SSGCID: Target Page

PsaeA.17966.a

Succinate dehydrogenase (B subunit)

CENTER ID:	PsaeA.17966.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17966.a.B1.GE38500	full length		1	235

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.1643
PGAT:	pseudomonas:PA1584
RefSeq:	NP_250275.1
UniProt:	Q9I3D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQVSVYRYN PDKDAAPYMQ DFQVDTNGKD VMVLDVLALI KEQDEGFSYR RSCREGVCGS DGMNINGKNG LACITPLSAA GLKGGKLVIR PLPGLPVIRD LVVDMSIFYK QYEKVKPFLQ NDTPAPAIER LQTPEEREKL DGLYECILCA CCSTSCPSFW WNPDKFLGPA ALLQAYRFLA DSRDTKTEER LASLDDPFSV FRCRGIMNCV NVCPKGLNPT KAIGHVRNML LQSGT

NT Sequence

atgttgcaag tgagtgttta tcgttacaac cccgacaaag atgctgcgcc gtacatgcag gatttccagg tcgacaccaa cggcaaggac gtgatggtcc tcgacgtgct ggcgctgatc aaggaacagg acgagggctt ctcctaccgt cgttcctgcc gtgaaggcgt ctgcggctcc gatggcatga acatcaacgg caagaacggc ctggcctgca tcaccccgct gtcggccgcc ggcctgaagg gcggcaagct ggtgatccgt ccgctgccgg gcctgccggt cattcgtgac ctggtcgtgg acatgagcat cttctacaag caatacgaga aggtgaagcc gttcctgcag aacgatacgc cggccccggc catcgagcgt ctgcagaccc cggaagagcg cgagaagctg gacggtctgt acgagtgcat cctgtgcgcc tgctgctcga cctcctgccc gtccttctgg tggaaccccg acaagttcct cggtccggcc gcactgctgc aagcctatcg tttcctggcc gacagccgtg acaccaagac cgaagagcgc ctggcttcgc tggacgaccc gttcagtgtg ttccgctgcc gcggaatcat gaactgcgtg aacgtttgcc cgaagggtct gaacccgacc aaggcgatcg gccacgtgcg taatatgctg ctgcagagcg gaacctga

Details for PsaeA.17966.a.B1.GE38500

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML QVSVYRYNPD KDAAPYMQDF QVDTNGKDVM VLDVLALIKE QDEGFSYRRS CREGVCGSDG MNINGKNGLA CITPLSAAGL KGGKLVIRPL PGLPVIRDLV VDMSIFYKQY EKVKPFLQND TPAPAIERLQ TPEEREKLDG LYECILCACC STSCPSFWWN PDKFLGPAAL LQAYRFLADS RDTKTEERLA SLDDPFSVFR CRGIMNCVNV CPKGLNPTKA IGHVRNMLLQ SGT

Validated NT Sequence

atcttactca cttaggttcc gctctgcagc agcatattac gcacgtggcc gatcgccttg gtcgggttca gacccttcgg gcaaacgttc acgcagttca tgattccgcg gcagcggaac acactgaacg ggtcgtccag cgaagccagg cgctcttcgg tcttggtgtc acggctgtcg gccaggaaac gataggcttg cagcagtgcg gccggaccga ggaacttgtc ggggttccac cagaaggacg ggcaggaggt cgagcagcag gcgcacagga tgcactcgta cagaccgtcc agcttctcgc gctcttccgg ggtctgcaga cgctcgatgg ccggggccgg cgtatcgttc tgcaggaacg gcttcacctt ctcgtattgc ttgtagaaga tgctcatgtc cacgaccagg tcacgaatga ccggcaggcc cggcagcgga cggatcacca gcttgccgcc cttcaggccg gcggccgaca gcggggtgat gcaggccagg ccgttcttgc cgttgatgtt catgccatcg gagccgcaga cgccttcacg gcaggaacga cggtaggaga agccctcgtc ctgttccttg atcagcgcca gcacgtcgag gaccatcacg tccttgccgt tggtgtcgac ctggaaatcc tgcatgtacg gcgcagcatc tttgtcgggg ttgtaacgat aaacactcac ttgcaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc aagtgagtgt ttatcgttac aaccccgaca aagatgctgc gccgtacatg caggatttcc aggtcgacac caacggcaag gacgtgatgg tcctcgacgt gctggcgctg atcaaggaac aggacgaggg cttctcctac cgtcgttcct gccgtgaagg cgtctgcggc tccgatggca tgaacatcaa cggcaagaac ggcctggcct gcatcacccc gctgtcggcc gccggcctga agggcggcaa gctggtgatc cgtccgctgc cgggcctgcc ggtcattcgt gacctggtcg tggacatgag catcttctac aagcaatacg agaaggtgaa gccgttcctg cagaacgata cgccggcccc ggccatcgag cgtctgcaga ccccggaaga gcgcgagaag ctggacggtc tgtacgagtg catcctgtgc gcctgctgct cgacctcctg cccgtccttc tggtggaacc ccgacaagtt cctcggtccg gccgcactgc tgcaagccta tcgtttcctg gccgacagcc gtgacaccaa gaccgaagag cgcctggctt cgctggacga cccgttcagt gtgttccgct gccgcggaat catgaactgc gtgaacgttt gcccgaaggg tctgaacccg accaaggcga tcggccacgt gcgtaatatg ctgctgcaga gcggaacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat