PsaeA.17936.a

Fructose-1,6-bisphosphatase class 1 (FBPase class 1) (EC 3.1.3.11) (D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1)

CENTER ID: PsaeA.17936.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17936.a.B1.GE38487 full length 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.5355
PGAT: pseudomonas:PA5110
RefSeq: NP_253797.1
UniProt: Q9HU73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRVTLSRYL IEQTRSHNTP ADLRFLIEVV ARACKEISHA VSKGALGGVL GSMGTENVQG EVQKKLDVMS NEILLEANEW AGNLAGMASE EMDHPYQIPG RYPKGAYLLV FDPLDGSSNI DVNVSVGTIF SVLRCPNEYL NQNDTLREEA FLQPGTTQVA AGYAIYGPQT MLMLTLGNGV KGFTLDRELG SFVLTHDNIS VPESTAEFAI NMSNQRHWEA PVKRYVEELL AGKEGPLGKN YNMRWIASMV ADVHRILTRG GVFMYPRDAR EPEKPGKLRL MYEANPMSFI IEQAGGAATN GTQRILDIKP ENLHQRVAVF LGSKQEVERI TGYHAE
NT Sequence
atgtcccgcg ttaccctgag ccgctatctg atcgagcaga cccgcagcca caacaccccg gccgacctgc gcttcctcat cgaggtcgtg gcgcgtgcct gcaaggaaat cagccatgcg gtgtccaagg gcgcgctggg cggcgtgctc ggcagcatgg gcaccgagaa cgtgcagggc gaggtgcaga agaagctgga cgtgatgtcc aacgagatcc tgctggaagc caacgaatgg gccggcaacc tggccggcat ggcctccgag gagatggacc acccctacca gattccgggg cgctatccga aaggcgccta cctgttggtc ttcgacccgc tggacggctc cagcaacatc gacgtcaacg tctcggtcgg caccatcttc tcggtgctgc gctgccccaa cgagtacctg aaccagaacg ataccctgcg cgaggaagcc ttcctccagc cgggcaccac ccaggtcgcc gccggctacg ccatctacgg cccgcagacc atgctgatgc tgaccctcgg caatggcgtc aagggcttca ccctggatcg cgagctgggc agcttcgtcc tcacccacga caacatcagc gtgccggaaa gcaccgccga gttcgccatc aacatgtcca accagcgcca ctgggaagcg ccggtgaaac gctacgtgga agaactgctg gccggcaagg aaggcccgct gggcaagaac tacaacatgc gctggatcgc ctcgatggtc gccgacgtcc accgcatcct gacccgtggc ggcgtcttca tgtacccgcg cgacgcccgc gagccggaga agcccggcaa gctgcgcctg atgtacgaag ccaacccgat gtcgttcatc atcgagcagg ccggcggcgc cgccaccaac ggcacccagc gcatcctcga catcaagccg gagaacctgc accagcgtgt cgcggtgttc ctcggctcga agcaggaagt cgagcgcatc accggctacc acgcggagta g
Details for PsaeA.17936.a.B1.GE38487
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS RVTLSRYLIE QTRSHNTPAD LRFLIEVVAR ACKEISHAVS KGALGGVLGS MGTENVQGEV QKKLDVMSNE ILLEANEWAG NLAGMASEEM DHPYQIPGRY PKGAYLLVFD PLDGSSNIDV NVSVGTIFSV LRCPNEYLNQ NDTLREEAFL QPGTTQVAAG YAIYGPQTML MLTLGNGVKG FTLDRELGSF VLTHDNISVP ESTAEFAINM SNQRHWEAPV KRYVEELLAG KEGPLGKNYN MRWIASMVAD VHRILTRGGV FMYPRDAREP EKPGKLRLMY EANPMSFIIE QAGGAATNGT QRILDIKPEN LHQRVAVFLG SKQEVE
Validated NT Sequence
gcgctcgact tcctgcttcg agccgaggaa caccgcgaca cgctggtgca ggttctccgg cttgatgtcg aggatgcgct gggtgccgtt ggtggcggcg ccgccggcct gctcgatgat gaacgacatc gggttggctt cgtacatcag gcgcagcttg ccgggcttct ccggctcgcg ggcgtcgcgc gggtacatga agacgccgcc acgggtcagg atgcggtgga cgtcggcgac catcgaggcg atccagcgca tgttgtagtt cttgcccagc gggccttcct tgccggccag cagttcttcc acgtagcgtt tcaccggcgc ttcccagtgg cgctggttgg acatgttgat ggcgaactcg gcggtgcttt ccggcacgct gatgttgtcg tgggtgagga cgaagctgcc cagctcgcga tccagggtga agcccttgac gccattgccg agggtcagca tcagcatggt ctgcgggccg tagatggcgt agccggcggc gacctgggtg gtgcccggct ggaggaaggc ttcctcgcgc agggtatcgt tctggttcag gtactcgttg gggcagcgca gcaccgagaa gatggtgccg accgagacgt tgacgtcgat gttgctggag ccgtccagcg ggtcgaagac caacaggtag gcgcctttcg gatagcgccc cggaatctgg taggggtggt ccatctcctc ggaggccatg ccggccaggt tgccggccca ttcgttggct tccagcagga tctcgttgga catcacgtcc agcttcttct gcacctcgcc ctgcacgttc tcggtgccca tgctgccgag cacgccgccc agcgcgccct tggacaccgc atggctgatt tccttgcagg cacgcgccac gacctcgatg aggaagcgca ggtcggccgg ggtgttgtgg ctgcgggtct gctcgatcag atagcggctc agggtaacgc gggacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS RVTLSRYLIE QTRSHNTPAD LRFLIEVVAR ACKEISHAVS KGALGGVLGS MGTENVQGEV QKKLDVMSNE ILLEANEWAG NLAGMASEEM DHPYQIPGRY PKGAYLLVFD PLDGSSNIDV NVSVGTIFSV LRCPNEYLNQ NDTLREEAFL QPGTTQVAAG YAIYGPQTML MLTLGNGVKG FTLDRELGSF VLTHDNISVP ESTAEFAINM SNQRHWEAPV KRYVEELLAG KEGPLGKNYN MRWIASMVAD VHRILTRGGV FMYPRDAREP EKPGKLRLMY EANPMSFIIE QAGGAATNGT QRILDIKPEN LHQRVAVFLG SKQEVERITG YHAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc gcgttaccct gagccgctat ctgatcgagc agacccgcag ccacaacacc ccggccgacc tgcgcttcct catcgaggtc gtggcgcgtg cctgcaagga aatcagccat gcggtgtcca agggcgcgct gggcggcgtg ctcggcagca tgggcaccga gaacgtgcag ggcgaggtgc agaagaagct ggacgtgatg tccaacgaga tcctgctgga agccaacgaa tgggccggca acctggccgg catggcctcc gaggagatgg accaccccta ccagattccg gggcgctatc cgaaaggcgc ctacctgttg gtcttcgacc cgctggacgg ctccagcaac atcgacgtca acgtctcggt cggcaccatc ttctcggtgc tgcgctgccc caacgagtac ctgaaccaga acgataccct gcgcgaggaa gccttcctcc agccgggcac cacccaggtc gccgccggct acgccatcta cggcccgcag accatgctga tgctgaccct cggcaatggc gtcaagggct tcaccctgga tcgcgagctg ggcagcttcg tcctcaccca cgacaacatc agcgtgccgg aaagcaccgc cgagttcgcc atcaacatgt ccaaccagcg ccactgggaa gcgccggtga aacgctacgt ggaagaactg ctggccggca aggaaggccc gctgggcaag aactacaaca tgcgctggat cgcctcgatg gtcgccgacg tccaccgcat cctgacccgt ggcggcgtct tcatgtaccc gcgcgacgcc cgcgagccgg agaagcccgg caagctgcgc ctgatgtacg aagccaaccc gatgtcgttc atcatcgagc aggccggcgg cgccgccacc aacggcaccc agcgcatcct cgacatcaag ccggagaacc tgcaccagcg tgtcgcggtg ttcctcggct cgaagcagga agtcgagcgc atcaccggct accacgcgga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat