PsaeA.17935.a

60 kDa chaperonin (GroEL protein) (Protein Cpn60)

CENTER ID: PsaeA.17935.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17935.a.B1.GE38486 full length 1 547
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4593
PGAT: pseudomonas:PA4385
RefSeq: NP_253075.1
UniProt: P30718

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAKEVKFGD SARKKMLVGV NVLADAVKAT LGPKGRNVVL DKSFGAPTIT KDGVSVAKEI ELKDKFENMG AQLVKDVASK ANDAAGDGTT TATVLAQAIV NEGLKAVAAG MNPMDLKRGI DKATVAIVAQ LKELAKPCAD TKAIAQVGTI SANSDESIGQ IIAEAMEKVG KEGVITVEEG SGLENELSVV EGMQFDRGYL SPYFVNKPDT MAAELDSPLL LLVDKKISNI REMLPVLEAV AKAGRPLLIV AEDVEGEALA TLVVNNMRGI VKVAAVKAPG FGDRRKAMLQ DIAILTGGTV ISEEVGLSLE GATLEHLGNA KRVVINKENT TIIDGAGVQA DIEARVLQIR KQIEETTSDY DREKLQERLA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVPGGGVALV RALQAIEGLK GDNEEQNVGI ALLRRAVESP LRQIVANAGD EPSVVVDKVK QGSGNYGFNA ATGVYGDMIE MGILDPAKVT RSALQAAASI GGLMITTEAM VAEIVEDKPA MGGMPDMGGM GGMGGMM
NT Sequence
atggctgcca aagaagttaa gttcggcgat tccgctcgca agaaaatgct ggtcggcgtg aacgtgctgg ccgatgccgt caaggccacc ctcggcccga aaggccgcaa cgtggttctg gacaagtcct tcggcgctcc gaccatcacc aaggacggcg tttccgtcgc caaggaaatc gagctgaaag acaagttcga gaacatgggc gcgcaactgg tgaaagacgt tgcctcaagg ccaacggacg ctgccggtga cggcaccacc accgcgaccg tcctggccca ggccatcgtc aacgaaggcc tgaaggccgt tgccgccggc atgaacccga tggacctgaa gcgtggcatc gacaagccca ccgtggccat cgtcgcccag ctgaaagagc tggccaagcc ctggcgcgac accaaggcca tcgcccaggt aggcaccatc tccgccaact ccgacgagtc catcggccag atcattgccg aagccatgga aaaagtcggt aaagaaggcg tgatcaccgt cgaggaaggc tcgggcctgg aaaacgaact gtccgtcgtc gaaggcatgc agttcgatcg cggctacctg tccccctact tcgtgaacaa gccggacacc atggctgccg agctggatag cccgctgctg ctgctggtcg acaagaagat ctccaacatc cgcgaaatgc tgccggtgct ggaagccgtc gccaaggccg gccgtccgct gctgatcgtc gccgaggacg tcgagggcga agccctggcc accctggtgg tgaacaacat gcgtggcatc gtcaaggtcg cggctgtcaa ggctccgggc ttcggcgatc gccgcaaggc catgctgcag gacatcgcca tcctcaccgg cggtaccgtg atcagcgaag aagtcggcct gagcctggaa ggcgctaccc tggagcacct gggcaagccc aagcgcgtcg tgatcaacaa ggaaaacacc accatcatcg atggcgccgg tgtgcaggct gatatcgaag cccgcgtcct gcagatccgc aagcagatcg aggaaaccac ttccgactac gaccgcgaga agctgcaaga gcgcctggcc aagctggccg gcggtgttgc cgtgatcaag gtaggcgctg ccaccgaagt cgagatgaaa gagaagaaag ccccggtcga agacgccctg cacgctaccc gtgcagcggt ggaagagggc gtggttcccg gcggcggcgt agccctggtt cgtgccctgc aagccatcga aggcctgaag ggtgacaacg aggagcagaa cgtcggtatc gccctgctgc gtcgcgccgt cgaatcgccc gtgcgccaga tcgtggccaa cgccggcgac gagccgagcg tagtggtcga caaggtcaag cagggttccg gcaactacgg cttcaacgct gctaccggcg tgtacggcga catgatcgag atgggcatcc tggacccggc caaggtcact cgttccgctc tgcaggctgc ggcctccatc ggcggtctga tgatcaccac cgaagccatg gttgccgaga tcgtggaaga caagccggcc atgggcggca tgcctgacat gggcggcatg ggcggcatgg gcggcatgat gtaa
Details for PsaeA.17935.a.B1.GE38486
HARVESTED ON: 10/9/2014
SEQUENCED ON: 11/24/2014
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA AKEVKFGDSA RKKMLVGVNV LADAVKATLG PKGRNVVLDK SFGAPTITKD GVSVAKEIEL KDKFENMGAQ LVKDVASKAN DAAGDGTTTA TVLAQAIVNE GLKAVAAGMN PMDLKRGIDK ATVAIVAQLK ELAKPCADTK AIAQVGTISA NSDESIGQII AEAMEKVGKE GVITVEEGSG LENELSVVEG MQFDRGYLSP YFVNXXDTMA AELDSPLLLL XXKKISNIRE MLXGAXSRXX XXAVRCXXXX XXXXXXXXXX XXXXXMRGIV KVXAVKAPGF GXRRKAMLQD IAILTGGTVI SEEVGLSLEG ATLEHLGNAK RVVINKENTT IIDGAGVQAD IEARVLQIRK QIEETTSDYD REKLQERLAK LAGGVAVIKV GAATEVEMKE KKARVEDALH ATRAAVEEGV VPGGGVALVR ALQAIEGLKG DNEEQNVGIA LLRRAVESPL RQIVANAGDE PSVVVDKVKQ GSGNYGFNAA TGVYGDMIEM GILDPAKVTR SALQAAASIG GLMITTEAMV AEIVEDKPAM GGMPD
Validated NT Sequence
tgtcaggcat gccgcccatg gccggcttgt cttccacgat ctcggcaacc atggcttcgg tggtgatcat cagaccgccg atggaggccg cagcctgcag agcggaacga gtgaccttgg ccgggtccag gatgcccatc tcgatcatgt cgccgtacac gccggtagca gcgttgaagc cgtagttgcc ggaaccctgc ttgaccttgt cgaccactac gctcggctcg tcgccggcgt tggccacgat ctggcgcagc ggcgattcga cggcgcgacg cagcagggcg ataccgacgt tctgctcctc gttgtcaccc ttcaggcctt cgatggcttg cagggcacga accagggcta cgccgccgcc gggaaccacg ccctcttcca ccgctgcacg ggtagcgtgc agggcgtctt cgacgcgggc tttcttctct ttcatctcga cttcggtggc agcgcctacc ttgatcacgg caacaccgcc ggccagcttg gccaggcgct cttgcagctt ctcgcggtcg tagtcggaag tggtttcctc gatctgcttg cggatctgca ggacgcgggc ttcgatatca gcctgcacac cggcgccatc gatgatggtg gtgttttcct tgttgatcac gacgcgcttg gcgttgccca ggtgctccag ggtagcgcct tccaggctca ggccgacttc ttcgctgatc acggtaccgc cggtgaggat ggcgatgtcc tgcagcatgg ccttgcggcg atngccgaag cccggagcct tgacagcnnn gaccttgacg atgccacgca tnnnnnnnnn nnncannnng nnnannnnnn nnnnnnnnna nnnnnnnnnn nnnnnncagc agcgnacngc cgnnnnnnnn ngacggctnn cagcaccgnn nagcatttcg cggatgttgg agatcttctt gnnnnncagc agcagcagcg ggctatccag ctcggcagcc atggtgtccg nnttgttcac gaagtagggg gacaggtagc cgcgatcgaa ctgcatgcct tcgacgacgg acagttcgtt ttccaggccc gagccttcct cgacggtgat cacgccttct ttaccgactt tttccatggc ttcggcaatg atctggccga tggactcgtc ggagttggcg gagatggtgc ctacctgggc gatggccttg gtgtcggcgc agggcttggc cagctctttc agctgggcga cgatagccac ggtggccttg tcgatgccac gcttcaggtc catcgggttc atgccggcgg caacggcctt caggccttcg ttgacgatgg cctgggccag gacggtcgcg gtggtggtgc cgtcaccggc agcgtcgttg gccttggagg caacgtcttt caccagttgc gcgcccatgt tctcgaactt gtctttcagc tcgatttcct tggcgacgga aacgccgtcc ttggtgatgg tcggagcgcc gaaggacttg tccagaacca cgttgcggcc tttcgggccg agggtggcct tgacggcatc ggccagcacg ttcacgccga ccagcatttt cttgcgagcg gaatcgccga acttaacttc tttggcagcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMA AKEVKFGDSA RKKMLVGVNV LADAVKATLG PKGRNVVLDK SFGAPTITKD GVSVAKEIEL KDKFENMGAQ LVKDVASKAN DAAGDGTTTA TVLAQAIVNE GLKAVAAGMN PMDLKRGIDK ATVAIVAQLK ELAKPCADTK AIAQVGTISA NSDESIGQII AEAMEKVGKE GVITVEEGSG LENELSVVEG MQFDRGYLSP YFVNKPDTMA AELDSPLLLL VDKKISNIRE MLPVLEAVAK AGRPLLIVAE DVEGEALATL VVNNMRGIVK VAAVKAPGFG DRRKAMLQDI AILTGGTVIS EEVGLSLEGA TLEHLGNAKR VVINKENTTI IDGAGVQADI EARVLQIRKQ IEETTSDYDR EKLQERLAKL AGGVAVIKVG AATEVEMKEK KARVEDALHA TRAAVEEGVV PGGGVALVRA LQAIEGLKGD NEEQNVGIAL LRRAVESPLR QIVANAGDEP SVVVDKVKQG SGNYGFNAAT GVYGDMIEMG ILDPAKVTRS ALQAAASIGG LMITTEAMVA EIVEDKPAMG GMPDMGGMGG MGGMM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ccaaagaagt taagttcggc gattccgctc gcaagaaaat gctggtcggc gtgaacgtgc tggccgatgc cgtcaaggcc accctcggcc cgaaaggccg caacgtggtt ctggacaagt ccttcggcgc tccgaccatc accaaggacg gcgtttccgt cgccaaggaa atcgagctga aagacaagtt cgagaacatg ggcgcgcaac tggtgaaaga cgttgcctca aggccaacgg acgctgccgg tgacggcacc accaccgcga ccgtcctggc ccaggccatc gtcaacgaag gcctgaaggc cgttgccgcc ggcatgaacc cgatggacct gaagcgtggc atcgacaagc ccaccgtggc catcgtcgcc cagctgaaag agctggccaa gccctggcgc gacaccaagg ccatcgccca ggtaggcacc atctccgcca actccgacga gtccatcggc cagatcattg ccgaagccat ggaaaaagtc ggtaaagaag gcgtgatcac cgtcgaggaa ggctcgggcc tggaaaacga actgtccgtc gtcgaaggca tgcagttcga tcgcggctac ctgtccccct acttcgtgaa caagccggac accatggctg ccgagctgga tagcccgctg ctgctgctgg tcgacaagaa gatctccaac atccgcgaaa tgctgccggt gctggaagcc gtcgccaagg ccggccgtcc gctgctgatc gtcgccgagg acgtcgaggg cgaagccctg gccaccctgg tggtgaacaa catgcgtggc atcgtcaagg tcgcggctgt caaggctccg ggcttcggcg atcgccgcaa ggccatgctg caggacatcg ccatcctcac cggcggtacc gtgatcagcg aagaagtcgg cctgagcctg gaaggcgcta ccctggagca cctgggcaag cccaagcgcg tcgtgatcaa caaggaaaac accaccatca tcgatggcgc cggtgtgcag gctgatatcg aagcccgcgt cctgcagatc cgcaagcaga tcgaggaaac cacttccgac tacgaccgcg agaagctgca agagcgcctg gccaagctgg ccggcggtgt tgccgtgatc aaggtaggcg ctgccaccga agtcgagatg aaagagaaga aagccccggt cgaagacgcc ctgcacgcta cccgtgcagc ggtggaagag ggcgtggttc ccggcggcgg cgtagccctg gttcgtgccc tgcaagccat cgaaggcctg aagggtgaca acgaggagca gaacgtcggt atcgccctgc tgcgtcgcgc cgtcgaatcg cccgtgcgcc agatcgtggc caacgccggc gacgagccga gcgtagtggt cgacaaggtc aagcagggtt ccggcaacta cggcttcaac gctgctaccg gcgtgtacgg cgacatgatc gagatgggca tcctggaccc ggccaaggtc actcgttccg ctctgcaggc tgcggcctcc atcggcggtc tgatgatcac caccgaagcc atggttgccg agatcgtgga agacaagccg gccatgggcg gcatgcctga catgggcggc atgggcggca tgggcggcat gatgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat