PsaeA.17976.a

30S ribosomal protein S5

CENTER ID: PsaeA.17976.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17976.a.B1.GE38510 full length 1 166
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4447
PGAT: pseudomonas:PA4246
RefSeq: NP_252936.1
UniProt: Q9HWF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANNEQKRDE GYIEKLVQVN RVAKTVKGGR IFAFTALTVV GDGKGRVGFG RGKAREVPAA IQKAMEAARR NMIQVDLNGT TLQYPTKSAH GASKVYMQPA SEGTGIIAGG AMRAVLEVAG VQNVLAKCYG STNPVNVVYA TFKGLKNMQA PEAVAAKRGK SVEEIL
NT Sequence
atggcaaaca acgagcaaaa gcgcgacgaa ggctacatcg agaagctggt tcaggttaac cgcgtcgcca aaaccgtaaa aggtggccgt atcttcgctt tcaccgcact gaccgtggtg ggtgatggca agggtcgcgt tggtttcggt cgtggcaagg cgcgtgaagt gccggccgcc atccagaaag caatggaagc cgctcgccgc aacatgatcc aagtcgatct gaacggcacc accctgcagt atccgaccaa gtccgcccat ggcgcctcca aggtgtacat gcagccggct tcggaaggta ccggcatcat cgccggcggc gccatgcgtg ccgttctgga agtggctggt gtgcagaacg tcctggccaa gtgctatggc tccaccaatc cggtgaacgt ggtttacgcc accttcaagg gcctgaagaa catgcaggct ccggaagcag tagcggccaa gcgtggcaag agcgtcgagg agattctcta a
Details for PsaeA.17976.a.B1.GE38510
HARVESTED ON: data unavailable
SEQUENCED ON: 11/24/2014
EXPECTED MW: 19kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHMAN NEQKRDEGYI EKLVQVNRVA KTVKGGRIFA FTALTVVGDG KGRVGFGRGK AREVPAAIQK AMEAARRNMI QVDLNGTTLQ YPTKSAHGAS KVYMQPASEG TGIIAGGAMR AVLEVAGVQN VLAKCYGSTN PVNVVYATFK GLKNMQAPEA VAAKRGKSVE KIL
Validated NT Sequence
atcttactca cttagagaat cttttcgacg ctcttgccac gcttggccgc tactgcttcc ggagcctgca tgttcttcag gcccttgaag gtggcgtaaa ccacgttcac cggattggtg gagccatagc acttggccag gacgttctgc acaccagcca cttccagaac ggcacgcatg gcgccgccgg cgatgatgcc ggtaccttcc gaagccggct gcatgtacac cttggaggcg ccatgggcgg acttggtcgg atactgcagg gtggtgccgt tcagatcgac ttggatcatg ttgcggcgag cggcttccat tgctttctgg atggcggccg gcacttcacg cgccttgcca cgaccgaaac caacgcgacc cttgccatca cccaccacgg tcagtgcggt gaaagcgaag atacggccac cttttacggt tttggcgacg cggttaacct gaaccagctt ctcgatgtag ccttcgtcgc gcttttgctc gttgtttgcc atatggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA NNEQKRDEGY IEKLVQVNRV AKTVKGGRIF AFTALTVVGD GKGRVGFGRG KAREVPAAIQ KAMEAARRNM IQVDLNGTTL QYPTKSAHGA SKVYMQPASE GTGIIAGGAM RAVLEVAGVQ NVLAKCYGST NPVNVVYATF KGLKNMQAPE AVAAKRGKSV EEIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa acaacgagca aaagcgcgac gaaggctaca tcgagaagct ggttcaggtt aaccgcgtcg ccaaaaccgt aaaaggtggc cgtatcttcg ctttcaccgc actgaccgtg gtgggtgatg gcaagggtcg cgttggtttc ggtcgtggca aggcgcgtga agtgccggcc gccatccaga aagcaatgga agccgctcgc cgcaacatga tccaagtcga tctgaacggc accaccctgc agtatccgac caagtccgcc catggcgcct ccaaggtgta catgcagccg gcttcggaag gtaccggcat catcgccggc ggcgccatgc gtgccgttct ggaagtggct ggtgtgcaga acgtcctggc caagtgctat ggctccacca atccggtgaa cgtggtttac gccaccttca agggcctgaa gaacatgcag gctccggaag cagtagcggc caagcgtggc aagagcgtcg aggagattct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat