PsaeA.17981.a
Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase) (ProRS)
| CENTER ID: | PsaeA.17981.a |
| ORGANISM: | Pseudomonas aeruginosa PAO1 |
| ASSOCIATED DISEASE: | Pseudomonas infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17981.a.B1.GE38513 | full length | 1 | 571 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| PsaeA.17981.a.B1.PW37621 | full length | 1 | 571 |
Structures
5UCM
DEPOSITED: 12/22/2016
DETERMINATION: XRay
CLONE: PsaeA.17981.a.B1.GE38513
PROTEIN: PsaeA.17981.a.B1.PW37621
Publications by SSGCID
Characterization and structure determination of prolyl-tRNA synthetase from Pseudomonas aeruginosa and development as a screening platform
Dranow DM, Bullard JM, Pena N, Hu Y, Escamilla Y
Protein Sci. - 2019
PMID: 30666738 ; PMCID: PMC6423717
PDB:
5UCM
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|208964.12.peg.994 |
| PGAT: | pseudomonas:PA0956 |
| RefSeq: | NP_249647.1 |
| UniProt: | Q9I502 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTSQYLLST LKETPADAVV ISHQLLLRAG MIRRLASGLY TWLPMGLRVL RKVETIVREE MNAAGALEVL MPAVQPAELW QESGRWEQYG PELLRLKDRH EREFCVGPTH EEVITDLARN ELNSYKQLPI NFYQIQTKFR DEIRPRFGLM RGREFIMKDA YSFHLSQDSL QQTYDGMYQA YSKIFSRLGL DFRPVQADNG SIGGSGSHEF HVLANSGEDD IVFSDSSDYA ANIEKAEAVP RESARGSATE DMRLVDTPNT KTIAALVDGF QLPIEKTIKT LVVHGAEEGT LVALIVRGDH ELNEIKAANQ PLVASPLVFA SEAEIRAAIG AGPGSLGPVN LPIACIVDRS VALMSDFAAG ANIEDKHYFG VNWERDLPLP EVADLRNVVE GDPSPDGKGT LVIKRGIEVG HIFQLGTKYS EAMKLSVLSE QGKPVNLIMG CYGIGVSRVV AAAIEQNHDE RGILWPSALA PFQIALVPLK YETESVKQAT DKLYAELTAA GFEVLLDDRD KKTSPGVKFA DMELIGIPHR IVISDRGLSE GVLEYKGRRD SESQNLPIGE LMSFITEKLS R
NT Sequence
atgcgtacca gtcagtatct gctttccacg ctcaaagaaa cccccgccga cgcggtggta atcagccatc aactgctgct tcgcgccggc atgatccgtc gcctggcctc gggtctctac acctggctgc caatgggcct gcgggtactg cgcaaggtcg agaccatcgt ccgcgaggaa atgaacgctg ccggcgccct ggaagtgctg atgccggcgg tgcagccggc cgagctgtgg caggagtcgg ggcgctggga gcaatacggc cctgagctgc tgcgcctgaa ggaccgccac gagcgcgaat tctgcgtagg cccgacccac gaagaagtga tcaccgacct cgcgcgcaac gagctgaaca gctacaagca actgccgatc aacttctatc agatccagac caagttccgc gatgagatcc gcccgcgctt cggcctgatg cgcggacgcg aattcatcat gaaggacgcc tattccttcc acctgagcca ggactcgctg cagcagacct acgacgggat gtaccaggcg tacagcaaga tcttctcccg cctcggcctg gatttccgtc ccgtgcaagc cgacaacggc tccatcggcg gcagcggctc ccatgagttc cacgtgctgg ccaactccgg cgaggacgat atcgtcttca gcgacagctc cgactacgcc gcgaacatcg agaaggccga agccgtgccg cgcgagagcg cccgcggcag cgccaccgag gacatgcgcc tggtcgacac gcccaacacc aagaccatcg cggccctggt ggacggcttc cagctaccga tcgagaaaac catcaagacc ctggtggtgc atggcgccga agaaggcacc ctggtcgccc tgatcgtccg cggtgatcac gagctcaacg aaatcaaggc cgccaaccag ccgctggtcg ccagcccgct ggtattcgct tcggaggccg agatccgtgc cgccatcggt gccggccccg gctcgctggg cccggtcaac ctgccgatcg cctgcatcgt cgaccgctcc gtcgccctga tgagcgattt cgccgctggc gccaacatcg aggacaagca ctacttcggc gtgaactggg agcgcgacct gccgctgccc gaggtcgccg acctgcgcaa tgtcgtcgaa ggcgacccca gcccggacgg caagggcacc ctggtgatca agcgcggcat cgaggtcggt catatcttcc agcttggcac caagtacagc gaagcgatga agctgagcgt cctgagcgaa cagggcaagc cggtcaacct gatcatgggc tgctacggca tcggcgtatc ccgcgtggtc gccgccgcca tcgagcagaa ccatgacgag cgcggcattc tctggccaag cgcattggcg ccgttccaga tcgccctggt accgctgaag tacgaaaccg agagcgtcaa gcaggcgacc gacaagctct atgccgagct gaccgcagcc ggtttcgaag tgctgctgga cgatcgcgac aagaagacca gccccggggt gaagttcgcc gacatggagc tgatcggcat tccccaccgc atcgtgatca gcgaccgcgg cctcagcgaa ggcgtcctgg aatacaaggg ccgccgcgac agcgagtcgc agaacctgcc gatcggcgaa ctgatgtcgt tcatcaccga aaaactcagc cgctga
Details for PsaeA.17981.a.B1.GE38513
| HARVESTED ON: | 10/9/2014 |
| SEQUENCED ON: | 11/24/2014 |
| EXPECTED MW: | 64kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR TSQYLLSTLK ETPADAVVIS HQLLLRAGMI RRLASGLYTW LPMGLRVLRK VETIVREEMN AAGALEVLMP AVQPAELWQE SGRWEQYGPE LLRLKDRHER EFCVGPTHEE VITDLARNEL NSYKQLPINF YQIQTKFRDE IRPRFGLMRG REFIMKDAYS FHLSQDSLQQ TYDGMYQAYS KIFSRLGLDF RPVQADNGSI GGSGSHEFHV LANSGEDDIV FSDSSDYAAN IEKAEAVPRE SARGSATEDM RLVDTPNTKT IAALVDGFQL PIEKTIKTLV VHGAEEGTLV ALIVRGDHEL NEIKAANQPL VASPLVFASE AEIRAAIGAG PGSLGPVNLP IACIVDRSVA LMSDFAAGAN IEDKHYFGVN WERDLPLPEV ADLRNVVEGD PSPDGKGTLV IKRGIEVGHI FQLGTKYSEA MKLSVLSEQG KPVNLIMGCY GIGVSRVVAA AIEQNHDERG ILWPSALAPF QIALVPLKYE TESVKQATDK LYAELTAAGF EVLLDDRDKK TSPGVKFADM ELIGIPHRIV ISDRGLSEGV LEYKGRRDSE SQNLPIGELM SFITEKLSR
Validated NT Sequence
atggctcacc accaccacca ccatatgcgt accagtcagt atctgctttc cacgctcaaa gaaacccccg ccgacgcggt ggtaatcagc catcaactgc tgcttcgcgc cggcatgatc cgtcgcctgg cctcgggtct ctacacctgg ctgccaatgg gcctgcgggt actgcgcaag gtcgagacca tcgtccgcga ggaaatgaac gctgccggcg ccctggaagt gctgatgccg gcggtgcagc cggccgagct gtggcaggag tcggggcgct gggagcaata cggccctgag ctgctgcgcc tgaaggaccg ccacgagcgc gaattctgcg taggcccgac ccacgaagaa gtgatcaccg acctcgcgcg caacgagctg aacagctaca agcaactgcc gatcaacttc tatcagatcc agaccaagtt ccgcgatgag atccgcccgc gcttcggcct gatgcgcgga cgcgaattca tcatgaagga cgcctattcc ttccacctga gccaggactc gctgcagcag acctacgacg ggatgtacca ggcgtacagc aagatcttct cccgcctcgg cctggatttc cgtcccgtgc aagccgacaa cggctccatc ggcggcagcg gctcccatga gttccacgtg ctggccaact ccggcgagga cgatatcgtc ttcagcgaca gctccgacta cgccgcgaac atcgagaagg ccgaagccgt gccgcgcgag agcgcccgcg gcagcgccac cgaggacatg cgcctggtcg acacgcccaa caccaagacc atcgcggccc tggtggacgg cttccagcta ccgatcgaga aaaccatcaa gaccctggtg gtgcatggcg ccgaagaagg caccctggtc gccctgatcg tccgcggtga tcacgagctc aacgaaatca aggccgccaa ccagccgctg gtcgccagcc cgctggtatt cgcttcggag gccgagatcc gtgccgccat cggtgccggc cccggctcgc tgggcccggt caacctgccg atcgcctgca tcgtcgaccg ctccgtcgcc ctgatgagcg atttcgccgc tggcgccaac atcgaggaca agcactactt cggcgtgaac tgggagcgcg acctgccgct gcccgaggtc gccgacctgc gcaatgtcgt cgaaggcgac cccagcccgg acggcaaggg caccctggtg atcaagcgcg gcatcgaggt cggtcatatc ttccagcttg gcaccaagta cagcgaagcg atgaagctga gcgtcctgag cgaacagggc aagccggtca acctgatcat gggctgctac ggcatcggcg tatcccgcgt ggtcgccgcc gccatcgagc agaaccatga cgagcgcggc attctctggc caagcgcatt ggcgccgttc cagatcgccc tggtaccgct gaagtacgaa accgagagcg tcaagcaggc gaccgacaag ctctatgccg agctgaccgc agccggtttc gaagtgctgc tggacgatcg cgacaagaag accagccccg gggtgaagtt cgccgacatg gagctgatcg gcattcccca ccgcatcgtg atcagcgacc gcggcctcag cgaaggcgtc ctggaataca agggccgccg cgacagcgag tcgcagaacc tgccgatcgg cgaactgatg tcgttcatca ccgaaaaact cagccgctaa
Expected Protein Sequence
MAHHHHHHMR TSQYLLSTLK ETPADAVVIS HQLLLRAGMI RRLASGLYTW LPMGLRVLRK VETIVREEMN AAGALEVLMP AVQPAELWQE SGRWEQYGPE LLRLKDRHER EFCVGPTHEE VITDLARNEL NSYKQLPINF YQIQTKFRDE IRPRFGLMRG REFIMKDAYS FHLSQDSLQQ TYDGMYQAYS KIFSRLGLDF RPVQADNGSI GGSGSHEFHV LANSGEDDIV FSDSSDYAAN IEKAEAVPRE SARGSATEDM RLVDTPNTKT IAALVDGFQL PIEKTIKTLV VHGAEEGTLV ALIVRGDHEL NEIKAANQPL VASPLVFASE AEIRAAIGAG PGSLGPVNLP IACIVDRSVA LMSDFAAGAN IEDKHYFGVN WERDLPLPEV ADLRNVVEGD PSPDGKGTLV IKRGIEVGHI FQLGTKYSEA MKLSVLSEQG KPVNLIMGCY GIGVSRVVAA AIEQNHDERG ILWPSALAPF QIALVPLKYE TESVKQATDK LYAELTAAGF EVLLDDRDKK TSPGVKFADM ELIGIPHRIV ISDRGLSEGV LEYKGRRDSE SQNLPIGELM SFITEKLSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta ccagtcagta tctgctttcc acgctcaaag aaacccccgc cgacgcggtg gtaatcagcc atcaactgct gcttcgcgcc ggcatgatcc gtcgcctggc ctcgggtctc tacacctggc tgccaatggg cctgcgggta ctgcgcaagg tcgagaccat cgtccgcgag gaaatgaacg ctgccggcgc cctggaagtg ctgatgccgg cggtgcagcc ggccgagctg tggcaggagt cggggcgctg ggagcaatac ggccctgagc tgctgcgcct gaaggaccgc cacgagcgcg aattctgcgt aggcccgacc cacgaagaag tgatcaccga cctcgcgcgc aacgagctga acagctacaa gcaactgccg atcaacttct atcagatcca gaccaagttc cgcgatgaga tccgcccgcg cttcggcctg atgcgcggac gcgaattcat catgaaggac gcctattcct tccacctgag ccaggactcg ctgcagcaga cctacgacgg gatgtaccag gcgtacagca agatcttctc ccgcctcggc ctggatttcc gtcccgtgca agccgacaac ggctccatcg gcggcagcgg ctcccatgag ttccacgtgc tggccaactc cggcgaggac gatatcgtct tcagcgacag ctccgactac gccgcgaaca tcgagaaggc cgaagccgtg ccgcgcgaga gcgcccgcgg cagcgccacc gaggacatgc gcctggtcga cacgcccaac accaagacca tcgcggccct ggtggacggc ttccagctac cgatcgagaa aaccatcaag accctggtgg tgcatggcgc cgaagaaggc accctggtcg ccctgatcgt ccgcggtgat cacgagctca acgaaatcaa ggccgccaac cagccgctgg tcgccagccc gctggtattc gcttcggagg ccgagatccg tgccgccatc ggtgccggcc ccggctcgct gggcccggtc aacctgccga tcgcctgcat cgtcgaccgc tccgtcgccc tgatgagcga tttcgccgct ggcgccaaca tcgaggacaa gcactacttc ggcgtgaact gggagcgcga cctgccgctg cccgaggtcg ccgacctgcg caatgtcgtc gaaggcgacc ccagcccgga cggcaagggc accctggtga tcaagcgcgg catcgaggtc ggtcatatct tccagcttgg caccaagtac agcgaagcga tgaagctgag cgtcctgagc gaacagggca agccggtcaa cctgatcatg ggctgctacg gcatcggcgt atcccgcgtg gtcgccgccg ccatcgagca gaaccatgac gagcgcggca ttctctggcc aagcgcattg gcgccgttcc agatcgccct ggtaccgctg aagtacgaaa ccgagagcgt caagcaggcg accgacaagc tctatgccga gctgaccgca gccggtttcg aagtgctgct ggacgatcgc gacaagaaga ccagccccgg ggtgaagttc gccgacatgg agctgatcgg cattccccac cgcatcgtga tcagcgaccg cggcctcagc gaaggcgtcc tggaatacaa gggccgccgc gacagcgagt cgcagaacct gccgatcggc gaactgatgt cgttcatcac cgaaaaactc agccgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for PsaeA.17981.a.B1.PW37621
| PURIFICATION DATe: | 1/5/2015 |
| CONCENTRATION: | 9.5mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR TSQYLLSTLK ETPADAVVIS HQLLLRAGMI RRLASGLYTW LPMGLRVLRK VETIVREEMN AAGALEVLMP AVQPAELWQE SGRWEQYGPE LLRLKDRHER EFCVGPTHEE VITDLARNEL NSYKQLPINF YQIQTKFRDE IRPRFGLMRG REFIMKDAYS FHLSQDSLQQ TYDGMYQAYS KIFSRLGLDF RPVQADNGSI GGSGSHEFHV LANSGEDDIV FSDSSDYAAN IEKAEAVPRE SARGSATEDM RLVDTPNTKT IAALVDGFQL PIEKTIKTLV VHGAEEGTLV ALIVRGDHEL NEIKAANQPL VASPLVFASE AEIRAAIGAG PGSLGPVNLP IACIVDRSVA LMSDFAAGAN IEDKHYFGVN WERDLPLPEV ADLRNVVEGD PSPDGKGTLV IKRGIEVGHI FQLGTKYSEA MKLSVLSEQG KPVNLIMGCY GIGVSRVVAA AIEQNHDERG ILWPSALAPF QIALVPLKYE TESVKQATDK LYAELTAAGF EVLLDDRDKK TSPGVKFADM ELIGIPHRIV ISDRGLSEGV LEYKGRRDSE SQNLPIGELM SFITEKLSR
Validated NT Sequence
atggctcacc accaccacca ccatatgcgt accagtcagt atctgctttc cacgctcaaa gaaacccccg ccgacgcggt ggtaatcagc catcaactgc tgcttcgcgc cggcatgatc cgtcgcctgg cctcgggtct ctacacctgg ctgccaatgg gcctgcgggt actgcgcaag gtcgagacca tcgtccgcga ggaaatgaac gctgccggcg ccctggaagt gctgatgccg gcggtgcagc cggccgagct gtggcaggag tcggggcgct gggagcaata cggccctgag ctgctgcgcc tgaaggaccg ccacgagcgc gaattctgcg taggcccgac ccacgaagaa gtgatcaccg acctcgcgcg caacgagctg aacagctaca agcaactgcc gatcaacttc tatcagatcc agaccaagtt ccgcgatgag atccgcccgc gcttcggcct gatgcgcgga cgcgaattca tcatgaagga cgcctattcc ttccacctga gccaggactc gctgcagcag acctacgacg ggatgtacca ggcgtacagc aagatcttct cccgcctcgg cctggatttc cgtcccgtgc aagccgacaa cggctccatc ggcggcagcg gctcccatga gttccacgtg ctggccaact ccggcgagga cgatatcgtc ttcagcgaca gctccgacta cgccgcgaac atcgagaagg ccgaagccgt gccgcgcgag agcgcccgcg gcagcgccac cgaggacatg cgcctggtcg acacgcccaa caccaagacc atcgcggccc tggtggacgg cttccagcta ccgatcgaga aaaccatcaa gaccctggtg gtgcatggcg ccgaagaagg caccctggtc gccctgatcg tccgcggtga tcacgagctc aacgaaatca aggccgccaa ccagccgctg gtcgccagcc cgctggtatt cgcttcggag gccgagatcc gtgccgccat cggtgccggc cccggctcgc tgggcccggt caacctgccg atcgcctgca tcgtcgaccg ctccgtcgcc ctgatgagcg atttcgccgc tggcgccaac atcgaggaca agcactactt cggcgtgaac tgggagcgcg acctgccgct gcccgaggtc gccgacctgc gcaatgtcgt cgaaggcgac cccagcccgg acggcaaggg caccctggtg atcaagcgcg gcatcgaggt cggtcatatc ttccagcttg gcaccaagta cagcgaagcg atgaagctga gcgtcctgag cgaacagggc aagccggtca acctgatcat gggctgctac ggcatcggcg tatcccgcgt ggtcgccgcc gccatcgagc agaaccatga cgagcgcggc attctctggc caagcgcatt ggcgccgttc cagatcgccc tggtaccgct gaagtacgaa accgagagcg tcaagcaggc gaccgacaag ctctatgccg agctgaccgc agccggtttc gaagtgctgc tggacgatcg cgacaagaag accagccccg gggtgaagtt cgccgacatg gagctgatcg gcattcccca ccgcatcgtg atcagcgacc gcggcctcag cgaaggcgtc ctggaataca agggccgccg cgacagcgag tcgcagaacc tgccgatcgg cgaactgatg tcgttcatca ccgaaaaact cagccgctaa
Expressed Protein Sequence
MAHHHHHHMR TSQYLLSTLK ETPADAVVIS HQLLLRAGMI RRLASGLYTW LPMGLRVLRK VETIVREEMN AAGALEVLMP AVQPAELWQE SGRWEQYGPE LLRLKDRHER EFCVGPTHEE VITDLARNEL NSYKQLPINF YQIQTKFRDE IRPRFGLMRG REFIMKDAYS FHLSQDSLQQ TYDGMYQAYS KIFSRLGLDF RPVQADNGSI GGSGSHEFHV LANSGEDDIV FSDSSDYAAN IEKAEAVPRE SARGSATEDM RLVDTPNTKT IAALVDGFQL PIEKTIKTLV VHGAEEGTLV ALIVRGDHEL NEIKAANQPL VASPLVFASE AEIRAAIGAG PGSLGPVNLP IACIVDRSVA LMSDFAAGAN IEDKHYFGVN WERDLPLPEV ADLRNVVEGD PSPDGKGTLV IKRGIEVGHI FQLGTKYSEA MKLSVLSEQG KPVNLIMGCY GIGVSRVVAA AIEQNHDERG ILWPSALAPF QIALVPLKYE TESVKQATDK LYAELTAAGF EVLLDDRDKK TSPGVKFADM ELIGIPHRIV ISDRGLSEGV LEYKGRRDSE SQNLPIGELM SFITEKLSR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTA CCAGTCAGTA TCTGCTTTCC ACGCTCAAAG AAACCCCCGC CGACGCGGTG GTAATCAGCC ATCAACTGCT GCTTCGCGCC GGCATGATCC GTCGCCTGGC CTCGGGTCTC TACACCTGGC TGCCAATGGG CCTGCGGGTA CTGCGCAAGG TCGAGACCAT CGTCCGCGAG GAAATGAACG CTGCCGGCGC CCTGGAAGTG CTGATGCCGG CGGTGCAGCC GGCCGAGCTG TGGCAGGAGT CGGGGCGCTG GGAGCAATAC GGCCCTGAGC TGCTGCGCCT GAAGGACCGC CACGAGCGCG AATTCTGCGT AGGCCCGACC CACGAAGAAG TGATCACCGA CCTCGCGCGC AACGAGCTGA ACAGCTACAA GCAACTGCCG ATCAACTTCT ATCAGATCCA GACCAAGTTC CGCGATGAGA TCCGCCCGCG CTTCGGCCTG ATGCGCGGAC GCGAATTCAT CATGAAGGAC GCCTATTCCT TCCACCTGAG CCAGGACTCG CTGCAGCAGA CCTACGACGG GATGTACCAG GCGTACAGCA AGATCTTCTC CCGCCTCGGC CTGGATTTCC GTCCCGTGCA AGCCGACAAC GGCTCCATCG GCGGCAGCGG CTCCCATGAG TTCCACGTGC TGGCCAACTC CGGCGAGGAC GATATCGTCT TCAGCGACAG CTCCGACTAC GCCGCGAACA TCGAGAAGGC CGAAGCCGTG CCGCGCGAGA GCGCCCGCGG CAGCGCCACC GAGGACATGC GCCTGGTCGA CACGCCCAAC ACCAAGACCA TCGCGGCCCT GGTGGACGGC TTCCAGCTAC CGATCGAGAA AACCATCAAG ACCCTGGTGG TGCATGGCGC CGAAGAAGGC ACCCTGGTCG CCCTGATCGT CCGCGGTGAT CACGAGCTCA ACGAAATCAA GGCCGCCAAC CAGCCGCTGG TCGCCAGCCC GCTGGTATTC GCTTCGGAGG CCGAGATCCG TGCCGCCATC GGTGCCGGCC CCGGCTCGCT GGGCCCGGTC AACCTGCCGA TCGCCTGCAT CGTCGACCGC TCCGTCGCCC TGATGAGCGA TTTCGCCGCT GGCGCCAACA TCGAGGACAA GCACTACTTC GGCGTGAACT GGGAGCGCGA CCTGCCGCTG CCCGAGGTCG CCGACCTGCG CAATGTCGTC GAAGGCGACC CCAGCCCGGA CGGCAAGGGC ACCCTGGTGA TCAAGCGCGG CATCGAGGTC GGTCATATCT TCCAGCTTGG CACCAAGTAC AGCGAAGCGA TGAAGCTGAG CGTCCTGAGC GAACAGGGCA AGCCGGTCAA CCTGATCATG GGCTGCTACG GCATCGGCGT ATCCCGCGTG GTCGCCGCCG CCATCGAGCA GAACCATGAC GAGCGCGGCA TTCTCTGGCC AAGCGCATTG GCGCCGTTCC AGATCGCCCT GGTACCGCTG AAGTACGAAA CCGAGAGCGT CAAGCAGGCG ACCGACAAGC TCTATGCCGA GCTGACCGCA GCCGGTTTCG AAGTGCTGCT GGACGATCGC GACAAGAAGA CCAGCCCCGG GGTGAAGTTC GCCGACATGG AGCTGATCGG CATTCCCCAC CGCATCGTGA TCAGCGACCG CGGCCTCAGC GAAGGCGTCC TGGAATACAA GGGCCGCCGC GACAGCGAGT CGCAGAACCT GCCGATCGGC GAACTGATGT CGTTCATCAC CGAAAAACTC AGCCGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T