PsaeA.17938.a

UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (D-glutamic acid-adding enzyme) (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase) (MurD)

CENTER ID: PsaeA.17938.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.17938.a.B1.GE38488 full length 1 448
PsaeA.17938.a.B2.GE40332 truncation1 9 448
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
PsaeA.17938.a.B2.PS38064 truncation1 9 448

Structures

7SY9
DEPOSITED: 11/24/2021
DETERMINATION: XRay
CLONE: PsaeA.17938.a.B2.GE40332
PROTEIN: PsaeA.17938.a.B2.PS38064
7U35
DEPOSITED: 2/25/2022
DETERMINATION: XRay
CLONE: PsaeA.17938.a.B2.GE40332
PROTEIN: PsaeA.17938.a.B2.PS38065
8DP2
DEPOSITED: 7/14/2022
DETERMINATION: XRay
CLONE: PsaeA.17938.a.B2.GE40332
PROTEIN: PsaeA.17938.a.B2.PS38065

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|208964.12.peg.4623
PGAT: pseudomonas:PA4414
RefSeq: NP_253104.1
UniProt: Q9HVZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLIASDHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL SPACASLDMF KNFEERGRLF AKAVEELA
NT Sequence
atgagcctga tcgcctccga ccacttccgc atcgttgtcg gcctcggcaa gagcggcatg tccctggtgc gctacctggc gcgccgcggc ttgcctttcg ccgtggtcga tacccgagag aacccgccgg agctggccac cctgcgtgcc cagtatccgc aggtggaagt gcgttgcggc gaactcgacg ccgagttcct ctgctccgcc cgcgaactct atgtcagccc cggcttgtcg ctgcgcaccc ctgcgctggt acaggccgcc gcgaaaggcg tgcgcatctc cggtgacatc gatctcttcg cccgcgaggc gaaggccccg atcgtcgcca tcaccggttc caacgcgaag agcaccgtga ccaccctggt gggcgaaatg gcggtggccg cggacaagcg tgtcgccgtc ggcggcaacc tcggcacccc ggcgctcgac ctgctggccg acgacatcga gctgtacgtg ttggagctgt cgagcttcca gctggaaacc tgcgatcgcc tcaacgccga ggtggcgacc gtgctgaacg tcagcgaaga ccatatggat cgctacgacg gcatggctga ctaccacctg gccaagcacc ggatcttccg cggtgcccgc caggtcgtgg tgaatcgcgc cgatgccctg acccgaccgc tgatcgccga taccgtgccg tgctggtcgt tcggcctgaa caagccggac ttcaaggctt tcggcctgat cgaggaagac ggccagaagt ggctggcgtt ccagttcgac aagctgctgc cggttggcga actgaagatc cgtggcgccc acaactattc caacgcgctc gccgcgctgg cgctgggcca tgcggtcggc ctgccgttcg acgccatgct cggcgcgctg aaggcgtttt ccggcctggc tcatcgctgc cagtgggtac gcgagcggca gggcgtgagc tactacgacg attccaaggc caccaacgtc ggcgccgccc tggcggcgat cgaggggctg ggtgccgaca tcgacggcaa gctggtgctg ctcgccggcg gagacggcaa gggcgccgat ttccatgacc tgcgcgagcc ggtcgcgcgc ttctgccggg cggtggtact gcttggccgt gacgccgggc tgattgccca ggcactgggc aacgcggtac cgctggtgcg cgtcgcaacg ctggacgaag cagtccggca ggccgccgag ctggcccgcg aaggcgatgc ggtgctgttg tcgccggcct gcgcgagcct ggacatgttc aagaacttcg aagaacgcgg acgcctgttc gccaaagccg tagaggagct agcgtga
Details for PsaeA.17938.a.B1.GE38488
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS LIASDHFRIV VGLGKSGMSL VRYLARRGLP FAVVDTRENP PELATLRAQY PQVEVRCGEL DAEFLCSARE LYVSPGLSLR TPALVQAAAK GVRISGDIDL FAREAKAPIV AITGSNAKST VTTLVGEMAV AADKRVAVGG NLGTPALDLL ADDIELYVLE LSSFQLETCD RLNAEVATVL NVSEDHMDRY DGMADYHLAK HRIFRGARQV VVNRADALTR PLIADTVPCW SFGLNKPDFK AFGLIEEDGQ KWLAFQFDKL LPVGELKIRG AHNYSNALAA LALGHAVGLP FDAMLGALKA FSGLAHRCQW VRERQGVSYY DDSKATNVGA ALAAIEGLGA DIDGKLVLLA GGDGKGADFH DLREPVARFC RAVVLLGRDA GLIAQALGNA VPLVRVATLD EAVRQAAELA REGDAVLLSP ACASLDMFKN FEERGRLF
Validated NT Sequence
ggcgaacagg cgtccgcgtt cttcgaagtt cttgaacatg tccaggctcg cgcaggccgg cgacaacagc accgcatcgc cttcgcgggc cagctcggcg gcctgccgga ctgcttcgtc cagcgttgcg acgcgcacca gcggtaccgc gttgcccagt gcctgggcaa tcagcccggc gtcacggcca agcagtacca ccgcccggca gaagcgcgcg accggctcgc gcaggtcatg gaaatcggcg cccttgccgt ctccgccggc gagcagcacc agcttgccgt cgatgtcggc acccagcccc tcgatcgccg ccagggcggc gccgacgttg gtggccttgg aatcgtcgta gtagctcacg ccctgccgct cgcgtaccca ctggcagcga tgagccaggc cggaaaacgc cttcagcgcg ccgagcatgg cgtcgaacgg caggccgacc gcatggccca gcgccagcgc ggcgagcgcg ttggaatagt tgtgggcgcc acggatcttc agttcgccaa ccggcagcag cttgtcgaac tggaacgcca gccacttctg gccgtcttcc tcgatcaggc cgaaagcctt gaagtccggc ttgttcaggc cgaacgacca gcacggcacg gtatcggcga tcagcggtcg ggtcagggca tcggcgcgat tcaccacgac ctggcgggca ccgcggaaga tccggtgctt ggccaggtgg tagtcagcca tgccgtcgta gcgatccata tggtcttcgc tgacgttcag cacggtcgcc acctcggcgt tgaggcgatc gcaggtttcc agctggaagc tcgacagctc caacacgtac agctcgatgt cgtcggccag caggtcgagc gccggggtgc cgaggttgcc gccgacggcg acacgcttgt ccgcggccac cgccatttcg cccaccaggg tggtcacggt gctcttcgcg ttggaaccgg tgatggcgac gatcggggcc ttcgcctcgc gggcgaagag atcgatgtca ccggagatgc gcacgccttt cgcggcggcc tgtaccagcg caggggtgcg cagcgacaag ccggggctga catagagttc gcgggcggag cagaggaact cggcgtcgag ttcgccgcaa cgcacttcca cctgcggata ctgggcacgc agggtggcca gctccggcgg gttctctcgg gtatcgacca cggcgaaagg caagccgcgg cgcgccaggt agcgcaccag ggacatgccg ctcttgccga ggccgacaac gatgcggaag tggtcggagg cgatcaggct catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS LIASDHFRIV VGLGKSGMSL VRYLARRGLP FAVVDTRENP PELATLRAQY PQVEVRCGEL DAEFLCSARE LYVSPGLSLR TPALVQAAAK GVRISGDIDL FAREAKAPIV AITGSNAKST VTTLVGEMAV AADKRVAVGG NLGTPALDLL ADDIELYVLE LSSFQLETCD RLNAEVATVL NVSEDHMDRY DGMADYHLAK HRIFRGARQV VVNRADALTR PLIADTVPCW SFGLNKPDFK AFGLIEEDGQ KWLAFQFDKL LPVGELKIRG AHNYSNALAA LALGHAVGLP FDAMLGALKA FSGLAHRCQW VRERQGVSYY DDSKATNVGA ALAAIEGLGA DIDGKLVLLA GGDGKGADFH DLREPVARFC RAVVLLGRDA GLIAQALGNA VPLVRVATLD EAVRQAAELA REGDAVLLSP ACASLDMFKN FEERGRLFAK AVEELA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tgatcgcctc cgaccacttc cgcatcgttg tcggcctcgg caagagcggc atgtccctgg tgcgctacct ggcgcgccgc ggcttgcctt tcgccgtggt cgatacccga gagaacccgc cggagctggc caccctgcgt gcccagtatc cgcaggtgga agtgcgttgc ggcgaactcg acgccgagtt cctctgctcc gcccgcgaac tctatgtcag ccccggcttg tcgctgcgca cccctgcgct ggtacaggcc gccgcgaaag gcgtgcgcat ctccggtgac atcgatctct tcgcccgcga ggcgaaggcc ccgatcgtcg ccatcaccgg ttccaacgcg aagagcaccg tgaccaccct ggtgggcgaa atggcggtgg ccgcggacaa gcgtgtcgcc gtcggcggca acctcggcac cccggcgctc gacctgctgg ccgacgacat cgagctgtac gtgttggagc tgtcgagctt ccagctggaa acctgcgatc gcctcaacgc cgaggtggcg accgtgctga acgtcagcga agaccatatg gatcgctacg acggcatggc tgactaccac ctggccaagc accggatctt ccgcggtgcc cgccaggtcg tggtgaatcg cgccgatgcc ctgacccgac cgctgatcgc cgataccgtg ccgtgctggt cgttcggcct gaacaagccg gacttcaagg ctttcggcct gatcgaggaa gacggccaga agtggctggc gttccagttc gacaagctgc tgccggttgg cgaactgaag atccgtggcg cccacaacta ttccaacgcg ctcgccgcgc tggcgctggg ccatgcggtc ggcctgccgt tcgacgccat gctcggcgcg ctgaaggcgt tttccggcct ggctcatcgc tgccagtggg tacgcgagcg gcagggcgtg agctactacg acgattccaa ggccaccaac gtcggcgccg ccctggcggc gatcgagggg ctgggtgccg acatcgacgg caagctggtg ctgctcgccg gcggagacgg caagggcgcc gatttccatg acctgcgcga gccggtcgcg cgcttctgcc gggcggtggt actgcttggc cgtgacgccg ggctgattgc ccaggcactg ggcaacgcgg taccgctggt gcgcgtcgca acgctggacg aagcagtccg gcaggccgcc gagctggccc gcgaaggcga tgcggtgctg ttgtcgccgg cctgcgcgag cctggacatg ttcaagaact tcgaagaacg cggacgcctg ttcgccaaag ccgtagagga gctagcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for PsaeA.17938.a.B2.GE40332
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF RIVVGLGKSG MSLVRYLARR GLPFAVVDTR ENPPELATLR AQYPQVEVRC GELDAEFLCS ARELYVSPGL SLRTPALVQA AAKGVRISGD IDLFAREAKA PIVAITGSNA KSTVTTLVGE MAVAADKRVA VGGNLGTPAL DLLADDIELY VLELSSFQLE TCDRLNAEVA TVLNVSEDHM DRYDGMADYH LAKHRIFRGA RQVVVNRADA LTRPLIADTV PCWSFGLNKP DFKAFGLIEE DGQKWLAFQF DKLLPVGELK IRGAHNYSNA LAALALGHAV GLPFDAMLGA LKAFSGLAHR CQWVRERQGV SYYDDSKATN VGAALAAIEG LGADIDGKLV LLAGGDGKGA DFHDLREPVA RFCRAVVLLG RDAGLIAQAL GNAVPLVRVA TLDEAVRQAA ELAREGDAVL LSPACASLDM FKNFEERGRL FA
Validated NT Sequence
tggcgaacag gcgtccgcgt tcttcgaagt tcttgaacat gtccaggctc gcgcaggccg gcgacaacag caccgcatcg ccttcgcggg ccagctcggc ggcctgccgg actgcttcgt ccagcgttgc gacgcgcacc agcggtaccg cgttgcccag tgcctgggca atcagcccgg cgtcacggcc aagcagtacc accgcccggc agaagcgcgc gaccggctcg cgcaggtcat ggaaatcggc gcccttgccg tctccgccgg cgagcagcac cagcttgccg tcgatgtcgg cacccagccc ctcgatcgcc gccagggcgg cgccgacgtt ggtggccttg gaatcgtcgt agtagctcac gccctgccgc tcgcgtaccc actggcagcg atgagccagg ccggaaaacg ccttcagcgc gccgagcatg gcgtcgaacg gcaggccgac cgcatggccc agcgccagcg cggcgagcgc gttggaatag ttgtgggcgc cacggatctt cagttcgcca accggcagca gcttgtcgaa ctggaacgcc agccacttct ggccgtcttc ctcgatcagg ccgaaagcct tgaagtccgg cttgttcagg ccgaacgacc agcacggcac ggtatcggcg atcagcggtc gggtcagggc atcggcgcga ttcaccacga cctggcgggc accgcggaag atccggtgct tggccaggtg gtagtcagcc atgccgtcgt agcgatccat atggtcttcg ctgacgttca gcacggtcgc cacctcggcg ttgaggcgat cgcaggtttc cagctggaag ctcgacagct ccaacacgta cagctcgatg tcgtcggcca gcaggtcgag cgccggggtg ccgaggttgc cgccgacggc gacacgcttg tccgcggcca ccgccatttc gcccaccagg gtggtcacgg tgctcttcgc gttggaaccg gtgatggcga cgatcggggc cttcgcctcg cgggcgaaga gatcgatgtc accggagatg cgcacgcctt tcgcggcggc ctgtaccagc gcaggggtgc gcagcgacaa gccggggctg acatagagtt cgcgggcgga gcagaggaac tcggcgtcga gttcgccgca acgcacttcc acctgcggat actgggcacg cagggtggcc agctccggcg ggttctctcg ggtatcgacc acggcgaaag gcaagccgcg gcgcgccagg tagcgcacca gggacatgcc gctcttgccg aggccgacaa cgatgcggaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL SPACASLDMF KNFEERGRLF AKAVEELA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttccgca tcgttgtcgg cctcggcaag agcggcatgt ccctggtgcg ctacctggcg cgccgcggct tgcctttcgc cgtggtcgat acccgagaga acccgccgga gctggccacc ctgcgtgccc agtatccgca ggtggaagtg cgttgcggcg aactcgacgc cgagttcctc tgctccgccc gcgaactcta tgtcagcccc ggcttgtcgc tgcgcacccc tgcgctggta caggccgccg cgaaaggcgt gcgcatctcc ggtgacatcg atctcttcgc ccgcgaggcg aaggccccga tcgtcgccat caccggttcc aacgcgaaga gcaccgtgac caccctggtg ggcgaaatgg cggtggccgc ggacaagcgt gtcgccgtcg gcggcaacct cggcaccccg gcgctcgacc tgctggccga cgacatcgag ctgtacgtgt tggagctgtc gagcttccag ctggaaacct gcgatcgcct caacgccgag gtggcgaccg tgctgaacgt cagcgaagac catatggatc gctacgacgg catggctgac taccacctgg ccaagcaccg gatcttccgc ggtgcccgcc aggtcgtggt gaatcgcgcc gatgccctga cccgaccgct gatcgccgat accgtgccgt gctggtcgtt cggcctgaac aagccggact tcaaggcttt cggcctgatc gaggaagacg gccagaagtg gctggcgttc cagttcgaca agctgctgcc ggttggcgaa ctgaagatcc gtggcgccca caactattcc aacgcgctcg ccgcgctggc gctgggccat gcggtcggcc tgccgttcga cgccatgctc ggcgcgctga aggcgttttc cggcctggct catcgctgcc agtgggtacg cgagcggcag ggcgtgagct actacgacga ttccaaggcc accaacgtcg gcgccgccct ggcggcgatc gaggggctgg gtgccgacat cgacggcaag ctggtgctgc tcgccggcgg agacggcaag ggcgccgatt tccatgacct gcgcgagccg gtcgcgcgct tctgccgggc ggtggtactg cttggccgtg acgccgggct gattgcccag gcactgggca acgcggtacc gctggtgcgc gtcgcaacgc tggacgaagc agtccggcag gccgccgagc tggcccgcga aggcgatgcg gtgctgttgt cgccggcctg cgcgagcctg gacatgttca agaacttcga agaacgcgga cgcctgttcg ccaaagccgt agaggagcta gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for PsaeA.17938.a.B2.PS38064
PURIFICATION DATe: 10/5/2016
CONCENTRATION: 46.5mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF RIVVGLGKSG MSLVRYLARR GLPFAVVDTR ENPPELATLR AQYPQVEVRC GELDAEFLCS ARELYVSPGL SLRTPALVQA AAKGVRISGD IDLFAREAKA PIVAITGSNA KSTVTTLVGE MAVAADKRVA VGGNLGTPAL DLLADDIELY VLELSSFQLE TCDRLNAEVA TVLNVSEDHM DRYDGMADYH LAKHRIFRGA RQVVVNRADA LTRPLIADTV PCWSFGLNKP DFKAFGLIEE DGQKWLAFQF DKLLPVGELK IRGAHNYSNA LAALALGHAV GLPFDAMLGA LKAFSGLAHR CQWVRERQGV SYYDDSKATN VGAALAAIEG LGADIDGKLV LLAGGDGKGA DFHDLREPVA RFCRAVVLLG RDAGLIAQAL GNAVPLVRVA TLDEAVRQAA ELAREGDAVL LSPACASLDM FKNFEERGRL FA
Validated NT Sequence
tggcgaacag gcgtccgcgt tcttcgaagt tcttgaacat gtccaggctc gcgcaggccg gcgacaacag caccgcatcg ccttcgcggg ccagctcggc ggcctgccgg actgcttcgt ccagcgttgc gacgcgcacc agcggtaccg cgttgcccag tgcctgggca atcagcccgg cgtcacggcc aagcagtacc accgcccggc agaagcgcgc gaccggctcg cgcaggtcat ggaaatcggc gcccttgccg tctccgccgg cgagcagcac cagcttgccg tcgatgtcgg cacccagccc ctcgatcgcc gccagggcgg cgccgacgtt ggtggccttg gaatcgtcgt agtagctcac gccctgccgc tcgcgtaccc actggcagcg atgagccagg ccggaaaacg ccttcagcgc gccgagcatg gcgtcgaacg gcaggccgac cgcatggccc agcgccagcg cggcgagcgc gttggaatag ttgtgggcgc cacggatctt cagttcgcca accggcagca gcttgtcgaa ctggaacgcc agccacttct ggccgtcttc ctcgatcagg ccgaaagcct tgaagtccgg cttgttcagg ccgaacgacc agcacggcac ggtatcggcg atcagcggtc gggtcagggc atcggcgcga ttcaccacga cctggcgggc accgcggaag atccggtgct tggccaggtg gtagtcagcc atgccgtcgt agcgatccat atggtcttcg ctgacgttca gcacggtcgc cacctcggcg ttgaggcgat cgcaggtttc cagctggaag ctcgacagct ccaacacgta cagctcgatg tcgtcggcca gcaggtcgag cgccggggtg ccgaggttgc cgccgacggc gacacgcttg tccgcggcca ccgccatttc gcccaccagg gtggtcacgg tgctcttcgc gttggaaccg gtgatggcga cgatcggggc cttcgcctcg cgggcgaaga gatcgatgtc accggagatg cgcacgcctt tcgcggcggc ctgtaccagc gcaggggtgc gcagcgacaa gccggggctg acatagagtt cgcgggcgga gcagaggaac tcggcgtcga gttcgccgca acgcacttcc acctgcggat actgggcacg cagggtggcc agctccggcg ggttctctcg ggtatcgacc acggcgaaag gcaagccgcg gcgcgccagg tagcgcacca gggacatgcc gctcttgccg aggccgacaa cgatgcggaa catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL SPACASLDMF KNFEERGRLF AKAVEELA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTCCGCA TCGTTGTCGG CCTCGGCAAG AGCGGCATGT CCCTGGTGCG CTACCTGGCG CGCCGCGGCT TGCCTTTCGC CGTGGTCGAT ACCCGAGAGA ACCCGCCGGA GCTGGCCACC CTGCGTGCCC AGTATCCGCA GGTGGAAGTG CGTTGCGGCG AACTCGACGC CGAGTTCCTC TGCTCCGCCC GCGAACTCTA TGTCAGCCCC GGCTTGTCGC TGCGCACCCC TGCGCTGGTA CAGGCCGCCG CGAAAGGCGT GCGCATCTCC GGTGACATCG ATCTCTTCGC CCGCGAGGCG AAGGCCCCGA TCGTCGCCAT CACCGGTTCC AACGCGAAGA GCACCGTGAC CACCCTGGTG GGCGAAATGG CGGTGGCCGC GGACAAGCGT GTCGCCGTCG GCGGCAACCT CGGCACCCCG GCGCTCGACC TGCTGGCCGA CGACATCGAG CTGTACGTGT TGGAGCTGTC GAGCTTCCAG CTGGAAACCT GCGATCGCCT CAACGCCGAG GTGGCGACCG TGCTGAACGT CAGCGAAGAC CATATGGATC GCTACGACGG CATGGCTGAC TACCACCTGG CCAAGCACCG GATCTTCCGC GGTGCCCGCC AGGTCGTGGT GAATCGCGCC GATGCCCTGA CCCGACCGCT GATCGCCGAT ACCGTGCCGT GCTGGTCGTT CGGCCTGAAC AAGCCGGACT TCAAGGCTTT CGGCCTGATC GAGGAAGACG GCCAGAAGTG GCTGGCGTTC CAGTTCGACA AGCTGCTGCC GGTTGGCGAA CTGAAGATCC GTGGCGCCCA CAACTATTCC AACGCGCTCG CCGCGCTGGC GCTGGGCCAT GCGGTCGGCC TGCCGTTCGA CGCCATGCTC GGCGCGCTGA AGGCGTTTTC CGGCCTGGCT CATCGCTGCC AGTGGGTACG CGAGCGGCAG GGCGTGAGCT ACTACGACGA TTCCAAGGCC ACCAACGTCG GCGCCGCCCT GGCGGCGATC GAGGGGCTGG GTGCCGACAT CGACGGCAAG CTGGTGCTGC TCGCCGGCGG AGACGGCAAG GGCGCCGATT TCCATGACCT GCGCGAGCCG GTCGCGCGCT TCTGCCGGGC GGTGGTACTG CTTGGCCGTG ACGCCGGGCT GATTGCCCAG GCACTGGGCA ACGCGGTACC GCTGGTGCGC GTCGCAACGC TGGACGAAGC AGTCCGGCAG GCCGCCGAGC TGGCCCGCGA AGGCGATGCG GTGCTGTTGT CGCCGGCCTG CGCGAGCCTG GACATGTTCA AGAACTTCGA AGAACGCGGA CGCCTGTTCG CCAAAGCCGT AGAGGAGCTA GCGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT