttcagc gcggcctcgc ccacgttcgg gatgtccgcg gtgatttcct ctgggccgag cttggtgtca cgagcgacgc aggtcagttc ctggatgtgg atcgtggtga aacgatcttc catatggtgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHED RFTTIHIQEL TCVARDTKLG PEEITADIPN VGEAALNKLD EAGIVYVGAE VQAGDILVGK VTPKGETQLT PEEKLLRAIF GEKASDVKDT SLRVPTGTKG TVIDVQVFTR DGVERDSRAL SIEKMQLDQI RKDLNEEFRI VEGATFERLR AALVGAKAEG GPALKKGTEI TDDYLDGLER GQWFKLRMAD DALNEQLEKA QAYISDRRQL LDDKFEDKKR KLQQGDDLAP GVLKIVKVYL AIKRRIQPGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagatc gtttcaccac gatccacatc caggaactga cctgcgtcgc tcgtgacacc aagctcggcc cagaggaaat caccgcggac atcccgaacg tgggcgaggc cgcgctgaac aagctggacg aagccggtat cgtctacgtc ggcgccgaag tgcaggccgg cgacatcctg gtcggcaagg tcactccgaa aggcgagacc cagctgactc cggaagagaa gctgctgcgc gcgatcttcg gtgagaaggc gtccgacgtg aaggacacct ccctgcgtgt gccgaccggc accaagggta ccgtcatcga cgttcaggtc ttcacccgcg acggcgtcga gcgcgattcc cgcgcgctgt ccatcgagaa gatgcaactc gaccagatcc gcaaggacct gaacgaagag ttccgcatcg tcgaaggcgc gaccttcgag cgtctgcgtg ccgccctggt cggtgccaag gctgaaggtg gcccggcgct gaagaagggc acggagatca ccgacgacta cctcgacggt ctcgagcgcg gccagtggtt caagctgcgc atggccgacg acgccctgaa cgaacagctg gagaaggccc aggcctacat cagcgatcgt cgccagctcc tggacgacaa gttcgaggac aagaagcgca agctgcagca gggcgacgac ctggctccgg gcgtgctgaa gatcgtcaag gtctacctgg cgatcaagcg tcgcatccag ccgggcgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for PsaeA.18055.a.B4.GE38526
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHCV VARRGGVIDS VDASRVVVRV ADDEVETGEA GVDIYNLTKY TRSNQNTCIN QRPLVSKGDV VARGDILADG PSTDMGELAL GQNMRVAFMP WNGFNFEDSI CLSERVVQED RFTTIHIQEL TCVARDTKLG PEEITADIPN VGEAALNKLD EAGIVYVGAE VQAGDILVGK VTPKGETQLT PEEKLLRAIF GEKASDVKDT SLRVPTGTKG TVIDVQVFTR DGVERDSRAL SIEKMQLDQI RKDLNEEFRI VEGATFERLR AALVGAKAEG GPALKKGTEI TDDYLDGLER GQWFKLRMAD DALNEQLEKA QAYISDRRQL LDDKFEDKKR KLQQGDDLAP GVLKIVKVYL AIKRRIQPGD KMAGRHGNKG VVSVIMPVED MPHDANGTPV DIVLNPLGVP SRMNVGQILE THLGLAAKGL GEKINRMLEE QRKVAELRKF LHEIYNEIGG REENLDELGD NEILALAKNL RGGVPMATPV FDGAKEREIK AMLKLADLPE SGQMRLFDGR TGNQFERPTT VGYMYMLKLN HLVDDKMHAR STGSYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT LQEMLTVKSD DVNGRTKMYK NIVDGDHRME AGMPESFNVL IKEIRSLGID IELET
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgcgtcg tggctcgccg tggcggtgtg atcgactcgg tcgatgccag ccgtgtcgtg gttcgcgtgg cggatgacga agtcgagacc ggcgaagcgg gtgtcgacat ctacaacctg accaagtaca ctcgttccaa ccagaacacc tgcatcaacc agcgtccgct ggtgagcaag ggtgacgtgg tcgcgcgcgg cgacatcctg gccgacggtc cgtccaccga catgggcgaa ctggccctgg gccagaacat gcgcgtagcg ttcatgccct ggaacggctt caacttcgaa gactccatct gcctgtccga gcgcgtggtc caggaagatc gtttcaccac gatccacatc caggaactga cctgcgtcgc tcgtgacacc aagctcggcc cagaggaaat caccgcggac atcccgaacg tgggcgaggc cgcgctgaac aagctggacg aagccggtat cgtctacgtc ggcgccgaag tgcaggccgg cgacatcctg gtcggcaagg tcactccgaa aggcgagacc cagctgactc cggaagagaa gctgctgcgc gcgatcttcg gtgagaaggc gtccgacgtg aaggacacct ccctgcgtgt gccgaccggc accaagggta ccgtcatcga cgttcaggtc ttcacccgcg acggcgtcga gcgcgattcc cgcgcgctgt ccatcgagaa gatgcaactc gaccagatcc gcaaggacct gaacgaagag ttccgcatcg tcgaaggcgc gaccttcgag cgtctgcgtg ccgccctggt cggtgccaag gctgaaggtg gcccggcgct gaagaagggc acggagatca ccgacgacta cctcgacggt ctcgagcgcg gccagtggtt caagctgcgc atggccgacg acgccctgaa cgaacagctg gagaaggccc aggcctacat cagcgatcgt cgccagctcc tggacgacaa gttcgaggac aagaagcgca agctgcagca gggcgacgac ctggctccgg gcgtgctgaa gatcgtcaag gtctacctgg cgatcaagcg tcgcatccag ccgggcgaca agatggccgg ccgtcacggt aacaagggtg tggtctcggt gatcatgccg gtggaagaca tgccgcacga tgccaacggc accccggtcg acatcgtcct caacccgctg ggcgtaccgt cgcgtatgaa cgtcggtcag atcctcgaaa cccacctggg cctcgcggcc aaggggctgg gcgagaagat caaccgcatg ctcgaagagc agcgcaaggt cgccgaactg cgtaagttcc tgcacgagat ctacaacgag atcggcggtc gcgaggaaaa cctcgacgag ctgggcgaca acgagatcct cgcgctggcc aagaacctgc gcggtggcgt accgatggcg accccggtgt tcgatggcgc caaggaacgc gagatcaagg ccatgctgaa gctggccgac ctgccggaaa gcggccagat gcgtctgttc gacggccgta ccggcaacca gttcgagcgt ccgaccaccg tcggctacat gtacatgctc aagctgaacc acctggtgga cgacaagatg cacgcccgtt ccaccggctc gtacagcctg gttacccagc agccgctggg tggtaaggca cagttcggtg gtcagcgctt cggtgagatg gaggtgtggg cgctggaagc ctatggcgcg gcgtacaccc tgcaggaaat gctgacggtc aagtcggacg acgtgaacgg ccggaccaag atgtacaaga acatcgtgga cggcgatcac cgcatggagg ccggcatgcc cgagtccttc aacgttctga tcaaagagat ccgttcgctc ggcatcgaca tcgaactgga aacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.18055.a.B2.PS02307
PURIFICATION DATe: 1/20/2015
CONCENTRATION: 71.92mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAXXXXXXXX FXXXPXXXXX PYLLAXQLDS YREFLQAGAT KEQFRDVGLH AAFKSVFPII SYSGNAALEY VGYRLGEPAF DVKECVLRGV TFAVPLRVKV RLIIFDRESS NKAIKDIKEQ EVYMGEIPLM TENGTFIING TERVIVSQLH RSPGVFFDHD RGKTHSSGKL LYSARIIPYR GSWLDFEFDP KDCVFVRIDR RRKLPASVLL RALGYSTEEI LNAFYAXXVF HIKGETLNLX LVPQRLRGEV ASIDIKDGSG KXIVEQGRRI TARHINQLEK AGVSQLEVPF DYLIGRTIAK AIVHPATGEI IAECNTELTL DLLAKVAKAQ VVRIETLYTN DIDCGPFISD TLKIDNTSNQ LEALVEIYRM MRPGEPPTKE AAETLFGNLF FSAERYDLSA VGRMKFNRRI GRTEIEGPGV LSKEDIIDVL KTLVDIRNGK GIVDDIDHLG NRRVRCVGEM AENQFRVGLV RVERAVKERL SMAESEGLMP QDLINAKPVA AAIKEFF
Validated NT Sequence
cgaagaactc cttgatcgcg gcagccaccg gcttggcgtt gatcaggtct tgcggcatca ggccttcgct ttcggccatg gacaggcgtt ccttgaccgc gcgctcgaca cgcaccaggc ccacgcggaa ctggttctcg gccatttcgc cgacgcaacg gacacgacgg ttgcccaggt ggtcgatgtc atcgacgatg cccttgccgt tacggatgtc gacgagggtc ttgagcacat cgatgatgtc ttccttgctc aggacgcccg gaccttcgat ctcggtacga ccgatacggc ggttgaactt catccggcct acggccgaca ggtcgtaacg ctcggcgctg aagaacaggt tgccgaacag ggtctcggca gcttccttgg tcggcggctc gcccggacgc atcatccggt agatctcgac cagggcttcc agctggttgc tggtgttgtc gatcttcagg gtgtcggaga tgaacggacc gcagtcgatg tcgttggtgt acagggtctc gatgcgcacg acctgggcct tggccacctt ggccaggagg tcgagggtca gctcggtgtt gcactcggcg atgatctcgc cggtagccgg atgcacgatc gccttggcga tggtacggcc gatcaggtag tcgaacggca cttccagctg gctcacgcca gccttttcca gctggttgat gtggcgggca gtgatacgac gcccctgctc cacgatcanc ttgccgctgc catccttgat gtcgatgctc gcgacttcac cgcgcaggcg ctgcgggacc agttncaggt tcagggtctc gcccttgatg tggaagacnn nnnncgcgta gaaggcgttg aggatctctt ccgtgctgta gccgagcgcg cgcagcagta ccgaggccgg cagcttgcgg cgacggtcga tacggacgaa cacgcagtcc ttcggatcga actcgaagtc cagccaggaa ccgcggtaag gaatgatccg cgcggagtac agcagcttgc cggagctgtg ggtcttgcca cggtcgtggt cgaagaacac gcccggggaa cggtgcagct gggagacgat gacacgctcg gtaccgttga tgatgaaggt accgttctcg gtcatcaggg ggatttcccc catgtagact tcttgttcct tgatgtcctt gatcgccttg ttcgacgact cgcggtcgaa gatgatcagg cgaactttca cgcgcagcgg tacggcgaag gtcacgccgc gcagcacgca ctccttgaca tcgaatgccg gctcacccag acggtagccg acgtattcca gggcagcatt gccggaatag ctgataatcg ggaaaacgga cttgaaggcc gcgtgcagac cgacatcgcg gaactgctcc ttggttgcgc cagcctgcag gaattcgcga taggaatcca gctgnnnggc cagcaaatac ggcannnccn tnnnnnncgg cannnnncta aagnncnnnn nnnnnnggnn nngnnnagcc at
Expressed Protein Sequence
MAHHHHHHDF SKLPDVMDVP YLLAIQLDSY REFLQAGATK EQFRDVGLHA AFKSVFPIIS YSGNAALEYV GYRLGEPAFD VKECVLRGVT FAVPLRVKVR LIIFDRESSN KAIKDIKEQE VYMGEIPLMT ENGTFIINGT ERVIVSQLHR SPGVFFDHDR GKTHSSGKLL YSARIIPYRG SWLDFEFDPK DCVFVRIDRR RKLPASVLLR ALGYSTEEIL NAFYATNVFH IKGETLNLEL VPQRLRGEVA SIDIKDGSGK VIVEQGRRIT ARHINQLEKA GVSQLEVPFD YLIGRTIAKA IVHPATGEII AECNTELTLD LLAKVAKAQV VRIETLYTND IDCGPFISDT LKIDNTSNQL EALVEIYRMM RPGEPPTKEA AETLFGNLFF SAERYDLSAV GRMKFNRRIG RTEIEGPGVL SKEDIIDVLK TLVDIRNGKG IVDDIDHLGN RRVRCVGEMA ENQFRVGLVR VERAVKERLS MAESEGLMPQ DLINAKPVAA AIKEFFGSSQ LSQF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACTTTA GCAAGTTGCC GGACGTCATG GATGTGCCGT ATTTGCTGGC CATCCAGCTG GATTCCTATC GCGAATTCCT GCAGGCTGGC GCAACCAAGG AGCAGTTCCG CGATGTCGGT CTGCACGCGG CCTTCAAGTC CGTTTTCCCG ATTATCAGCT ATTCCGGCAA TGCTGCCCTG GAATACGTCG GCTACCGTCT GGGTGAGCCG GCATTCGATG TCAAGGAGTG CGTGCTGCGC GGCGTGACCT TCGCCGTACC GCTGCGCGTG AAAGTTCGCC TGATCATCTT CGACCGCGAG TCGTCGAACA AGGCGATCAA GGACATCAAG GAACAAGAAG TCTACATGGG GGAAATCCCC CTGATGACCG AGAACGGTAC CTTCATCATC AACGGTACCG AGCGTGTCAT CGTCTCCCAG CTGCACCGTT CCCCGGGCGT GTTCTTCGAC CACGACCGTG GCAAGACCCA CAGCTCCGGC AAGCTGCTGT ACTCCGCGCG GATCATTCCT TACCGCGGTT CCTGGCTGGA CTTCGAGTTC GATCCGAAGG ACTGCGTGTT CGTCCGTATC GACCGTCGCC GCAAGCTGCC GGCCTCGGTA CTGCTGCGCG CGCTCGGCTA CAGCACGGAA GAGATCCTCA ACGCCTTCTA CGCGACCAAC GTCTTCCACA TCAAGGGCGA GACCCTGAAC CTGGAACTGG TCCCGCAGCG CCTGCGCGGT GAAGTCGCGA GCATCGACAT CAAGGATGGC AGCGGCAAGG TGATCGTGGA GCAGGGGCGT CGTATCACTG CCCGCCACAT CAACCAGCTG GAAAAGGCTG GCGTGAGCCA GCTGGAAGTG CCGTTCGACT ACCTGATCGG CCGTACCATC GCCAAGGCGA TCGTGCATCC GGCTACCGGC GAGATCATCG CCGAGTGCAA CACCGAGCTG ACCCTCGACC TCCTGGCCAA GGTGGCCAAG GCCCAGGTCG TGCGCATCGA GACCCTGTAC ACCAACGACA TCGACTGCGG TCCGTTCATC TCCGACACCC TGAAGATCGA CAACACCAGC AACCAGCTGG AAGCCCTGGT CGAGATCTAC CGGATGATGC GTCCGGGCGA GCCGCCGACC AAGGAAGCTG CCGAGACCCT GTTCGGCAAC CTGTTCTTCA GCGCCGAGCG TTACGACCTG TCGGCCGTAG GCCGGATGAA GTTCAACCGC CGTATCGGTC GTACCGAGAT CGAAGGTCCG GGCGTCCTGA GCAAGGAAGA CATCATCGAT GTGCTCAAGA CCCTCGTCGA CATCCGTAAC GGCAAGGGCA TCGTCGATGA CATCGACCAC CTGGGCAACC GTCGTGTCCG TTGCGTCGGC GAAATGGCCG AGAACCAGTT CCGCGTGGGC CTGGTGCGTG TCGAGCGCGC GGTCAAGGAA CGCCTGTCCA TGGCCGAAAG CGAAGGCCTG ATGCCGCAAG ACCTGATCAA CGCCAAGCCG GTGGCTGCCG CGATCAAGGA GTTCTTCGGT TCGAGCCAGC TGTCGCAGTT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT