PsaeA.17999.a
Adenylosuccinate lyase (ASL) (EC 4.3.2.2) (Adenylosuccinase) (ASase)
| CENTER ID: | PsaeA.17999.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17999.a.B1.GE43997 | full length | 1 | 456 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7T24
DEPOSITED: 12/3/2021
DETERMINATION: XRay
CLONE: PsaeA.17999.a.B1.GE43997
PROTEIN: PsaeA.17999.a.B1.PW38913
7T29
DEPOSITED: 12/3/2021
DETERMINATION: XRay
CLONE: PsaeA.17999.a.B1.GE43997
PROTEIN: PsaeA.17999.a.B1.PW38925
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.2751 |
| PGAT: | pseudomonas:PA2629 |
| RefSeq: | NP_251319.1 |
| UniProt: | Q9I0K9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLSSLTAVS PVDGRYAGKT SSLRPIFSEY GLIRFRVMVE VRWLQRLAAH AGIPEVAPFS AEANALLDSL ASDFQLEHAE RIKEIERTTN HDVKAVEYLL KEQAAKLPEL AAVSEFIHFA CTSEDINNLS HALMLREGRD SVLLPLMRQI AEAIRELAVK LADVPMLSRT HGQPASPTTL GKELANVVYR LERQIKQVAG IELLGKINGA VGNYNAHLSA YPEVDWEANA RQFIEGDLGL TFNPYTTQIE PHDYIAELFD AIARFNTILI DFDRDVWGYI SLGYFKQKTV AGEIGSSTMP HKVNPIDFEN SEGNLGIANA LFQHLASKLP ISRWQRDLTD STVLRNLGVG IAHSIIAYEA SLKGIGKLEL NAQRIAEDLD ACWEVLAEPV QTVMRRYGVE NPYEKLKELT RGKGISAEAL QTFIEELAIP AEAKVELKKL TPAGYVGNAA AQAKRI
NT Sequence
atgcagcttt cctcgctcac cgcggtatcc cccgtcgatg gccgctatgc cggcaaaacc agcagcctgc gtccgatctt cagcgaatac ggcctgatcc gtttccgcgt catggtggaa gtccgctggc tgcagcgcct cgccgcccac gccgggattc ccgaggtcgc gccgttctcc gccgaagcca acgccctgct cgacagcctg gccagcgact tccagctgga acacgccgaa cgcatcaagg aaatcgagcg caccaccaac cacgacgtga aagccgtgga gtacctgctc aaggaacagg ccgccaagct tccggagctg gccgcggtca gcgagttcat ccacttcgcc tgcacctccg aggacatcaa caacctgtcc cacgccctga tgctccgcga gggtcgcgac agcgtgctgc tgccgctgat gcgccagatc gccgaggcca tccgcgaact ggcggtgaaa ctcgccgacg tgccgatgct ctcccgcacc cacggccagc ctgcctcgcc gaccaccctg ggcaaggaac tggccaacgt cgtctaccgc ctggagcggc agatcaagca ggtcgccggc atcgagttgc tgggcaagat caacggtgcc gtgggcaact acaacgccca cctgtccgcc tatcccgaag tcgactggga agccaacgcg cgccagttca tcgaaggcga cctcggcctg accttcaacc cctacaccac tcagatcgag ccgcacgact acatcgccga gctgttcgat gcgatcgccc gcttcaacac catcctcatc gacttcgacc gcgacgtctg gggctacatc tccctcggct atttcaagca gaagaccgtg gccggcgaga tcggctcctc gaccatgccg cacaaggtca acccgatcga cttcgagaac tccgagggca acctcggcat cgccaacgcg ctgttccagc acctggccag caagctgccg atctcgcgct ggcagcgcga cctgaccgac tccaccgtgc tgcgcaacct cggcgtcggc atcgcccaca gcatcatcgc ctacgaggcc agcctgaagg gcatcggcaa gctggagctg aacgcccagc gcatcgccga ggacctggac gcctgctggg aagtcctcgc cgagccggtg cagaccgtga tgcgtcgcta tggcgtggaa aacccctacg agaagctgaa ggaactgacc cgcggcaaag gcatcagcgc cgaagccttg cagactttca tcgaagagct ggcgattccc gccgaggcca aggtcgagct gaagaagctg accccggccg gctacgtcgg caatgccgcc gcccaggcca agcgcatctg a
Details for PsaeA.17999.a.B1.GE43997
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ LSSLTAVSPV DGRYAGKTSS LRPIFSEYGL IRFRVMVEVR WLQRLAAHAG IPEVAPFSAE ANALLDSLAS DFQLEHAERI KEIERTTNHD VKAVEYLLKE QAAKLPELAA VSEFIHFACT SEDINNLSHA LMLREGRDSV LLPLMRQIAE AIRELAVKLA DVPMLSRTHG QPASPTTLGK ELANVVYRLE RQIKQVAGIE LLGKINGAVG NYNAHLSAYP EVDWEANARQ FIEGDLGLTF NPYTTQIEPH DYIAELFDAI ARFNTILIDF DRDVWGYISL GYFKQKTVAG EIGSSTMPHK VNPIDFENSE GNLGIANALF QHLASKLPIS RWQRDLTDST VLRNLGVGIA HSIIAYEASL KGIGKLELNA QRIAEDLDAC WEVLAEPVQT VMRRYGVENP YEKLKELTRG KGISAEALQT FIEELAIPAE AKVELKKLTP AGYVGNAAAQ AKRI
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgca gctttcctcg ctcaccgcgg tatcccccgt cgatggccgc tatgccggca aaaccagcag cctgcgtccg atcttcagcg aatacggcct gatccgtttc cgcgtcatgg tggaagtccg ctggctgcag cgcctcgccg cccacgccgg gattcccgag gtcgcgccgt tctccgccga agccaacgcc ctgctcgaca gcctggccag cgacttccag ctggaacacg ccgaacgcat caaggaaatc gagcgcacca ccaaccacga cgtgaaagcc gtggagtacc tgctcaagga acaggccgcc aagcttccgg agctggccgc ggtcagcgag ttcatccact tcgcctgcac ctccgaggac atcaacaacc tgtcccacgc cctgatgctc cgcgagggtc gcgacagcgt gctgctgccg ctgatgcgcc agatcgccga ggccatccgc gaactggcgg tgaaactcgc cgacgtgccg atgctctccc gcacccacgg ccagcctgcc tcgccgacca ccctgggcaa ggaactggcc aacgtcgtct accgcctgga gcggcagatc aagcaggtcg ccggcatcga gttgctgggc aagatcaacg gtgccgtggg caactacaac gcccacctgt ccgcctatcc cgaagtcgac tgggaagcca acgcgcgcca gttcatcgaa ggcgacctcg gcctgacctt caacccctac accactcaga tcgagccgca cgactacatc gccgagctgt tcgatgcgat cgcccgcttc aacaccatcc tcatcgactt cgaccgcgac gtctggggct acatctccct cggctatttc aagcagaaga ccgtggccgg cgagatcggc tcctcgacca tgccgcacaa ggtcaacccg atcgacttcg agaactccga gggcaacctc ggcatcgcca acgcgctgtt ccagcacctg gccagcaagc tgccgatctc gcgctggcag cgcgacctga ccgactccac cgtgctgcgc aacctcggcg tcggcatcgc ccacagcatc atcgcctacg aggccagcct gaagggcatc ggcaagctgg agctgaacgc ccagcgcatc gccgaggacc tggacgcctg ctgggaagtc ctcgccgagc cggtgcagac cgtgatgcgt cgctatggcg tggaaaaccc ctacgagaag ctgaaggaac tgacccgcgg caaaggcatc agcgccgaag ccttgcagac tttcatcgaa gagctggcga ttcccgccga ggccaaggtc gagctgaaga agctgacccc ggccggctac gtcggcaatg ccgccgccca ggccaagcgc atctaagtga gtaagatagg atccggctgc taacaaagcc cgaaagaa
Expected Protein Sequence
MAHHHHHHMQ LSSLTAVSPV DGRYAGKTSS LRPIFSEYGL IRFRVMVEVR WLQRLAAHAG IPEVAPFSAE ANALLDSLAS DFQLEHAERI KEIERTTNHD VKAVEYLLKE QAAKLPELAA VSEFIHFACT SEDINNLSHA LMLREGRDSV LLPLMRQIAE AIRELAVKLA DVPMLSRTHG QPASPTTLGK ELANVVYRLE RQIKQVAGIE LLGKINGAVG NYNAHLSAYP EVDWEANARQ FIEGDLGLTF NPYTTQIEPH DYIAELFDAI ARFNTILIDF DRDVWGYISL GYFKQKTVAG EIGSSTMPHK VNPIDFENSE GNLGIANALF QHLASKLPIS RWQRDLTDST VLRNLGVGIA HSIIAYEASL KGIGKLELNA QRIAEDLDAC WEVLAEPVQT VMRRYGVENP YEKLKELTRG KGISAEALQT FIEELAIPAE AKVELKKLTP AGYVGNAAAQ AKRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc tttcctcgct caccgcggta tcccccgtcg atggccgcta tgccggcaaa accagcagcc tgcgtccgat cttcagcgaa tacggcctga tccgtttccg cgtcatggtg gaagtccgct ggctgcagcg cctcgccgcc cacgccggga ttcccgaggt cgcgccgttc tccgccgaag ccaacgccct gctcgacagc ctggccagcg acttccagct ggaacacgcc gaacgcatca aggaaatcga gcgcaccacc aaccacgacg tgaaagccgt ggagtacctg ctcaaggaac aggccgccaa gcttccggag ctggccgcgg tcagcgagtt catccacttc gcctgcacct ccgaggacat caacaacctg tcccacgccc tgatgctccg cgagggtcgc gacagcgtgc tgctgccgct gatgcgccag atcgccgagg ccatccgcga actggcggtg aaactcgccg acgtgccgat gctctcccgc acccacggcc agcctgcctc gccgaccacc ctgggcaagg aactggccaa cgtcgtctac cgcctggagc ggcagatcaa gcaggtcgcc ggcatcgagt tgctgggcaa gatcaacggt gccgtgggca actacaacgc ccacctgtcc gcctatcccg aagtcgactg ggaagccaac gcgcgccagt tcatcgaagg cgacctcggc ctgaccttca acccctacac cactcagatc gagccgcacg actacatcgc cgagctgttc gatgcgatcg cccgcttcaa caccatcctc atcgacttcg accgcgacgt ctggggctac atctccctcg gctatttcaa gcagaagacc gtggccggcg agatcggctc ctcgaccatg ccgcacaagg tcaacccgat cgacttcgag aactccgagg gcaacctcgg catcgccaac gcgctgttcc agcacctggc cagcaagctg ccgatctcgc gctggcagcg cgacctgacc gactccaccg tgctgcgcaa cctcggcgtc ggcatcgccc acagcatcat cgcctacgag gccagcctga agggcatcgg caagctggag ctgaacgccc agcgcatcgc cgaggacctg gacgcctgct gggaagtcct cgccgagccg gtgcagaccg tgatgcgtcg ctatggcgtg gaaaacccct acgagaagct gaaggaactg acccgcggca aaggcatcag cgccgaagcc ttgcagactt tcatcgaaga gctggcgatt cccgccgagg ccaaggtcga gctgaagaag ctgaccccgg ccggctacgt cggcaatgcc gccgcccagg ccaagcgcat ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat