PsaeA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | PsaeA.17987.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17987.a.A1.GE41297 | full length | 1 | 367 | ||
| PsaeA.17987.a.B1.GE38515 | full length | 1 | 367 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17987.a.EN11.PD38369 | full length | 1 | 367 |
Structures
6PTH
DEPOSITED: 7/15/2019
DETERMINATION: XRay
CLONE: PsaeA.17987.a.EN11.GE43117
PROTEIN: PsaeA.17987.a.EN11.PD38369
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Commun Biol - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|208964.12.peg.2 |
| PGAT: | pseudomonas:PA0002 |
| RefSeq: | NP_064722.1 |
| UniProt: | Q9I7C4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHFTIQREAL LKPLQLVAGV VERRQTLPVL SNVLLVVEGQ QLSLTGTDLE VELVGRVVLE DAAEPGEITV PARKLMDICK SLPNDVLIDI RVEEQKLLVK AGRSRFTLST LPANDFPTVE EGPGSLNFSI AQSKLRRLID RTSFAMAQQD VRYYLNGMLL EVNGGTLRSV ATDGHRLAMC SLDAQIPSQD RHQVIVPRKG ILELARLLTE QDGEVGIVLG QHHIRATTGE FTFTSKLVDG KFPDYERVLP RGGDKLVVGD RQQLREAFSR TAILSNEKYR GIRLQLSNGL LKIQANNPEQ EEAEEEVQVE YNGGNLEIGF NVSYLLDVLG VIGTEQVRFI LSDSNSSALV HEADNDDSAY VVMPMRL
NT Sequence
atgcatttca ccattcaacg cgaagccctg ttgaaaccgc tgcaactggt cgccggcgtc gtggaacgcc gccagacatt gccggttctc tccaacgtcc tgctggtggt cgaaggccag caactgtcgc tgaccggcac cgacctcgaa gtcgagctgg ttggtcgcgt ggtactggaa gatgccgccg aacccggcga gatcaccgta ccggcgcgca agctgatgga catctgcaag agcctgccga acgacgtgct gatcgacatc cgtgtcgaag agcagaaact tctggtgaag gccgggcgta gccgcttcac cctgtccacc ctgccggcca acgatttccc caccgtagag gaaggtcccg gctcgctgaa cttcagcatt gcccagagca agctgcgtcg cctgatcgac cgcaccagct tcgccatggc ccagcaggac gtgcgttact acctcaacgg catgctgctg gaagtgaacg gcggcaccct gcgctccgtc gccaccgacg gccaccgact ggccatgtgc tcgctggatg cgcagatccc gtcgcaggac cgccaccagg tgatcgtgcc gcgcaaaggc atcctcgaac tggctcgtct gctcaccgag caggacggcg aagtcggcat cgtcctgggc cagcaccata tccgtgccac cactggcgaa ttcaccttca cttcgaagct ggtggacggc aagttcccgg actacgagcg tgtactgccg cgcggtggcg acaagctggt ggtcggtgac cgccagcaac tgcgcgaagc cttcagccgt accgcgatcc tctccaacga gaagtaccgc ggcattcgcc tgcagctttc caacggtttg ctgaaaatcc aggcgaacaa cccggagcag gaagaggccg aggaagaagt gcaggtcgag tacaacggcg gcaacctgga gataggcttc aacgtcagtt acctgctcga cgtgctgggt gtgatcggta ccgagcaggt ccgcttcatc ctttccgatt ccaacagcag cgccctggtc cacgaggccg acaatgacga ttctgcctat gtcgtcatgc cgatgcgcct ctaa
Details for PsaeA.17987.a.A1.GE41297
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMHFTIQREA LLKPLQLVAG VVERRQTLPV LSNVLLVVEG QQLSLTGTDL EVELVGRVVL EDAAEPGEIT VPARKLMDIC KSLPNDVLID IRVEEQKLLV KAGRSRFTLS TLPANDFPTV EEGPGSLNFS IAQSKLRRLI DRTSFAMAQQ DVRYYLNGML LEVNGGTLRS VATDGHRLAM CSLDAQIPSQ DRHQVIVPRK GILELARLLT EQDGEVGIVL GQHHIRATTG EFTFTSKLVD GKFPDYERVL PRGGDKLVVG DRQQLREAFS RTAILSNEKY RGIRLQLSNG LLKIQANNPE QEEAEEEVQV EYNGGNLEIG FNVSYLLDVL GVIGTEQVRF ILSDSNSSAL VHEADNDDSA YVVMPMRL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcatt tcaccattca acgcgaagcc ctgttgaaac cgctgcaact ggtcgccggc gtcgtggaac gccgccagac attgccggtt ctctccaacg tcctgctggt ggtcgaaggc cagcaactgt cgctgaccgg caccgacctc gaagtcgagc tggttggtcg cgtggtactg gaagatgccg ccgaacccgg cgagatcacc gtaccggcgc gcaagctgat ggacatctgc aagagcctgc cgaacgacgt gctgatcgac atccgtgtcg aagagcagaa acttctggtg aaggccgggc gtagccgctt caccctgtcc accctgccgg ccaacgattt ccccaccgta gaggaaggtc ccggctcgct gaacttcagc attgcccaga gcaagctgcg tcgcctgatc gaccgcacca gcttcgccat ggcccagcag gacgtgcgtt actacctcaa cggcatgctg ctggaagtga acggcggcac cctgcgctcc gtcgccaccg acggccaccg actggccatg tgctcgctgg atgcgcagat cccgtcgcag gaccgccacc aggtgatcgt gccgcgcaaa ggcatcctcg aactggctcg tctgctcacc gagcaggacg gcgaagtcgg catcgtcctg ggccagcacc atatccgtgc caccactggc gaattcacct tcacttcgaa gctggtggac ggcaagttcc cggactacga gcgtgtactg ccgcgcggtg gcgacaagct ggtggtcggt gaccgccagc aactgcgcga agccttcagc cgtaccgcga tcctctccaa cgagaagtac cgcggcattc gcctgcagct ttccaacggt ttgctgaaaa tccaggcgaa caacccggag caggaagagg ccgaggaaga agtgcaggtc gagtacaacg gcggcaacct ggagataggc ttcaacgtca gttacctgct cgacgtgctg ggtgtgatcg gtaccgagca ggtccgcttc atcctttccg attccaacag cagcgccctg gtccacgagg ccgacaatga cgattctgcc tatgtcgtca tgccgatgcg cctctaat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHFTIQREA LLKPLQLVAG VVERRQTLPV LSNVLLVVEG QQLSLTGTDL EVELVGRVVL EDAAEPGEIT VPARKLMDIC KSLPNDVLID IRVEEQKLLV KAGRSRFTLS TLPANDFPTV EEGPGSLNFS IAQSKLRRLI DRTSFAMAQQ DVRYYLNGML LEVNGGTLRS VATDGHRLAM CSLDAQIPSQ DRHQVIVPRK GILELARLLT EQDGEVGIVL GQHHIRATTG EFTFTSKLVD GKFPDYERVL PRGGDKLVVG DRQQLREAFS RTAILSNEKY RGIRLQLSNG LLKIQANNPE QEEAEEEVQV EYNGGNLEIG FNVSYLLDVL GVIGTEQVRF ILSDSNSSAL VHEADNDDSA YVVMPMRL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatttcacc attcaacgcg aagccctgtt gaaaccgctg caactggtcg ccggcgtcgt ggaacgccgc cagacattgc cggttctctc caacgtcctg ctggtggtcg aaggccagca actgtcgctg accggcaccg acctcgaagt cgagctggtt ggtcgcgtgg tactggaaga tgccgccgaa cccggcgaga tcaccgtacc ggcgcgcaag ctgatggaca tctgcaagag cctgccgaac gacgtgctga tcgacatccg tgtcgaagag cagaaacttc tggtgaaggc cgggcgtagc cgcttcaccc tgtccaccct gccggccaac gatttcccca ccgtagagga aggtcccggc tcgctgaact tcagcattgc ccagagcaag ctgcgtcgcc tgatcgaccg caccagcttc gccatggccc agcaggacgt gcgttactac ctcaacggca tgctgctgga agtgaacggc ggcaccctgc gctccgtcgc caccgacggc caccgactgg ccatgtgctc gctggatgcg cagatcccgt cgcaggaccg ccaccaggtg atcgtgccgc gcaaaggcat cctcgaactg gctcgtctgc tcaccgagca ggacggcgaa gtcggcatcg tcctgggcca gcaccatatc cgtgccacca ctggcgaatt caccttcact tcgaagctgg tggacggcaa gttcccggac tacgagcgtg tactgccgcg cggtggcgac aagctggtgg tcggtgaccg ccagcaactg cgcgaagcct tcagccgtac cgcgatcctc tccaacgaga agtaccgcgg cattcgcctg cagctttcca acggtttgct gaaaatccag gcgaacaacc cggagcagga agaggccgag gaagaagtgc aggtcgagta caacggcggc aacctggaga taggcttcaa cgtcagttac ctgctcgacg tgctgggtgt gatcggtacc gagcaggtcc gcttcatcct ttccgattcc aacagcagcg ccctggtcca cgaggccgac aatgacgatt ctgcctatgt cgtcatgccg atgcgcctct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for PsaeA.17987.a.B1.GE38515
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 11/24/2014 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 105kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMH FTIQREALLK PLQLVAGVVE RRQTLPVLSN VLLVVEGQQL SLTGTDLEVE LVGRVVLEDA AEPGEITVPA RKLMDICKSL PNDVLIDIRV EEQKLLVKAG RSRFTLSTLP ANDFPTVEEG PGSLNFSIAQ SKLRRLIDRT SFAMAQQDVR YYLNGMLLEV NGGTLRSVAT DGHRLAMCSL DAQIPSQDRH QVIVPRKGIL ELARLLTEQD GEVGIVLGQH HIRATTGEFT FTSKLVDGKF PDYERVLPRG GDKLVVGDRQ QLREAFSRTA ILSNEKYRGI RLQLSNGLLK IQANNPEQEE AEEEVQVEYN GGNLEIGFNV SYLLDVLGVI GTEQVRFILS DSNSSALVHE ADNDDS
Validated NT Sequence
ggcagaatcg tcattgtcgg cctcgtggac cagggcgctg ctgttggaat cggaaaggat gaagcggacc tgctcggtac cgatcacacc cagcacgtcg agcaggtaac tgacgttgaa gcctatctcc aggttgccgc cgttgtactc gacctgcact tcttcctcgg cctcttcctg ctccgggttg ttcgcctgga ttttcagcaa accgttggaa agctgcaggc gaatgccgcg gtacttctcg ttggagagga tcgcggtacg gctgaaggct tcgcgcagtt gctggcggtc accgaccacc agcttgtcgc caccgcgcgg cagtacacgc tcgtagtccg ggaacttgcc gtccaccagc ttcgaagtga aggtgaattc gccagtggtg gcacggatat ggtgctggcc caggacgatg ccgacttcgc cgtcctgctc ggtgagcaga cgagccagtt cgaggatgcc tttgcgcggc acgatcacct ggtggcggtc ctgcgacggg atctgcgcat ccagcgagca catggccagt cggtggccgt cggtggcgac ggagcgcagg gtgccgccgt tcacttccag cagcatgccg ttgaggtagt aacgcacgtc ctgctgggcc atggcgaagc tggtgcggtc gatcaggcga cgcagcttgc tctgggcaat gctgaagttc agcgagccgg gaccttcctc tacggtgggg aaatcgttgg ccggcagggt ggacagggtg aagcggctac gcccggcctt caccagaagt ttctgctctt cgacacggat gtcgatcagc acgtcgttcg gcaggctctt gcagatgtcc atcagcttgc gcgccggtac ggtgatctcg ccgggttcgg cggcatcttc cagtaccacg cgaccaacca gctcgacttc gaggtcggtg ccggtcagcg acagttgctg gccttcgacc accagcagga cgttggagag aaccggcaat gtctggcggc gttccacgac gccggcgacc agttgcagcg gtttcaacag ggcttcgcgt tgaatggtga aatgcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMH FTIQREALLK PLQLVAGVVE RRQTLPVLSN VLLVVEGQQL SLTGTDLEVE LVGRVVLEDA AEPGEITVPA RKLMDICKSL PNDVLIDIRV EEQKLLVKAG RSRFTLSTLP ANDFPTVEEG PGSLNFSIAQ SKLRRLIDRT SFAMAQQDVR YYLNGMLLEV NGGTLRSVAT DGHRLAMCSL DAQIPSQDRH QVIVPRKGIL ELARLLTEQD GEVGIVLGQH HIRATTGEFT FTSKLVDGKF PDYERVLPRG GDKLVVGDRQ QLREAFSRTA ILSNEKYRGI RLQLSNGLLK IQANNPEQEE AEEEVQVEYN GGNLEIGFNV SYLLDVLGVI GTEQVRFILS DSNSSALVHE ADNDDSAYVV MPMRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatt tcaccattca acgcgaagcc ctgttgaaac cgctgcaact ggtcgccggc gtcgtggaac gccgccagac attgccggtt ctctccaacg tcctgctggt ggtcgaaggc cagcaactgt cgctgaccgg caccgacctc gaagtcgagc tggttggtcg cgtggtactg gaagatgccg ccgaacccgg cgagatcacc gtaccggcgc gcaagctgat ggacatctgc aagagcctgc cgaacgacgt gctgatcgac atccgtgtcg aagagcagaa acttctggtg aaggccgggc gtagccgctt caccctgtcc accctgccgg ccaacgattt ccccaccgta gaggaaggtc ccggctcgct gaacttcagc attgcccaga gcaagctgcg tcgcctgatc gaccgcacca gcttcgccat ggcccagcag gacgtgcgtt actacctcaa cggcatgctg ctggaagtga acggcggcac cctgcgctcc gtcgccaccg acggccaccg actggccatg tgctcgctgg atgcgcagat cccgtcgcag gaccgccacc aggtgatcgt gccgcgcaaa ggcatcctcg aactggctcg tctgctcacc gagcaggacg gcgaagtcgg catcgtcctg ggccagcacc atatccgtgc caccactggc gaattcacct tcacttcgaa gctggtggac ggcaagttcc cggactacga gcgtgtactg ccgcgcggtg gcgacaagct ggtggtcggt gaccgccagc aactgcgcga agccttcagc cgtaccgcga tcctctccaa cgagaagtac cgcggcattc gcctgcagct ttccaacggt ttgctgaaaa tccaggcgaa caacccggag caggaagagg ccgaggaaga agtgcaggtc gagtacaacg gcggcaacct ggagataggc ttcaacgtca gttacctgct cgacgtgctg ggtgtgatcg gtaccgagca ggtccgcttc atcctttccg attccaacag cagcgccctg gtccacgagg ccgacaatga cgattctgcc tatgtcgtca tgccgatgcg cctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for PsaeA.17987.a.EN11.PD38369
| PURIFICATION DATe: | 5/5/2019 |
| CONCENTRATION: | 20.4mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25mM Tris pH 8.0, 200mM NaCl, 1mM TCEP, 1% Glycerol |
| EXPRESSION HOST: | BL21 DE3 |
| VIAL COUNT (approx.): | 66 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMHFT IQREALLKPL QLVAGVVERR QTLPVLSNVL LVVEGQQLSL TGTDLEVELV GRVVLEDAAE PGEITVPARK LMDICKSLPN DVLIDIRVEE QKLLVKAGRS RFTLSTLPAN DFPTVEEGPG SLNFSIAQSK LRRLIDRTSF AMAQQDVRYY LNGMLLEVNG GTLRSVATDG HRLAMCSLDA QIPSQDRHQV IVPRKGILEL ARLLTEQDGE VGIVLGQHHI RATTGEFTFT SKLVDGKFPD YERVLPRGGD KLVVGDRQQL REAFSRTAIL SNEKYRGIRL QLSNGLLKIQ ANNPEQEEAE EEVQVEYNGG NLEIGFNVSY LLDVLGVIGT EQVRFILSDS NSSALVHEAD NDDSAYVVMP MRL
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GCACTTTACC ATCCAGCGTG AAGCACTGCT GAAACCGCTG CAGCTGGTAG CAGGCGTAGT TGAACGCCGT CAAACTCTGC CGGTTCTGTC CAACGTTCTG CTGGTTGTTG AAGGCCAACA GTTGAGCTTA ACTGGCACCG ACCTGGAAGT TGAGCTGGTA GGTCGTGTGG TTCTGGAAGA CGCAGCGGAA CCGGGCGAAA TTACTGTTCC GGCTCGTAAG CTCATGGACA TCTGCAAATC CCTGCCGAAC GACGTTCTGA TCGATATCCG TGTGGAAGAA CAGAAGCTGC TGGTAAAAGC GGGTCGTAGC CGCTTTACCC TGTCCACTCT GCCGGCAAAC GACTTCCCGA CCGTTGAAGA AGGCCCGGGT AGCCTGAATT TCAGCATCGC CCAGTCTAAA CTGCGTCGCC TCATCGACCG TACCTCTTTC GCTATGGCTC AACAGGATGT TCGTTACTAT CTGAACGGTA TGCTTCTGGA AGTTAACGGC GGCACCCTGC GTAGCGTAGC TACTGACGGC CACCGCCTGG CTATGTGTTC ACTGGACGCC CAAATCCCGT CCCAAGATCG CCACCAAGTC ATCGTTCCGC GCAAAGGTAT TCTCGAACTG GCACGTCTGC TGACCGAGCA GGATGGTGAG GTTGGTATCG TTCTGGGCCA GCACCACATC CGTGCAACCA CCGGCGAATT TACTTTTACG AGCAAACTGG TCGACGGCAA GTTTCCAGAC TACGAACGTG TGCTGCCGCG TGGTGGTGAC AAACTGGTTG TAGGTGATCG TCAGCAGCTC AGAGAAGCAT TCTCCCGTAC CGCTATCCTG TCGAACGAGA AGTACCGTGG TATCCGTTTA CAGCTGTCGA ATGGTCTGCT GAAAATCCAG GCGAACAACC CGGAACAAGA AGAAGCTGAG GAAGAGGTTC AGGTAGAATA CAACGGTGGC AACCTGGAAA TCGGCTTCAA CGTTTCCTAC CTGCTGGATG TGCTGGGCGT TATCGGCACC GAACAGGTAC GTTTCATCCT GTCCGACTCT AACAGCTCTG CACTGGTACA CGAGGCTGAC AACGATGACA GCGCATACGT GGTGATGCCG ATGCGTTTGT GATAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T