SSGCID: Target Page

PsaeA.17954.a

Lipid-A-disaccharide synthase (EC 2.4.1.182)

CENTER ID:	PsaeA.17954.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17954.a.B1.GE38496	full length		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17954.a.B1.PW37611	full length		1	378

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.3812
PGAT:	pseudomonas:PA3643
RefSeq:	NP_252333.1
UniProt:	Q9HXY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADGLRVALV AGEASGDILG SGLMQALRAR HPDIEFIGVG GPRMEAEGLS SYFPMERLSV MGLVEVLGRL PELLRRRKRL IRTLIEARPD VMIGIDAPDF TLGVEHKLRQ AGLRTVHYVS PSVWAWRQKR VLKIREACDL MLALFPFEAR FYEEHGVPVR FVGHPLANTI PLQADRAAAR ARLGLPADGQ VLALMPGSRG GEVGKLGALF LDTAQRLLVE RPGLRFVLPC ASAARREQIE QMLQGREPLP LTLLDGASHE ALAACDAVLI ASGTATLEAL LYKRPMVVAY RVAGLTYRIL KRLVKSPYIS LPNLLAGRLL VPELIQDAAT PQALAATLSP LLDDGSQQVE FFDAIHRALR QDASAQAAEA VLQLVERR

NT Sequence

atggctgacg gattacgcgt agccctggtc gccggcgagg cctccggcga catcctcggg tccggactga tgcaggcgct gcgggcgcgg cacccggata tcgagttcat cggcgtcggt gggccgcgca tggaggccga agggctgtcc agctatttcc ccatggagcg cctgtcggtg atgggcctgg tagaagtgct gggcaggctg ccggagttgt tgcgacggcg caagcgcctg atccgcacgc tcatcgaggc ccggccggat gtgatgatcg gcatcgacgc tcccgacttc accctcggcg tcgaacacaa gctgcgccag gctggcctgc gcaccgtgca ttacgtcagc ccttcggtgt gggcctggcg gcagaagcgg gtgctgaaga ttcgcgaagc ctgcgacctg atgctggcat tgttcccctt cgaagcacgt ttctacgaag agcatggggt gcccgtgcgt ttcgtcggcc accccctggc caacaccatt cccctgcagg cggatcgcgc tgcggcccgc gcgcggctcg gattgccggc ggacggacag gtgctggcgc tgatgccggg cagtcgcggc ggcgaggtcg gcaagctcgg cgccttgttc ctcgacaccg cccagcgcct gctcgtcgaa cggccgggcc tgcgtttcgt gctgccctgc gccagcgctg cacggcgcga acagatcgag cagatgctcc agggccgcga gcctctgccg ctgacgctgc tcgacggcgc ttcccatgaa gcgcttgccg cctgcgatgc ggtgctgatc gcctccggta ccgcgaccct cgaggcgctg ctctacaagc ggcccatggt ggtggcttat cgggtcgccg ggctgacgta ccggattctc aagcgcctgg tgaaaagccc ttacatctcc ctgccgaacc tgttggccgg acgcctgttg gtgccggaac tgatccagga cgccgccacc ccgcaagcgc tagccgcaac cctctcgccg ctgctggacg acggctcgca gcaggtggaa ttcttcgatg ccatccatcg ggccttgcgc caggatgctt cggcacaggc ggcggaagcc gtcctgcaac tggtggagcg ccgctga

Details for PsaeA.17954.a.B1.GE38496

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA DGLRVALVAG EASGDILGSG LMQALRARHP DIEFIGVGGP RMEAEGLSSY FPMERLSVMG LVEVLGRLPE LLRRRKRLIR TLIEARPDVM IGIDAPDFTL GVEHKLRQAG LRTVHYVSPS VWAWRQKRVL KIREACDLML ALFPFEARFY EEHGVPVRFV GHPLANTIPL QADRAAARAR LGLPADGQVL ALMPGSRGGE VGKLGALFLD TAQRLLVERP GLRFVLPCAS AARREQIEQM LQGREPLPLT LLDGASHEAL AACDAVLIAS GTATLEALLY KRPMVVAYRV AGLTYRILKR LVKSPYISLP NLLAGRLLVP ELIQDAATPQ ALAATLSPLL DDGSQQVEFF DAIHRXXRQD ASAQAA

Validated NT Sequence

ttccgccgcc tgtgccgaag catcctggcg cnnnnnccga tggatggcat cgaagaattc cacctgctgc gagccgtcgt ccagcagcgg cgagagggtt gcggctagcg cttgcggggt ggcggcgtcc tggatcagtt ccggcaccaa caggcgtccg gccaacaggt tcggcaggga gatgtaaggg cttttcacca ggcgcttgag aatccggtac gtcagcccgg cgacccgata agccaccacc atgggccgct tgtagagcag cgcctcgagg gtcgcggtac cggaggcgat cagcaccgca tcgcaggcgg caagcgcttc atgggaagcg ccgtcgagca gcgtcagcgg cagaggctcg cggccctgga gcatctgctc gatctgttcg cgccgtgcag cgctggcgca gggcagcacg aaacgcaggc ccggccgttc gacgagcagg cgctgggcgg tgtcgaggaa caaggcgccg agcttgccga cctcgccgcc gcgactgccc ggcatcagcg ccagcacctg tccgtccgcc ggcaatccga gccgcgcgcg ggccgcagcg cgatccgcct gcaggggaat ggtgttggcc agggggtggc cgacgaaacg cacgggcacc ccatgctctt cgtagaaacg tgcttcgaag gggaacaatg ccagcatcag gtcgcaggct tcgcgaatct tcagcacccg cttctgccgc caggcccaca ccgaagggct gacgtaatgc acggtgcgca ggccagcctg gcgcagcttg tgttcgacgc cgagggtgaa gtcgggagcg tcgatgccga tcatcacatc cggccgggcc tcgatgagcg tgcggatcag gcgcttgcgc cgtcgcaaca actccggcag cctgcccagc acttctacca ggcccatcac cgacaggcgc tccatgggga aatagctgga cagcccttcg gcctccatgc gcggcccacc gacgccgatg aactcgatat ccgggtgccg cgcccgcagc gcctgcatca gtccggaccc gaggatgtcg ccggaggcct cgccggcgac cagggctacg cgtaatccgt cagccatatg gtggtggtgg tggtgagcca tggtatnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg acggattacg cgtagccctg gtcgccggcg aggcctccgg cgacatcctc gggtccggac tgatgcaggc gctgcgggcg cggcacccgg atatcgagtt catcggcgtc ggtgggccgc gcatggaggc cgaagggctg tccagctatt tccccatgga gcgcctgtcg gtgatgggcc tggtagaagt gctgggcagg ctgccggagt tgttgcgacg gcgcaagcgc ctgatccgca cgctcatcga ggcccggccg gatgtgatga tcggcatcga cgctcccgac ttcaccctcg gcgtcgaaca caagctgcgc caggctggcc tgcgcaccgt gcattacgtc agcccttcgg tgtgggcctg gcggcagaag cgggtgctga agattcgcga agcctgcgac ctgatgctgg cattgttccc cttcgaagca cgtttctacg aagagcatgg ggtgcccgtg cgtttcgtcg gccaccccct ggccaacacc attcccctgc aggcggatcg cgctgcggcc cgcgcgcggc tcggattgcc ggcggacgga caggtgctgg cgctgatgcc gggcagtcgc ggcggcgagg tcggcaagct cggcgccttg ttcctcgaca ccgcccagcg cctgctcgtc gaacggccgg gcctgcgttt cgtgctgccc tgcgccagcg ctgcacggcg cgaacagatc gagcagatgc tccagggccg cgagcctctg ccgctgacgc tgctcgacgg cgcttcccat gaagcgcttg ccgcctgcga tgcggtgctg atcgcctccg gtaccgcgac cctcgaggcg ctgctctaca agcggcccat ggtggtggct tatcgggtcg ccgggctgac gtaccggatt ctcaagcgcc tggtgaaaag cccttacatc tccctgccga acctgttggc cggacgcctg ttggtgccgg aactgatcca ggacgccgcc accccgcaag cgctagccgc aaccctctcg ccgctgctgg acgacggctc gcagcaggtg gaattcttcg atgccatcca tcgggccttg cgccaggatg cttcggcaca ggcggcggaa gccgtcctgc aactggtgga gcgccgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for PsaeA.17954.a.B1.PW37611

PURIFICATION DATe:	12/2/2014
CONCENTRATION:	6mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG ACGGATTACG CGTAGCCCTG GTCGCCGGCG AGGCCTCCGG CGACATCCTC GGGTCCGGAC TGATGCAGGC GCTGCGGGCG CGGCACCCGG ATATCGAGTT CATCGGCGTC GGTGGGCCGC GCATGGAGGC CGAAGGGCTG TCCAGCTATT TCCCCATGGA GCGCCTGTCG GTGATGGGCC TGGTAGAAGT GCTGGGCAGG CTGCCGGAGT TGTTGCGACG GCGCAAGCGC CTGATCCGCA CGCTCATCGA GGCCCGGCCG GATGTGATGA TCGGCATCGA CGCTCCCGAC TTCACCCTCG GCGTCGAACA CAAGCTGCGC CAGGCTGGCC TGCGCACCGT GCATTACGTC AGCCCTTCGG TGTGGGCCTG GCGGCAGAAG CGGGTGCTGA AGATTCGCGA AGCCTGCGAC CTGATGCTGG CATTGTTCCC CTTCGAAGCA CGTTTCTACG AAGAGCATGG GGTGCCCGTG CGTTTCGTCG GCCACCCCCT GGCCAACACC ATTCCCCTGC AGGCGGATCG CGCTGCGGCC CGCGCGCGGC TCGGATTGCC GGCGGACGGA CAGGTGCTGG CGCTGATGCC GGGCAGTCGC GGCGGCGAGG TCGGCAAGCT CGGCGCCTTG TTCCTCGACA CCGCCCAGCG CCTGCTCGTC GAACGGCCGG GCCTGCGTTT CGTGCTGCCC TGCGCCAGCG CTGCACGGCG CGAACAGATC GAGCAGATGC TCCAGGGCCG CGAGCCTCTG CCGCTGACGC TGCTCGACGG CGCTTCCCAT GAAGCGCTTG CCGCCTGCGA TGCGGTGCTG ATCGCCTCCG GTACCGCGAC CCTCGAGGCG CTGCTCTACA AGCGGCCCAT GGTGGTGGCT TATCGGGTCG CCGGGCTGAC GTACCGGATT CTCAAGCGCC TGGTGAAAAG CCCTTACATC TCCCTGCCGA ACCTGTTGGC CGGACGCCTG TTGGTGCCGG AACTGATCCA GGACGCCGCC ACCCCGCAAG CGCTAGCCGC AACCCTCTCG CCGCTGCTGG ACGACGGCTC GCAGCAGGTG GAATTCTTCG ATGCCATCCA TCGGGCCTTG CGCCAGGATG CTTCGGCACA GGCGGCGGAA GCCGTCCTGC AACTGGTGGA GCGCCGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT