SSGCID: Target Page

PsaeA.17941.a

tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.-)

CENTER ID:	PsaeA.17941.a
ORGANISM:	Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE:	Pseudomonas infection
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17941.a.B1.GE38490	full length		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
PsaeA.17941.a.B1.PW37608	full length		1	375

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|208964.12.peg.2748
PGAT:	pseudomonas:PA2626
RefSeq:	NP_251316.1
UniProt:	Q9I0L2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSESAPTPRR ERVIVGMSGG VDSSVSALLL LQQGYQVEGL FMKNWDEDDG TEYCTAREDL ADAQAVCDRI GIKLHTANFA AEYWDNVFEH FLAEYKAGRT PNPDILCNRE IKFKAFLDYA LMLGADLIAT GHYVRRRDRD GRTELLKGLD PNKDQSYFLH AVGGEQIARS LFPVGELEKP EVRAIAEKHG LATAKKKDST GICFIGERRF TDFLKQYLPA QPGDIETTEG KVIGRHSGLM YHTIGQRQGL GIGGLKEAGD DPWYVLGKDL QRNVLLVGQG NDHPLLFSRA LLASRIYWVN PVELERPRRL RAKVRYRQSD QDCVLEKTAE GYRAVFDEPQ RAVTPGQSVV FYDGDVCLGG GVIETAEAWD FGGRP

NT Sequence

atgtctgaat ccgcccccac cccacgccgc gagcgcgtga tcgtcggcat gtccggcggc gtcgactcgt ccgtctccgc cctgctcctg ttgcagcagg gctatcaggt cgaaggcctg ttcatgaaga actgggacga ggacgacggc accgaatact gtaccgcccg cgaggacctg gccgacgccc aggccgtctg cgaccggatc ggcatcaagc tgcacacggc gaacttcgcc gcggaatact gggataacgt gttcgagcac ttccttgccg agtacaaggc cggccgtacg ccgaacccgg acatcctctg caaccgcgaa atcaagttca aggcgttcct cgactacgcg ctgatgctcg gcgcggacct gatcgccacc ggccactatg tgcggcgccg cgaccgcgac gggcgcaccg agctgctcaa gggcctggac ccgaacaagg accagagcta cttcctgcac gcggtgggcg gcgagcagat cgcccgcagc ctgttccccg tcggcgaact ggagaagccg gaagtccgcg ccatcgccga gaagcatggc ctcgccacgg cgaagaagaa ggactccacg ggcatctgct tcattggcga gcgacgcttc accgatttcc tcaagcagta cctgccggcc cagccgggcg acatcgagac caccgaaggc aaggtcatcg gtcgccacag cggcctgatg taccacacca tcggccagcg ccagggcctc ggcatcggcg gtctcaagga agccggcgac gatccctggt acgtgctcgg caaggacctg caacgcaacg tgctgctggt cggccagggc aacgaccacc cgctgctgtt ctcccgcgcc ctgctggcct cgcgaatcta ctgggtcaac ccggtcgaac tggagcggcc gcgccgcctg cgggccaagg tgcgctaccg gcagagcgac caggactgcg tcctggagaa aaccgccgaa ggctaccgcg cggtgttcga cgaaccgcag cgcgcagtga ccccggggca atcggtggtg ttctacgacg gcgacgtctg cctcggcggc ggcgtgatcg aaacggcgga agcctgggat ttcggaggac gcccgtga

Details for PsaeA.17941.a.B1.GE38490

HARVESTED ON:	10/9/2014
SEQUENCED ON:	11/24/2014
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS ESAPTPRRER VIVGMSGGVD SSVSALLLLQ QGYQVEGLFM KNWDEDDGTE YCTAREDLAD AQAVCDRIGI KLHTANFAAE YWDNVFEHFL AEYKAGRTPN PDILCNREIK FKAFLDYALM LGADLIATGH YVRRRDRDGR TELLKGLDPN KDQSYFLHAV GGEQIARSLF PVGELEKPEV RAIAEKHGLA TAKKKDSTGI CFIGERRFTD FLKQYLPAQP GDIETTEGKV IGRHSGLMYH TIGQRQGLGI GGLKEAGDDP WYVLGKDLQR NVLLVGQGND HPLLFSRALL ASRIYWVNPV ELERPRRLRA KVRYRQSDQD CVLEKTAEGY RAVFDEPQRA VTPGQSVVFY DGDVCLGGGV IETAE

Validated NT Sequence

cttccgccgt ttcgatcacg ccgccgccga ggcagacgtc gccgtcgtag aacaccaccg attgccccgg ggtcactgcg cgctgcggtt cgtcgaacac cgcgcggtag ccttcggcgg ttttctccag gacgcagtcc tggtcgctct gccggtagcg caccttggcc cgcaggcggc gcggccgctc cagttcgacc gggttgaccc agtagattcg cgaggccagc agggcgcggg agaacagcag cgggtggtcg ttgccctggc cgaccagcag cacgttgcgt tgcaggtcct tgccgagcac gtaccaggga tcgtcgccgg cttccttgag accgccgatg ccgaggccct ggcgctggcc gatggtgtgg tacatcaggc cgctgtggcg accgatgacc ttgccttcgg tggtctcgat gtcgcccggc tgggccggca ggtactgctt gaggaaatcg gtgaagcgtc gctcgccaat gaagcagatg cccgtggagt ccttcttctt cgccgtggcg aggccatgct tctcggcgat ggcgcggact tccggcttct ccagttcgcc gacggggaac aggctgcggg cgatctgctc gccgcccacc gcgtgcagga agtagctctg gtccttgttc gggtccaggc ccttgagcag ctcggtgcgc ccgtcgcggt cgcggcgccg cacatagtgg ccggtggcga tcaggtccgc gccgagcatc agcgcgtagt cgaggaacgc cttgaacttg atttcgcggt tgcagaggat gtccgggttc ggcgtacggc cggccttgta ctcggcaagg aagtgctcga acacgttatc ccagtattcc gcggcgaagt tcgccgtgtg cagcttgatg ccgatccggt cgcagacggc ctgggcgtcg gccaggtcct cgcgggcggt acagtattcg gtgccgtcgt cctcgtccca gttcttcatg aacaggcctt cgacctgata gccctgctgc aacaggagca gggcggagac ggacgagtcg acgccgccgg acatgccgac gatcacgcgc tcgcggcgtg gggtgggggc ggattcagac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg aatccgcccc caccccacgc cgcgagcgcg tgatcgtcgg catgtccggc ggcgtcgact cgtccgtctc cgccctgctc ctgttgcagc agggctatca ggtcgaaggc ctgttcatga agaactggga cgaggacgac ggcaccgaat actgtaccgc ccgcgaggac ctggccgacg cccaggccgt ctgcgaccgg atcggcatca agctgcacac ggcgaacttc gccgcggaat actgggataa cgtgttcgag cacttccttg ccgagtacaa ggccggccgt acgccgaacc cggacatcct ctgcaaccgc gaaatcaagt tcaaggcgtt cctcgactac gcgctgatgc tcggcgcgga cctgatcgcc accggccact atgtgcggcg ccgcgaccgc gacgggcgca ccgagctgct caagggcctg gacccgaaca aggaccagag ctacttcctg cacgcggtgg gcggcgagca gatcgcccgc agcctgttcc ccgtcggcga actggagaag ccggaagtcc gcgccatcgc cgagaagcat ggcctcgcca cggcgaagaa gaaggactcc acgggcatct gcttcattgg cgagcgacgc ttcaccgatt tcctcaagca gtacctgccg gcccagccgg gcgacatcga gaccaccgaa ggcaaggtca tcggtcgcca cagcggcctg atgtaccaca ccatcggcca gcgccagggc ctcggcatcg gcggtctcaa ggaagccggc gacgatccct ggtacgtgct cggcaaggac ctgcaacgca acgtgctgct ggtcggccag ggcaacgacc acccgctgct gttctcccgc gccctgctgg cctcgcgaat ctactgggtc aacccggtcg aactggagcg gccgcgccgc ctgcgggcca aggtgcgcta ccggcagagc gaccaggact gcgtcctgga gaaaaccgcc gaaggctacc gcgcggtgtt cgacgaaccg cagcgcgcag tgaccccggg gcaatcggtg gtgttctacg acggcgacgt ctgcctcggc ggcggcgtga tcgaaacggc ggaagcctgg gatttcggag gacgcccgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for PsaeA.17941.a.B1.PW37608

PURIFICATION DATe:	12/2/2014
CONCENTRATION:	20mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG AATCCGCCCC CACCCCACGC CGCGAGCGCG TGATCGTCGG CATGTCCGGC GGCGTCGACT CGTCCGTCTC CGCCCTGCTC CTGTTGCAGC AGGGCTATCA GGTCGAAGGC CTGTTCATGA AGAACTGGGA CGAGGACGAC GGCACCGAAT ACTGTACCGC CCGCGAGGAC CTGGCCGACG CCCAGGCCGT CTGCGACCGG ATCGGCATCA AGCTGCACAC GGCGAACTTC GCCGCGGAAT ACTGGGATAA CGTGTTCGAG CACTTCCTTG CCGAGTACAA GGCCGGCCGT ACGCCGAACC CGGACATCCT CTGCAACCGC GAAATCAAGT TCAAGGCGTT CCTCGACTAC GCGCTGATGC TCGGCGCGGA CCTGATCGCC ACCGGCCACT ATGTGCGGCG CCGCGACCGC GACGGGCGCA CCGAGCTGCT CAAGGGCCTG GACCCGAACA AGGACCAGAG CTACTTCCTG CACGCGGTGG GCGGCGAGCA GATCGCCCGC AGCCTGTTCC CCGTCGGCGA ACTGGAGAAG CCGGAAGTCC GCGCCATCGC CGAGAAGCAT GGCCTCGCCA CGGCGAAGAA GAAGGACTCC ACGGGCATCT GCTTCATTGG CGAGCGACGC TTCACCGATT TCCTCAAGCA GTACCTGCCG GCCCAGCCGG GCGACATCGA GACCACCGAA GGCAAGGTCA TCGGTCGCCA CAGCGGCCTG ATGTACCACA CCATCGGCCA GCGCCAGGGC CTCGGCATCG GCGGTCTCAA GGAAGCCGGC GACGATCCCT GGTACGTGCT CGGCAAGGAC CTGCAACGCA ACGTGCTGCT GGTCGGCCAG GGCAACGACC ACCCGCTGCT GTTCTCCCGC GCCCTGCTGG CCTCGCGAAT CTACTGGGTC AACCCGGTCG AACTGGAGCG GCCGCGCCGC CTGCGGGCCA AGGTGCGCTA CCGGCAGAGC GACCAGGACT GCGTCCTGGA GAAAACCGCC GAAGGCTACC GCGCGGTGTT CGACGAACCG CAGCGCGCAG TGACCCCGGG GCAATCGGTG GTGTTCTACG ACGGCGACGT CTGCCTCGGC GGCGGCGTGA TCGAAACGGC GGAAGCCTGG GATTTCGGAG GACGCCCGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT