SSGCID: Target Page

PsaeA.17951.a

Aspartokinase (EC 2.7.2.4) (Aspartate kinase)

CENTER ID:	PsaeA.17951.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17951.a.B1.GE38494	full length		1	412

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.939
PGAT:	pseudomonas:PA0904
RefSeq:	NP_249595.1
UniProt:	O69077

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALIVQKFGG TSVGTVERIE QVAEKVKKFR EAGDDVVVVV SAMSGETNRL IGLANQIMEQ PVPRELDVMV STGEQVTIAL LSMALIKRGV PAVSYTGNQV RILTDSAHTK ARILHIDDTH IRADLKAGRV VVVAGFQGVD GNGNITTLGR GGSDTTGVAL AAALKADECQ IYTDVDGVYT TDPRVVPQAR RLDKITFEEM LEMASLGSKV LQIRAVEFAG KYNVPLRVLH SFQEGPGTLI TIDDEEESME QPIISGIAFN RDEAKLTIRG VPDTPGVAFK ILGPISAANV EVDMIVQNVA HDNTTDFTFT VHRNDYLNAL EILKQTAANI GAREAIGDTN IAKVSIVGVG MRSHAGVASR MFEALAKESI NIQMISTSEI KVSVVIEEKY LELAVRALHT AFELDAPARQ GE

NT Sequence

ATGGCTTTGA TCGTACAGAA GTTTGGAGGG ACCTCCGTCG GCACCGTCGA GCGTATCGAG CAGGTGGCCG AGAAGGTGAA GAAATTCCGC GAAGCGGGCG ATGACGTCGT CGTGGTGGTA TCCGCCATGA GCGGGGAGAC CAACCGTCTG ATCGGCCTGG CCAACCAGAT CATGGAGCAG CCGGTTCCGC GCGAACTGGA CGTCATGGTC TCGACCGGAG AGCAGGTGAC CATCGCCCTG CTCAGCATGG CCCTGATCAA GCGCGGCGTG CCGGCGGTCT CCTATACCGG CAACCAGGTG CGTATCCTCA CCGACAGCGC GCACACCAAG GCGCGCATCC TGCACATCGA CGACACCCAC ATCCGCGCCG ACCTCAAGGC CGGCAGGGTA GTGGTGGTCG CCGGTTTCCA GGGCGTCGAC GGCAACGGCA ACATCACCAC GCTGGGCCGC GGCGGTTCCG ATACCACCGG GGTGGCCCTG GCGGCGGCGT TGAAGGCCGA CGAATGCCAG ATCTACACCG ACGTCGACGG CGTCTACACC ACCGATCCGC GCGTAGTGCC GCAGGCCCGT CGGCTGGACA AGATCACCTT CGAGGAAATG CTGGAAATGG CCAGCCTCGG TTCCAAGGTG CTGCAGATCC GGGCGGTCGA GTTCGCCGGC AAGTACAACG TTCCGCTGCG CGTGCTGCAC AGCTTCCAGG AGGGTCCGGG CACCCTCATT ACTATCGATG ATGAAGAGGA ATCCATGGAA CAGCCGATCA TCTCCGGCAT CGCCTTCAAC CGCGACGAAG CCAAGCTGAC CATCCGTGGC GTACCCGATA CCCCCGGCGT GGCCTTCAAG ATCCTCGGGC CGATCAGTGC CGCCAATGTG GAAGTCGACA TGATCGTGCA GAACGTGGCG CACGATAACA CCACCGACTT CACCTTCACG GTGCATCGCA ACGATTACCT GAACGCCCTG GAAATCCTCA AGCAGACCGC CGCCAACATC GGCGCGCGCG AAGCCATCGG CGACACCAAC ATCGCCAAGG TTTCCATCGT CGGCGTCGGC ATGCGTTCGC ACGCCGGCGT GGCCAGCCGC ATGTTCGAAG CGCTGGCCAA GGAAAGCATC AACATCCAGA TGATCTCCAC CTCGGAAATC AAGGTTTCCG TGGTGATCGA AGAGAAGTAC CTGGAACTGG CGGTACGTGC TCTGCACACG GCGTTCGAGC TCGACGCGCC GGCGCGACAG GGTGAGTGA

Details for PsaeA.17951.a.B1.GE38494

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA LIVQKFGGTS VGTVERIEQV AEKVKKFREA GDDVVVVVSA MSGETNRLIG LANQIMEQPV PRELDVMVST GEQVTIALLS MALIKRGVPA VSYTGNQVRI LTDSAHTKAR ILHIDDTHIR ADLKAGRVVV VAGFQGVDGN GNITTLGRGG SDTTGVALAA ALKADECQIY TDVDGVYTTD PRVVPQARRL DKITFEEMLE MASLGSKVLQ IRAVEFAGKY NVPLRVLHSF QEGPGTLITI DDEEESMEQP IISGIAFNRD EAKLTIRGVP DTPGVAFKIL GPISAANVEV DMIVQNVAHD NTTDFTFTVH RNDYLNALEI LKQTAANIGA REAIGDTNIA KVSIVGVGMR SHAGVASRMF EALAKESINI QMISTSEIKV SVVIEEKYLE LAVRALHTAF EL

Validated NT Sequence

cgagctcgaa cgccgtgtgc agagcacgta ccgccagttc caggtacttc tcttcgatca ccacggaaac cttgatttcc gaggtggaga tcatctggat gttgatgctt tccttggcca gcgcttcgaa catgcggctg gccacgccgg cgtgcgaacg catgccgacg ccgacgatgg aaaccttggc gatgttggtg tcgccgatgg cttcgcgcgc gccgatgttg gcggcggtct gcttgaggat ttccagggcg ttcaggtaat cgttgcgatg caccgtgaag gtgaagtcgg tggtgttatc gtgcgccacg ttctgcacga tcatgtcgac ttccacattg gcggcactga tcggcccgag gatcttgaag gccacgccgg gggtatcggg tacgccacgg atggtcagct tggcttcgtc gcggttgaag gcgatgccgg agatgatcgg ctgttccatg gattcctctt catcatcgat agtaatgagg gtgcccggac cctcctggaa gctgtgcagc acgcgcagcg gaacgttgta cttgccggcg aactcgaccg cccggatctg cagcaccttg gaaccgaggc tggccatttc cagcatttcc tcgaaggtga tcttgtccag ccgacgggcc tgcggcacta cgcgcggatc ggtggtgtag acgccgtcga cgtcggtgta gatctggcat tcgtcggcct tcaacgccgc cgccagggcc accccggtgg tatcggaacc gccgcggccc agcgtggtga tgttgccgtt gccgtcgacg ccctggaaac cggcgaccac cactaccctg ccggccttga ggtcggcgcg gatgtgggtg tcgtcgatgt gcaggatgcg cgccttggtg tgcgcgctgt cggtgaggat acgcacctgg ttgccggtat aggagaccgc cggcacgccg cgcttgatca gggccatgct gagcagggcg atggtcacct gctctccggt cgagaccatg acgtccagtt cgcgcggaac cggctgctcc atgatctggt tggccaggcc gatcagacgg ttggtctccc cgctcatggc ggataccacc acgacgacgt catcgcccgc ttcgcggaat ttcttcacct tctcggccac ctgctcgata cgctcgacgg tgccgacgga ggtccctcca aacttctgta cgatcaaagc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt tgatcgtaca gaagtttgga gggacctccg tcggcaccgt cgagcgtatc gagcaggtgg ccgagaaggt gaagaaattc cgcgaagcgg gcgatgacgt cgtcgtggtg gtatccgcca tgagcgggga gaccaaccgt ctgatcggcc tggccaacca gatcatggag cagccggttc cgcgcgaact ggacgtcatg gtctcgaccg gagagcaggt gaccatcgcc ctgctcagca tggccctgat caagcgcggc gtgccggcgg tctcctatac cggcaaccag gtgcgtatcc tcaccgacag cgcgcacacc aaggcgcgca tcctgcacat cgacgacacc cacatccgcg ccgacctcaa ggccggcagg gtagtggtgg tcgccggttt ccagggcgtc gacggcaacg gcaacatcac cacgctgggc cgcggcggtt ccgataccac cggggtggcc ctggcggcgg cgttgaaggc cgacgaatgc cagatctaca ccgacgtcga cggcgtctac accaccgatc cgcgcgtagt gccgcaggcc cgtcggctgg acaagatcac cttcgaggaa atgctggaaa tggccagcct cggttccaag gtgctgcaga tccgggcggt cgagttcgcc ggcaagtaca acgttccgct gcgcgtgctg cacagcttcc aggagggtcc gggcaccctc attactatcg atgatgaaga ggaatccatg gaacagccga tcatctccgg catcgccttc aaccgcgacg aagccaagct gaccatccgt ggcgtacccg atacccccgg cgtggccttc aagatcctcg ggccgatcag tgccgccaat gtggaagtcg acatgatcgt gcagaacgtg gcgcacgata acaccaccga cttcaccttc acggtgcatc gcaacgatta cctgaacgcc ctggaaatcc tcaagcagac cgccgccaac atcggcgcgc gcgaagccat cggcgacacc aacatcgcca aggtttccat cgtcggcgtc ggcatgcgtt cgcacgccgg cgtggccagc cgcatgttcg aagcgctggc caaggaaagc atcaacatcc agatgatctc cacctcggaa atcaaggttt ccgtggtgat cgaagagaag tacctggaac tggcggtacg tgctctgcac acggcgttcg agctcgacgc gccggcgcga cagggtgagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat