PsaeA.17932.a

Glutamyl-tRNA reductase (GluTR) (EC 1.2.1.70)

CENTER ID: PsaeA.17932.a
ORGANISM: Pseudomonas aeruginosa PAO1
ASSOCIATED DISEASE: Pseudomonas infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
PsaeA.17932.a.B1.GE38382 full length 1 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|208964.12.peg.4888
PGAT: pseudomonas:PA4666
RefSeq: NP_253355.1
UniProt: P42807

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFIALGINH KTASVAVRER VAFTPEQMVE ALQQLCRLTT SREAAILSTC NRSELYLEID HPTADDVLAW LADYHRLTLD ELRACAYVHQ DEDAVRHMMR VASGLDSMVL GEPQILGQMK SAYAVAREAG TVGPLLGRLF QATFSTAKTV RTDTAIGENP VSVAFAAVSL AKQIFSDLHR SQALLIGAGE TITLVARHLF EQGVKRIVVA NRTLERASLL AEQFGAHAVL LSEIPEELAN SDIVISSTAS QLPILGKGAV ERALKQRKHK PMFMVDIAVP RDIEPEVGEL DDVYLYSVDD LHEVVAENLK SRQGAAQAAE ELVGSGVAEF MQRLRELAAV DVLRAYRQQA ERLRDEELGK AQRQLANGAD PAEVMAQLAR GLTNKLLHAP SVQMKKMSAE GRIDALALAQ ELFALDEGAP RH
NT Sequence
ATGGCCTTCA TTGCCCTCGG CATCAATCAC AAGACCGCCT CGGTGGCTGT CCGCGAGCGC GTGGCTTTCA CTCCGGAACA GATGGTCGAG GCGCTGCAGC AGCTCTGCCG CCTGACCACC AGCCGCGAGG CGGCCATCCT GTCGACCTGC AACCGGAGCG AACTCTACCT GGAGATCGAC CATCCGACCG CGGACGACGT GCTCGCCTGG CTGGCGGACT ACCATCGCCT GACTCTCGAC GAGTTGCGCG CCTGTGCCTA TGTGCACCAG GACGAAGACG CGGTGCGCCA CATGATGCGC GTGGCCTCCG GCCTCGACTC GATGGTCCTC GGCGAGCCGC AGATCCTCGG CCAGATGAAG TCGGCCTACG CCGTCGCGCG CGAGGCCGGC ACCGTCGGTC CGCTGCTCGG CCGCCTGTTC CAGGCCACCT TCAGTACGGC GAAGACCGTC CGCACCGATA CCGCCATCGG CGAGAACCCG GTGTCCGTGG CGTTCGCCGC GGTGAGCCTG GCCAAGCAGA TCTTCAGCGA CCTGCACCGC AGCCAGGCGC TGCTGATCGG CGCCGGCGAG ACCATCACCC TGGTCGCGCG CCACCTGTTC GAGCAAGGCG TGAAGCGCAT CGTGGTGGCC AACCGCACCC TGGAGCGCGC CAGCCTGCTC GCCGAGCAGT TCGGCGCCCA TGCGGTGCTG CTTTCGGAGA TCCCCGAGGA GTTGGCCAAC AGCGATATCG TCATCAGCTC CACTGCCAGC CAGTTGCCGA TCCTCGGCAA GGGCGCCGTG GAGCGCGCGC TGAAGCAGCG CAAGCACAAG CCGATGTTCA TGGTCGACAT CGCCGTGCCG CGCGACATCG AGCCGGAAGT CGGCGAACTG GACGACGTCT ACCTCTATAG CGTCGACGAC CTCCACGAAG TAGTGGCGGA GAATCTCAAG AGCCGCCAGG GCGCTGCCCA GGCCGCCGAG GAACTGGTCG GCAGCGGCGT CGCCGAGTTC ATGCAGCGCC TGCGCGAACT GGCCGCGGTG GACGTGCTGC GCGCCTATCG GCAGCAGGCC GAGCGCCTGC GCGATGAGGA GCTGGGCAAG GCCCAGCGGC AACTGGCCAA CGGTGCCGAC CCCGCCGAGG TGATGGCCCA GCTGGCCCGC GGCCTGACCA ACAAACTGCT GCACGCGCCC AGCGTGCAGA TGAAGAAAAT GTCCGCCGAG GGCCGCATCG ACGCGCTGGC CCTGGCCCAG GAGCTGTTCG CCCTCGACGA GGGCGCGCCG CGCCACTGA
Details for PsaeA.17932.a.B1.GE38382
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/28/2014
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMA FIALGINHKT ASVAVRERVA FTPEQMVEAL QQLCRLTTSR EAAILSTCNR SELYLEIDHP TADDVLAWLA DYHRLTLDEL RACAYVHQDE DAVRHMMRVA SGLDSMVLGE PQILGQMKSA YAVAREAGTV GPLLGRLFQA TFSTAKTVRT DTAIGENPVS VAFAAVSLAK QIFSDLHRSQ ALLIGAGETI TLVARHLFEQ GVKRIVVANR TLERASLLAE QFGAHAVLLS EIPEELANSD IVISSTASQL PILGKGAVER ALKQRKHKPM FMVDIAVPRD IEPEVGELDD VYLYSVDDLH EVVAEXLRAA RALXRXXXXG RQRRRXVHAA XXXXXXXTXC AXIXAXXXGX XXXGXXRXXX GXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
cnnnnnnnnn nnncnctana tattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatggc cttcattgcc ctcggcatca atcacaagac cgcctcggtg gctgtccgcg agcgcgtggc tttcactccg gaacagatgg tcgaggcgct gcagcagctc tgccgcctga ccaccagccg cgaggcggcc atcctgtcga cctgcaaccg gagcgaactc tacctggaga tcgaccatcc gaccgcggac gacgtgctcg cctggctggc ggactaccat cgcctgactc tcgacgagtt gcgcgcctgt gcctatgtgc accaggacga agacgcggtg cgccacatga tgcgcgtggc ctccggcctc gactcgatgg tcctcggcga gccgcagatc ctcggccaga tgaagtcggc ctacgccgtc gcgcgcgagg ccggcaccgt cggtccgctg ctcggccgcc tgttccaggc caccttcagt acggcgaaga ccgtccgcac cgataccgcc atcggcgaga acccggtgtc cgtggcgttc gccgcggtga gcctggccaa gcagatcttc agcgacctgc accgcagcca ggcgctgctg atcggcgccg gcgagaccat caccctggtc gcgcgccacc tgttcgagca aggcgtgaag cgcatcgtgg tggccaaccg caccctggag cgcgccagcc tgctcgccga gcagttcggc gcccatgcgg tgctgctttc ggagatcccc gaggagttgg ccaacagcga tatcgtcatc agctccactg ccagccagtt gccgatcctc ggcaagggcg ccgtggagcg cgcgctgaag cagcgcaagc acaagccgat gttcatggtc gacatcgccg tgccgcgcga catcgagccg gaagtcggcg aactggacga cgtctacctc tatagcgtcg acgacctcca cgaagtagtg gcggagnatc tcagagccgc cagggcgctg cncaggcngn nnangantgg tcggcagcgg cgtcgccnag ttcatgcagc nntgcncgan tgnnnnnggn ggacgtnctg cgcgnctatc gnagcngnna nnnnnggcnn atnagnannt ggnnnnnagc gnnactnnnn cgggnncnan nnnnnnngnn nnnnnnncnn gnccnnnngg nnnannnann anntgtgnnn nnnnncccnc
Expected Protein Sequence
MAHHHHHHMA FIALGINHKT ASVAVRERVA FTPEQMVEAL QQLCRLTTSR EAAILSTCNR SELYLEIDHP TADDVLAWLA DYHRLTLDEL RACAYVHQDE DAVRHMMRVA SGLDSMVLGE PQILGQMKSA YAVAREAGTV GPLLGRLFQA TFSTAKTVRT DTAIGENPVS VAFAAVSLAK QIFSDLHRSQ ALLIGAGETI TLVARHLFEQ GVKRIVVANR TLERASLLAE QFGAHAVLLS EIPEELANSD IVISSTASQL PILGKGAVER ALKQRKHKPM FMVDIAVPRD IEPEVGELDD VYLYSVDDLH EVVAENLKSR QGAAQAAEEL VGSGVAEFMQ RLRELAAVDV LRAYRQQAER LRDEELGKAQ RQLANGADPA EVMAQLARGL TNKLLHAPSV QMKKMSAEGR IDALALAQEL FALDEGAPRH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct tcattgccct cggcatcaat cacaagaccg cctcggtggc tgtccgcgag cgcgtggctt tcactccgga acagatggtc gaggcgctgc agcagctctg ccgcctgacc accagccgcg aggcggccat cctgtcgacc tgcaaccgga gcgaactcta cctggagatc gaccatccga ccgcggacga cgtgctcgcc tggctggcgg actaccatcg cctgactctc gacgagttgc gcgcctgtgc ctatgtgcac caggacgaag acgcggtgcg ccacatgatg cgcgtggcct ccggcctcga ctcgatggtc ctcggcgagc cgcagatcct cggccagatg aagtcggcct acgccgtcgc gcgcgaggcc ggcaccgtcg gtccgctgct cggccgcctg ttccaggcca ccttcagtac ggcgaagacc gtccgcaccg ataccgccat cggcgagaac ccggtgtccg tggcgttcgc cgcggtgagc ctggccaagc agatcttcag cgacctgcac cgcagccagg cgctgctgat cggcgccggc gagaccatca ccctggtcgc gcgccacctg ttcgagcaag gcgtgaagcg catcgtggtg gccaaccgca ccctggagcg cgccagcctg ctcgccgagc agttcggcgc ccatgcggtg ctgctttcgg agatccccga ggagttggcc aacagcgata tcgtcatcag ctccactgcc agccagttgc cgatcctcgg caagggcgcc gtggagcgcg cgctgaagca gcgcaagcac aagccgatgt tcatggtcga catcgccgtg ccgcgcgaca tcgagccgga agtcggcgaa ctggacgacg tctacctcta tagcgtcgac gacctccacg aagtagtggc ggagaatctc aagagccgcc agggcgctgc ccaggccgcc gaggaactgg tcggcagcgg cgtcgccgag ttcatgcagc gcctgcgcga actggccgcg gtggacgtgc tgcgcgccta tcggcagcag gccgagcgcc tgcgcgatga ggagctgggc aaggcccagc ggcaactggc caacggtgcc gaccccgccg aggtgatggc ccagctggcc cgcggcctga ccaacaaact gctgcacgcg cccagcgtgc agatgaagaa aatgtccgcc gagggccgca tcgacgcgct ggccctggcc caggagctgt tcgccctcga cgagggcgcg ccgcgccact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat