CENTER ID: | PsaeA.17987.a |
ORGANISM: | Pseudomonas aeruginosa PAO1 |
ASSOCIATED DISEASE: | Pseudomonas infection |
CURRENT STATUS: | in PDB |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | unclassified |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
unclassified |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
PsaeA.17987.a.A1.GE41297 | full length | 1 | 367 | ||
PsaeA.17987.a.B1.GE38515 | full length | 1 | 367 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
PsaeA.17987.a.EN11.PD38369 | full length | 1 | 367 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|208964.12.peg.2 |
PGAT: | pseudomonas:PA0002 |
RefSeq: | NP_064722.1 |
UniProt: | Q9I7C4 |
HARVESTED ON: | data unavailable |
SEQUENCED ON: | 3/9/2017 |
EXPECTED MW: | 43kDa |
OBSERVED MW: | 24kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | data unavailable |
SEQUENCED ON: | 11/24/2014 |
EXPECTED MW: | 42kDa |
OBSERVED MW: | 105kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 97 |
PURIFICATION DATe: | 5/5/2019 |
CONCENTRATION: | 20.4mg/ml |
OBSERVED MW: | 52kDa |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol |
EXPRESSION HOST: | BL21 DE3 |
VIAL COUNT (approx.): | 66 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |