PsaeA.17945.a

30S ribosomal protein S3

CENTER ID: PsaeA.17945.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17945.a.B1.GE38493 full length 1 228
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4458
PGAT: pseudomonas:PA4257
RefSeq: NP_252947.1
UniProt: Q9HWE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGQKVHPNGI RLGIVKEHTS VWYADRKNYA DYLFADLKVR EYLQDKLKSA SVSRIDIHRP AQTARITIHT ARPGIVIGKK GEDVEKLRQD LTKQMGVPVH INIEEIRKPE LDAMLVAQSV AQQLERRVMF RRAMKRAVQN AMRIGAKGIK IQVSGRLGGA EIARTEWYRE GRVPLHTLRA DIDYATYEAH TTYGVIGVKV WIFKGEVIGG RQEELKPVAP APRKKAAR
NT Sequence
atgggtcaga aagtacatcc caatggcatt cgcctgggta tcgtcaagga gcacacctcc gtttggtacg cagaccgcaa gaactatgcg gattacctgt ttgccgacct gaaggtgcgt gagtacctcc aagacaaact aaaaagcgcg tccgtaagcc gtatcgatat tcatcgtccg gctcaaaccg cacgcatcac catccacacc gctcgtcccg gcatcgtgat cggcaagaaa ggtgaagatg ttgagaaact gcgccaggac ctgaccaagc aaatgggtgt gcccgtgcac atcaacatcg aggaaatccg caagccggaa ctcgatgcca tgctggttgc gcagagcgta gcccagcagc tggagcgtcg cgtaatgttc cgtcgcgcca tgaagcgcgc cgtacagaac gccatgcgca ttggtgccaa gggcatcaag attcaggtca gcggtcgtct gggtggggct gaaatcgccc gtaccgaatg gtatcgcgaa ggtcgtgtgc ccctgcacac cctgcgcgcc gatatcgact atgccaccta cgaagcgcac accacttacg gtgtgatcgg tgtcaaggtt tggatcttca agggcgaggt catcggtggc cgccaggaag agctgaagcc ggtagcaccc gctcctcgta aaaaagctgc taggtaa
Details for PsaeA.17945.a.B1.GE38493
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG QKVHPNGIRL GIVKEHTSVW YADRKNYADY LFADLKVREY LQDKLKSASV SRIDIHRPAQ TARITIHTAR PGIVIGKKGE DVEKLRQDLT KQMGVPVHIN IEEIRKPELD AMLVAQSVAQ QLERRVMFRR AMKRAVQNAM RIGAKGIKIQ VSGRLGGAEI ARTEWYREGR VPLHTLRADI DYATYEAHTT YGVIGVKVWI FKGEVIGGRQ EELKPVAPAP XKKAAR
Validated NT Sequence
atcttactca cttacctagc agctttttta nnaggagcgg gtgctaccgg cttcagctct tcctggcggc caccgatgac ctcgcccttg aagatccaaa ccttgacacc gatcacaccg taagtggtgt gcgcttcgta ggtggcatag tcgatatcgg cgcgcagggt gtgcaggggc acacgacctt cgcgatacca ttcggtacgg gcgatttcag ccccacccag acgaccgctg acctgaatct tgatgccctt ggcaccaatg cgcatggcgt tctgtacggc gcgcttcatg gcgcgacgga acattacgcg acgctccagc tgctgggcta cgctctgcgc aaccagcatg gcatcgagtt ccggcttgcg gatttcctcg atgttgatgt gcacgggcac acccatttgc ttggtcaggt cctggcgcag tttctcaaca tcttcacctt tcttgccgat cacgatgccg ggacgagcgg tgtggatggt gatgcgtgcg gtttgagccg gacgatgaat atcgatacgg cttacggacg cgctttttag tttgtcttgg aggtactcac gcaccttcag gtcggcaaac aggtaatccg catagttctt gcggtctgcg taccaaacgg aggtgtgctc cttgacgata cccaggcgaa tgccattggg atgtactttc tgacccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMG QKVHPNGIRL GIVKEHTSVW YADRKNYADY LFADLKVREY LQDKLKSASV SRIDIHRPAQ TARITIHTAR PGIVIGKKGE DVEKLRQDLT KQMGVPVHIN IEEIRKPELD AMLVAQSVAQ QLERRVMFRR AMKRAVQNAM RIGAKGIKIQ VSGRLGGAEI ARTEWYREGR VPLHTLRADI DYATYEAHTT YGVIGVKVWI FKGEVIGGRQ EELKPVAPAP RKKAAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc agaaagtaca tcccaatggc attcgcctgg gtatcgtcaa ggagcacacc tccgtttggt acgcagaccg caagaactat gcggattacc tgtttgccga cctgaaggtg cgtgagtacc tccaagacaa actaaaaagc gcgtccgtaa gccgtatcga tattcatcgt ccggctcaaa ccgcacgcat caccatccac accgctcgtc ccggcatcgt gatcggcaag aaaggtgaag atgttgagaa actgcgccag gacctgacca agcaaatggg tgtgcccgtg cacatcaaca tcgaggaaat ccgcaagccg gaactcgatg ccatgctggt tgcgcagagc gtagcccagc agctggagcg tcgcgtaatg ttccgtcgcg ccatgaagcg cgccgtacag aacgccatgc gcattggtgc caagggcatc aagattcagg tcagcggtcg tctgggtggg gctgaaatcg cccgtaccga atggtatcgc gaaggtcgtg tgcccctgca caccctgcgc gccgatatcg actatgccac ctacgaagcg cacaccactt acggtgtgat cggtgtcaag gtttggatct tcaagggcga ggtcatcggt ggccgccagg aagagctgaa gccggtagca cccgctcctc gtaaaaaagc tgctaggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at