SSGCID: Target Page

PsaeA.17974.a

Protein translocase subunit SecA

CENTER ID:	PsaeA.17974.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17974.a.B1.GE38504	full length		1	916
PsaeA.17974.a.B3.GE38505	SecA wing/scaffold		617	840

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17974.a.B3.PS02306	SecA wing/scaffold		617	840

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.4611
PGAT:	pseudomonas:PA4403
RefSeq:	NP_253093.1
UniProt:	Q9LCT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFAPLLKKLF GSKNERDVKR MAKAVQAINA LEPQMVALSD EQLKAKTAEF QQRYAKGETL DQLLPEAFAV VREAGKRVMG MRHFDVQLIG GMTLHDGKIA EMRTGEGKTL VGTLPVYLNA LSGKGVHVVT VNDYLARRDA NWMRPLYEFL GLSVGVVTPF QPPEDKRAAY AADITYGTNN EFGFDYLRDN MAFSLDDKFQ RELNFAVVDE VDSILIDEAR TPLIISGQAE DSSELYIKIN KLIPRLKRQV EEVEGKPTEE GHYSIDEKTR QVELNEQGHQ FIEDLLSQNG LLGEGESLYS AHNLSLLTHV YAALRAHTLF HRNVEYIVQG DQILLIDEHT GRTMPGRRLS EGLHQAIEAK EGLPIQAESQ TLASTTFQNY FRLYNKLAGM TGTADTEAFE FRQIYGLDVV VIPTHRPIAR KDFNDLVYLT QEEKYAAIIT DIKQCQALGR PILVGTASIE SSEYVSKLLQ EAGIEHKVLN AKYHEKEAEI IAQAGAPGSV TIATNMAGRG TDILLGGNWE VEVAALENPT EEQIAQIKAE WQKRHQQVIE AGGLHVIASE RHESRRIDNQ LRGRAGRQGD PGSSRFYLSL EDNLMRIFAS DRVKNFMKAL GMQSGEAIEH RMVTNAIEKA QRKVEGRNFD IRKQLLEFDD VANEQRKVIY HMRNTLLSAE DVGETIKEFR EETLSATINQ HIPPQSLPEQ WDIEGLEAAL YSDFAVRLPI QQWLDEDDKL YEETLRSKIL EQIVAAYYEK EELAGAEALR AFEKQMLLRV LDDLWKDHLS TMDHLRHGIH LRGYAQKNPK QEYKRESFTL FQELLDSIKR DTIRVLSHVQ VRREDPAEEE ARLRREAEEL AKRMQFQHAE APSMEQAVAG EEEELPEGPA PVVPLEPVRN EQKIGRNEPC PCGSGKKYKH CHGQLD

NT Sequence

atgtttgcgc ctttgttgaa gaaactcttt ggaagcaaga acgagcgtga tgtaaagcgc atggccaagg ctgtacaggc catcaatgcg ctcgagcccc agatggtggc gctctccgac gagcagttga aggcgaaaac ggctgaattc cagcagcgct acgccaaggg cgaaacgctc gaccaattgc tgcccgaagc cttcgccgtg gttcgcgagg ctggcaagcg ggtgatgggc atgcgccact tcgatgtcca gctgatcggt ggcatgaccc tgcacgacgg caagatcgcc gaaatgcgta ccggcgaggg caagaccctg gtgggcaccc tgccggtcta cctcaacgcg ctgtccggca agggcgtgca cgtggtcacg gtgaacgact acctggcgcg ccgcgacgcc aactggatgc gcccgctgta cgagttcctc ggcctcagcg tcggcgtggt cactcccttc cagccgccgg aagacaagcg cgccgcctac gccgccgaca tcacctacgg caccaacaac gaattcggct tcgattacct gcgcgacaac atggccttca gcctggacga caagttccag cgcgagctga acttcgccgt ggtcgacgaa gtggactcga tcctcatcga cgaggcgcgt accccgctga tcatctccgg ccaggccgaa gacagctccg agctgtacat caagatcaac aagctgatcc cgcgtctcaa gcgccaggtt gaagaggtcg agggcaagcc gaccgaggaa ggccactaca gcatcgacga gaagacccgc caggtcgaac tcaacgagca gggccaccag ttcatcgagg acctgctgag ccagaacggc ctgctcggcg aaggcgagag cctctactcg gcgcacaacc tgagcctgct gacccacgtc tacgcagcgc tgcgcgcgca caccctgttc caccgcaacg tcgagtacat cgtccagggc gaccagatcc tcctgatcga cgagcacacc ggccgcacca tgcctggccg acgcctgtcc gagggcctgc accaggccat cgaggcgaag gaaggcttgc cgatccaggc cgagagccag accctggcct ccaccacctt ccagaactac ttccgcctgt acaacaagct ggccggcatg accggcaccg ccgataccga ggccttcgag ttccgccaga tctacggtct cgacgtggtg gtgatcccga cccaccggcc gatcgcgcgc aaggacttca acgacctggt ctacctgacc caggaagaga agtacgcggc gatcatcacc gacatcaagc agtgccaggc cctcggccgg ccgatcctgg tcggcaccgc gtcgatcgag agttccgagt acgtttccaa gctgttgcaa gaggcaggca tcgagcacaa ggtgctcaac gccaagtacc acgagaagga agccgagatc atcgcccagg ctggcgcgcc cggttcggtg accatcgcca ccaacatggc cggccgcggt accgacatcc tcctcggcgg caactgggaa gtcgaagtcg cggccctgga gaaccccacc gaggagcaga tcgcccagat caaggccgaa tggcagaagc gccaccagca ggtgatcgag gcgggcggcc tgcacgtgat cgcctccgag cgccacgaat cccgccggat cgacaaccag ttgcgcggcc gtgccggtcg tcagggcgac ccgggttcca gccgcttcta cctgtcgctg gaagacaacc tgatgcggat cttcgcctcc gaccgggtga agaacttcat gaaggcgctg ggcatgcagt ccggcgaggc gatcgagcac cgcatggtga ccaacgccat cgagaaggcc cagcgcaagg tcgaagggcg caacttcgac atccgcaagc aactgctgga attcgatgac gtagccaacg agcagcgcaa ggtgatctac cacatgcgca ataccctgct gtcggcagag gacgtgggcg agaccatcaa ggaattccgc gaggaaaccc tcagcgccac catcaaccag cacattccgc cgcagtcgct gcccgagcag tgggacatcg aaggcctgga ggctgcgctg tacagcgact tcgccgtgcg cctgccgatc cagcagtggc tggacgaaga cgacaagctg tacgaggaaa ccctgcgtag caagatcctc gagcagatcg tcgccgccta ctacgagaag gaagagctgg ccggcgccga agccctgcgc gccttcgaga agcagatgct gctgcgggta ctggacgacc tgtggaagga ccacctgtcg accatggacc acctgcggca cggcatccac ctgcgtggct acgcgcagaa gaatccgaag caggaataca agcgcgagtc cttcaccctg ttccaggagc tgctggactc gatcaagcgc gacaccatcc gcgtgctgtc ccacgtccag gtccgtcgcg aagacccggc cgaagaggag gcccgcctgc gtcgcgaggc ggaggaactg gccaagcgca tgcagttcca gcacgccgag gctccctcga tggagcaggc cgtggccggc gaggaagaag agcttcccga gggcccggct ccggtcgtgc cgctcgagcc tgtgcgcaac gagcagaaga tcggccgcaa cgagccttgc ccgtgcggtt ccggcaagaa atacaagcac tgccacgggc agctggacta a

Details for PsaeA.17974.a.B1.GE38504

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	105kDa
OBSERVED MW:	105kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	91
PERCENT COVERAGE:	30

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgttt gcgcctttgt tgaagaaact ctttggaagc aagaacgagc gtgatgtaaa gcgcatggcc aaggctgtac aggccatcaa tgcgctcgag ccccagatgg tggcgctctc cgacgagcag ttgaaggcga aaacggctga attccagcag cgctacgcca agggcgaaac gctcgaccaa ttgctgcccg aagccttcgc cgtggttcgc gaggctggca agcgggtgat gggcatgcgc cacttcgatg tccagctgat cggtggcatg accctgcacg acggcaagat cgccgaaatg cgtaccggcg agggcaagac cctggtgggc accctgccgg tctacctcaa cgcgctgtcc ggcaagggcg tgcacgtggt cacggtgaac gactacctgg cgcgccgcga cgccaactgg atgcgcccgc tgtacgagtt cctcggcctc agcgtcggcg tggtcactcc cttccagccg ccggaagaca agcgcgccgc ctacgccgcc gacatcacct acggcaccaa caacgaattc ggcttcgatt acctgcgcga caacatggcc ttcagcctgg acgacaagtt ccagcgcgag ctgaacttcg ccgtggtcga cgaagtggac tcgatcctca tcgacgaggc gcgtaccccg ctgatcatct ccggccaggc cgaagacagc tccgagctgt acatcaagnt naanaagctg atcccgcgtc tcaagcgcca ggttnnnnnn ntnnnnngcn nnnnnnnnna nnannnnnnn nnnnnnnnnn nnnnnnnnnn nncnnnnngg

Expected Protein Sequence

MAHHHHHHMF APLLKKLFGS KNERDVKRMA KAVQAINALE PQMVALSDEQ LKAKTAEFQQ RYAKGETLDQ LLPEAFAVVR EAGKRVMGMR HFDVQLIGGM TLHDGKIAEM RTGEGKTLVG TLPVYLNALS GKGVHVVTVN DYLARRDANW MRPLYEFLGL SVGVVTPFQP PEDKRAAYAA DITYGTNNEF GFDYLRDNMA FSLDDKFQRE LNFAVVDEVD SILIDEARTP LIISGQAEDS SELYIKINKL IPRLKRQVEE VEGKPTEEGH YSIDEKTRQV ELNEQGHQFI EDLLSQNGLL GEGESLYSAH NLSLLTHVYA ALRAHTLFHR NVEYIVQGDQ ILLIDEHTGR TMPGRRLSEG LHQAIEAKEG LPIQAESQTL ASTTFQNYFR LYNKLAGMTG TADTEAFEFR QIYGLDVVVI PTHRPIARKD FNDLVYLTQE EKYAAIITDI KQCQALGRPI LVGTASIESS EYVSKLLQEA GIEHKVLNAK YHEKEAEIIA QAGAPGSVTI ATNMAGRGTD ILLGGNWEVE VAALENPTEE QIAQIKAEWQ KRHQQVIEAG GLHVIASERH ESRRIDNQLR GRAGRQGDPG SSRFYLSLED NLMRIFASDR VKNFMKALGM QSGEAIEHRM VTNAIEKAQR KVEGRNFDIR KQLLEFDDVA NEQRKVIYHM RNTLLSAEDV GETIKEFREE TLSATINQHI PPQSLPEQWD IEGLEAALYS DFAVRLPIQQ WLDEDDKLYE ETLRSKILEQ IVAAYYEKEE LAGAEALRAF EKQMLLRVLD DLWKDHLSTM DHLRHGIHLR GYAQKNPKQE YKRESFTLFQ ELLDSIKRDT IRVLSHVQVR REDPAEEEAR LRREAEELAK RMQFQHAEAP SMEQAVAGEE EELPEGPAPV VPLEPVRNEQ KIGRNEPCPC GSGKKYKHCH GQLD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg cgcctttgtt gaagaaactc tttggaagca agaacgagcg tgatgtaaag cgcatggcca aggctgtaca ggccatcaat gcgctcgagc cccagatggt ggcgctctcc gacgagcagt tgaaggcgaa aacggctgaa ttccagcagc gctacgccaa gggcgaaacg ctcgaccaat tgctgcccga agccttcgcc gtggttcgcg aggctggcaa gcgggtgatg ggcatgcgcc acttcgatgt ccagctgatc ggtggcatga ccctgcacga cggcaagatc gccgaaatgc gtaccggcga gggcaagacc ctggtgggca ccctgccggt ctacctcaac gcgctgtccg gcaagggcgt gcacgtggtc acggtgaacg actacctggc gcgccgcgac gccaactgga tgcgcccgct gtacgagttc ctcggcctca gcgtcggcgt ggtcactccc ttccagccgc cggaagacaa gcgcgccgcc tacgccgccg acatcaccta cggcaccaac aacgaattcg gcttcgatta cctgcgcgac aacatggcct tcagcctgga cgacaagttc cagcgcgagc tgaacttcgc cgtggtcgac gaagtggact cgatcctcat cgacgaggcg cgtaccccgc tgatcatctc cggccaggcc gaagacagct ccgagctgta catcaagatc aacaagctga tcccgcgtct caagcgccag gttgaagagg tcgagggcaa gccgaccgag gaaggccact acagcatcga cgagaagacc cgccaggtcg aactcaacga gcagggccac cagttcatcg aggacctgct gagccagaac ggcctgctcg gcgaaggcga gagcctctac tcggcgcaca acctgagcct gctgacccac gtctacgcag cgctgcgcgc gcacaccctg ttccaccgca acgtcgagta catcgtccag ggcgaccaga tcctcctgat cgacgagcac accggccgca ccatgcctgg ccgacgcctg tccgagggcc tgcaccaggc catcgaggcg aaggaaggct tgccgatcca ggccgagagc cagaccctgg cctccaccac cttccagaac tacttccgcc tgtacaacaa gctggccggc atgaccggca ccgccgatac cgaggccttc gagttccgcc agatctacgg tctcgacgtg gtggtgatcc cgacccaccg gccgatcgcg cgcaaggact tcaacgacct ggtctacctg acccaggaag agaagtacgc ggcgatcatc accgacatca agcagtgcca ggccctcggc cggccgatcc tggtcggcac cgcgtcgatc gagagttccg agtacgtttc caagctgttg caagaggcag gcatcgagca caaggtgctc aacgccaagt accacgagaa ggaagccgag atcatcgccc aggctggcgc gcccggttcg gtgaccatcg ccaccaacat ggccggccgc ggtaccgaca tcctcctcgg cggcaactgg gaagtcgaag tcgcggccct ggagaacccc accgaggagc agatcgccca gatcaaggcc gaatggcaga agcgccacca gcaggtgatc gaggcgggcg gcctgcacgt gatcgcctcc gagcgccacg aatcccgccg gatcgacaac cagttgcgcg gccgtgccgg tcgtcagggc gacccgggtt ccagccgctt ctacctgtcg ctggaagaca acctgatgcg gatcttcgcc tccgaccggg tgaagaactt catgaaggcg ctgggcatgc agtccggcga ggcgatcgag caccgcatgg tgaccaacgc catcgagaag gcccagcgca aggtcgaagg gcgcaacttc gacatccgca agcaactgct ggaattcgat gacgtagcca acgagcagcg caaggtgatc taccacatgc gcaataccct gctgtcggca gaggacgtgg gcgagaccat caaggaattc cgcgaggaaa ccctcagcgc caccatcaac cagcacattc cgccgcagtc gctgcccgag cagtgggaca tcgaaggcct ggaggctgcg ctgtacagcg acttcgccgt gcgcctgccg atccagcagt ggctggacga agacgacaag ctgtacgagg aaaccctgcg tagcaagatc ctcgagcaga tcgtcgccgc ctactacgag aaggaagagc tggccggcgc cgaagccctg cgcgccttcg agaagcagat gctgctgcgg gtactggacg acctgtggaa ggaccacctg tcgaccatgg accacctgcg gcacggcatc cacctgcgtg gctacgcgca gaagaatccg aagcaggaat acaagcgcga gtccttcacc ctgttccagg agctgctgga ctcgatcaag cgcgacacca tccgcgtgct gtcccacgtc caggtccgtc gcgaagaccc ggccgaagag gaggcccgcc tgcgtcgcga ggcggaggaa ctggccaagc gcatgcagtt ccagcacgcc gaggctccct cgatggagca ggccgtggcc ggcgaggaag aagagcttcc cgagggcccg gctccggtcg tgccgctcga gcctgtgcgc aacgagcaga agatcggccg caacgagcct tgcccgtgcg gttccggcaa gaaatacaag cactgccacg ggcagctgga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for PsaeA.17974.a.B3.GE38505

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHHM AIEHRMVTNA IEKAQRKVEG RNFDIRKQLL EFDDVANEQR KVIYHMRNTL LSAEDVGETI KEFREETLSA TINQHIPPQS LPEQWDIEGL EAALYSDFAV RLPIQQWLDE DDKLYEETLR SKILEQIVAA YYEKEELAGA EALRAFEKQM LLRVLDDLWK DHLSTMDHLR HGIHLRGYAQ KNPKQEYKRE SFTLFQELLD SIKRDTIRVL SHVQVRREDP AEEE

Validated NT Sequence

atggctcacc accaccacca ccaccatatg gcgatcgagc accgcatggt gaccaacgcc atcgagaagg cccagcgcaa ggtcgaaggg cgcaacttcg acatccgcaa gcaactgctg gaattcgatg acgtagccaa cgagcagcgc aaggtgatct accacatgcg caataccctg ctgtcggcag aggacgtggg cgagaccatc aaggaattcc gcgaggaaac cctcagcgcc accatcaacc agcacattcc gccgcagtcg ctgcccgagc agtgggacat cgaaggcctg gaggctgcgc tgtacagcga cttcgccgtg cgcctgccga tccagcagtg gctggacgaa gacgacaagc tgtacgagga aaccctgcgt agcaagatcc tcgagcagat cgtcgccgcc tactacgaga aggaagagct ggccggcgcc gaagccctgc gcgccttcga gaagcagatg ctgctgcggg tactggacga cctgtggaag gaccacctgt cgaccatgga ccacctgcgg cacggcatcc acctgcgtgg ctacgcgcag aagaatccga agcaggaata caagcgcgag tccttcaccc tgttccagga gctgctggac tcgatcaagc gcgacaccat ccgcgtgctg tcccacgtcc aggtccgtcg cgaagacccg gccgaagagg agtaagtgag taagat

Expected Protein Sequence

MAHHHHHHAI EHRMVTNAIE KAQRKVEGRN FDIRKQLLEF DDVANEQRKV IYHMRNTLLS AEDVGETIKE FREETLSATI NQHIPPQSLP EQWDIEGLEA ALYSDFAVRL PIQQWLDEDD KLYEETLRSK ILEQIVAAYY EKEELAGAEA LRAFEKQMLL RVLDDLWKDH LSTMDHLRHG IHLRGYAQKN PKQEYKRESF TLFQELLDSI KRDTIRVLSH VQVRREDPAE EE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgatcg agcaccgcat ggtgaccaac gccatcgaga aggcccagcg caaggtcgaa gggcgcaact tcgacatccg caagcaactg ctggaattcg atgacgtagc caacgagcag cgcaaggtga tctaccacat gcgcaatacc ctgctgtcgg cagaggacgt gggcgagacc atcaaggaat tccgcgagga aaccctcagc gccaccatca accagcacat tccgccgcag tcgctgcccg agcagtggga catcgaaggc ctggaggctg cgctgtacag cgacttcgcc gtgcgcctgc cgatccagca gtggctggac gaagacgaca agctgtacga ggaaaccctg cgtagcaaga tcctcgagca gatcgtcgcc gcctactacg agaaggaaga gctggccggc gccgaagccc tgcgcgcctt cgagaagcag atgctgctgc gggtactgga cgacctgtgg aaggaccacc tgtcgaccat ggaccacctg cggcacggca tccacctgcg tggctacgcg cagaagaatc cgaagcagga atacaagcgc gagtccttca ccctgttcca ggagctgctg gactcgatca agcgcgacac catccgcgtg ctgtcccacg tccaggtccg tcgcgaagac ccggccgaag aggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for PsaeA.17974.a.B3.PS02306

PURIFICATION DATe:	1/20/2015
CONCENTRATION:	29.6mg/ml
OBSERVED MW:	28kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGATCG AGCACCGCAT GGTGACCAAC GCCATCGAGA AGGCCCAGCG CAAGGTCGAA GGGCGCAACT TCGACATCCG CAAGCAACTG CTGGAATTCG ATGACGTAGC CAACGAGCAG CGCAAGGTGA TCTACCACAT GCGCAATACC CTGCTGTCGG CAGAGGACGT GGGCGAGACC ATCAAGGAAT TCCGCGAGGA AACCCTCAGC GCCACCATCA ACCAGCACAT TCCGCCGCAG TCGCTGCCCG AGCAGTGGGA CATCGAAGGC CTGGAGGCTG CGCTGTACAG CGACTTCGCC GTGCGCCTGC CGATCCAGCA GTGGCTGGAC GAAGACGACA AGCTGTACGA GGAAACCCTG CGTAGCAAGA TCCTCGAGCA GATCGTCGCC GCCTACTACG AGAAGGAAGA GCTGGCCGGC GCCGAAGCCC TGCGCGCCTT CGAGAAGCAG ATGCTGCTGC GGGTACTGGA CGACCTGTGG AAGGACCACC TGTCGACCAT GGACCACCTG CGGCACGGCA TCCACCTGCG TGGCTACGCG CAGAAGAATC CGAAGCAGGA ATACAAGCGC GAGTCCTTCA CCCTGTTCCA GGAGCTGCTG GACTCGATCA AGCGCGACAC CATCCGCGTG CTGTCCCACG TCCAGGTCCG TCGCGAAGAC CCGGCCGAAG AGGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT