PsaeA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID: PsaeA.17943.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17943.a.B1.GE38492 full length 1 873
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17943.a.B1.PW37600 full length 1 873

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4180
PGAT: pseudomonas:PA3987
RefSeq: NP_252676.1
UniProt: Q9HX33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHEQYQPLEI ETQAQNYWKE HQSFLVRELP DKEKFYCLSM FPYPSGKLHM GHVRNYTIGD VISRYHRMQG RNVLQPMGWD AFGMPAENAA MKNNVAPAAW TYDNIAYMKS QLDSLGLAID WSREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAE ELLESLDNLP GWPEQVKTMQ RNWIGKSRGM EIGFPYDQAS IGHAGQLKVF TTRPDTLMGA TYVAVAAEHP LATQAAQNDP QLQAFIDECK RGGVAEADIA TQEKKGMATS LFVEHPLTGD KLPVWVANYV LMNYGEGAVM AVPGHDERDF EFANKYGLPI RQVIAKVEGD NDFESSVWKE WYGAKDESVL TVNSGKYDNL GYQAAFDAIG ADLEAKGLGQ ARTQFRLRDW GISRQRYWGC PIPIIHCEAC GDVPVPADQL PVVLPEDVVP DGSGSPLAKM PEFYECNCPK CGQPAKRETD TMDTFVESSW YFARYACPQF EGGMLDRKAA DYWLPVDQYI GGIEHAILHL LYARFFHKLM RDEGLVGSDE PFKNLLTQGM VVADTYYRTT ANGGKDWFNP ADVEVERDAK AKVVGARLKS DGQPVEIGGT EKMSKSKNNG VDPQSMIDQY GADTCRLFMM FASPPDMSLE WSDAGVEGAN RFLRRVWRLA HAHVSAGLPG ALDVAALSDA QKQVRRAIHL AIRQASQDVG QHHKFNTAIA AVMTLMNVLE KAPNQDAQDR ALIQEGLETV VLLLAPITPH ICHVLWGQLG HAEAVIDARW PSVDESALVQ DTLQLVVQVN GKLRGHIDVA ASASREDVEA AARANENVLR FTEGLSIRKV IVVPGKLVNI VAN
NT Sequence
atgcacgagc aatatcagcc actcgagatc gaaacccagg cccagaacta ctggaaagaa caccagtcct tcctcgtcag ggagctaccc gacaaggaga agttctactg cctgtcgatg ttcccgtatc cgagcggcaa gctgcacatg ggccacgtgc gcaactacac catcggcgac gtgatcagcc gctaccaccg catgcagggc cgcaacgtgc tgcagccgat gggctgggac gcgttcggca tgccggcgga aaacgccgcg atgaagaaca acgtcgcccc ggcggcctgg acctacgaca acatcgccta catgaagtcg cagctggaca gcctcggcct ggcgatcgac tggagccgcg aggtcaccac ctgcaagccg gactactacc gctgggagca gtggctgttc acccgcctgt tcgaaaaagg ggtgatctac cgcaagaacg gcacggtcaa ttgggatccg gtcgaccaga ccgtactcgc caacgagcag gtgatcgacg ggcgcggctg gcgctccggc gcgctgatcg agaagcgcga gatcccgatg tactacttca agatcaccgc ctacgccgag gaactgctgg agagcctcga caacctgccc ggctggccgg agcaggtgaa gaccatgcag cgcaactgga tcggcaagtc gcgcgggatg gagatcggct tcccctacga ccaggcctcc atcggccacg ccggccaact gaaggtgttc accacccgtc cggacaccct gatgggcgcc acctacgtcg ccgtcgccgc cgaacacccg ctggccaccc aggcagcgca gaacgacccg cagttgcagg cgttcatcga cgaatgcaag cgcggcggcg tcgccgaggc cgacatcgcc acccaggaga agaaaggcat ggccacttcc ctgttcgtcg aacacccgct gaccggcgac aagctgccgg tatgggtcgc caactacgtg ctgatgaact acggcgaagg cgcggtgatg gcggtgcccg gccatgacga gcgcgacttc gagttcgcca acaagtacgg cctgccgatc cgccaggtga tcgccaaggt cgagggcgac aacgacttcg agtccagcgt ctggaaggag tggtacggcg ccaaggacga gtccgtgctc accgtcaact ccggcaagta cgacaacctc ggctaccagg ccgccttcga cgccatcggc gccgacctcg aggccaaggg cctgggccag gcgcgcaccc agttccgcct gcgcgactgg ggcatcagcc gccagcgcta ctggggctgc ccgatcccga tcatccactg cgaggcttgc ggcgacgtcc cggtgccggc cgaccagttg ccggtggtgc tgccggaaga cgtggtaccg gacggctccg gttcgccgct ggcgaagatg ccggaattct acgaatgcaa ctgcccgaaa tgcggccagc cggccaagcg cgaaaccgac accatggaca ccttcgtcga gtcgtcctgg tatttcgccc gctacgcctg cccgcagttc gaaggcggca tgctggacag gaaggcggcc gactactggc tgccggtcga ccagtacatc ggcggcatcg agcacgccat cctgcacctg ctctatgcgc gcttcttcca caagctgatg cgcgacgaag gcctggtcgg cagcgacgaa ccgttcaaga acctgctgac ccagggcatg gtggtcgccg atacctacta ccggaccacc gccaacggcg gcaaggactg gttcaacccg gccgacgtcg aggtcgagcg tgacgccaag gccaaggtcg tcggcgcgcg cctgaagagc gacggccagc cggtcgagat cggcggcacc gagaagatgt cgaagtcgaa gaacaacggt gtcgacccgc aatcgatgat cgaccagtac ggtgccgata cctgccgcct gttcatgatg ttcgcctcgc cgccggacat gagcctggag tggtcggacg ccggcgtcga gggcgccaac cgcttcctcc gccgcgtctg gcgcctggct cacgcccacg tcagcgccgg cctccccggc gcgctggacg tcgccgccct gagcgacgcg cagaagcagg tccgccgcgc catccacctg gcgatcaggc aggccagcca ggatgtcggc cagcaccaca agttcaacac cgccatcgcc gcggtgatga ccctgatgaa cgtgctggag aaggcgccga accaggacgc ccaggaccgc gccctgatcc aggaaggctt ggaaaccgtg gtcctgctgc tggcgcccat cactccgcac atctgccacg tgctctgggg ccagctcggc catgccgaag cggtgatcga tgcgcgctgg ccgagcgtcg acgagagcgc actggtgcag gacaccctgc aactggtggt gcaggtcaac ggcaagctgc gcggacacat cgacgtggcc gccagcgcca gccgcgagga tgtcgaggcc gccgcgcgcg ccaacgagaa cgtgctgcgt ttcaccgaag gcctgagcat ccgcaaggtc atcgtggtac cgggcaaact ggtcaacatc gtcgccaact ga
Details for PsaeA.17943.a.B1.GE38492
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 99kDa
OBSERVED MW: 99kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMH EQYQPLEIET QAQNYWKEHQ SFLVRELPDK EKFYCLSMFP YPSGKLHMGH VRNYTIGDVI SRYHRMQGRN VLQPMGWDAF GMPAENAAMK NNVAPAAWTY DNIAYMKSQL DSLGLAIDWS REVTTCKPDY YRWEQWLFTR LFEKGVIYRK NGTVNWDPVD QTVLANEQVI DGRGWRSGAL IEKREIPMYY FKITAYAEEL LESLDNLPGW PEQVKTMQRN WIGKSRGMEI GFPYDQASIG HAGQLKVFTT RPDTLMGATY VAVAAEHPLA TQAAQNDPQL QAFIDECKRG GVAEADIATQ EKKGMATSLF VEHPLTGDKL PVWVANYVLM NYGEGAVMAV PGHDERDFEF ANKYGLPIRQ VIAKVEGDND FESSVWKEWY GAKDESVLTV NSGKYDNLGY QAAFDAIGAD LEAKGLGQAR TQFRLRDWGI SRQRYWGCPI PIIHCEACGD VPVPADQLPV VLPEDVVPDG SGSPLAKMPE FYECNCPKCG QPAKRETDTM DTFVESSWYF ARYACPQFEG GMLDRKAADY WLPVDQYIGG IEHAILHLLY ARFFHKLMRD EGLVGSDEPF KNLLTQGMVV ADTYYRTTAN GGKDWFNPAD VEVERDAKAK VVGARLKSDG QPVEIGGTEK MSKSKNNGVD PQSMIDQYGA DTCRLFMMFA SPPDMSLEWS DAGVEGANRF LRRVWRLAHA HVSAGLPGAL DVAALSDAQK QVRRAIHLAI RQASQDVGQH HKFNTAIAAV MTLMNVLEKA PNQDAQDRAL IQEGLETVVL LLAPITPHIC HVLWGQLGHA EAVIDARWPS VDESALVQDT LQLVVQVNGK LRGHIDVAAS ASREDVEAAA RANENVLRFT EGLSIRKVIV VPGKLVNIVA N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacg agcaatatca gccactcgag atcgaaaccc aggcccagaa ctactggaaa gaacaccagt ccttcctcgt cagggagcta cccgacaagg agaagttcta ctgcctgtcg atgttcccgt atccgagcgg caagctgcac atgggccacg tgcgcaacta caccatcggc gacgtgatca gccgctacca ccgcatgcag ggccgcaacg tgctgcagcc gatgggctgg gacgcgttcg gcatgccggc ggaaaacgcc gcgatgaaga acaacgtcgc cccggcggcc tggacctacg acaacatcgc ctacatgaag tcgcagctgg acagcctcgg cctggcgatc gactggagcc gcgaggtcac cacctgcaag ccggactact accgctggga gcagtggctg ttcacccgcc tgttcgaaaa aggggtgatc taccgcaaga acggcacggt caattgggat ccggtcgacc agaccgtact cgccaacgag caggtgatcg acgggcgcgg ctggcgctcc ggcgcgctga tcgagaagcg cgagatcccg atgtactact tcaagatcac cgcctacgcc gaggaactgc tggagagcct cgacaacctg cccggctggc cggagcaggt gaagaccatg cagcgcaact ggatcggcaa gtcgcgcggg atggagatcg gcttccccta cgaccaggcc tccatcggcc acgccggcca actgaaggtg ttcaccaccc gtccggacac cctgatgggc gccacctacg tcgccgtcgc cgccgaacac ccgctggcca cccaggcagc gcagaacgac ccgcagttgc aggcgttcat cgacgaatgc aagcgcggcg gcgtcgccga ggccgacatc gccacccagg agaagaaagg catggccact tccctgttcg tcgaacaccc gctgaccggc gacaagctgc cggtatgggt cgccaactac gtgctgatga actacggcga aggcgcggtg atggcggtgc ccggccatga cgagcgcgac ttcgagttcg ccaacaagta cggcctgccg atccgccagg tgatcgccaa ggtcgagggc gacaacgact tcgagtccag cgtctggaag gagtggtacg gcgccaagga cgagtccgtg ctcaccgtca actccggcaa gtacgacaac ctcggctacc aggccgcctt cgacgccatc ggcgccgacc tcgaggccaa gggcctgggc caggcgcgca cccagttccg cctgcgcgac tggggcatca gccgccagcg ctactggggc tgcccgatcc cgatcatcca ctgcgaggct tgcggcgacg tcccggtgcc ggccgaccag ttgccggtgg tgctgccgga agacgtggta ccggacggct ccggttcgcc gctggcgaag atgccggaat tctacgaatg caactgcccg aaatgcggcc agccggccaa gcgcgaaacc gacaccatgg acaccttcgt cgagtcgtcc tggtatttcg cccgctacgc ctgcccgcag ttcgaaggcg gcatgctgga caggaaggcg gccgactact ggctgccggt cgaccagtac atcggcggca tcgagcacgc catcctgcac ctgctctatg cgcgcttctt ccacaagctg atgcgcgacg aaggcctggt cggcagcgac gaaccgttca agaacctgct gacccagggc atggtggtcg ccgataccta ctaccggacc accgccaacg gcggcaagga ctggttcaac ccggccgacg tcgaggtcga gcgtgacgcc aaggccaagg tcgtcggcgc gcgcctgaag agcgacggcc agccggtcga gatcggcggc accgagaaga tgtcgaagtc gaagaacaac ggtgtcgacc cgcaatcgat gatcgaccag tacggtgccg atacctgccg cctgttcatg atgttcgcct cgccgccgga catgagcctg gagtggtcgg acgccggcgt cgagggcgcc aaccgcttcc tccgccgcgt ctggcgcctg gctcacgccc acgtcagcgc cggcctcccc ggcgcgctgg acgtcgccgc cctgagcgac gcgcagaagc aggtccgccg cgccatccac ctggcgatca ggcaggccag ccaggatgtc ggccagcacc acaagttcaa caccgccatc gccgcggtga tgaccctgat gaacgtgctg gagaaggcgc cgaaccagga cgcccaggac cgcgccctga tccaggaagg cttggaaacc gtggtcctgc tgctggcgcc catcactccg cacatctgcc acgtgctctg gggccagctc ggccatgccg aagcggtgat cgatgcgcgc tggccgagcg tcgacgagag cgcactggtg caggacaccc tgcaactggt ggtgcaggtc aacggcaagc tgcgcggaca catcgacgtg gccgccagcg ccagccgcga ggatgtcgag gccgccgcgc gcgccaacga gaacgtgctg cgtttcaccg aaggcctgag catccgcaag gtcatcgtgg taccgggcaa actggtcaac atcgtcgcca actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for PsaeA.17943.a.B1.PW37600
PURIFICATION DATe: 11/18/2014
CONCENTRATION: 25mg/ml
OBSERVED MW: 97kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMH EQYQPLEIET QAQNYWKEHQ SFLVRELPDK EKFYCLSMFP YPSGKLHMGH VRNYTIGDVI SRYHRMQGRN VLQPMGWDAF GMPAENAAMK NNVAPAAWTY DNIAYMKSQL DSLGLAIDWS REVTTCKPDY YRWEQWLFTR LFEKGVIYRK NGTVNWDPVD QTVLANEQVI DGRGWRSGAL IEKREIPMYY FKITAYAEEL LESLDNLPGW PEQVKTMQRN WIGKSRGMEI GFPYDQASIG HAGQLKVFTT RPDTLMGATY VAVAAEHPLA TQAAQNDPQL QAFIDECKRG GVAEADIATQ EKKGMATSLF VEHPLTGDKL PVWVANYVLM NYGEGAVMAV PGHDERDFEF ANKYGLPIRQ VIAKVEGDND FESSVWKEWY GAKDESVLTV NSGKYDNLGY QAAFDAIGAD LEAKGLGQAR TQFRLRDWGI SRQRYWGCPI PIIHCEACGD VPVPADQLPV VLPEDVVPDG SGSPLAKMPE FYECNCPKCG QPAKRETDTM DTFVESSWYF ARYACPQFEG GMLDRKAADY WLPVDQYIGG IEHAILHLLY ARFFHKLMRD EGLVGSDEPF KNLLTQGMVV ADTYYRTTAN GGKDWFNPAD VEVERDAKAK VVGARLKSDG QPVEIGGTEK MSKSKNNGVD PQSMIDQYGA DTCRLFMMFA SPPDMSLEWS DAGVEGANRF LRRVWRLAHA HVSAGLPGAL DVAALSDAQK QVRRAIHLAI RQASQDVGQH HKFNTAIAAV MTLMNVLEKA PNQDAQDRAL IQEGLETVVL LLAPITPHIC HVLWGQLGHA EAVIDARWPS VDESALVQDT LQLVVQVNGK LRGHIDVAAS ASREDVEAAA RANENVLRFT EGLSIRKVIV VPGKLVNIVA N
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACG AGCAATATCA GCCACTCGAG ATCGAAACCC AGGCCCAGAA CTACTGGAAA GAACACCAGT CCTTCCTCGT CAGGGAGCTA CCCGACAAGG AGAAGTTCTA CTGCCTGTCG ATGTTCCCGT ATCCGAGCGG CAAGCTGCAC ATGGGCCACG TGCGCAACTA CACCATCGGC GACGTGATCA GCCGCTACCA CCGCATGCAG GGCCGCAACG TGCTGCAGCC GATGGGCTGG GACGCGTTCG GCATGCCGGC GGAAAACGCC GCGATGAAGA ACAACGTCGC CCCGGCGGCC TGGACCTACG ACAACATCGC CTACATGAAG TCGCAGCTGG ACAGCCTCGG CCTGGCGATC GACTGGAGCC GCGAGGTCAC CACCTGCAAG CCGGACTACT ACCGCTGGGA GCAGTGGCTG TTCACCCGCC TGTTCGAAAA AGGGGTGATC TACCGCAAGA ACGGCACGGT CAATTGGGAT CCGGTCGACC AGACCGTACT CGCCAACGAG CAGGTGATCG ACGGGCGCGG CTGGCGCTCC GGCGCGCTGA TCGAGAAGCG CGAGATCCCG ATGTACTACT TCAAGATCAC CGCCTACGCC GAGGAACTGC TGGAGAGCCT CGACAACCTG CCCGGCTGGC CGGAGCAGGT GAAGACCATG CAGCGCAACT GGATCGGCAA GTCGCGCGGG ATGGAGATCG GCTTCCCCTA CGACCAGGCC TCCATCGGCC ACGCCGGCCA ACTGAAGGTG TTCACCACCC GTCCGGACAC CCTGATGGGC GCCACCTACG TCGCCGTCGC CGCCGAACAC CCGCTGGCCA CCCAGGCAGC GCAGAACGAC CCGCAGTTGC AGGCGTTCAT CGACGAATGC AAGCGCGGCG GCGTCGCCGA GGCCGACATC GCCACCCAGG AGAAGAAAGG CATGGCCACT TCCCTGTTCG TCGAACACCC GCTGACCGGC GACAAGCTGC CGGTATGGGT CGCCAACTAC GTGCTGATGA ACTACGGCGA AGGCGCGGTG ATGGCGGTGC CCGGCCATGA CGAGCGCGAC TTCGAGTTCG CCAACAAGTA CGGCCTGCCG ATCCGCCAGG TGATCGCCAA GGTCGAGGGC GACAACGACT TCGAGTCCAG CGTCTGGAAG GAGTGGTACG GCGCCAAGGA CGAGTCCGTG CTCACCGTCA ACTCCGGCAA GTACGACAAC CTCGGCTACC AGGCCGCCTT CGACGCCATC GGCGCCGACC TCGAGGCCAA GGGCCTGGGC CAGGCGCGCA CCCAGTTCCG CCTGCGCGAC TGGGGCATCA GCCGCCAGCG CTACTGGGGC TGCCCGATCC CGATCATCCA CTGCGAGGCT TGCGGCGACG TCCCGGTGCC GGCCGACCAG TTGCCGGTGG TGCTGCCGGA AGACGTGGTA CCGGACGGCT CCGGTTCGCC GCTGGCGAAG ATGCCGGAAT TCTACGAATG CAACTGCCCG AAATGCGGCC AGCCGGCCAA GCGCGAAACC GACACCATGG ACACCTTCGT CGAGTCGTCC TGGTATTTCG CCCGCTACGC CTGCCCGCAG TTCGAAGGCG GCATGCTGGA CAGGAAGGCG GCCGACTACT GGCTGCCGGT CGACCAGTAC ATCGGCGGCA TCGAGCACGC CATCCTGCAC CTGCTCTATG CGCGCTTCTT CCACAAGCTG ATGCGCGACG AAGGCCTGGT CGGCAGCGAC GAACCGTTCA AGAACCTGCT GACCCAGGGC ATGGTGGTCG CCGATACCTA CTACCGGACC ACCGCCAACG GCGGCAAGGA CTGGTTCAAC CCGGCCGACG TCGAGGTCGA GCGTGACGCC AAGGCCAAGG TCGTCGGCGC GCGCCTGAAG AGCGACGGCC AGCCGGTCGA GATCGGCGGC ACCGAGAAGA TGTCGAAGTC GAAGAACAAC GGTGTCGACC CGCAATCGAT GATCGACCAG TACGGTGCCG ATACCTGCCG CCTGTTCATG ATGTTCGCCT CGCCGCCGGA CATGAGCCTG GAGTGGTCGG ACGCCGGCGT CGAGGGCGCC AACCGCTTCC TCCGCCGCGT CTGGCGCCTG GCTCACGCCC ACGTCAGCGC CGGCCTCCCC GGCGCGCTGG ACGTCGCCGC CCTGAGCGAC GCGCAGAAGC AGGTCCGCCG CGCCATCCAC CTGGCGATCA GGCAGGCCAG CCAGGATGTC GGCCAGCACC ACAAGTTCAA CACCGCCATC GCCGCGGTGA TGACCCTGAT GAACGTGCTG GAGAAGGCGC CGAACCAGGA CGCCCAGGAC CGCGCCCTGA TCCAGGAAGG CTTGGAAACC GTGGTCCTGC TGCTGGCGCC CATCACTCCG CACATCTGCC ACGTGCTCTG GGGCCAGCTC GGCCATGCCG AAGCGGTGAT CGATGCGCGC TGGCCGAGCG TCGACGAGAG CGCACTGGTG CAGGACACCC TGCAACTGGT GGTGCAGGTC AACGGCAAGC TGCGCGGACA CATCGACGTG GCCGCCAGCG CCAGCCGCGA GGATGTCGAG GCCGCCGCGC GCGCCAACGA GAACGTGCTG CGTTTCACCG AAGGCCTGAG CATCCGCAAG GTCATCGTGG TACCGGGCAA ACTGGTCAAC ATCGTCGCCA ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT