SSGCID: Target Page

PsaeA.17988.a

ATP synthase gamma chain (ATP synthase F1 sector gamma subunit) (F-ATPase gamma subunit)

CENTER ID:	PsaeA.17988.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17988.a.B1.GE38516	full length		1	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.5821
PGAT:	pseudomonas:PA5555
RefSeq:	NP_254242.1
UniProt:	Q9HT19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGAKEIRSK IASIKSTQKI TNAMEKVAVS KMRKAQMRMA AGRPYAERIR QVIGHLANAN PEYRHPFMVE REVKRVGYIV VSSDRGLCGG LNINLFKSLV KDMSGYREQG AEIDLCVIGS KGASFFRSFG GNVVAAISHL GEEPSINDLI GSVKVMLDAY LEGRIDRLFV VSNKFVNTMT QKPTVEQLIP LVADDDQELK HHWDYLYEPD AKSLLDGLLV RYVESQVYQA VVENNACEQA ARMIAMKNAT DNAGELISDL QLIYNKARQA AITQEISEIV GGAAAV

NT Sequence

atggcaggcg caaaagagat tcgcagcaag attgcgagca tcaaaagcac gcaaaagatc accaatgcca tggaaaaagt ggcggtgagc aagatgcgca aggcacaaat gcgcatggcg gccggccgtc cctacgcgga gcgtattcgc caggtgatcg gccatctggc caacgccaac ccggaatacc gtcacccgtt catggtcgag cgtgaagtca agcgcgtcgg ctacatcgtg gtgagcagtg accgtggtct gtgcggcggt ctgaacatca acctgttcaa gtccctcgtc aaggacatga gcggctaccg cgagcaaggg gcggaaatcg acctttgcgt gatcggtagc aagggcgctt cgttcttccg cagtttcggc ggcaacgtcg tggcagctat cagccacctc ggcgaagagc cttcgatcaa cgacctgatc ggcagtgtca aggtgatgct cgacgcatac ctcgaaggcc gtatcgatcg cctgttcgtg gtctccaaca agttcgtcaa caccatgacc cagaagccga ccgtggaaca gctgattccg ctggtggccg atgacgatca ggagctgaag caccactggg actatctcta cgaacccgac gccaagtcgc tcctcgacgg gctgctggtg cgttacgtgg aatcccaggt gtaccaggcc gtggttgaga acaacgcctg tgagcaggcg gcccggatga ttgcaatgaa gaacgctacc gacaacgccg gcgagctgat cagcgatttg cagctgatct acaacaaggc gcgtcaggcg gcgatcaccc aggaaatctc ggaaatcgtc ggcggcgctg ccgcggtgta a

Details for PsaeA.17988.a.B1.GE38516

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA GAKEIRSKIA SIKSTQKITN AMEKVAVSKM RKAQMRMAAG RPYAERIRQV IGHLANANPE YRHPFMVERE VKRVGYIVVS SDRGLCGGLN INLFKSLVKD MSGYREQGAE IDLCVIGSKG ASFFRSFGGN VVAAISHLGE EPSINDLIGS VKVMLDAYLE GRIDRLFVVS NKFVNTMTQK PTVEQLIPLV ADDDQELKHH WDYLYEPDAK SLLDGLLVRY VESQVYQAVV ENNACEQAAR MIAMKNATDN AGELISDLQL IYNKARQAAI TQEISE

Validated NT Sequence

tttccgagat ttcctgggtg atcgccgcct gacgcgcctt gttgtagatc agctgcaaat cgctgatcag ctcgccggcg ttgtcggtag cgttcttcat tgcaatcatc cgggccgcct gctcacaggc gttgttctca accacggcct ggtacacctg ggattccacg taacgcacca gcagcccgtc gaggagcgac ttggcgtcgg gttcgtagag atagtcccag tggtgcttca gctcctgatc gtcatcggcc accagcggaa tcagctgttc cacggtcggc ttctgggtca tggtgttgac gaacttgttg gagaccacga acaggcgatc gatacggcct tcgaggtatg cgtcgagcat caccttgaca ctgccgatca ggtcgttgat cgaaggctct tcgccgaggt ggctgatagc tgccacgacg ttgccgccga aactgcggaa gaacgaagcg cccttgctac cgatcacgca aaggtcgatt tccgcccctt gctcgcggta gccgctcatg tccttgacga gggacttgaa caggttgatg ttcagaccgc cgcacagacc acggtcactg ctcaccacga tgtagccgac gcgcttgact tcacgctcga ccatgaacgg gtgacggtat tccgggttgg cgttggccag atggccgatc acctggcgaa tacgctccgc gtagggacgg ccggccgcca tgcgcatttg tgccttgcgc atcttgctca ccgccacttt ttccatggca ttggtgatct tttgcgtgct tttgatgctc gcaatcttgc tgcgaatctc ttttgcgcct gccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gcgcaaaaga gattcgcagc aagattgcga gcatcaaaag cacgcaaaag atcaccaatg ccatggaaaa agtggcggtg agcaagatgc gcaaggcaca aatgcgcatg gcggccggcc gtccctacgc ggagcgtatt cgccaggtga tcggccatct ggccaacgcc aacccggaat accgtcaccc gttcatggtc gagcgtgaag tcaagcgcgt cggctacatc gtggtgagca gtgaccgtgg tctgtgcggc ggtctgaaca tcaacctgtt caagtccctc gtcaaggaca tgagcggcta ccgcgagcaa ggggcggaaa tcgacctttg cgtgatcggt agcaagggcg cttcgttctt ccgcagtttc ggcggcaacg tcgtggcagc tatcagccac ctcggcgaag agccttcgat caacgacctg atcggcagtg tcaaggtgat gctcgacgca tacctcgaag gccgtatcga tcgcctgttc gtggtctcca acaagttcgt caacaccatg acccagaagc cgaccgtgga acagctgatt ccgctggtgg ccgatgacga tcaggagctg aagcaccact gggactatct ctacgaaccc gacgccaagt cgctcctcga cgggctgctg gtgcgttacg tggaatccca ggtgtaccag gccgtggttg agaacaacgc ctgtgagcag gcggcccgga tgattgcaat gaagaacgct accgacaacg ccggcgagct gatcagcgat ttgcagctga tctacaacaa ggcgcgtcag gcggcgatca cccaggaaat ctcggaaatc gtcggcggcg ctgccgcggt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat